Amino acid dipepetide frequency for Methanothermus fervidus (strain ATCC 43054 / DSM 2088 / JCM 10308 / V24 S)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
4.636AlaAla: 4.636 ± 0.15
0.857AlaCys: 0.857 ± 0.051
2.827AlaAsp: 2.827 ± 0.076
4.008AlaGlu: 4.008 ± 0.103
2.265AlaPhe: 2.265 ± 0.085
4.313AlaGly: 4.313 ± 0.126
1.079AlaHis: 1.079 ± 0.049
6.027AlaIle: 6.027 ± 0.144
5.835AlaLys: 5.835 ± 0.123
5.801AlaLeu: 5.801 ± 0.139
1.561AlaMet: 1.561 ± 0.05
2.285AlaAsn: 2.285 ± 0.08
1.98AlaPro: 1.98 ± 0.074
1.048AlaGln: 1.048 ± 0.059
2.608AlaArg: 2.608 ± 0.084
3.276AlaSer: 3.276 ± 0.095
2.689AlaThr: 2.689 ± 0.091
4.378AlaVal: 4.378 ± 0.123
0.43AlaTrp: 0.43 ± 0.036
1.9AlaTyr: 1.9 ± 0.073
0.0AlaXaa: 0.0 ± 0.0
Cys
0.678CysAla: 0.678 ± 0.048
0.222CysCys: 0.222 ± 0.025
0.714CysAsp: 0.714 ± 0.041
0.912CysGlu: 0.912 ± 0.053
0.404CysPhe: 0.404 ± 0.033
1.522CysGly: 1.522 ± 0.081
0.253CysHis: 0.253 ± 0.025
1.094CysIle: 1.094 ± 0.053
1.342CysLys: 1.342 ± 0.07
0.891CysLeu: 0.891 ± 0.049
0.268CysMet: 0.268 ± 0.028
0.594CysAsn: 0.594 ± 0.043
0.904CysPro: 0.904 ± 0.062
0.229CysGln: 0.229 ± 0.024
0.474CysArg: 0.474 ± 0.034
0.644CysSer: 0.644 ± 0.041
0.519CysThr: 0.519 ± 0.035
0.876CysVal: 0.876 ± 0.054
0.065CysTrp: 0.065 ± 0.014
0.456CysTyr: 0.456 ± 0.034
0.0CysXaa: 0.0 ± 0.0
Asp
3.156AspAla: 3.156 ± 0.095
0.542AspCys: 0.542 ± 0.034
2.218AspAsp: 2.218 ± 0.066
4.37AspGlu: 4.37 ± 0.113
2.027AspPhe: 2.027 ± 0.076
3.041AspGly: 3.041 ± 0.092
0.777AspHis: 0.777 ± 0.043
5.936AspIle: 5.936 ± 0.113
4.555AspLys: 4.555 ± 0.124
4.672AspLeu: 4.672 ± 0.118
1.136AspMet: 1.136 ± 0.052
1.897AspAsn: 1.897 ± 0.071
2.749AspPro: 2.749 ± 0.082
0.579AspGln: 0.579 ± 0.039
1.72AspArg: 1.72 ± 0.063
2.129AspSer: 2.129 ± 0.074
2.053AspThr: 2.053 ± 0.077
4.683AspVal: 4.683 ± 0.128
0.417AspTrp: 0.417 ± 0.035
1.944AspTyr: 1.944 ± 0.074
0.0AspXaa: 0.0 ± 0.0
Glu
4.05GluAla: 4.05 ± 0.103
0.813GluCys: 0.813 ± 0.047
3.768GluAsp: 3.768 ± 0.097
6.486GluGlu: 6.486 ± 0.183
2.838GluPhe: 2.838 ± 0.08
4.373GluGly: 4.373 ± 0.106
0.967GluHis: 0.967 ± 0.057
8.568GluIle: 8.568 ± 0.168
9.488GluLys: 9.488 ± 0.233
6.796GluLeu: 6.796 ± 0.128
1.894GluMet: 1.894 ± 0.067
4.451GluAsn: 4.451 ± 0.117
1.905GluPro: 1.905 ± 0.078
0.756GluGln: 0.756 ± 0.045
3.065GluArg: 3.065 ± 0.108
3.171GluSer: 3.171 ± 0.103
2.924GluThr: 2.924 ± 0.08
5.293GluVal: 5.293 ± 0.117
0.558GluTrp: 0.558 ± 0.04
2.648GluTyr: 2.648 ± 0.086
0.0GluXaa: 0.0 ± 0.0
Phe
2.241PheAla: 2.241 ± 0.076
0.56PheCys: 0.56 ± 0.044
2.027PheAsp: 2.027 ± 0.067
2.598PheGlu: 2.598 ± 0.089
1.485PhePhe: 1.485 ± 0.071
2.754PheGly: 2.754 ± 0.087
0.618PheHis: 0.618 ± 0.043
3.713PheIle: 3.713 ± 0.111
3.76PheLys: 3.76 ± 0.105
3.846PheLeu: 3.846 ± 0.136
0.876PheMet: 0.876 ± 0.05
1.73PheAsn: 1.73 ± 0.07
1.371PhePro: 1.371 ± 0.057
0.667PheGln: 0.667 ± 0.039
1.522PheArg: 1.522 ± 0.059
2.382PheSer: 2.382 ± 0.083
1.845PheThr: 1.845 ± 0.076
2.637PheVal: 2.637 ± 0.069
0.261PheTrp: 0.261 ± 0.025
1.501PheTyr: 1.501 ± 0.075
0.0PheXaa: 0.0 ± 0.0
Gly
4.427GlyAla: 4.427 ± 0.127
1.068GlyCys: 1.068 ± 0.059
3.028GlyAsp: 3.028 ± 0.098
4.365GlyGlu: 4.365 ± 0.105
2.775GlyPhe: 2.775 ± 0.092
4.636GlyGly: 4.636 ± 0.128
1.287GlyHis: 1.287 ± 0.057
7.278GlyIle: 7.278 ± 0.174
6.781GlyLys: 6.781 ± 0.134
5.126GlyLeu: 5.126 ± 0.127
1.754GlyMet: 1.754 ± 0.076
2.864GlyAsn: 2.864 ± 0.108
2.194GlyPro: 2.194 ± 0.087
1.079GlyGln: 1.079 ± 0.056
2.997GlyArg: 2.997 ± 0.091
3.612GlySer: 3.612 ± 0.103
3.393GlyThr: 3.393 ± 0.119
5.207GlyVal: 5.207 ± 0.117
0.552GlyTrp: 0.552 ± 0.042
2.616GlyTyr: 2.616 ± 0.09
0.0GlyXaa: 0.0 ± 0.0
His
1.131HisAla: 1.131 ± 0.06
0.297HisCys: 0.297 ± 0.03
0.826HisAsp: 0.826 ± 0.046
1.253HisGlu: 1.253 ± 0.059
0.631HisPhe: 0.631 ± 0.043
1.347HisGly: 1.347 ± 0.065
0.352HisHis: 0.352 ± 0.033
1.373HisIle: 1.373 ± 0.055
1.397HisLys: 1.397 ± 0.064
1.509HisLeu: 1.509 ± 0.064
0.386HisMet: 0.386 ± 0.031
0.662HisAsn: 0.662 ± 0.038
1.105HisPro: 1.105 ± 0.057
0.297HisGln: 0.297 ± 0.031
0.761HisArg: 0.761 ± 0.044
0.774HisSer: 0.774 ± 0.046
0.706HisThr: 0.706 ± 0.042
1.313HisVal: 1.313 ± 0.057
0.12HisTrp: 0.12 ± 0.018
0.683HisTyr: 0.683 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
6.163IleAla: 6.163 ± 0.132
1.29IleCys: 1.29 ± 0.062
5.629IleAsp: 5.629 ± 0.126
7.56IleGlu: 7.56 ± 0.13
3.857IlePhe: 3.857 ± 0.126
6.53IleGly: 6.53 ± 0.142
1.756IleHis: 1.756 ± 0.064
9.134IleIle: 9.134 ± 0.188
10.47IleLys: 10.47 ± 0.182
9.298IleLeu: 9.298 ± 0.19
1.918IleMet: 1.918 ± 0.072
4.672IleAsn: 4.672 ± 0.102
4.375IlePro: 4.375 ± 0.104
1.603IleGln: 1.603 ± 0.063
3.818IleArg: 3.818 ± 0.102
5.994IleSer: 5.994 ± 0.124
4.795IleThr: 4.795 ± 0.134
7.5IleVal: 7.5 ± 0.13
0.519IleTrp: 0.519 ± 0.036
3.343IleTyr: 3.343 ± 0.093
0.0IleXaa: 0.0 ± 0.0
Lys
5.134LysAla: 5.134 ± 0.128
1.24LysCys: 1.24 ± 0.062
5.584LysAsp: 5.584 ± 0.121
8.949LysGlu: 8.949 ± 0.207
3.911LysPhe: 3.911 ± 0.107
5.23LysGly: 5.23 ± 0.125
1.673LysHis: 1.673 ± 0.074
12.68LysIle: 12.68 ± 0.259
13.115LysLys: 13.115 ± 0.247
8.902LysLeu: 8.902 ± 0.161
2.556LysMet: 2.556 ± 0.078
6.864LysAsn: 6.864 ± 0.149
3.302LysPro: 3.302 ± 0.112
1.337LysGln: 1.337 ± 0.063
4.459LysArg: 4.459 ± 0.102
4.511LysSer: 4.511 ± 0.116
4.313LysThr: 4.313 ± 0.112
6.859LysVal: 6.859 ± 0.144
0.766LysTrp: 0.766 ± 0.049
4.636LysTyr: 4.636 ± 0.099
0.0LysXaa: 0.0 ± 0.0
Leu
5.715LeuAla: 5.715 ± 0.15
1.045LeuCys: 1.045 ± 0.056
4.784LeuAsp: 4.784 ± 0.117
6.582LeuGlu: 6.582 ± 0.139
3.051LeuPhe: 3.051 ± 0.105
6.275LeuGly: 6.275 ± 0.147
1.537LeuHis: 1.537 ± 0.067
7.69LeuIle: 7.69 ± 0.169
10.16LeuLys: 10.16 ± 0.185
7.578LeuLeu: 7.578 ± 0.197
1.973LeuMet: 1.973 ± 0.082
4.719LeuAsn: 4.719 ± 0.116
3.682LeuPro: 3.682 ± 0.099
1.621LeuGln: 1.621 ± 0.067
3.878LeuArg: 3.878 ± 0.104
5.48LeuSer: 5.48 ± 0.137
4.068LeuThr: 4.068 ± 0.124
5.465LeuVal: 5.465 ± 0.112
0.55LeuTrp: 0.55 ± 0.039
2.689LeuTyr: 2.689 ± 0.096
0.0LeuXaa: 0.0 ± 0.0
Met
1.603MetAla: 1.603 ± 0.068
0.318MetCys: 0.318 ± 0.029
1.246MetAsp: 1.246 ± 0.061
1.85MetGlu: 1.85 ± 0.075
0.818MetPhe: 0.818 ± 0.051
1.412MetGly: 1.412 ± 0.06
0.38MetHis: 0.38 ± 0.032
2.16MetIle: 2.16 ± 0.08
2.788MetLys: 2.788 ± 0.083
1.975MetLeu: 1.975 ± 0.076
0.586MetMet: 0.586 ± 0.041
1.045MetAsn: 1.045 ± 0.046
0.917MetPro: 0.917 ± 0.049
0.446MetGln: 0.446 ± 0.036
1.019MetArg: 1.019 ± 0.056
1.313MetSer: 1.313 ± 0.062
1.024MetThr: 1.024 ± 0.054
1.537MetVal: 1.537 ± 0.059
0.149MetTrp: 0.149 ± 0.018
0.667MetTyr: 0.667 ± 0.042
0.0MetXaa: 0.0 ± 0.0
Asn
2.83AsnAla: 2.83 ± 0.091
0.654AsnCys: 0.654 ± 0.046
1.936AsnAsp: 1.936 ± 0.066
3.341AsnGlu: 3.341 ± 0.109
2.155AsnPhe: 2.155 ± 0.071
2.679AsnGly: 2.679 ± 0.095
0.61AsnHis: 0.61 ± 0.036
5.577AsnIle: 5.577 ± 0.132
5.105AsnLys: 5.105 ± 0.131
4.808AsnLeu: 4.808 ± 0.118
1.035AsnMet: 1.035 ± 0.057
2.382AsnAsn: 2.382 ± 0.103
2.619AsnPro: 2.619 ± 0.087
0.612AsnGln: 0.612 ± 0.046
1.527AsnArg: 1.527 ± 0.068
2.4AsnSer: 2.4 ± 0.092
2.158AsnThr: 2.158 ± 0.086
3.698AsnVal: 3.698 ± 0.106
0.334AsnTrp: 0.334 ± 0.029
1.861AsnTyr: 1.861 ± 0.076
0.0AsnXaa: 0.0 ± 0.0
Pro
2.007ProAla: 2.007 ± 0.072
0.477ProCys: 0.477 ± 0.034
1.975ProAsp: 1.975 ± 0.082
3.411ProGlu: 3.411 ± 0.099
1.577ProPhe: 1.577 ± 0.061
3.075ProGly: 3.075 ± 0.095
0.941ProHis: 0.941 ± 0.049
3.401ProIle: 3.401 ± 0.09
4.029ProLys: 4.029 ± 0.105
3.601ProLeu: 3.601 ± 0.107
0.792ProMet: 0.792 ± 0.049
1.827ProAsn: 1.827 ± 0.071
1.733ProPro: 1.733 ± 0.072
0.863ProGln: 0.863 ± 0.056
1.558ProArg: 1.558 ± 0.063
2.426ProSer: 2.426 ± 0.087
2.228ProThr: 2.228 ± 0.102
2.895ProVal: 2.895 ± 0.081
0.331ProTrp: 0.331 ± 0.029
1.548ProTyr: 1.548 ± 0.068
0.0ProXaa: 0.0 ± 0.0
Gln
1.076GlnAla: 1.076 ± 0.048
0.214GlnCys: 0.214 ± 0.023
0.693GlnAsp: 0.693 ± 0.043
1.188GlnGlu: 1.188 ± 0.051
0.586GlnPhe: 0.586 ± 0.041
1.003GlnGly: 1.003 ± 0.05
0.349GlnHis: 0.349 ± 0.03
1.595GlnIle: 1.595 ± 0.062
1.73GlnLys: 1.73 ± 0.074
1.23GlnLeu: 1.23 ± 0.056
0.516GlnMet: 0.516 ± 0.036
0.852GlnAsn: 0.852 ± 0.048
0.623GlnPro: 0.623 ± 0.041
0.407GlnGln: 0.407 ± 0.04
0.891GlnArg: 0.891 ± 0.054
0.761GlnSer: 0.761 ± 0.045
0.68GlnThr: 0.68 ± 0.051
1.097GlnVal: 1.097 ± 0.048
0.138GlnTrp: 0.138 ± 0.02
0.61GlnTyr: 0.61 ± 0.042
0.0GlnXaa: 0.0 ± 0.0
Arg
2.291ArgAla: 2.291 ± 0.075
0.615ArgCys: 0.615 ± 0.042
2.142ArgAsp: 2.142 ± 0.075
3.416ArgGlu: 3.416 ± 0.099
1.636ArgPhe: 1.636 ± 0.067
2.958ArgGly: 2.958 ± 0.085
0.685ArgHis: 0.685 ± 0.041
3.909ArgIle: 3.909 ± 0.102
4.417ArgLys: 4.417 ± 0.116
3.124ArgLeu: 3.124 ± 0.099
1.115ArgMet: 1.115 ± 0.05
1.832ArgAsn: 1.832 ± 0.07
1.608ArgPro: 1.608 ± 0.068
0.714ArgGln: 0.714 ± 0.038
2.275ArgArg: 2.275 ± 0.082
2.027ArgSer: 2.027 ± 0.078
1.691ArgThr: 1.691 ± 0.068
2.804ArgVal: 2.804 ± 0.087
0.37ArgTrp: 0.37 ± 0.031
1.751ArgTyr: 1.751 ± 0.073
0.0ArgXaa: 0.0 ± 0.0
Ser
2.911SerAla: 2.911 ± 0.091
0.623SerCys: 0.623 ± 0.044
2.468SerAsp: 2.468 ± 0.086
3.58SerGlu: 3.58 ± 0.107
2.184SerPhe: 2.184 ± 0.077
3.943SerGly: 3.943 ± 0.096
0.928SerHis: 0.928 ± 0.049
4.855SerIle: 4.855 ± 0.112
5.793SerLys: 5.793 ± 0.144
4.998SerLeu: 4.998 ± 0.132
1.337SerMet: 1.337 ± 0.055
2.314SerAsn: 2.314 ± 0.085
2.317SerPro: 2.317 ± 0.083
1.233SerGln: 1.233 ± 0.06
2.215SerArg: 2.215 ± 0.076
3.153SerSer: 3.153 ± 0.101
2.59SerThr: 2.59 ± 0.081
3.398SerVal: 3.398 ± 0.098
0.438SerTrp: 0.438 ± 0.035
2.007SerTyr: 2.007 ± 0.072
0.0SerXaa: 0.0 ± 0.0
Thr
2.882ThrAla: 2.882 ± 0.099
0.654ThrCys: 0.654 ± 0.047
2.02ThrAsp: 2.02 ± 0.069
2.356ThrGlu: 2.356 ± 0.083
1.822ThrPhe: 1.822 ± 0.069
3.732ThrGly: 3.732 ± 0.109
0.823ThrHis: 0.823 ± 0.045
4.615ThrIle: 4.615 ± 0.147
3.802ThrLys: 3.802 ± 0.111
4.315ThrLeu: 4.315 ± 0.116
1.022ThrMet: 1.022 ± 0.051
2.038ThrAsn: 2.038 ± 0.082
2.455ThrPro: 2.455 ± 0.1
0.792ThrGln: 0.792 ± 0.045
1.78ThrArg: 1.78 ± 0.065
2.7ThrSer: 2.7 ± 0.089
2.702ThrThr: 2.702 ± 0.113
3.223ThrVal: 3.223 ± 0.142
0.367ThrTrp: 0.367 ± 0.031
1.665ThrTyr: 1.665 ± 0.077
0.0ThrXaa: 0.0 ± 0.0
Val
4.513ValAla: 4.513 ± 0.123
0.925ValCys: 0.925 ± 0.049
4.302ValAsp: 4.302 ± 0.109
5.467ValGlu: 5.467 ± 0.11
2.622ValPhe: 2.622 ± 0.09
5.108ValGly: 5.108 ± 0.118
1.097ValHis: 1.097 ± 0.051
6.551ValIle: 6.551 ± 0.142
7.492ValLys: 7.492 ± 0.152
5.874ValLeu: 5.874 ± 0.124
1.571ValMet: 1.571 ± 0.07
3.124ValAsn: 3.124 ± 0.092
2.919ValPro: 2.919 ± 0.103
1.092ValGln: 1.092 ± 0.055
2.783ValArg: 2.783 ± 0.09
4.175ValSer: 4.175 ± 0.112
3.442ValThr: 3.442 ± 0.125
5.587ValVal: 5.587 ± 0.139
0.487ValTrp: 0.487 ± 0.04
2.405ValTyr: 2.405 ± 0.087
0.0ValXaa: 0.0 ± 0.0
Trp
0.438TrpAla: 0.438 ± 0.041
0.096TrpCys: 0.096 ± 0.018
0.474TrpAsp: 0.474 ± 0.033
0.526TrpGlu: 0.526 ± 0.042
0.305TrpPhe: 0.305 ± 0.029
0.44TrpGly: 0.44 ± 0.039
0.12TrpHis: 0.12 ± 0.019
0.74TrpIle: 0.74 ± 0.042
0.704TrpLys: 0.704 ± 0.049
0.628TrpLeu: 0.628 ± 0.043
0.242TrpMet: 0.242 ± 0.025
0.37TrpAsn: 0.37 ± 0.035
0.222TrpPro: 0.222 ± 0.022
0.19TrpGln: 0.19 ± 0.024
0.352TrpArg: 0.352 ± 0.03
0.328TrpSer: 0.328 ± 0.027
0.279TrpThr: 0.279 ± 0.03
0.456TrpVal: 0.456 ± 0.033
0.083TrpTrp: 0.083 ± 0.016
0.281TrpTyr: 0.281 ± 0.024
0.0TrpXaa: 0.0 ± 0.0
Tyr
1.923TyrAla: 1.923 ± 0.069
0.55TyrCys: 0.55 ± 0.039
1.939TyrAsp: 1.939 ± 0.071
2.59TyrGlu: 2.59 ± 0.084
1.449TyrPhe: 1.449 ± 0.065
2.668TyrGly: 2.668 ± 0.092
0.691TyrHis: 0.691 ± 0.039
3.325TyrIle: 3.325 ± 0.1
3.257TyrLys: 3.257 ± 0.088
3.57TyrLeu: 3.57 ± 0.102
0.714TyrMet: 0.714 ± 0.048
1.795TyrAsn: 1.795 ± 0.083
1.715TyrPro: 1.715 ± 0.073
0.678TyrGln: 0.678 ± 0.044
1.681TyrArg: 1.681 ± 0.074
2.027TyrSer: 2.027 ± 0.066
1.665TyrThr: 1.665 ± 0.076
2.632TyrVal: 2.632 ± 0.084
0.341TyrTrp: 0.341 ± 0.026
1.623TyrTyr: 1.623 ± 0.075
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 1283 proteins (383747 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski