Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Methylococcales; Methylococcaceae; Methylotuvimicrobium; Methylotuvimicrobium alcaliphilum

Average proteome isoelectric point is 6.29

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4041 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G4SZN5|G4SZN5_META2 Uncharacterized protein OS=Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) OX=1091494 GN=MEALZ_2810 PE=4 SV=1
MM1 pKa = 7.18STLKK5 pKa = 9.78TLAAASLIAASGSAAAFTDD24 pKa = 4.64FYY26 pKa = 10.78INNGADD32 pKa = 3.18YY33 pKa = 10.78GGIGTEE39 pKa = 4.11VCPEE43 pKa = 3.69CTGIKK48 pKa = 9.09EE49 pKa = 4.23HH50 pKa = 6.68GAISYY55 pKa = 9.55QSQTLVDD62 pKa = 4.26LQNANQLTVGDD73 pKa = 4.8TITTYY78 pKa = 11.3AGILAGGGNINPQTSGLNQFTAFIPGQPEE107 pKa = 3.73NSNNGLGTNWFMSFYY122 pKa = 8.87TDD124 pKa = 3.33NMKK127 pKa = 9.38GTISDD132 pKa = 4.15LTNPAQPEE140 pKa = 4.03VTYY143 pKa = 10.82TEE145 pKa = 4.12GTINLLYY152 pKa = 10.13STDD155 pKa = 4.23GIDD158 pKa = 3.1WTNFMDD164 pKa = 6.07LIVKK168 pKa = 10.39KK169 pKa = 10.51SVYY172 pKa = 9.32DD173 pKa = 3.55TANNLSITGTVDD185 pKa = 4.05FSDD188 pKa = 4.41VDD190 pKa = 3.8DD191 pKa = 5.21LVYY194 pKa = 10.94KK195 pKa = 10.75DD196 pKa = 4.9LFNFADD202 pKa = 3.9GTSFYY207 pKa = 10.5DD208 pKa = 2.76IWLAGEE214 pKa = 4.74DD215 pKa = 3.85FDD217 pKa = 6.65IPFQLDD223 pKa = 3.18QNLGVPTIDD232 pKa = 4.34FLDD235 pKa = 4.01AGTAKK240 pKa = 10.45IAGTHH245 pKa = 5.87GAQLYY250 pKa = 10.03FDD252 pKa = 4.68TVPEE256 pKa = 4.3PATLALLGFGLLGFAGARR274 pKa = 11.84KK275 pKa = 9.29RR276 pKa = 11.84RR277 pKa = 11.84LAA279 pKa = 3.84

Molecular weight: 29.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G4SY35|G4SY35_META2 Peptide methionine sulfoxide reductase MsrB OS=Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z) OX=1091494 GN=msrB PE=3 SV=1
MM1 pKa = 7.52GWPFLALCAWAPGRR15 pKa = 11.84IFIYY19 pKa = 9.9EE20 pKa = 4.27GYY22 pKa = 10.01IYY24 pKa = 10.44RR25 pKa = 11.84AVNRR29 pKa = 11.84IPALKK34 pKa = 9.79IIEE37 pKa = 4.17QFFRR41 pKa = 11.84MKK43 pKa = 10.69GAMRR47 pKa = 11.84NPSLILNIRR56 pKa = 11.84PSRR59 pKa = 11.84LLLISKK65 pKa = 8.0CRR67 pKa = 11.84CC68 pKa = 3.17

Molecular weight: 7.96 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4041

0

4041

1287047

20

3437

318.5

35.51

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.834 ± 0.037

1.013 ± 0.014

5.753 ± 0.04

6.464 ± 0.031

4.143 ± 0.029

6.885 ± 0.037

2.288 ± 0.019

6.643 ± 0.035

5.036 ± 0.038

10.767 ± 0.044

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.399 ± 0.018

3.888 ± 0.028

4.306 ± 0.025

4.245 ± 0.03

5.61 ± 0.03

6.05 ± 0.03

5.058 ± 0.03

6.445 ± 0.029

1.259 ± 0.015

2.916 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski