Amino acid dipepetide frequency for Methylomicrobium alcaliphilum (strain DSM 19304 / NCIMB 14124 / VKM B-2133 / 20Z)

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
8.335AlaAla: 8.335 ± 0.111
1.018AlaCys: 1.018 ± 0.027
5.015AlaAsp: 5.015 ± 0.064
6.479AlaGlu: 6.479 ± 0.091
3.539AlaPhe: 3.539 ± 0.054
6.602AlaGly: 6.602 ± 0.075
1.796AlaHis: 1.796 ± 0.04
6.067AlaIle: 6.067 ± 0.081
4.435AlaLys: 4.435 ± 0.06
10.2AlaLeu: 10.2 ± 0.1
2.433AlaMet: 2.433 ± 0.042
3.004AlaAsn: 3.004 ± 0.051
3.022AlaPro: 3.022 ± 0.049
3.468AlaGln: 3.468 ± 0.056
4.511AlaArg: 4.511 ± 0.059
4.726AlaSer: 4.726 ± 0.068
3.83AlaThr: 3.83 ± 0.057
6.26AlaVal: 6.26 ± 0.078
1.127AlaTrp: 1.127 ± 0.031
2.468AlaTyr: 2.468 ± 0.047
0.0AlaXaa: 0.0 ± 0.0
Cys
0.828CysAla: 0.828 ± 0.028
0.157CysCys: 0.157 ± 0.011
0.563CysAsp: 0.563 ± 0.022
0.618CysGlu: 0.618 ± 0.023
0.413CysPhe: 0.413 ± 0.018
0.897CysGly: 0.897 ± 0.031
0.329CysHis: 0.329 ± 0.017
0.552CysIle: 0.552 ± 0.022
0.385CysLys: 0.385 ± 0.017
1.107CysLeu: 1.107 ± 0.032
0.188CysMet: 0.188 ± 0.013
0.347CysAsn: 0.347 ± 0.018
0.479CysPro: 0.479 ± 0.02
0.317CysGln: 0.317 ± 0.016
0.73CysArg: 0.73 ± 0.024
0.685CysSer: 0.685 ± 0.025
0.455CysThr: 0.455 ± 0.021
0.629CysVal: 0.629 ± 0.021
0.124CysTrp: 0.124 ± 0.009
0.322CysTyr: 0.322 ± 0.016
0.0CysXaa: 0.0 ± 0.0
Asp
4.856AspAla: 4.856 ± 0.058
0.657AspCys: 0.657 ± 0.021
3.249AspAsp: 3.249 ± 0.058
3.934AspGlu: 3.934 ± 0.064
2.688AspPhe: 2.688 ± 0.05
3.667AspGly: 3.667 ± 0.057
1.211AspHis: 1.211 ± 0.034
4.084AspIle: 4.084 ± 0.053
2.972AspLys: 2.972 ± 0.057
5.904AspLeu: 5.904 ± 0.074
1.284AspMet: 1.284 ± 0.032
2.175AspAsn: 2.175 ± 0.043
2.523AspPro: 2.523 ± 0.05
2.085AspGln: 2.085 ± 0.039
3.416AspArg: 3.416 ± 0.055
3.35AspSer: 3.35 ± 0.052
3.086AspThr: 3.086 ± 0.275
3.31AspVal: 3.31 ± 0.057
0.973AspTrp: 0.973 ± 0.029
2.111AspTyr: 2.111 ± 0.043
0.0AspXaa: 0.0 ± 0.0
Glu
5.952GluAla: 5.952 ± 0.08
0.509GluCys: 0.509 ± 0.022
2.903GluAsp: 2.903 ± 0.051
3.61GluGlu: 3.61 ± 0.077
2.397GluPhe: 2.397 ± 0.045
3.604GluGly: 3.604 ± 0.061
1.698GluHis: 1.698 ± 0.041
4.597GluIle: 4.597 ± 0.058
3.72GluLys: 3.72 ± 0.066
7.068GluLeu: 7.068 ± 0.082
1.639GluMet: 1.639 ± 0.038
2.616GluAsn: 2.616 ± 0.047
2.465GluPro: 2.465 ± 0.038
3.926GluGln: 3.926 ± 0.06
4.12GluArg: 4.12 ± 0.061
3.893GluSer: 3.893 ± 0.049
3.693GluThr: 3.693 ± 0.057
3.806GluVal: 3.806 ± 0.066
0.77GluTrp: 0.77 ± 0.03
1.649GluTyr: 1.649 ± 0.035
0.0GluXaa: 0.0 ± 0.0
Phe
3.45PheAla: 3.45 ± 0.061
0.517PheCys: 0.517 ± 0.021
2.934PheAsp: 2.934 ± 0.051
2.57PheGlu: 2.57 ± 0.047
1.896PhePhe: 1.896 ± 0.044
3.154PheGly: 3.154 ± 0.054
0.873PheHis: 0.873 ± 0.027
2.785PheIle: 2.785 ± 0.051
1.974PheLys: 1.974 ± 0.042
3.856PheLeu: 3.856 ± 0.062
0.937PheMet: 0.937 ± 0.028
1.846PheAsn: 1.846 ± 0.042
1.617PhePro: 1.617 ± 0.038
1.364PheGln: 1.364 ± 0.034
1.978PheArg: 1.978 ± 0.042
3.07PheSer: 3.07 ± 0.054
2.051PheThr: 2.051 ± 0.039
2.601PheVal: 2.601 ± 0.05
0.568PheTrp: 0.568 ± 0.022
1.386PheTyr: 1.386 ± 0.03
0.0PheXaa: 0.0 ± 0.0
Gly
5.185GlyAla: 5.185 ± 0.077
0.9GlyCys: 0.9 ± 0.028
3.654GlyAsp: 3.654 ± 0.065
4.217GlyGlu: 4.217 ± 0.055
3.445GlyPhe: 3.445 ± 0.045
4.963GlyGly: 4.963 ± 0.088
1.632GlyHis: 1.632 ± 0.041
4.938GlyIle: 4.938 ± 0.067
3.951GlyLys: 3.951 ± 0.06
7.595GlyLeu: 7.595 ± 0.091
1.914GlyMet: 1.914 ± 0.044
2.618GlyAsn: 2.618 ± 0.055
1.907GlyPro: 1.907 ± 0.047
2.65GlyGln: 2.65 ± 0.044
3.952GlyArg: 3.952 ± 0.064
4.041GlySer: 4.041 ± 0.06
3.266GlyThr: 3.266 ± 0.054
4.698GlyVal: 4.698 ± 0.06
0.938GlyTrp: 0.938 ± 0.028
2.388GlyTyr: 2.388 ± 0.05
0.0GlyXaa: 0.0 ± 0.0
His
1.951HisAla: 1.951 ± 0.04
0.319HisCys: 0.319 ± 0.017
1.325HisAsp: 1.325 ± 0.032
1.348HisGlu: 1.348 ± 0.031
1.099HisPhe: 1.099 ± 0.029
1.594HisGly: 1.594 ± 0.038
0.677HisHis: 0.677 ± 0.026
1.395HisIle: 1.395 ± 0.035
0.967HisLys: 0.967 ± 0.024
2.347HisLeu: 2.347 ± 0.042
0.46HisMet: 0.46 ± 0.019
0.766HisAsn: 0.766 ± 0.024
1.326HisPro: 1.326 ± 0.033
0.969HisGln: 0.969 ± 0.027
1.376HisArg: 1.376 ± 0.033
1.344HisSer: 1.344 ± 0.033
0.965HisThr: 0.965 ± 0.028
1.303HisVal: 1.303 ± 0.031
0.406HisTrp: 0.406 ± 0.017
0.943HisTyr: 0.943 ± 0.027
0.0HisXaa: 0.0 ± 0.0
Ile
6.851IleAla: 6.851 ± 0.08
0.577IleCys: 0.577 ± 0.024
4.922IleAsp: 4.922 ± 0.079
5.162IleGlu: 5.162 ± 0.063
2.239IlePhe: 2.239 ± 0.044
4.94IleGly: 4.94 ± 0.062
1.406IleHis: 1.406 ± 0.035
3.848IleIle: 3.848 ± 0.062
3.501IleLys: 3.501 ± 0.053
5.864IleLeu: 5.864 ± 0.075
1.289IleMet: 1.289 ± 0.033
2.765IleAsn: 2.765 ± 0.048
3.022IlePro: 3.022 ± 0.05
2.25IleGln: 2.25 ± 0.045
3.527IleArg: 3.527 ± 0.051
3.952IleSer: 3.952 ± 0.057
3.339IleThr: 3.339 ± 0.057
4.496IleVal: 4.496 ± 0.063
0.674IleTrp: 0.674 ± 0.023
1.809IleTyr: 1.809 ± 0.037
0.0IleXaa: 0.0 ± 0.0
Lys
4.624LysAla: 4.624 ± 0.068
0.297LysCys: 0.297 ± 0.017
2.517LysAsp: 2.517 ± 0.048
2.892LysGlu: 2.892 ± 0.053
1.43LysPhe: 1.43 ± 0.035
2.887LysGly: 2.887 ± 0.053
1.298LysHis: 1.298 ± 0.034
3.541LysIle: 3.541 ± 0.057
3.001LysLys: 3.001 ± 0.05
5.203LysLeu: 5.203 ± 0.077
1.225LysMet: 1.225 ± 0.031
2.232LysAsn: 2.232 ± 0.043
2.649LysPro: 2.649 ± 0.045
2.739LysGln: 2.739 ± 0.049
2.911LysArg: 2.911 ± 0.049
3.036LysSer: 3.036 ± 0.061
3.238LysThr: 3.238 ± 0.047
2.997LysVal: 2.997 ± 0.051
0.474LysTrp: 0.474 ± 0.02
1.167LysTyr: 1.167 ± 0.028
0.0LysXaa: 0.0 ± 0.0
Leu
10.045LeuAla: 10.045 ± 0.1
1.091LeuCys: 1.091 ± 0.032
6.307LeuAsp: 6.307 ± 0.075
6.558LeuGlu: 6.558 ± 0.08
4.476LeuPhe: 4.476 ± 0.067
6.765LeuGly: 6.765 ± 0.07
2.274LeuHis: 2.274 ± 0.051
7.008LeuIle: 7.008 ± 0.083
5.817LeuLys: 5.817 ± 0.083
11.505LeuLeu: 11.505 ± 0.119
2.604LeuMet: 2.604 ± 0.049
4.539LeuAsn: 4.539 ± 0.065
5.3LeuPro: 5.3 ± 0.07
4.342LeuGln: 4.342 ± 0.062
5.465LeuArg: 5.465 ± 0.072
7.601LeuSer: 7.601 ± 0.089
6.105LeuThr: 6.105 ± 0.075
6.065LeuVal: 6.065 ± 0.069
1.166LeuTrp: 1.166 ± 0.036
2.639LeuTyr: 2.639 ± 0.044
0.0LeuXaa: 0.0 ± 0.0
Met
2.329MetAla: 2.329 ± 0.046
0.169MetCys: 0.169 ± 0.011
1.166MetAsp: 1.166 ± 0.029
1.183MetGlu: 1.183 ± 0.03
0.695MetPhe: 0.695 ± 0.023
1.519MetGly: 1.519 ± 0.042
0.531MetHis: 0.531 ± 0.02
1.568MetIle: 1.568 ± 0.036
1.299MetLys: 1.299 ± 0.035
2.604MetLeu: 2.604 ± 0.048
0.656MetMet: 0.656 ± 0.026
1.108MetAsn: 1.108 ± 0.027
1.419MetPro: 1.419 ± 0.031
1.124MetGln: 1.124 ± 0.029
1.34MetArg: 1.34 ± 0.032
1.59MetSer: 1.59 ± 0.033
1.687MetThr: 1.687 ± 0.041
1.413MetVal: 1.413 ± 0.038
0.167MetTrp: 0.167 ± 0.012
0.42MetTyr: 0.42 ± 0.017
0.0MetXaa: 0.0 ± 0.0
Asn
3.41AsnAla: 3.41 ± 0.057
0.396AsnCys: 0.396 ± 0.018
2.329AsnAsp: 2.329 ± 0.048
2.419AsnGlu: 2.419 ± 0.045
1.462AsnPhe: 1.462 ± 0.038
2.663AsnGly: 2.663 ± 0.05
0.895AsnHis: 0.895 ± 0.028
2.523AsnIle: 2.523 ± 0.043
1.9AsnLys: 1.9 ± 0.042
3.897AsnLeu: 3.897 ± 0.07
0.804AsnMet: 0.804 ± 0.024
1.625AsnAsn: 1.625 ± 0.035
2.315AsnPro: 2.315 ± 0.046
1.703AsnGln: 1.703 ± 0.036
2.576AsnArg: 2.576 ± 0.054
2.133AsnSer: 2.133 ± 0.047
1.893AsnThr: 1.893 ± 0.043
2.22AsnVal: 2.22 ± 0.049
0.556AsnTrp: 0.556 ± 0.023
1.157AsnTyr: 1.157 ± 0.03
0.0AsnXaa: 0.0 ± 0.0
Pro
3.517ProAla: 3.517 ± 0.053
0.368ProCys: 0.368 ± 0.016
2.978ProAsp: 2.978 ± 0.053
3.794ProGlu: 3.794 ± 0.057
1.89ProPhe: 1.89 ± 0.036
3.266ProGly: 3.266 ± 0.051
0.879ProHis: 0.879 ± 0.025
2.641ProIle: 2.641 ± 0.045
2.041ProLys: 2.041 ± 0.048
4.354ProLeu: 4.354 ± 0.06
1.007ProMet: 1.007 ± 0.032
1.719ProAsn: 1.719 ± 0.039
1.64ProPro: 1.64 ± 0.048
1.438ProGln: 1.438 ± 0.03
1.749ProArg: 1.749 ± 0.038
2.485ProSer: 2.485 ± 0.047
1.876ProThr: 1.876 ± 0.042
3.543ProVal: 3.543 ± 0.058
0.574ProTrp: 0.574 ± 0.021
1.305ProTyr: 1.305 ± 0.033
0.0ProXaa: 0.0 ± 0.0
Gln
4.457GlnAla: 4.457 ± 0.073
0.388GlnCys: 0.388 ± 0.018
1.991GlnAsp: 1.991 ± 0.041
2.455GlnGlu: 2.455 ± 0.048
1.573GlnPhe: 1.573 ± 0.036
2.822GlnGly: 2.822 ± 0.044
1.016GlnHis: 1.016 ± 0.028
2.62GlnIle: 2.62 ± 0.045
1.993GlnLys: 1.993 ± 0.046
4.507GlnLeu: 4.507 ± 0.066
0.999GlnMet: 0.999 ± 0.027
1.634GlnAsn: 1.634 ± 0.036
1.664GlnPro: 1.664 ± 0.038
2.493GlnGln: 2.493 ± 0.056
2.593GlnArg: 2.593 ± 0.048
2.6GlnSer: 2.6 ± 0.049
2.287GlnThr: 2.287 ± 0.046
2.603GlnVal: 2.603 ± 0.05
0.62GlnTrp: 0.62 ± 0.022
1.134GlnTyr: 1.134 ± 0.031
0.0GlnXaa: 0.0 ± 0.0
Arg
3.949ArgAla: 3.949 ± 0.06
0.56ArgCys: 0.56 ± 0.025
2.944ArgAsp: 2.944 ± 0.046
3.512ArgGlu: 3.512 ± 0.059
2.873ArgPhe: 2.873 ± 0.056
2.965ArgGly: 2.965 ± 0.051
1.526ArgHis: 1.526 ± 0.04
4.106ArgIle: 4.106 ± 0.06
2.892ArgLys: 2.892 ± 0.052
6.83ArgLeu: 6.83 ± 0.093
1.444ArgMet: 1.444 ± 0.036
2.263ArgAsn: 2.263 ± 0.038
2.135ArgPro: 2.135 ± 0.042
2.932ArgGln: 2.932 ± 0.052
3.297ArgArg: 3.297 ± 0.065
3.127ArgSer: 3.127 ± 0.051
2.304ArgThr: 2.304 ± 0.042
3.487ArgVal: 3.487 ± 0.062
0.803ArgTrp: 0.803 ± 0.023
2.151ArgTyr: 2.151 ± 0.046
0.0ArgXaa: 0.0 ± 0.0
Ser
5.346SerAla: 5.346 ± 0.069
0.617SerCys: 0.617 ± 0.023
3.447SerAsp: 3.447 ± 0.054
4.019SerGlu: 4.019 ± 0.058
2.465SerPhe: 2.465 ± 0.041
5.191SerGly: 5.191 ± 0.075
1.35SerHis: 1.35 ± 0.029
4.115SerIle: 4.115 ± 0.064
2.701SerLys: 2.701 ± 0.053
6.58SerLeu: 6.58 ± 0.077
1.507SerMet: 1.507 ± 0.034
2.119SerAsn: 2.119 ± 0.045
2.51SerPro: 2.51 ± 0.043
2.147SerGln: 2.147 ± 0.042
3.366SerArg: 3.366 ± 0.053
3.628SerSer: 3.628 ± 0.067
2.816SerThr: 2.816 ± 0.048
4.283SerVal: 4.283 ± 0.058
0.713SerTrp: 0.713 ± 0.024
1.579SerTyr: 1.579 ± 0.035
0.0SerXaa: 0.0 ± 0.0
Thr
4.653ThrAla: 4.653 ± 0.061
0.392ThrCys: 0.392 ± 0.019
3.15ThrAsp: 3.15 ± 0.284
3.336ThrGlu: 3.336 ± 0.051
1.851ThrPhe: 1.851 ± 0.042
4.428ThrGly: 4.428 ± 0.065
1.172ThrHis: 1.172 ± 0.025
3.374ThrIle: 3.374 ± 0.051
1.889ThrLys: 1.889 ± 0.037
5.932ThrLeu: 5.932 ± 0.076
1.065ThrMet: 1.065 ± 0.028
1.536ThrAsn: 1.536 ± 0.042
2.632ThrPro: 2.632 ± 0.049
2.02ThrGln: 2.02 ± 0.042
2.573ThrArg: 2.573 ± 0.045
2.561ThrSer: 2.561 ± 0.042
2.409ThrThr: 2.409 ± 0.049
3.788ThrVal: 3.788 ± 0.064
0.553ThrTrp: 0.553 ± 0.019
1.27ThrTyr: 1.27 ± 0.032
0.0ThrXaa: 0.0 ± 0.0
Val
5.45ValAla: 5.45 ± 0.064
0.685ValCys: 0.685 ± 0.025
4.022ValAsp: 4.022 ± 0.061
4.286ValGlu: 4.286 ± 0.059
2.979ValPhe: 2.979 ± 0.05
4.175ValGly: 4.175 ± 0.062
1.231ValHis: 1.231 ± 0.027
4.426ValIle: 4.426 ± 0.055
3.3ValLys: 3.3 ± 0.049
7.021ValLeu: 7.021 ± 0.072
1.687ValMet: 1.687 ± 0.041
2.538ValAsn: 2.538 ± 0.048
2.539ValPro: 2.539 ± 0.045
2.076ValGln: 2.076 ± 0.036
3.221ValArg: 3.221 ± 0.054
4.21ValSer: 4.21 ± 0.056
3.449ValThr: 3.449 ± 0.061
4.541ValVal: 4.541 ± 0.064
0.726ValTrp: 0.726 ± 0.024
1.883ValTyr: 1.883 ± 0.043
0.0ValXaa: 0.0 ± 0.0
Trp
0.845TrpAla: 0.845 ± 0.027
0.136TrpCys: 0.136 ± 0.011
0.563TrpAsp: 0.563 ± 0.021
0.624TrpGlu: 0.624 ± 0.022
0.571TrpPhe: 0.571 ± 0.019
0.705TrpGly: 0.705 ± 0.026
0.372TrpHis: 0.372 ± 0.019
0.711TrpIle: 0.711 ± 0.026
0.469TrpLys: 0.469 ± 0.02
1.878TrpLeu: 1.878 ± 0.04
0.302TrpMet: 0.302 ± 0.016
0.46TrpAsn: 0.46 ± 0.019
0.547TrpPro: 0.547 ± 0.023
0.87TrpGln: 0.87 ± 0.027
0.932TrpArg: 0.932 ± 0.029
0.715TrpSer: 0.715 ± 0.023
0.548TrpThr: 0.548 ± 0.021
0.827TrpVal: 0.827 ± 0.027
0.177TrpTrp: 0.177 ± 0.011
0.336TrpTyr: 0.336 ± 0.018
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.302TyrAla: 2.302 ± 0.044
0.373TyrCys: 0.373 ± 0.018
1.554TyrAsp: 1.554 ± 0.036
1.621TyrGlu: 1.621 ± 0.037
1.446TyrPhe: 1.446 ± 0.037
2.045TyrGly: 2.045 ± 0.039
0.712TyrHis: 0.712 ± 0.027
1.535TyrIle: 1.535 ± 0.037
1.153TyrLys: 1.153 ± 0.032
3.419TyrLeu: 3.419 ± 0.049
0.542TyrMet: 0.542 ± 0.02
0.966TyrAsn: 0.966 ± 0.03
1.35TyrPro: 1.35 ± 0.035
1.536TyrGln: 1.536 ± 0.032
2.468TyrArg: 2.468 ± 0.049
1.755TyrSer: 1.755 ± 0.04
1.284TyrThr: 1.284 ± 0.034
1.577TyrVal: 1.577 ± 0.034
0.478TyrTrp: 0.478 ± 0.019
1.041TyrTyr: 1.041 ± 0.033
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 4041 proteins (1287047 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski