Desulfotomaculum hydrothermale Lam5 = DSM 18033

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Peptococcaceae; Desulfotomaculum; Desulfotomaculum hydrothermale

Average proteome isoelectric point is 7.0

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2625 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|K8DX46|K8DX46_9FIRM Iron-only hydrogenase maturation protein HydG OS=Desulfotomaculum hydrothermale Lam5 = DSM 18033 OX=1121428 GN=DESHY_10165 PE=4 SV=1
MM1 pKa = 7.33KK2 pKa = 10.44AVVDD6 pKa = 3.61QDD8 pKa = 4.3LCISCGACIDD18 pKa = 3.69VCPEE22 pKa = 3.61VFDD25 pKa = 4.39WNDD28 pKa = 2.98EE29 pKa = 4.23EE30 pKa = 4.32KK31 pKa = 10.99AHH33 pKa = 6.83AIVDD37 pKa = 4.12EE38 pKa = 4.43VPEE41 pKa = 4.34GQEE44 pKa = 3.79DD45 pKa = 3.91QARR48 pKa = 11.84EE49 pKa = 4.0AADD52 pKa = 3.77GCPTEE57 pKa = 4.98AIKK60 pKa = 10.57ISS62 pKa = 3.37

Molecular weight: 6.71 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|K8DY17|K8DY17_9FIRM Histidine kinase OS=Desulfotomaculum hydrothermale Lam5 = DSM 18033 OX=1121428 GN=DESHY_110550 PE=4 SV=1
MM1 pKa = 7.28HH2 pKa = 7.53PLWEE6 pKa = 4.64ALLNLLFPRR15 pKa = 11.84RR16 pKa = 11.84PGCPLCGRR24 pKa = 11.84AGPADD29 pKa = 4.23LCPACRR35 pKa = 11.84QLLADD40 pKa = 4.18WAAKK44 pKa = 8.85PKK46 pKa = 10.52CRR48 pKa = 11.84VCGRR52 pKa = 11.84PLALPEE58 pKa = 4.01QQAVLCGRR66 pKa = 11.84CRR68 pKa = 11.84RR69 pKa = 11.84RR70 pKa = 11.84LPPFEE75 pKa = 4.63LARR78 pKa = 11.84AAGPYY83 pKa = 10.05EE84 pKa = 4.46GGLRR88 pKa = 11.84QAIHH92 pKa = 6.05LLKK95 pKa = 10.65YY96 pKa = 10.28RR97 pKa = 11.84GRR99 pKa = 11.84KK100 pKa = 8.79SLAPLLGQIMAASLPAHH117 pKa = 6.2SPLRR121 pKa = 11.84DD122 pKa = 3.54AQLVLPVPMARR133 pKa = 11.84GRR135 pKa = 11.84LRR137 pKa = 11.84EE138 pKa = 3.84RR139 pKa = 11.84GFNQAEE145 pKa = 4.13LLAASTARR153 pKa = 11.84CLNLPLANRR162 pKa = 11.84LLVKK166 pKa = 10.77SFDD169 pKa = 3.96TPPQTGLNRR178 pKa = 11.84QQRR181 pKa = 11.84QQNLQGAFQVTAPAQIAGRR200 pKa = 11.84RR201 pKa = 11.84ILLVDD206 pKa = 3.91DD207 pKa = 4.45VFTTGATAAAVAGLLKK223 pKa = 10.26QHH225 pKa = 6.54GAAGVYY231 pKa = 10.24VLTAANTEE239 pKa = 3.84QDD241 pKa = 3.45RR242 pKa = 11.84AGSKK246 pKa = 10.38CC247 pKa = 3.34

Molecular weight: 26.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2625

0

2625

769361

16

2039

293.1

32.38

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.542 ± 0.058

1.271 ± 0.024

4.594 ± 0.035

6.338 ± 0.048

3.576 ± 0.031

7.793 ± 0.04

1.915 ± 0.024

6.383 ± 0.043

5.553 ± 0.044

10.828 ± 0.059

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.484 ± 0.022

3.625 ± 0.027

4.475 ± 0.035

4.407 ± 0.041

5.628 ± 0.056

4.732 ± 0.032

5.011 ± 0.029

7.77 ± 0.042

0.967 ± 0.02

3.107 ± 0.031
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski