Amino acid dipepetide frequency for Desulfotomaculum hydrothermale Lam5 = DSM 18033

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	12.555AlaAla: 12.555 ± 0.169	1.235AlaCys: 1.235 ± 0.047	4.655AlaAsp: 4.655 ± 0.08	6.167AlaGlu: 6.167 ± 0.106	2.828AlaPhe: 2.828 ± 0.072	9.409AlaGly: 9.409 ± 0.142	1.486AlaHis: 1.486 ± 0.045	5.168AlaIle: 5.168 ± 0.103	4.671AlaLys: 4.671 ± 0.08	9.634AlaLeu: 9.634 ± 0.128	2.45AlaMet: 2.45 ± 0.063	3.065AlaAsn: 3.065 ± 0.064	2.967AlaPro: 2.967 ± 0.068	3.33AlaGln: 3.33 ± 0.079	6.128AlaArg: 6.128 ± 0.089	3.483AlaSer: 3.483 ± 0.071	4.028AlaThr: 4.028 ± 0.077	8.852AlaVal: 8.852 ± 0.119	0.828AlaTrp: 0.828 ± 0.036	2.48AlaTyr: 2.48 ± 0.056	0.0AlaXaa: 0.0 ± 0.0
Cys	0.862CysAla: 0.862 ± 0.033	0.279CysCys: 0.279 ± 0.022	0.513CysAsp: 0.513 ± 0.028	0.551CysGlu: 0.551 ± 0.028	0.464CysPhe: 0.464 ± 0.026	1.363CysGly: 1.363 ± 0.053	0.337CysHis: 0.337 ± 0.02	0.643CysIle: 0.643 ± 0.03	0.463CysLys: 0.463 ± 0.027	1.38CysLeu: 1.38 ± 0.041	0.259CysMet: 0.259 ± 0.02	0.43CysAsn: 0.43 ± 0.025	0.832CysPro: 0.832 ± 0.037	0.638CysGln: 0.638 ± 0.031	1.057CysArg: 1.057 ± 0.041	0.773CysSer: 0.773 ± 0.033	0.61CysThr: 0.61 ± 0.031	0.682CysVal: 0.682 ± 0.037	0.133CysTrp: 0.133 ± 0.013	0.439CysTyr: 0.439 ± 0.026	0.0CysXaa: 0.0 ± 0.0
Asp	3.377AspAla: 3.377 ± 0.064	0.573AspCys: 0.573 ± 0.027	1.825AspAsp: 1.825 ± 0.063	2.995AspGlu: 2.995 ± 0.073	2.051AspPhe: 2.051 ± 0.058	3.16AspGly: 3.16 ± 0.075	0.888AspHis: 0.888 ± 0.036	3.778AspIle: 3.778 ± 0.072	2.783AspLys: 2.783 ± 0.067	5.103AspLeu: 5.103 ± 0.09	1.232AspMet: 1.232 ± 0.039	1.713AspAsn: 1.713 ± 0.049	2.318AspPro: 2.318 ± 0.052	1.677AspGln: 1.677 ± 0.045	2.823AspArg: 2.823 ± 0.067	1.865AspSer: 1.865 ± 0.043	2.181AspThr: 2.181 ± 0.061	3.205AspVal: 3.205 ± 0.062	0.519AspTrp: 0.519 ± 0.029	1.874AspTyr: 1.874 ± 0.051	0.0AspXaa: 0.0 ± 0.0
Glu	5.85GluAla: 5.85 ± 0.109	0.499GluCys: 0.499 ± 0.025	2.659GluAsp: 2.659 ± 0.07	4.821GluGlu: 4.821 ± 0.102	2.107GluPhe: 2.107 ± 0.055	3.954GluGly: 3.954 ± 0.084	1.08GluHis: 1.08 ± 0.032	5.125GluIle: 5.125 ± 0.083	4.596GluLys: 4.596 ± 0.091	7.005GluLeu: 7.005 ± 0.107	1.76GluMet: 1.76 ± 0.048	2.297GluAsn: 2.297 ± 0.058	2.082GluPro: 2.082 ± 0.052	3.509GluGln: 3.509 ± 0.076	3.547GluArg: 3.547 ± 0.078	2.212GluSer: 2.212 ± 0.05	2.93GluThr: 2.93 ± 0.061	5.081GluVal: 5.081 ± 0.088	0.495GluTrp: 0.495 ± 0.028	1.774GluTyr: 1.774 ± 0.052	0.0GluXaa: 0.0 ± 0.0
Phe	3.201PheAla: 3.201 ± 0.065	0.533PheCys: 0.533 ± 0.029	1.699PheAsp: 1.699 ± 0.044	1.717PheGlu: 1.717 ± 0.047	1.601PhePhe: 1.601 ± 0.047	2.866PheGly: 2.866 ± 0.072	0.633PheHis: 0.633 ± 0.03	2.585PheIle: 2.585 ± 0.065	1.869PheLys: 1.869 ± 0.05	3.743PheLeu: 3.743 ± 0.085	0.948PheMet: 0.948 ± 0.033	1.588PheAsn: 1.588 ± 0.046	1.48PhePro: 1.48 ± 0.041	1.163PheGln: 1.163 ± 0.038	1.717PheArg: 1.717 ± 0.048	2.224PheSer: 2.224 ± 0.057	2.126PheThr: 2.126 ± 0.053	2.371PheVal: 2.371 ± 0.056	0.394PheTrp: 0.394 ± 0.024	1.302PheTyr: 1.302 ± 0.044	0.0PheXaa: 0.0 ± 0.0
Gly	6.184GlyAla: 6.184 ± 0.1	1.339GlyCys: 1.339 ± 0.045	3.351GlyAsp: 3.351 ± 0.073	4.543GlyGlu: 4.543 ± 0.09	2.997GlyPhe: 2.997 ± 0.064	5.957GlyGly: 5.957 ± 0.11	1.51GlyHis: 1.51 ± 0.046	5.497GlyIle: 5.497 ± 0.083	4.648GlyLys: 4.648 ± 0.089	8.819GlyLeu: 8.819 ± 0.127	2.124GlyMet: 2.124 ± 0.057	2.501GlyAsn: 2.501 ± 0.06	2.493GlyPro: 2.493 ± 0.059	3.433GlyGln: 3.433 ± 0.069	4.7GlyArg: 4.7 ± 0.091	4.203GlySer: 4.203 ± 0.082	4.049GlyThr: 4.049 ± 0.083	5.932GlyVal: 5.932 ± 0.107	0.859GlyTrp: 0.859 ± 0.033	2.785GlyTyr: 2.785 ± 0.06	0.001GlyXaa: 0.001 ± 0.001
His	1.352HisAla: 1.352 ± 0.044	0.357HisCys: 0.357 ± 0.024	0.845HisAsp: 0.845 ± 0.033	0.915HisGlu: 0.915 ± 0.034	0.745HisPhe: 0.745 ± 0.038	1.401HisGly: 1.401 ± 0.045	0.559HisHis: 0.559 ± 0.028	1.259HisIle: 1.259 ± 0.047	0.963HisLys: 0.963 ± 0.038	2.145HisLeu: 2.145 ± 0.052	0.478HisMet: 0.478 ± 0.022	0.777HisAsn: 0.777 ± 0.033	1.187HisPro: 1.187 ± 0.036	0.792HisGln: 0.792 ± 0.032	1.38HisArg: 1.38 ± 0.042	0.92HisSer: 0.92 ± 0.039	0.996HisThr: 0.996 ± 0.042	1.13HisVal: 1.13 ± 0.045	0.24HisTrp: 0.24 ± 0.017	0.714HisTyr: 0.714 ± 0.028	0.0HisXaa: 0.0 ± 0.0
Ile	5.568IleAla: 5.568 ± 0.083	0.808IleCys: 0.808 ± 0.035	3.145IleAsp: 3.145 ± 0.064	3.745IleGlu: 3.745 ± 0.08	2.371IlePhe: 2.371 ± 0.066	4.437IleGly: 4.437 ± 0.091	1.182IleHis: 1.182 ± 0.044	4.674IleIle: 4.674 ± 0.09	4.473IleLys: 4.473 ± 0.09	6.261IleLeu: 6.261 ± 0.09	1.617IleMet: 1.617 ± 0.05	3.078IleAsn: 3.078 ± 0.068	3.061IlePro: 3.061 ± 0.061	2.059IleGln: 2.059 ± 0.054	3.226IleArg: 3.226 ± 0.061	3.726IleSer: 3.726 ± 0.071	3.859IleThr: 3.859 ± 0.082	4.1IleVal: 4.1 ± 0.083	0.481IleTrp: 0.481 ± 0.025	1.964IleTyr: 1.964 ± 0.054	0.0IleXaa: 0.0 ± 0.0
Lys	4.636LysAla: 4.636 ± 0.101	0.547LysCys: 0.547 ± 0.029	2.648LysAsp: 2.648 ± 0.064	4.561LysGlu: 4.561 ± 0.082	1.679LysPhe: 1.679 ± 0.049	3.637LysGly: 3.637 ± 0.082	1.003LysHis: 1.003 ± 0.037	4.135LysIle: 4.135 ± 0.072	3.755LysLys: 3.755 ± 0.08	5.596LysLeu: 5.596 ± 0.095	1.475LysMet: 1.475 ± 0.041	2.293LysAsn: 2.293 ± 0.053	2.36LysPro: 2.36 ± 0.057	2.693LysGln: 2.693 ± 0.059	2.566LysArg: 2.566 ± 0.062	2.408LysSer: 2.408 ± 0.058	2.964LysThr: 2.964 ± 0.062	4.252LysVal: 4.252 ± 0.084	0.481LysTrp: 0.481 ± 0.025	1.846LysTyr: 1.846 ± 0.052	0.0LysXaa: 0.0 ± 0.0
Leu	12.763LeuAla: 12.763 ± 0.147	1.158LeuCys: 1.158 ± 0.041	5.024LeuAsp: 5.024 ± 0.088	6.377LeuGlu: 6.377 ± 0.11	3.467LeuPhe: 3.467 ± 0.073	8.102LeuGly: 8.102 ± 0.133	1.999LeuHis: 1.999 ± 0.055	5.887LeuIle: 5.887 ± 0.112	5.75LeuLys: 5.75 ± 0.096	11.916LeuLeu: 11.916 ± 0.157	2.398LeuMet: 2.398 ± 0.056	3.767LeuAsn: 3.767 ± 0.072	5.729LeuPro: 5.729 ± 0.089	4.851LeuGln: 4.851 ± 0.097	5.325LeuArg: 5.325 ± 0.082	5.806LeuSer: 5.806 ± 0.101	6.022LeuThr: 6.022 ± 0.093	8.098LeuVal: 8.098 ± 0.114	0.981LeuTrp: 0.981 ± 0.038	2.861LeuTyr: 2.861 ± 0.06	0.0LeuXaa: 0.0 ± 0.0
Met	2.827MetAla: 2.827 ± 0.063	0.22MetCys: 0.22 ± 0.017	1.14MetAsp: 1.14 ± 0.036	1.672MetGlu: 1.672 ± 0.042	0.824MetPhe: 0.824 ± 0.028	1.907MetGly: 1.907 ± 0.053	0.435MetHis: 0.435 ± 0.027	1.438MetIle: 1.438 ± 0.053	1.432MetLys: 1.432 ± 0.049	2.614MetLeu: 2.614 ± 0.065	0.58MetMet: 0.58 ± 0.032	0.881MetAsn: 0.881 ± 0.035	1.175MetPro: 1.175 ± 0.038	1.088MetGln: 1.088 ± 0.038	1.149MetArg: 1.149 ± 0.036	1.366MetSer: 1.366 ± 0.039	1.384MetThr: 1.384 ± 0.047	1.986MetVal: 1.986 ± 0.05	0.155MetTrp: 0.155 ± 0.016	0.569MetTyr: 0.569 ± 0.026	0.0MetXaa: 0.0 ± 0.0
Asn	2.425AsnAla: 2.425 ± 0.057	0.572AsnCys: 0.572 ± 0.029	1.315AsnAsp: 1.315 ± 0.046	1.726AsnGlu: 1.726 ± 0.05	1.427AsnPhe: 1.427 ± 0.041	2.44AsnGly: 2.44 ± 0.057	0.832AsnHis: 0.832 ± 0.031	2.831AsnIle: 2.831 ± 0.053	2.212AsnLys: 2.212 ± 0.055	4.379AsnLeu: 4.379 ± 0.079	0.949AsnMet: 0.949 ± 0.035	1.601AsnAsn: 1.601 ± 0.053	2.168AsnPro: 2.168 ± 0.056	1.617AsnGln: 1.617 ± 0.048	2.381AsnArg: 2.381 ± 0.07	1.769AsnSer: 1.769 ± 0.046	1.788AsnThr: 1.788 ± 0.048	2.158AsnVal: 2.158 ± 0.057	0.417AsnTrp: 0.417 ± 0.024	1.243AsnTyr: 1.243 ± 0.041	0.0AsnXaa: 0.0 ± 0.0
Pro	5.091ProAla: 5.091 ± 0.099	0.502ProCys: 0.502 ± 0.028	2.529ProAsp: 2.529 ± 0.059	3.417ProGlu: 3.417 ± 0.072	1.661ProPhe: 1.661 ± 0.046	4.379ProGly: 4.379 ± 0.079	0.905ProHis: 0.905 ± 0.037	1.831ProIle: 1.831 ± 0.059	1.779ProLys: 1.779 ± 0.049	4.333ProLeu: 4.333 ± 0.086	0.962ProMet: 0.962 ± 0.032	1.31ProAsn: 1.31 ± 0.042	2.015ProPro: 2.015 ± 0.056	1.773ProGln: 1.773 ± 0.052	2.156ProArg: 2.156 ± 0.049	1.798ProSer: 1.798 ± 0.048	1.827ProThr: 1.827 ± 0.051	4.551ProVal: 4.551 ± 0.087	0.524ProTrp: 0.524 ± 0.029	1.402ProTyr: 1.402 ± 0.044	0.0ProXaa: 0.0 ± 0.0
Gln	4.82GlnAla: 4.82 ± 0.089	0.419GlnCys: 0.419 ± 0.026	1.985GlnAsp: 1.985 ± 0.056	3.418GlnGlu: 3.418 ± 0.079	1.175GlnPhe: 1.175 ± 0.039	3.195GlnGly: 3.195 ± 0.065	0.766GlnHis: 0.766 ± 0.03	2.61GlnIle: 2.61 ± 0.061	2.392GlnLys: 2.392 ± 0.064	4.371GlnLeu: 4.371 ± 0.086	1.123GlnMet: 1.123 ± 0.045	1.435GlnAsn: 1.435 ± 0.047	2.178GlnPro: 2.178 ± 0.057	2.72GlnGln: 2.72 ± 0.082	2.262GlnArg: 2.262 ± 0.058	1.712GlnSer: 1.712 ± 0.046	2.065GlnThr: 2.065 ± 0.058	3.785GlnVal: 3.785 ± 0.076	0.411GlnTrp: 0.411 ± 0.023	1.23GlnTyr: 1.23 ± 0.046	0.001GlnXaa: 0.001 ± 0.001
Arg	4.339ArgAla: 4.339 ± 0.079	0.69ArgCys: 0.69 ± 0.031	2.511ArgAsp: 2.511 ± 0.071	4.166ArgGlu: 4.166 ± 0.084	2.019ArgPhe: 2.019 ± 0.049	3.665ArgGly: 3.665 ± 0.068	1.291ArgHis: 1.291 ± 0.049	3.234ArgIle: 3.234 ± 0.062	2.657ArgLys: 2.657 ± 0.061	6.769ArgLeu: 6.769 ± 0.113	1.353ArgMet: 1.353 ± 0.037	1.691ArgAsn: 1.691 ± 0.045	2.663ArgPro: 2.663 ± 0.063	3.934ArgGln: 3.934 ± 0.096	3.697ArgArg: 3.697 ± 0.105	2.502ArgSer: 2.502 ± 0.058	2.382ArgThr: 2.382 ± 0.059	4.148ArgVal: 4.148 ± 0.079	0.629ArgTrp: 0.629 ± 0.03	1.938ArgTyr: 1.938 ± 0.055	0.0ArgXaa: 0.0 ± 0.0
Ser	3.671SerAla: 3.671 ± 0.084	0.703SerCys: 0.703 ± 0.03	2.035SerAsp: 2.035 ± 0.056	2.726SerGlu: 2.726 ± 0.066	2.045SerPhe: 2.045 ± 0.061	4.14SerGly: 4.14 ± 0.071	1.037SerHis: 1.037 ± 0.038	2.649SerIle: 2.649 ± 0.066	2.275SerLys: 2.275 ± 0.056	5.737SerLeu: 5.737 ± 0.085	1.197SerMet: 1.197 ± 0.039	1.49SerAsn: 1.49 ± 0.045	2.324SerPro: 2.324 ± 0.057	2.072SerGln: 2.072 ± 0.057	3.052SerArg: 3.052 ± 0.069	2.397SerSer: 2.397 ± 0.06	2.197SerThr: 2.197 ± 0.052	3.477SerVal: 3.477 ± 0.063	0.536SerTrp: 0.536 ± 0.028	1.566SerTyr: 1.566 ± 0.042	0.0SerXaa: 0.0 ± 0.0
Thr	5.328ThrAla: 5.328 ± 0.084	0.651ThrCys: 0.651 ± 0.027	2.408ThrAsp: 2.408 ± 0.055	2.779ThrGlu: 2.779 ± 0.055	1.817ThrPhe: 1.817 ± 0.051	5.333ThrGly: 5.333 ± 0.09	0.885ThrHis: 0.885 ± 0.032	2.753ThrIle: 2.753 ± 0.066	2.267ThrLys: 2.267 ± 0.051	5.154ThrLeu: 5.154 ± 0.087	1.179ThrMet: 1.179 ± 0.04	1.685ThrAsn: 1.685 ± 0.058	2.525ThrPro: 2.525 ± 0.054	1.332ThrGln: 1.332 ± 0.038	2.475ThrArg: 2.475 ± 0.047	2.124ThrSer: 2.124 ± 0.051	2.518ThrThr: 2.518 ± 0.064	5.043ThrVal: 5.043 ± 0.099	0.417ThrTrp: 0.417 ± 0.024	1.436ThrTyr: 1.436 ± 0.039	0.0ThrXaa: 0.0 ± 0.0
Val	7.501ValAla: 7.501 ± 0.106	1.015ValCys: 1.015 ± 0.041	3.704ValAsp: 3.704 ± 0.091	4.808ValGlu: 4.808 ± 0.089	2.848ValPhe: 2.848 ± 0.059	5.377ValGly: 5.377 ± 0.094	1.253ValHis: 1.253 ± 0.045	5.489ValIle: 5.489 ± 0.089	4.72ValLys: 4.72 ± 0.084	8.182ValLeu: 8.182 ± 0.105	1.943ValMet: 1.943 ± 0.05	3.11ValAsn: 3.11 ± 0.067	3.333ValPro: 3.333 ± 0.075	2.97ValGln: 2.97 ± 0.064	3.751ValArg: 3.751 ± 0.067	4.141ValSer: 4.141 ± 0.083	4.267ValThr: 4.267 ± 0.081	6.416ValVal: 6.416 ± 0.11	0.694ValTrp: 0.694 ± 0.032	2.173ValTyr: 2.173 ± 0.051	0.001ValXaa: 0.001 ± 0.001
Trp	0.717TrpAla: 0.717 ± 0.035	0.125TrpCys: 0.125 ± 0.014	0.472TrpAsp: 0.472 ± 0.028	0.659TrpGlu: 0.659 ± 0.028	0.37TrpPhe: 0.37 ± 0.024	0.711TrpGly: 0.711 ± 0.028	0.307TrpHis: 0.307 ± 0.026	0.402TrpIle: 0.402 ± 0.025	0.289TrpLys: 0.289 ± 0.022	1.389TrpLeu: 1.389 ± 0.051	0.221TrpMet: 0.221 ± 0.018	0.26TrpAsn: 0.26 ± 0.017	0.45TrpPro: 0.45 ± 0.027	0.868TrpGln: 0.868 ± 0.038	0.589TrpArg: 0.589 ± 0.029	0.471TrpSer: 0.471 ± 0.028	0.326TrpThr: 0.326 ± 0.023	0.589TrpVal: 0.589 ± 0.031	0.148TrpTrp: 0.148 ± 0.015	0.303TrpTyr: 0.303 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.353TyrAla: 2.353 ± 0.052	0.484TyrCys: 0.484 ± 0.023	1.475TyrAsp: 1.475 ± 0.043	1.621TyrGlu: 1.621 ± 0.046	1.266TyrPhe: 1.266 ± 0.044	2.492TyrGly: 2.492 ± 0.056	0.768TyrHis: 0.768 ± 0.032	1.847TyrIle: 1.847 ± 0.05	1.542TyrLys: 1.542 ± 0.043	3.749TyrLeu: 3.749 ± 0.069	0.593TyrMet: 0.593 ± 0.03	1.278TyrAsn: 1.278 ± 0.041	1.405TyrPro: 1.405 ± 0.041	1.552TyrGln: 1.552 ± 0.046	2.297TyrArg: 2.297 ± 0.056	1.423TyrSer: 1.423 ± 0.042	1.59TyrThr: 1.59 ± 0.046	1.844TyrVal: 1.844 ± 0.046	0.324TyrTrp: 0.324 ± 0.02	1.169TyrTyr: 1.169 ± 0.043	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.001XaaGlu: 0.001 ± 0.001	0.0XaaPhe: 0.0 ± 0.0	0.001XaaGly: 0.001 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2625 proteins (769361 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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