Pseudogymnoascus sp. VKM F-4519 (FW-2642)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; sordariomyceta; Leotiomycetes; Leotiomycetes incertae sedis; Pseudeurotiaceae; Pseudogymnoascus; unclassified Pseudogymnoascus

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 10628 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A094HYK1|A0A094HYK1_9PEZI MFS domain-containing protein OS=Pseudogymnoascus sp. VKM F-4519 (FW-2642) OX=1420914 GN=V501_00412 PE=3 SV=1
MM1 pKa = 7.07ATNRR5 pKa = 11.84LTQGVMTMCSSLKK18 pKa = 10.47GFTFGPSQEE27 pKa = 3.95VSRR30 pKa = 11.84IEE32 pKa = 4.11ALSNDD37 pKa = 3.4EE38 pKa = 3.91VRR40 pKa = 11.84LEE42 pKa = 3.92FEE44 pKa = 5.27AIIKK48 pKa = 9.75GDD50 pKa = 3.55SYY52 pKa = 11.57SWHH55 pKa = 7.03SINTLMQSQLDD66 pKa = 3.76EE67 pKa = 4.5GALEE71 pKa = 4.11FTAIDD76 pKa = 3.81GDD78 pKa = 5.62VLFEE82 pKa = 4.39WNNLCDD88 pKa = 4.49EE89 pKa = 4.27KK90 pKa = 11.26RR91 pKa = 11.84HH92 pKa = 5.51WEE94 pKa = 3.94QNGCCSITCDD104 pKa = 3.69EE105 pKa = 5.18FYY107 pKa = 10.76RR108 pKa = 11.84DD109 pKa = 3.88CLINLKK115 pKa = 10.17IMPTKK120 pKa = 10.61AGFGVEE126 pKa = 4.18GQQLVTSNMDD136 pKa = 3.68DD137 pKa = 4.01PNLSSTDD144 pKa = 2.84LWVAPKK150 pKa = 10.61HH151 pKa = 5.15FDD153 pKa = 3.29EE154 pKa = 5.09YY155 pKa = 11.6GSAEE159 pKa = 4.17NVGDD163 pKa = 4.88DD164 pKa = 5.03DD165 pKa = 6.65DD166 pKa = 5.34GSSWDD171 pKa = 3.6FEE173 pKa = 5.72DD174 pKa = 5.55YY175 pKa = 11.45VDD177 pKa = 6.06DD178 pKa = 5.94DD179 pKa = 5.01SEE181 pKa = 5.62DD182 pKa = 3.7DD183 pKa = 3.83YY184 pKa = 12.19EE185 pKa = 4.7EE186 pKa = 4.96IEE188 pKa = 4.47STGFEE193 pKa = 4.35YY194 pKa = 10.74EE195 pKa = 3.88DD196 pKa = 5.26DD197 pKa = 4.35EE198 pKa = 5.6YY199 pKa = 11.97SDD201 pKa = 3.69

Molecular weight: 22.82 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A094H0P9|A0A094H0P9_9PEZI Exocyst complex component Sec10 OS=Pseudogymnoascus sp. VKM F-4519 (FW-2642) OX=1420914 GN=V501_07018 PE=3 SV=1
MM1 pKa = 7.94PNTTMHH7 pKa = 6.98RR8 pKa = 11.84PGNPQPNTNRR18 pKa = 11.84QPHH21 pKa = 4.69NHH23 pKa = 6.26RR24 pKa = 11.84PNRR27 pKa = 11.84DD28 pKa = 3.26LHH30 pKa = 6.08PKK32 pKa = 9.71LRR34 pKa = 11.84PRR36 pKa = 11.84VQPPHH41 pKa = 7.49RR42 pKa = 11.84IPTLRR47 pKa = 11.84PPLLRR52 pKa = 11.84HH53 pKa = 5.91LRR55 pKa = 11.84LLQRR59 pKa = 11.84LLPRR63 pKa = 11.84PNRR66 pKa = 11.84ALLIRR71 pKa = 11.84RR72 pKa = 11.84ATGRR76 pKa = 11.84FLLRR80 pKa = 11.84QRR82 pKa = 11.84QGAAAEE88 pKa = 4.12AALGIGPGLGGGGGLAA104 pKa = 4.15

Molecular weight: 11.62 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

10628

0

10628

5361737

15

5866

504.5

55.58

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.926 ± 0.02

1.2 ± 0.009

5.622 ± 0.016

6.273 ± 0.023

3.647 ± 0.014

7.257 ± 0.023

2.243 ± 0.009

5.101 ± 0.018

4.98 ± 0.022

8.744 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.168 ± 0.008

3.756 ± 0.012

5.898 ± 0.025

3.859 ± 0.017

5.801 ± 0.019

8.054 ± 0.02

6.09 ± 0.018

6.17 ± 0.017

1.429 ± 0.009

2.781 ± 0.012
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski