Janthinobacterium sp. KBS0711
Average proteome isoelectric point is 6.75
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5384 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A0M2WRK1|A0A0M2WRK1_9BURK Hca operon transcriptional activator OS=Janthinobacterium sp. KBS0711 OX=1649647 GN=hcaR_2 PE=3 SV=1
MM1 pKa = 7.1 NAVAEE6 pKa = 4.3 MQDD9 pKa = 4.15 VIPSPIIFTDD19 pKa = 3.6 SAAEE23 pKa = 4.01 KK24 pKa = 9.59 VAQLIEE30 pKa = 4.38 EE31 pKa = 4.51 EE32 pKa = 4.63 GNPDD36 pKa = 2.85 LKK38 pKa = 11.13 LRR40 pKa = 11.84 VFVQGGGCSGFQYY53 pKa = 10.94 GFTFDD58 pKa = 4.69 EE59 pKa = 4.93 IVNEE63 pKa = 4.83 DD64 pKa = 3.85 DD65 pKa = 3.11 TTMVKK70 pKa = 10.58 NGVQLLIDD78 pKa = 3.77 SMSYY82 pKa = 10.23 QYY84 pKa = 11.55 LVGAEE89 pKa = 4.01 IDD91 pKa = 3.89 YY92 pKa = 11.28 KK93 pKa = 11.39 DD94 pKa = 4.47 DD95 pKa = 4.08 LEE97 pKa = 4.56 GAQFVIKK104 pKa = 10.68 NPTATSTCGCGSSFSVV120 pKa = 3.54
Molecular weight: 12.98 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.752
IPC2_protein 3.834
IPC_protein 3.77
Toseland 3.567
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.923
Wikipedia 3.694
Rodwell 3.605
Grimsley 3.49
Solomon 3.745
Lehninger 3.694
Nozaki 3.884
DTASelect 4.075
Thurlkill 3.63
EMBOSS 3.706
Sillero 3.897
Patrickios 1.875
IPC_peptide 3.745
IPC2_peptide 3.872
IPC2.peptide.svr19 3.808
Protein with the highest isoelectric point:
>tr|A0A0M2WPU7|A0A0M2WPU7_9BURK Inosine 5'-monophosphate dehydrogenase OS=Janthinobacterium sp. KBS0711 OX=1649647 GN=VM94_01574 PE=4 SV=1
MM1 pKa = 7.91 PKK3 pKa = 9.63 MKK5 pKa = 9.83 TKK7 pKa = 10.72 SSAKK11 pKa = 9.15 KK12 pKa = 9.67 RR13 pKa = 11.84 FRR15 pKa = 11.84 VRR17 pKa = 11.84 PGGTVKK23 pKa = 10.48 SGHH26 pKa = 5.47 AFKK29 pKa = 10.85 RR30 pKa = 11.84 HH31 pKa = 5.44 ILTKK35 pKa = 9.78 KK36 pKa = 3.98 TTKK39 pKa = 10.23 NKK41 pKa = 9.21 RR42 pKa = 11.84 QLRR45 pKa = 11.84 GTRR48 pKa = 11.84 NINASDD54 pKa = 3.65 VTSVMRR60 pKa = 11.84 MMPTAA65 pKa = 4.09
Molecular weight: 7.38 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.358
IPC2_protein 10.789
IPC_protein 12.325
Toseland 12.501
ProMoST 12.983
Dawson 12.515
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.427
Grimsley 12.544
Solomon 12.983
Lehninger 12.896
Nozaki 12.501
DTASelect 12.486
Thurlkill 12.501
EMBOSS 12.998
Sillero 12.501
Patrickios 12.149
IPC_peptide 12.998
IPC2_peptide 11.974
IPC2.peptide.svr19 9.0
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5384
0
5384
1820683
29
4558
338.2
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.81 ± 0.048
0.9 ± 0.01
5.278 ± 0.023
4.897 ± 0.031
3.549 ± 0.021
8.09 ± 0.037
2.218 ± 0.016
4.764 ± 0.027
3.629 ± 0.03
10.912 ± 0.044
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.574 ± 0.017
2.976 ± 0.021
4.906 ± 0.027
4.334 ± 0.023
6.124 ± 0.034
5.693 ± 0.029
5.207 ± 0.027
7.197 ± 0.027
1.341 ± 0.014
2.566 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here