Franconibacter pulveris

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Enterobacteriaceae; Franconibacter

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4200 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A0J8VPE5|A0A0J8VPE5_9ENTR Galactonate transporter OS=Franconibacter pulveris OX=435910 GN=ACH50_11785 PE=4 SV=1
MM1 pKa = 7.64SDD3 pKa = 4.45DD4 pKa = 3.53VALPLQFTDD13 pKa = 3.4AAANKK18 pKa = 8.91VKK20 pKa = 10.8SLIADD25 pKa = 3.6EE26 pKa = 4.73EE27 pKa = 4.41NPNLKK32 pKa = 10.23LRR34 pKa = 11.84VYY36 pKa = 9.05ITGGGCSGFQYY47 pKa = 11.03GFTFDD52 pKa = 4.96DD53 pKa = 4.21QINDD57 pKa = 3.16GDD59 pKa = 4.0MTIEE63 pKa = 4.01KK64 pKa = 10.23QGVALVVDD72 pKa = 4.55PMSLQYY78 pKa = 11.16LVGGSVDD85 pKa = 3.52YY86 pKa = 11.39TEE88 pKa = 4.94GLEE91 pKa = 4.07GSRR94 pKa = 11.84FVVSNPNATSTCGCGSSFSII114 pKa = 5.02

Molecular weight: 12.07 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A0J8VQ41|A0A0J8VQ41_9ENTR Uncharacterized protein OS=Franconibacter pulveris OX=435910 GN=ACH50_10735 PE=4 SV=1
MM1 pKa = 7.53KK2 pKa = 9.82PHH4 pKa = 7.35ASPFRR9 pKa = 11.84AQQRR13 pKa = 11.84LRR15 pKa = 11.84LRR17 pKa = 11.84RR18 pKa = 11.84MMMIRR23 pKa = 11.84HH24 pKa = 7.2LIRR27 pKa = 11.84RR28 pKa = 11.84DD29 pKa = 3.41KK30 pKa = 10.93TPLAILLMAAVVGTLAGLAGVAFEE54 pKa = 4.86RR55 pKa = 11.84AVGWVQQNRR64 pKa = 11.84IGMLAQVADD73 pKa = 4.8RR74 pKa = 11.84AWLVWPLAFIASALLAMVGYY94 pKa = 9.2WLVRR98 pKa = 11.84RR99 pKa = 11.84FAPEE103 pKa = 3.65AGGSGIPEE111 pKa = 3.57IEE113 pKa = 4.21GALEE117 pKa = 3.68EE118 pKa = 4.25LRR120 pKa = 11.84PVRR123 pKa = 11.84WWRR126 pKa = 11.84VLPVKK131 pKa = 10.44FIGGMGTLGAGMVLGRR147 pKa = 11.84EE148 pKa = 4.45GPTVQIGGNIGRR160 pKa = 11.84MVLDD164 pKa = 3.72MFRR167 pKa = 11.84QRR169 pKa = 11.84GAEE172 pKa = 3.86ARR174 pKa = 11.84HH175 pKa = 5.45TLLATGAAAGLSAAFNAPLAGILFIIEE202 pKa = 3.72EE203 pKa = 4.14MRR205 pKa = 11.84PQFRR209 pKa = 11.84YY210 pKa = 10.53NLISIKK216 pKa = 10.66AVFTGVIMSSIVFRR230 pKa = 11.84LFNGEE235 pKa = 3.82GAVIEE240 pKa = 4.32VGRR243 pKa = 11.84LTNAPVNTLWLYY255 pKa = 11.53LMLGIIFGAVGPLFNHH271 pKa = 7.47LIFRR275 pKa = 11.84AQDD278 pKa = 3.06MFQRR282 pKa = 11.84IHH284 pKa = 6.74GGNITKK290 pKa = 9.5WVLVGGALGGLCGVLALIEE309 pKa = 3.96PAAAGGGFNLIPIAAAGNFSVGMLLFIFVARR340 pKa = 11.84VITTLLCFSSGAPGGIFAPMLALGTLLGTAFGMACAAWFPAYY382 pKa = 9.97HH383 pKa = 6.6LEE385 pKa = 4.38AGTFAIAGMGALFAASVRR403 pKa = 11.84APLTGIVLVLEE414 pKa = 4.3MTDD417 pKa = 3.41NYY419 pKa = 10.98QLILPMIITCLGATLLAQFLGGKK442 pKa = 8.62PLYY445 pKa = 10.55SSILARR451 pKa = 11.84TLAKK455 pKa = 10.15QQAEE459 pKa = 4.22QQAQAGQNTT468 pKa = 3.78

Molecular weight: 49.94 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4200

0

4200

1348315

29

4142

321.0

35.42

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.511 ± 0.045

1.024 ± 0.013

5.021 ± 0.03

5.791 ± 0.039

3.817 ± 0.025

7.534 ± 0.041

2.213 ± 0.021

5.317 ± 0.031

3.994 ± 0.036

11.044 ± 0.048

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.64 ± 0.02

3.602 ± 0.028

4.619 ± 0.03

4.554 ± 0.031

5.968 ± 0.034

5.656 ± 0.029

5.277 ± 0.034

7.118 ± 0.032

1.567 ± 0.019

2.733 ± 0.02
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski