Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Fungi incertae sedis; Mucoromycota; Mucoromycotina; Mucoromycetes; Mucorales; Phycomycetaceae; Phycomyces; Phycomyces blakesleeanus

Average proteome isoelectric point is 6.88

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 16155 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A162ZRP6|A0A162ZRP6_PHYB8 Transcription factor E2F/dimerization partner OS=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) OX=763407 GN=PHYBLDRAFT_188617 PE=3 SV=1
MM1 pKa = 7.62LSYY4 pKa = 10.19PIQLALQTLCLASAFLCALLFAVISSLPVPSVLSMGTSSYY44 pKa = 11.06RR45 pKa = 11.84MRR47 pKa = 11.84IIHH50 pKa = 6.87KK51 pKa = 10.26DD52 pKa = 3.35SEE54 pKa = 4.51GCLQSFCTSSEE65 pKa = 4.11VFSSGFLRR73 pKa = 11.84CGSLGKK79 pKa = 8.42TLGSMSCPAVSSEE92 pKa = 3.97IKK94 pKa = 10.46SAGDD98 pKa = 3.27VEE100 pKa = 4.5GLSRR104 pKa = 11.84STSSNQQNSICHH116 pKa = 6.0RR117 pKa = 11.84MNSTVRR123 pKa = 11.84EE124 pKa = 4.13DD125 pKa = 4.07DD126 pKa = 3.34KK127 pKa = 11.05HH128 pKa = 7.65SHH130 pKa = 5.4MEE132 pKa = 3.76RR133 pKa = 11.84VAYY136 pKa = 9.8IPVRR140 pKa = 11.84HH141 pKa = 6.2EE142 pKa = 4.1NQGSKK147 pKa = 10.87CMVRR151 pKa = 11.84TRR153 pKa = 11.84LPYY156 pKa = 9.78YY157 pKa = 10.85GEE159 pKa = 4.36DD160 pKa = 3.4LEE162 pKa = 4.52EE163 pKa = 4.35QMFEE167 pKa = 4.13VLVSKK172 pKa = 8.75TSPLEE177 pKa = 4.18LIRR180 pKa = 11.84LASEE184 pKa = 4.17PLGSDD189 pKa = 3.35NQSEE193 pKa = 4.93PYY195 pKa = 10.18SSGYY199 pKa = 11.04DD200 pKa = 3.63EE201 pKa = 4.94VCVGAPAIEE210 pKa = 4.72CFLALPCPFEE220 pKa = 3.89QGTFNRR226 pKa = 11.84HH227 pKa = 5.25RR228 pKa = 11.84PSHH231 pKa = 6.7PLTDD235 pKa = 5.46EE236 pKa = 4.48IPSSGQNMILSDD248 pKa = 3.72ISDD251 pKa = 3.73LTSLLEE257 pKa = 4.37AMTISDD263 pKa = 3.65ASEE266 pKa = 4.25DD267 pKa = 3.52LSAGQNSFANTAQQVGGVGIEE288 pKa = 3.9VASAIEE294 pKa = 4.0TRR296 pKa = 11.84WYY298 pKa = 10.77SEE300 pKa = 3.57NAPWFEE306 pKa = 5.38EE307 pKa = 4.32IPQCSAFVGGFFAFVQEE324 pKa = 4.21EE325 pKa = 4.16PMDD328 pKa = 3.9FVSTFDD334 pKa = 3.9APLEE338 pKa = 3.98QMMTDD343 pKa = 3.31ADD345 pKa = 4.58EE346 pKa = 4.93EE347 pKa = 4.75DD348 pKa = 4.76FDD350 pKa = 6.25CSMSTISEE358 pKa = 4.44PEE360 pKa = 3.78TSEE363 pKa = 4.01MLDD366 pKa = 3.31VEE368 pKa = 4.25YY369 pKa = 11.09DD370 pKa = 3.97GIVVAGVSTACWYY383 pKa = 10.39NIHH386 pKa = 7.61DD387 pKa = 4.51EE388 pKa = 4.13LAMVPEE394 pKa = 4.62DD395 pKa = 3.65LCGDD399 pKa = 3.9SLMDD403 pKa = 3.95LAVEE407 pKa = 4.01VVPGIEE413 pKa = 5.15GILDD417 pKa = 4.13HH418 pKa = 7.05YY419 pKa = 11.29RR420 pKa = 11.84DD421 pKa = 4.21DD422 pKa = 5.13CLMYY426 pKa = 9.74WDD428 pKa = 4.95EE429 pKa = 4.37SLVPKK434 pKa = 10.68GSAIMMATMFPDD446 pKa = 3.18SGVAGSFPLDD456 pKa = 3.49QANMTNKK463 pKa = 10.18GATCVPQQVAEE474 pKa = 4.19DD475 pKa = 4.25LVWSPEE481 pKa = 3.78KK482 pKa = 10.42EE483 pKa = 3.96PRR485 pKa = 11.84VPSSPLLPGSDD496 pKa = 3.75SGADD500 pKa = 3.46LPGQSGVSLPEE511 pKa = 3.78VEE513 pKa = 5.19APEE516 pKa = 4.77PPTIPGVVVWLTTAPEE532 pKa = 4.32SPVKK536 pKa = 10.66VLEE539 pKa = 4.54EE540 pKa = 3.95EE541 pKa = 4.76DD542 pKa = 5.59KK543 pKa = 11.51EE544 pKa = 5.64DD545 pKa = 5.8DD546 pKa = 5.37DD547 pKa = 7.0DD548 pKa = 6.68DD549 pKa = 6.01EE550 pKa = 5.05EE551 pKa = 6.54DD552 pKa = 3.56SDD554 pKa = 5.24EE555 pKa = 4.95GYY557 pKa = 11.11EE558 pKa = 4.82DD559 pKa = 5.05DD560 pKa = 6.06DD561 pKa = 5.39EE562 pKa = 5.9GVTLQDD568 pKa = 3.4SALADD573 pKa = 3.77LLAEE577 pKa = 4.38LEE579 pKa = 4.32EE580 pKa = 5.15SDD582 pKa = 5.6FDD584 pKa = 5.35DD585 pKa = 4.39EE586 pKa = 4.67AA587 pKa = 6.32

Molecular weight: 63.75 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A162Y7C6|A0A162Y7C6_PHYB8 Uncharacterized protein OS=Phycomyces blakesleeanus (strain ATCC 8743b / DSM 1359 / FGSC 10004 / NBRC 33097 / NRRL 1555) OX=763407 GN=PHYBLDRAFT_140823 PE=4 SV=1
MM1 pKa = 8.02SMLRR5 pKa = 11.84QLWSSAVSRR14 pKa = 11.84LVPSTTNITQNTTISRR30 pKa = 11.84FTAARR35 pKa = 11.84PSMLGANTGSFLSSSFGLPRR55 pKa = 11.84SPFAASPFAATQMRR69 pKa = 11.84FVTYY73 pKa = 10.59GNTYY77 pKa = 9.82QPSNLVRR84 pKa = 11.84KK85 pKa = 9.15RR86 pKa = 11.84RR87 pKa = 11.84HH88 pKa = 5.25GFLSRR93 pKa = 11.84VATKK97 pKa = 10.31NGRR100 pKa = 11.84RR101 pKa = 11.84VLARR105 pKa = 11.84RR106 pKa = 11.84RR107 pKa = 11.84LKK109 pKa = 10.19GRR111 pKa = 11.84KK112 pKa = 8.68FLTHH116 pKa = 6.84

Molecular weight: 12.99 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

16154

1

16155

5761222

49

4633

356.6

40.25

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.434 ± 0.018

1.723 ± 0.009

5.426 ± 0.012

5.948 ± 0.017

4.253 ± 0.015

5.138 ± 0.018

2.701 ± 0.01

6.162 ± 0.015

6.085 ± 0.019

9.404 ± 0.021

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.463 ± 0.008

4.837 ± 0.013

4.905 ± 0.016

4.16 ± 0.015

5.125 ± 0.014

8.339 ± 0.02

6.132 ± 0.014

6.137 ± 0.016

1.245 ± 0.006

3.382 ± 0.01
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski