Anaerobium acetethylicum

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Clostridia; Eubacteriales; Lachnospiraceae; Anaerobium

Average proteome isoelectric point is 6.02

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4003 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1D3TYA2|A0A1D3TYA2_9FIRM HDIG domain-containing protein OS=Anaerobium acetethylicum OX=1619234 GN=SAMN05421730_10408 PE=4 SV=1
MM1 pKa = 7.93DD2 pKa = 6.07EE3 pKa = 4.29IYY5 pKa = 10.66QIIEE9 pKa = 3.59EE10 pKa = 4.73RR11 pKa = 11.84IRR13 pKa = 11.84QAGCMLDD20 pKa = 3.77IRR22 pKa = 11.84GCDD25 pKa = 3.03IYY27 pKa = 11.75AEE29 pKa = 4.1IADD32 pKa = 3.91FAEE35 pKa = 4.54DD36 pKa = 4.62KK37 pKa = 11.21EE38 pKa = 4.5DD39 pKa = 3.18GTYY42 pKa = 10.66IFMSKK47 pKa = 10.36PSDD50 pKa = 3.37NVVFEE55 pKa = 4.0YY56 pKa = 10.56KK57 pKa = 9.81IDD59 pKa = 3.71VMADD63 pKa = 3.41NLNLSYY69 pKa = 10.75IDD71 pKa = 3.49INSPEE76 pKa = 4.4GSCHH80 pKa = 7.21IDD82 pKa = 3.28FDD84 pKa = 4.16NN85 pKa = 4.59

Molecular weight: 9.8 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1D3TRS7|A0A1D3TRS7_9FIRM Uncharacterized protein OS=Anaerobium acetethylicum OX=1619234 GN=SAMN05421730_1004185 PE=4 SV=1
MM1 pKa = 7.67KK2 pKa = 8.72MTFQPKK8 pKa = 8.86KK9 pKa = 8.2RR10 pKa = 11.84SRR12 pKa = 11.84AKK14 pKa = 8.93VHH16 pKa = 5.83GFRR19 pKa = 11.84EE20 pKa = 4.24RR21 pKa = 11.84MSTAGGRR28 pKa = 11.84KK29 pKa = 8.71VLAARR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.1GRR39 pKa = 11.84KK40 pKa = 8.83KK41 pKa = 10.63LSAA44 pKa = 3.95

Molecular weight: 5.02 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4003

0

4003

1325591

24

2481

331.1

37.06

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

7.616 ± 0.043

1.342 ± 0.015

5.609 ± 0.03

7.676 ± 0.048

4.239 ± 0.029

7.255 ± 0.035

1.623 ± 0.016

7.888 ± 0.036

6.833 ± 0.034

8.637 ± 0.036

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

3.158 ± 0.019

4.645 ± 0.023

3.201 ± 0.023

2.895 ± 0.018

4.209 ± 0.029

6.069 ± 0.024

5.311 ± 0.035

6.727 ± 0.031

0.893 ± 0.015

4.173 ± 0.023
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski