Mycobacterium phage Panamaxus

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Fromanvirus; unclassified Fromanvirus

Average proteome isoelectric point is 5.99

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 83 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2P1N4W1|A0A2P1N4W1_9CAUD Uncharacterized protein OS=Mycobacterium phage Panamaxus OX=2126789 GN=35 PE=4 SV=1
MM1 pKa = 6.94THH3 pKa = 6.41VDD5 pKa = 3.61PLTSHH10 pKa = 6.71SEE12 pKa = 4.21PEE14 pKa = 4.06VDD16 pKa = 2.74WDD18 pKa = 4.41AIHH21 pKa = 7.6DD22 pKa = 4.06YY23 pKa = 10.84VYY25 pKa = 11.0EE26 pKa = 5.22GDD28 pKa = 4.14DD29 pKa = 3.46TNEE32 pKa = 3.7

Molecular weight: 3.72 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2P1N527|A0A2P1N527_9CAUD DNA-directed DNA polymerase OS=Mycobacterium phage Panamaxus OX=2126789 GN=42 PE=3 SV=1
MM1 pKa = 7.48PRR3 pKa = 11.84QRR5 pKa = 11.84RR6 pKa = 11.84APRR9 pKa = 11.84APLRR13 pKa = 11.84PRR15 pKa = 11.84LAHH18 pKa = 6.59PLGQRR23 pKa = 11.84RR24 pKa = 11.84PEE26 pKa = 3.91ALMSLLDD33 pKa = 3.53RR34 pKa = 11.84CEE36 pKa = 3.92QDD38 pKa = 3.41VVVYY42 pKa = 8.46PQEE45 pKa = 4.08VTTDD49 pKa = 3.15ADD51 pKa = 4.14GNTKK55 pKa = 8.61TRR57 pKa = 11.84PSKK60 pKa = 9.92TGFPAKK66 pKa = 10.43ARR68 pKa = 11.84IQVLGQSGTSSRR80 pKa = 11.84RR81 pKa = 11.84QEE83 pKa = 3.69QDD85 pKa = 2.78NEE87 pKa = 4.24GFEE90 pKa = 4.28SEE92 pKa = 4.3RR93 pKa = 11.84VYY95 pKa = 10.47MLHH98 pKa = 7.31FSRR101 pKa = 11.84KK102 pKa = 7.13FDD104 pKa = 3.73RR105 pKa = 11.84EE106 pKa = 4.07HH107 pKa = 6.72GVLGMQSQIEE117 pKa = 4.38WNGVRR122 pKa = 11.84WALFGEE128 pKa = 4.31PAFYY132 pKa = 10.07TGSRR136 pKa = 11.84RR137 pKa = 11.84TQHH140 pKa = 5.99IGYY143 pKa = 8.01TMKK146 pKa = 10.6RR147 pKa = 11.84YY148 pKa = 9.51

Molecular weight: 17.15 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

83

0

83

15454

26

1012

186.2

20.6

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.784 ± 0.395

0.848 ± 0.132

6.568 ± 0.253

6.95 ± 0.351

3.391 ± 0.201

8.703 ± 0.567

1.974 ± 0.173

4.743 ± 0.168

4.187 ± 0.208

8.043 ± 0.218

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.42 ± 0.156

3.332 ± 0.218

5.701 ± 0.34

3.889 ± 0.283

6.665 ± 0.378

4.924 ± 0.192

5.785 ± 0.234

7.137 ± 0.163

1.987 ± 0.148

2.97 ± 0.2
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski