Circovirus-like genome BBC-A

Taxonomy: Viruses; unclassified viruses; unclassified DNA viruses; unclassified ssDNA viruses

Average proteome isoelectric point is 7.5

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|C6GIJ5|C6GIJ5_9VIRU Uncharacterized protein OS=Circovirus-like genome BBC-A OX=642260 PE=4 SV=1
MM1 pKa = 7.46CFSNISHH8 pKa = 7.17INTKK12 pKa = 9.12CHH14 pKa = 7.01HH15 pKa = 6.79EE16 pKa = 4.06NTQKK20 pKa = 11.26KK21 pKa = 9.34NFTKK25 pKa = 10.43KK26 pKa = 8.22VQNVVRR32 pKa = 11.84RR33 pKa = 11.84MAEE36 pKa = 3.99TKK38 pKa = 10.23IQDD41 pKa = 3.32YY42 pKa = 10.77SINEE46 pKa = 4.15DD47 pKa = 3.56VLISNFDD54 pKa = 3.36TPYY57 pKa = 10.87VQDD60 pKa = 3.53LTQIEE65 pKa = 4.31RR66 pKa = 11.84GGASNQRR73 pKa = 11.84IGNEE77 pKa = 3.4ILMSGVKK84 pKa = 8.72WRR86 pKa = 11.84LLFHH90 pKa = 6.87CRR92 pKa = 11.84NNPNYY97 pKa = 9.21EE98 pKa = 4.01DD99 pKa = 3.64VVIRR103 pKa = 11.84SLWCRR108 pKa = 11.84IAIIEE113 pKa = 4.23MKK115 pKa = 9.67NTGTTDD121 pKa = 3.2VVLLEE126 pKa = 4.12DD127 pKa = 4.78FFRR130 pKa = 11.84KK131 pKa = 9.24GANSMDD137 pKa = 3.45FDD139 pKa = 4.19AVTEE143 pKa = 4.08AEE145 pKa = 4.28RR146 pKa = 11.84YY147 pKa = 8.26YY148 pKa = 11.34LPVDD152 pKa = 4.01LTNKK156 pKa = 7.9KK157 pKa = 7.6TLYY160 pKa = 8.88QTTFKK165 pKa = 10.52IGVKK169 pKa = 10.0NQVDD173 pKa = 3.63TADD176 pKa = 3.68YY177 pKa = 10.61LSNKK181 pKa = 9.01IIKK184 pKa = 9.88NYY186 pKa = 10.04RR187 pKa = 11.84KK188 pKa = 9.53VSRR191 pKa = 11.84SINFEE196 pKa = 3.68SSDD199 pKa = 3.33ISNSASTKK207 pKa = 10.42LYY209 pKa = 8.38FVCWIGNNDD218 pKa = 3.46LDD220 pKa = 5.19ANNATTNNAGACEE233 pKa = 3.84LSGLLSTYY241 pKa = 10.95YY242 pKa = 10.85KK243 pKa = 10.92DD244 pKa = 3.59FF245 pKa = 4.86

Molecular weight: 28.13 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|C6GIJ5|C6GIJ5_9VIRU Uncharacterized protein OS=Circovirus-like genome BBC-A OX=642260 PE=4 SV=1
MM1 pKa = 7.85KK2 pKa = 9.41ITTEE6 pKa = 3.73PTKK9 pKa = 10.77RR10 pKa = 11.84RR11 pKa = 11.84DD12 pKa = 3.02WAFTDD17 pKa = 4.87FNMEE21 pKa = 3.89NQTKK25 pKa = 9.32YY26 pKa = 11.2ASLVEE31 pKa = 4.08QCVCSYY37 pKa = 10.56VCFGIEE43 pKa = 3.99YY44 pKa = 9.91CPKK47 pKa = 9.43TNRR50 pKa = 11.84PHH52 pKa = 5.77LQGYY56 pKa = 7.5LHH58 pKa = 6.97FKK60 pKa = 10.15NPRR63 pKa = 11.84TTRR66 pKa = 11.84EE67 pKa = 3.64GRR69 pKa = 11.84NVFGKK74 pKa = 7.55THH76 pKa = 7.19SEE78 pKa = 4.37FCWHH82 pKa = 6.22SRR84 pKa = 11.84EE85 pKa = 4.98KK86 pKa = 10.88NINYY90 pKa = 8.48CKK92 pKa = 10.49KK93 pKa = 7.82GTKK96 pKa = 9.94VLGEE100 pKa = 4.12TFFEE104 pKa = 4.69FGKK107 pKa = 10.66KK108 pKa = 9.22PNQGYY113 pKa = 7.75RR114 pKa = 11.84TDD116 pKa = 3.69IEE118 pKa = 4.13NVEE121 pKa = 4.59YY122 pKa = 10.33ILKK125 pKa = 5.97TTRR128 pKa = 11.84SIQGIIDD135 pKa = 3.35NHH137 pKa = 6.0FNLYY141 pKa = 8.21IQYY144 pKa = 10.46RR145 pKa = 11.84SRR147 pKa = 11.84FLNYY151 pKa = 10.47LEE153 pKa = 4.79DD154 pKa = 3.96TEE156 pKa = 4.49KK157 pKa = 10.77PRR159 pKa = 11.84AEE161 pKa = 3.93MTKK164 pKa = 10.28GIWYY168 pKa = 8.64YY169 pKa = 11.2GPPGCGKK176 pKa = 8.89STVIKK181 pKa = 10.78NNFFGTEE188 pKa = 4.45VVWLEE193 pKa = 3.54YY194 pKa = 10.33DD195 pKa = 3.45GKK197 pKa = 11.18YY198 pKa = 10.5FSDD201 pKa = 4.27YY202 pKa = 10.87NGHH205 pKa = 4.89TTVIFDD211 pKa = 4.48DD212 pKa = 3.63QDD214 pKa = 2.83ISKK217 pKa = 10.15FSRR220 pKa = 11.84SMILKK225 pKa = 9.83LINHH229 pKa = 6.39TPFKK233 pKa = 10.49LRR235 pKa = 11.84CLGSYY240 pKa = 10.4RR241 pKa = 11.84EE242 pKa = 4.19FNAITVIFIDD252 pKa = 3.52NFHH255 pKa = 6.36YY256 pKa = 10.43SHH258 pKa = 6.98YY259 pKa = 10.25FGEE262 pKa = 4.91DD263 pKa = 2.75EE264 pKa = 5.27AIARR268 pKa = 11.84RR269 pKa = 11.84LKK271 pKa = 10.53AIQFGTEE278 pKa = 3.86VPKK281 pKa = 11.04

Molecular weight: 33.21 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2

0

2

526

245

281

263.0

30.67

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

3.612 ± 0.865

2.662 ± 0.143

5.323 ± 0.813

6.084 ± 0.798

6.274 ± 1.2

5.513 ± 0.963

2.471 ± 0.564

7.224 ± 0.083

7.795 ± 0.301

5.894 ± 0.703

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.711 ± 0.222

8.365 ± 1.512

2.471 ± 0.839

3.042 ± 0.15

5.513 ± 0.414

5.894 ± 0.978

7.985 ± 0.155

5.323 ± 0.813

1.331 ± 0.072

5.513 ± 0.689
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski