Klebsiella phage KPV811

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Slopekvirinae; Drulisvirus; Klebsiella virus KPV811

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 43 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1J0MID7|A0A1J0MID7_9CAUD DNA primase/helicase OS=Klebsiella phage KPV811 OX=1913574 PE=4 SV=1
MM1 pKa = 7.94DD2 pKa = 5.01KK3 pKa = 11.13VLDD6 pKa = 4.29AYY8 pKa = 10.19KK9 pKa = 10.84NLAKK13 pKa = 10.42AVASVVYY20 pKa = 10.13DD21 pKa = 3.49SAHH24 pKa = 7.09DD25 pKa = 3.92YY26 pKa = 11.31VVDD29 pKa = 4.32DD30 pKa = 5.26DD31 pKa = 4.94EE32 pKa = 7.35LGTLYY37 pKa = 10.91DD38 pKa = 4.68ALDD41 pKa = 3.96KK42 pKa = 11.11LAALYY47 pKa = 10.99GMDD50 pKa = 4.95LEE52 pKa = 4.79LAATAFKK59 pKa = 10.44EE60 pKa = 4.24HH61 pKa = 6.94NDD63 pKa = 3.63LAAHH67 pKa = 6.58ADD69 pKa = 3.59KK70 pKa = 11.41LKK72 pKa = 11.01GDD74 pKa = 4.2DD75 pKa = 3.72LAVIRR80 pKa = 11.84IVGTLSIGLAEE91 pKa = 4.81IGSCIYY97 pKa = 10.87DD98 pKa = 3.05VDD100 pKa = 3.62QSLRR104 pKa = 11.84TPEE107 pKa = 4.5VIGDD111 pKa = 3.64MLGTVLVLSEE121 pKa = 5.16LEE123 pKa = 4.26SCC125 pKa = 5.24

Molecular weight: 13.41 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1J0MHZ5|A0A1J0MHZ5_9CAUD Tail fibers protein OS=Klebsiella phage KPV811 OX=1913574 PE=4 SV=1
MM1 pKa = 7.1YY2 pKa = 10.08GKK4 pKa = 9.94NPEE7 pKa = 3.98VLLMRR12 pKa = 11.84KK13 pKa = 7.4QQPTIDD19 pKa = 3.62GLARR23 pKa = 11.84EE24 pKa = 4.55YY25 pKa = 10.64SAKK28 pKa = 10.43AALRR32 pKa = 11.84QHH34 pKa = 6.15YY35 pKa = 7.59EE36 pKa = 3.85KK37 pKa = 10.33QAKK40 pKa = 8.74RR41 pKa = 11.84LGMTLRR47 pKa = 11.84GYY49 pKa = 7.79CHH51 pKa = 7.01RR52 pKa = 11.84FNVRR56 pKa = 11.84GVVV59 pKa = 3.11

Molecular weight: 6.88 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

43

0

43

12239

37

1232

284.6

31.47

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.646 ± 0.564

1.103 ± 0.222

6.496 ± 0.283

5.866 ± 0.393

3.007 ± 0.16

7.239 ± 0.229

1.749 ± 0.141

4.608 ± 0.147

4.862 ± 0.306

8.824 ± 0.308

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.909 ± 0.223

4.224 ± 0.296

3.93 ± 0.281

4.943 ± 0.42

5.654 ± 0.288

6.259 ± 0.276

5.777 ± 0.303

6.684 ± 0.213

1.34 ± 0.099

3.881 ± 0.303
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski