Streptococcus pantholopis
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1974 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A172Q7Y7|A0A172Q7Y7_9STRE Uncharacterized protein OS=Streptococcus pantholopis OX=1811193 GN=A0O21_05815 PE=4 SV=1
MM1 pKa = 7.44 ARR3 pKa = 11.84 HH4 pKa = 6.16 RR5 pKa = 11.84 KK6 pKa = 9.13 KK7 pKa = 10.82 ADD9 pKa = 3.1 NSNTLRR15 pKa = 11.84 IFLILGVVLLALGLIIFAVSSYY37 pKa = 10.76 INNRR41 pKa = 11.84 EE42 pKa = 3.94 SSTTTSSSTAQTLMSSSDD60 pKa = 3.16 TSEE63 pKa = 3.9 NTEE66 pKa = 4.01 TSQTDD71 pKa = 3.31 QALDD75 pKa = 3.18 IAALANEE82 pKa = 5.31 DD83 pKa = 3.94 YY84 pKa = 11.29 NSAAGVWLSSNGNSLTFNAEE104 pKa = 3.92 GLEE107 pKa = 4.11 SSDD110 pKa = 3.06 IQGTIDD116 pKa = 3.72 FTITPYY122 pKa = 10.51 QQLDD126 pKa = 3.86 DD127 pKa = 3.95 NTYY130 pKa = 10.87 LATLTSADD138 pKa = 3.23 GSLVYY143 pKa = 10.48 DD144 pKa = 4.44 LYY146 pKa = 11.06 FIKK149 pKa = 10.54 AGSEE153 pKa = 3.91 LPEE156 pKa = 5.09 DD157 pKa = 4.25 YY158 pKa = 10.36 FAEE161 pKa = 5.75 GYY163 pKa = 9.12 TDD165 pKa = 3.76 TSDD168 pKa = 3.24 HH169 pKa = 7.04 DD170 pKa = 4.06 KK171 pKa = 11.3 DD172 pKa = 3.5 RR173 pKa = 11.84 LYY175 pKa = 9.82 ITSDD179 pKa = 3.21 LVGGEE184 pKa = 4.71 DD185 pKa = 3.69 YY186 pKa = 11.4 ADD188 pKa = 4.07 NILYY192 pKa = 9.9 RR193 pKa = 11.84 QQ194 pKa = 3.42
Molecular weight: 21.2 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.746
IPC2_protein 3.897
IPC_protein 3.897
Toseland 3.668
ProMoST 4.062
Dawson 3.884
Bjellqvist 4.037
Wikipedia 3.834
Rodwell 3.719
Grimsley 3.579
Solomon 3.884
Lehninger 3.834
Nozaki 3.999
DTASelect 4.253
Thurlkill 3.732
EMBOSS 3.834
Sillero 4.012
Patrickios 1.914
IPC_peptide 3.872
IPC2_peptide 3.986
IPC2.peptide.svr19 3.907
Protein with the highest isoelectric point:
>tr|A0A172Q571|A0A172Q571_9STRE Argininosuccinate lyase OS=Streptococcus pantholopis OX=1811193 GN=argH PE=3 SV=1
MM1 pKa = 7.35 KK2 pKa = 9.43 RR3 pKa = 11.84 TYY5 pKa = 10.07 QPSKK9 pKa = 9.67 IRR11 pKa = 11.84 RR12 pKa = 11.84 QRR14 pKa = 11.84 KK15 pKa = 7.52 HH16 pKa = 5.18 GFRR19 pKa = 11.84 HH20 pKa = 6.46 RR21 pKa = 11.84 MATKK25 pKa = 10.2 NGRR28 pKa = 11.84 HH29 pKa = 4.81 VLASRR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 KK37 pKa = 8.73 GRR39 pKa = 11.84 KK40 pKa = 8.76 VLSAA44 pKa = 4.05
Molecular weight: 5.34 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.433
IPC2_protein 11.125
IPC_protein 12.501
Toseland 12.661
ProMoST 13.159
Dawson 12.661
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.384
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.106
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.067
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1974
0
1974
602372
43
3130
305.2
34.08
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
8.458 ± 0.066
0.559 ± 0.015
5.764 ± 0.045
6.592 ± 0.056
4.58 ± 0.041
6.552 ± 0.054
1.852 ± 0.028
7.112 ± 0.052
6.363 ± 0.051
10.375 ± 0.075
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.342 ± 0.024
4.241 ± 0.04
3.32 ± 0.036
4.225 ± 0.038
4.185 ± 0.038
6.489 ± 0.052
5.616 ± 0.06
6.618 ± 0.045
0.889 ± 0.021
3.865 ± 0.04
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here