Sulfitobacter geojensis
Average proteome isoelectric point is 6.07
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3982 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A196Q3N3|A0A196Q3N3_9RHOB Homogentisate 1 2-dioxygenase OS=Sulfitobacter geojensis OX=1342299 GN=A8B74_04715 PE=3 SV=1
MM1 pKa = 7.29 KK2 pKa = 10.49 RR3 pKa = 11.84 LVTTTALTLIAGAGTAFAGSLAEE26 pKa = 4.12 PVVTPPVLTPQVASADD42 pKa = 3.37 WTGAYY47 pKa = 10.11 AGIQLGYY54 pKa = 11.23 GNLNVANQIDD64 pKa = 4.2 RR65 pKa = 11.84 DD66 pKa = 3.69 GDD68 pKa = 3.78 GDD70 pKa = 3.9 VDD72 pKa = 4.74 GDD74 pKa = 4.23 DD75 pKa = 3.35 EE76 pKa = 4.54 TFAFGGGLDD85 pKa = 3.76 GNGAIGGAHH94 pKa = 7.43 LGYY97 pKa = 9.36 MWDD100 pKa = 3.45 FGNYY104 pKa = 7.08 VAGAEE109 pKa = 4.05 LDD111 pKa = 3.92 YY112 pKa = 11.38 NAANIDD118 pKa = 3.52 IGSVGEE124 pKa = 4.04 LDD126 pKa = 3.47 SVARR130 pKa = 11.84 AKK132 pKa = 10.81 LKK134 pKa = 10.61 LGYY137 pKa = 10.87 DD138 pKa = 3.28 MGDD141 pKa = 3.27 TLVYY145 pKa = 10.44 GVLGAAKK152 pKa = 10.15 ADD154 pKa = 3.6 ATIGGNDD161 pKa = 3.52 FSEE164 pKa = 4.27 NGYY167 pKa = 9.7 VAGFGVDD174 pKa = 3.5 YY175 pKa = 10.63 LVSEE179 pKa = 4.26 NVVLGGEE186 pKa = 4.29 VLYY189 pKa = 10.88 HH190 pKa = 7.35 DD191 pKa = 5.38 FGDD194 pKa = 5.0 DD195 pKa = 4.25 FDD197 pKa = 6.08 GLDD200 pKa = 3.9 LSADD204 pKa = 3.33 ATTVQVKK211 pKa = 9.24 MSYY214 pKa = 10.72 KK215 pKa = 10.11 FF216 pKa = 3.27
Molecular weight: 22.22 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.68
IPC2_protein 3.694
IPC_protein 3.719
Toseland 3.478
ProMoST 3.91
Dawson 3.732
Bjellqvist 3.884
Wikipedia 3.706
Rodwell 3.541
Grimsley 3.389
Solomon 3.719
Lehninger 3.681
Nozaki 3.846
DTASelect 4.139
Thurlkill 3.554
EMBOSS 3.706
Sillero 3.846
Patrickios 1.074
IPC_peptide 3.719
IPC2_peptide 3.821
IPC2.peptide.svr19 3.762
Protein with the highest isoelectric point:
>tr|A0A196Q1A2|A0A196Q1A2_9RHOB 50S ribosomal protein L15 OS=Sulfitobacter geojensis OX=1342299 GN=rplO PE=3 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.61 RR3 pKa = 11.84 TFQPSNLVRR12 pKa = 11.84 KK13 pKa = 9.18 RR14 pKa = 11.84 RR15 pKa = 11.84 HH16 pKa = 4.42 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.37 AGRR28 pKa = 11.84 KK29 pKa = 8.81 IINARR34 pKa = 11.84 RR35 pKa = 11.84 AQGRR39 pKa = 11.84 SKK41 pKa = 11.13 LSAA44 pKa = 3.7
Molecular weight: 5.11 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.517
IPC2_protein 11.199
IPC_protein 12.793
Toseland 12.969
ProMoST 13.451
Dawson 12.969
Bjellqvist 12.954
Wikipedia 13.437
Rodwell 12.647
Grimsley 12.998
Solomon 13.451
Lehninger 13.364
Nozaki 12.954
DTASelect 12.954
Thurlkill 12.954
EMBOSS 13.466
Sillero 12.954
Patrickios 12.369
IPC_peptide 13.466
IPC2_peptide 12.442
IPC2.peptide.svr19 9.154
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3982
0
3982
1277318
41
2149
320.8
34.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.166 ± 0.052
0.891 ± 0.011
6.236 ± 0.037
5.634 ± 0.038
3.814 ± 0.023
8.538 ± 0.038
2.034 ± 0.019
5.339 ± 0.03
3.594 ± 0.035
9.807 ± 0.038
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.952 ± 0.02
2.848 ± 0.021
4.845 ± 0.032
3.462 ± 0.024
6.041 ± 0.033
5.27 ± 0.028
5.683 ± 0.027
7.296 ± 0.031
1.332 ± 0.017
2.217 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here