bacterium HR40

Taxonomy: cellular organisms; Bacteria; unclassified Bacteria

Average proteome isoelectric point is 6.92

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2654 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A2H6AZU8|A0A2H6AZU8_9BACT COQ9 domain-containing protein OS=bacterium HR40 OX=2035435 GN=HRbin40_00915 PE=4 SV=1
MM1 pKa = 7.61TEE3 pKa = 4.23TEE5 pKa = 4.22TEE7 pKa = 3.76AAAAGAAGQSSAGAWEE23 pKa = 4.11PAEE26 pKa = 4.08KK27 pKa = 10.18ADD29 pKa = 4.55RR30 pKa = 11.84VVLNLSEE37 pKa = 4.56LLCDD41 pKa = 3.78EE42 pKa = 4.54NGEE45 pKa = 4.32VVLYY49 pKa = 10.36NDD51 pKa = 3.0SGFRR55 pKa = 11.84RR56 pKa = 11.84VVLEE60 pKa = 3.61ADD62 pKa = 3.63ARR64 pKa = 11.84PVAQGMAEE72 pKa = 3.98PHH74 pKa = 5.47LTASGEE80 pKa = 4.21QVAGYY85 pKa = 10.69AYY87 pKa = 10.5VAFDD91 pKa = 3.44NGMTLYY97 pKa = 10.58YY98 pKa = 10.16PPDD101 pKa = 4.01LEE103 pKa = 5.46LIVVPGPAA111 pKa = 3.64

Molecular weight: 11.66 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A2H6B384|A0A2H6B384_9BACT Diguanylate cyclase VdcA OS=bacterium HR40 OX=2035435 GN=vdcA_2 PE=4 SV=1
MM1 pKa = 7.99PSTTSSSVSRR11 pKa = 11.84DD12 pKa = 3.4FASSTVITPSLPTFSIAWASMRR34 pKa = 11.84PISRR38 pKa = 11.84SPLAEE43 pKa = 4.03MVPIWAISSLVVTVLARR60 pKa = 11.84FRR62 pKa = 11.84SSCTTASTAISMPRR76 pKa = 11.84RR77 pKa = 11.84RR78 pKa = 11.84SMALAPASTAFTPSRR93 pKa = 11.84AMAAASTVAVVVPSPATSLVRR114 pKa = 11.84FATSFTIWTPRR125 pKa = 11.84FSNLSSSSISLATVTPSLVMRR146 pKa = 11.84GAPKK150 pKa = 10.46LFSSTTLRR158 pKa = 11.84PRR160 pKa = 11.84GPRR163 pKa = 11.84VTAVARR169 pKa = 11.84ARR171 pKa = 11.84VSTPRR176 pKa = 11.84RR177 pKa = 11.84IFSRR181 pKa = 11.84ASVEE185 pKa = 3.83KK186 pKa = 9.99RR187 pKa = 11.84ISFAVMLLPPAGTKK201 pKa = 8.68RR202 pKa = 11.84TAARR206 pKa = 11.84SVGTRR211 pKa = 11.84RR212 pKa = 11.84VGVGNRR218 pKa = 11.84SALAQDD224 pKa = 3.58GGRR227 pKa = 11.84RR228 pKa = 11.84VRR230 pKa = 11.84SRR232 pKa = 11.84WRR234 pKa = 11.84TPWW237 pKa = 3.3

Molecular weight: 25.34 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2654

0

2654

892615

29

1694

336.3

36.73

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

13.311 ± 0.066

0.992 ± 0.014

5.062 ± 0.035

6.842 ± 0.047

3.615 ± 0.028

8.523 ± 0.039

2.075 ± 0.02

4.015 ± 0.033

1.698 ± 0.034

11.994 ± 0.065

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.826 ± 0.019

1.575 ± 0.017

6.066 ± 0.036

2.5 ± 0.022

10.061 ± 0.048

4.23 ± 0.026

4.298 ± 0.028

8.015 ± 0.035

1.426 ± 0.019

1.874 ± 0.024
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski