Amino acid dipepetide frequency for bacterium HR40

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	19.484AlaAla: 19.484 ± 0.223	1.461AlaCys: 1.461 ± 0.043	6.388AlaAsp: 6.388 ± 0.097	9.507AlaGlu: 9.507 ± 0.132	4.682AlaPhe: 4.682 ± 0.077	11.408AlaGly: 11.408 ± 0.134	2.489AlaHis: 2.489 ± 0.054	6.041AlaIle: 6.041 ± 0.101	2.248AlaLys: 2.248 ± 0.053	15.549AlaLeu: 15.549 ± 0.184	2.886AlaMet: 2.886 ± 0.056	1.986AlaAsn: 1.986 ± 0.051	5.917AlaPro: 5.917 ± 0.087	3.12AlaGln: 3.12 ± 0.057	13.834AlaArg: 13.834 ± 0.162	5.887AlaSer: 5.887 ± 0.08	6.094AlaThr: 6.094 ± 0.084	10.24AlaVal: 10.24 ± 0.118	1.773AlaTrp: 1.773 ± 0.05	2.115AlaTyr: 2.115 ± 0.053	0.0AlaXaa: 0.0 ± 0.0
Cys	1.195CysAla: 1.195 ± 0.04	0.136CysCys: 0.136 ± 0.012	0.586CysAsp: 0.586 ± 0.027	0.599CysGlu: 0.599 ± 0.029	0.35CysPhe: 0.35 ± 0.02	1.207CysGly: 1.207 ± 0.039	0.298CysHis: 0.298 ± 0.019	0.366CysIle: 0.366 ± 0.021	0.096CysLys: 0.096 ± 0.011	0.961CysLeu: 0.961 ± 0.035	0.14CysMet: 0.14 ± 0.011	0.176CysAsn: 0.176 ± 0.015	0.614CysPro: 0.614 ± 0.03	0.203CysGln: 0.203 ± 0.014	1.152CysArg: 1.152 ± 0.041	0.43CysSer: 0.43 ± 0.022	0.428CysThr: 0.428 ± 0.021	0.632CysVal: 0.632 ± 0.026	0.141CysTrp: 0.141 ± 0.013	0.214CysTyr: 0.214 ± 0.015	0.0CysXaa: 0.0 ± 0.0
Asp	5.468AspAla: 5.468 ± 0.087	0.463AspCys: 0.463 ± 0.025	2.287AspAsp: 2.287 ± 0.059	3.17AspGlu: 3.17 ± 0.066	1.836AspPhe: 1.836 ± 0.047	4.778AspGly: 4.778 ± 0.099	1.102AspHis: 1.102 ± 0.034	2.282AspIle: 2.282 ± 0.056	0.697AspLys: 0.697 ± 0.029	6.223AspLeu: 6.223 ± 0.088	0.868AspMet: 0.868 ± 0.032	0.767AspAsn: 0.767 ± 0.029	4.78AspPro: 4.78 ± 0.082	1.053AspGln: 1.053 ± 0.037	5.665AspArg: 5.665 ± 0.078	1.794AspSer: 1.794 ± 0.051	1.906AspThr: 1.906 ± 0.049	3.47AspVal: 3.47 ± 0.07	0.886AspTrp: 0.886 ± 0.032	1.126AspTyr: 1.126 ± 0.033	0.0AspXaa: 0.0 ± 0.0
Glu	9.366GluAla: 9.366 ± 0.133	0.384GluCys: 0.384 ± 0.02	2.912GluAsp: 2.912 ± 0.057	4.944GluGlu: 4.944 ± 0.099	1.609GluPhe: 1.609 ± 0.044	5.14GluGly: 5.14 ± 0.085	1.229GluHis: 1.229 ± 0.035	3.401GluIle: 3.401 ± 0.068	1.558GluLys: 1.558 ± 0.049	8.468GluLeu: 8.468 ± 0.117	1.272GluMet: 1.272 ± 0.036	1.054GluAsn: 1.054 ± 0.034	4.077GluPro: 4.077 ± 0.067	2.028GluGln: 2.028 ± 0.053	8.741GluArg: 8.741 ± 0.128	2.26GluSer: 2.26 ± 0.052	3.01GluThr: 3.01 ± 0.057	5.358GluVal: 5.358 ± 0.082	0.794GluTrp: 0.794 ± 0.032	0.814GluTyr: 0.814 ± 0.034	0.0GluXaa: 0.0 ± 0.0
Phe	5.67PheAla: 5.67 ± 0.084	0.437PheCys: 0.437 ± 0.023	2.502PheAsp: 2.502 ± 0.056	2.523PheGlu: 2.523 ± 0.062	1.314PhePhe: 1.314 ± 0.044	3.631PheGly: 3.631 ± 0.063	0.745PheHis: 0.745 ± 0.029	0.849PheIle: 0.849 ± 0.034	0.445PheLys: 0.445 ± 0.025	3.853PheLeu: 3.853 ± 0.092	0.476PheMet: 0.476 ± 0.024	0.555PheAsn: 0.555 ± 0.026	1.786PhePro: 1.786 ± 0.046	0.701PheGln: 0.701 ± 0.03	3.015PheArg: 3.015 ± 0.063	1.434PheSer: 1.434 ± 0.043	1.586PheThr: 1.586 ± 0.044	3.341PheVal: 3.341 ± 0.055	0.533PheTrp: 0.533 ± 0.025	0.752PheTyr: 0.752 ± 0.029	0.0PheXaa: 0.0 ± 0.0
Gly	8.597GlyAla: 8.597 ± 0.098	1.11GlyCys: 1.11 ± 0.038	3.883GlyAsp: 3.883 ± 0.074	6.174GlyGlu: 6.174 ± 0.087	3.55GlyPhe: 3.55 ± 0.073	7.047GlyGly: 7.047 ± 0.111	1.998GlyHis: 1.998 ± 0.05	4.346GlyIle: 4.346 ± 0.074	1.931GlyLys: 1.931 ± 0.052	9.954GlyLeu: 9.954 ± 0.119	2.018GlyMet: 2.018 ± 0.049	1.372GlyAsn: 1.372 ± 0.042	4.225GlyPro: 4.225 ± 0.07	2.098GlyGln: 2.098 ± 0.049	9.281GlyArg: 9.281 ± 0.118	4.206GlySer: 4.206 ± 0.067	4.135GlyThr: 4.135 ± 0.074	5.952GlyVal: 5.952 ± 0.094	1.394GlyTrp: 1.394 ± 0.041	1.965GlyTyr: 1.965 ± 0.052	0.0GlyXaa: 0.0 ± 0.0
His	2.443HisAla: 2.443 ± 0.055	0.249HisCys: 0.249 ± 0.017	1.002HisAsp: 1.002 ± 0.032	1.191HisGlu: 1.191 ± 0.036	0.841HisPhe: 0.841 ± 0.032	2.093HisGly: 2.093 ± 0.052	0.509HisHis: 0.509 ± 0.025	0.788HisIle: 0.788 ± 0.029	0.248HisLys: 0.248 ± 0.017	2.625HisLeu: 2.625 ± 0.055	0.373HisMet: 0.373 ± 0.022	0.366HisAsn: 0.366 ± 0.022	1.833HisPro: 1.833 ± 0.043	0.418HisGln: 0.418 ± 0.023	2.086HisArg: 2.086 ± 0.051	0.739HisSer: 0.739 ± 0.029	0.748HisThr: 0.748 ± 0.033	1.368HisVal: 1.368 ± 0.034	0.342HisTrp: 0.342 ± 0.021	0.493HisTyr: 0.493 ± 0.023	0.0HisXaa: 0.0 ± 0.0
Ile	6.25IleAla: 6.25 ± 0.091	0.443IleCys: 0.443 ± 0.023	2.88IleAsp: 2.88 ± 0.056	3.323IleGlu: 3.323 ± 0.062	1.241IlePhe: 1.241 ± 0.036	3.95IleGly: 3.95 ± 0.066	0.777IleHis: 0.777 ± 0.029	0.807IleIle: 0.807 ± 0.036	0.543IleLys: 0.543 ± 0.024	4.28IleLeu: 4.28 ± 0.082	0.444IleMet: 0.444 ± 0.023	0.714IleAsn: 0.714 ± 0.033	2.054IlePro: 2.054 ± 0.048	0.747IleGln: 0.747 ± 0.033	3.512IleArg: 3.512 ± 0.058	1.427IleSer: 1.427 ± 0.042	1.489IleThr: 1.489 ± 0.039	3.975IleVal: 3.975 ± 0.081	0.509IleTrp: 0.509 ± 0.025	0.79IleTyr: 0.79 ± 0.035	0.0IleXaa: 0.0 ± 0.0
Lys	1.983LysAla: 1.983 ± 0.05	0.1LysCys: 0.1 ± 0.011	0.767LysAsp: 0.767 ± 0.032	0.98LysGlu: 0.98 ± 0.041	0.473LysPhe: 0.473 ± 0.023	1.423LysGly: 1.423 ± 0.046	0.28LysHis: 0.28 ± 0.02	0.883LysIle: 0.883 ± 0.03	0.564LysLys: 0.564 ± 0.03	1.943LysLeu: 1.943 ± 0.054	0.302LysMet: 0.302 ± 0.021	0.347LysAsn: 0.347 ± 0.021	1.176LysPro: 1.176 ± 0.04	0.438LysGln: 0.438 ± 0.027	1.748LysArg: 1.748 ± 0.053	0.818LysSer: 0.818 ± 0.031	0.832LysThr: 0.832 ± 0.031	1.313LysVal: 1.313 ± 0.045	0.211LysTrp: 0.211 ± 0.015	0.399LysTyr: 0.399 ± 0.026	0.0LysXaa: 0.0 ± 0.0
Leu	20.698LeuAla: 20.698 ± 0.249	1.21LeuCys: 1.21 ± 0.036	6.496LeuAsp: 6.496 ± 0.11	8.394LeuGlu: 8.394 ± 0.114	4.477LeuPhe: 4.477 ± 0.082	9.685LeuGly: 9.685 ± 0.121	2.195LeuHis: 2.195 ± 0.058	2.81LeuIle: 2.81 ± 0.063	1.599LeuLys: 1.599 ± 0.052	14.239LeuLeu: 14.239 ± 0.198	1.503LeuMet: 1.503 ± 0.042	1.31LeuAsn: 1.31 ± 0.04	7.478LeuPro: 7.478 ± 0.102	2.633LeuGln: 2.633 ± 0.06	10.726LeuArg: 10.726 ± 0.14	5.224LeuSer: 5.224 ± 0.075	4.224LeuThr: 4.224 ± 0.069	11.236LeuVal: 11.236 ± 0.138	1.763LeuTrp: 1.763 ± 0.054	2.043LeuTyr: 2.043 ± 0.049	0.0LeuXaa: 0.0 ± 0.0
Met	2.514MetAla: 2.514 ± 0.055	0.145MetCys: 0.145 ± 0.012	0.697MetAsp: 0.697 ± 0.028	0.877MetGlu: 0.877 ± 0.036	0.548MetPhe: 0.548 ± 0.023	1.417MetGly: 1.417 ± 0.041	0.32MetHis: 0.32 ± 0.018	0.745MetIle: 0.745 ± 0.032	0.408MetLys: 0.408 ± 0.021	2.032MetLeu: 2.032 ± 0.051	0.344MetMet: 0.344 ± 0.023	0.406MetAsn: 0.406 ± 0.022	1.219MetPro: 1.219 ± 0.04	0.504MetGln: 0.504 ± 0.026	1.955MetArg: 1.955 ± 0.045	1.117MetSer: 1.117 ± 0.037	1.164MetThr: 1.164 ± 0.035	1.406MetVal: 1.406 ± 0.039	0.194MetTrp: 0.194 ± 0.013	0.252MetTyr: 0.252 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	1.849AsnAla: 1.849 ± 0.043	0.169AsnCys: 0.169 ± 0.014	0.781AsnAsp: 0.781 ± 0.039	0.771AsnGlu: 0.771 ± 0.032	0.579AsnPhe: 0.579 ± 0.028	1.462AsnGly: 1.462 ± 0.04	0.298AsnHis: 0.298 ± 0.018	0.747AsnIle: 0.747 ± 0.032	0.226AsnLys: 0.226 ± 0.017	1.909AsnLeu: 1.909 ± 0.044	0.313AsnMet: 0.313 ± 0.019	0.352AsnAsn: 0.352 ± 0.019	1.292AsnPro: 1.292 ± 0.035	0.325AsnGln: 0.325 ± 0.019	1.592AsnArg: 1.592 ± 0.047	0.578AsnSer: 0.578 ± 0.026	0.643AsnThr: 0.643 ± 0.031	1.217AsnVal: 1.217 ± 0.04	0.262AsnTrp: 0.262 ± 0.018	0.391AsnTyr: 0.391 ± 0.021	0.0AsnXaa: 0.0 ± 0.0
Pro	7.59ProAla: 7.59 ± 0.105	0.463ProCys: 0.463 ± 0.025	3.533ProAsp: 3.533 ± 0.066	5.186ProGlu: 5.186 ± 0.091	2.411ProPhe: 2.411 ± 0.043	5.328ProGly: 5.328 ± 0.078	1.285ProHis: 1.285 ± 0.036	1.963ProIle: 1.963 ± 0.054	1.135ProLys: 1.135 ± 0.041	6.559ProLeu: 6.559 ± 0.093	1.099ProMet: 1.099 ± 0.036	0.912ProAsn: 0.912 ± 0.033	4.155ProPro: 4.155 ± 0.091	1.873ProGln: 1.873 ± 0.041	4.688ProArg: 4.688 ± 0.081	2.791ProSer: 2.791 ± 0.056	2.559ProThr: 2.559 ± 0.059	5.1ProVal: 5.1 ± 0.069	0.998ProTrp: 0.998 ± 0.034	1.028ProTyr: 1.028 ± 0.03	0.0ProXaa: 0.0 ± 0.0
Gln	3.386GlnAla: 3.386 ± 0.061	0.174GlnCys: 0.174 ± 0.015	0.921GlnAsp: 0.921 ± 0.034	1.471GlnGlu: 1.471 ± 0.041	0.739GlnPhe: 0.739 ± 0.031	1.816GlnGly: 1.816 ± 0.048	0.469GlnHis: 0.469 ± 0.024	1.229GlnIle: 1.229 ± 0.04	0.608GlnLys: 0.608 ± 0.027	3.388GlnLeu: 3.388 ± 0.068	0.496GlnMet: 0.496 ± 0.021	0.408GlnAsn: 0.408 ± 0.022	1.72GlnPro: 1.72 ± 0.041	0.903GlnGln: 0.903 ± 0.039	2.703GlnArg: 2.703 ± 0.063	0.938GlnSer: 0.938 ± 0.032	1.016GlnThr: 1.016 ± 0.036	1.88GlnVal: 1.88 ± 0.051	0.308GlnTrp: 0.308 ± 0.017	0.431GlnTyr: 0.431 ± 0.022	0.0GlnXaa: 0.0 ± 0.0
Arg	11.874ArgAla: 11.874 ± 0.121	0.864ArgCys: 0.864 ± 0.033	4.788ArgAsp: 4.788 ± 0.075	7.141ArgGlu: 7.141 ± 0.103	4.069ArgPhe: 4.069 ± 0.07	6.703ArgGly: 6.703 ± 0.107	2.517ArgHis: 2.517 ± 0.062	5.24ArgIle: 5.24 ± 0.076	1.528ArgLys: 1.528 ± 0.048	13.754ArgLeu: 13.754 ± 0.158	2.037ArgMet: 2.037 ± 0.05	1.575ArgAsn: 1.575 ± 0.043	5.884ArgPro: 5.884 ± 0.096	3.164ArgGln: 3.164 ± 0.072	11.075ArgArg: 11.075 ± 0.135	4.244ArgSer: 4.244 ± 0.076	3.957ArgThr: 3.957 ± 0.067	6.879ArgVal: 6.879 ± 0.093	1.343ArgTrp: 1.343 ± 0.041	1.973ArgTyr: 1.973 ± 0.051	0.0ArgXaa: 0.0 ± 0.0
Ser	4.715SerAla: 4.715 ± 0.072	0.547SerCys: 0.547 ± 0.024	2.085SerAsp: 2.085 ± 0.057	2.571SerGlu: 2.571 ± 0.06	1.834SerPhe: 1.834 ± 0.043	4.77SerGly: 4.77 ± 0.084	0.891SerHis: 0.891 ± 0.032	1.506SerIle: 1.506 ± 0.044	0.626SerLys: 0.626 ± 0.028	5.084SerLeu: 5.084 ± 0.081	0.941SerMet: 0.941 ± 0.031	0.623SerAsn: 0.623 ± 0.027	2.876SerPro: 2.876 ± 0.059	1.017SerGln: 1.017 ± 0.032	4.07SerArg: 4.07 ± 0.078	1.959SerSer: 1.959 ± 0.046	1.797SerThr: 1.797 ± 0.049	2.801SerVal: 2.801 ± 0.065	0.746SerTrp: 0.746 ± 0.027	0.842SerTyr: 0.842 ± 0.032	0.0SerXaa: 0.0 ± 0.0
Thr	5.581ThrAla: 5.581 ± 0.086	0.419ThrCys: 0.419 ± 0.022	1.912ThrAsp: 1.912 ± 0.048	1.981ThrGlu: 1.981 ± 0.049	1.685ThrPhe: 1.685 ± 0.042	4.585ThrGly: 4.585 ± 0.078	0.85ThrHis: 0.85 ± 0.032	1.889ThrIle: 1.889 ± 0.051	0.614ThrLys: 0.614 ± 0.027	5.373ThrLeu: 5.373 ± 0.077	0.805ThrMet: 0.805 ± 0.031	0.736ThrAsn: 0.736 ± 0.035	2.847ThrPro: 2.847 ± 0.063	0.828ThrGln: 0.828 ± 0.031	3.443ThrArg: 3.443 ± 0.061	1.964ThrSer: 1.964 ± 0.052	2.164ThrThr: 2.164 ± 0.052	4.032ThrVal: 4.032 ± 0.091	0.495ThrTrp: 0.495 ± 0.025	0.775ThrTyr: 0.775 ± 0.031	0.0ThrXaa: 0.0 ± 0.0
Val	11.034ValAla: 11.034 ± 0.12	0.828ValCys: 0.828 ± 0.028	4.534ValAsp: 4.534 ± 0.069	5.868ValGlu: 5.868 ± 0.088	2.711ValPhe: 2.711 ± 0.054	5.966ValGly: 5.966 ± 0.09	1.687ValHis: 1.687 ± 0.048	3.017ValIle: 3.017 ± 0.066	1.31ValLys: 1.31 ± 0.047	9.3ValLeu: 9.3 ± 0.12	1.388ValMet: 1.388 ± 0.04	1.378ValAsn: 1.378 ± 0.04	4.785ValPro: 4.785 ± 0.075	1.817ValGln: 1.817 ± 0.048	7.512ValArg: 7.512 ± 0.105	3.137ValSer: 3.137 ± 0.069	3.807ValThr: 3.807 ± 0.075	7.521ValVal: 7.521 ± 0.11	1.014ValTrp: 1.014 ± 0.039	1.54ValTyr: 1.54 ± 0.045	0.0ValXaa: 0.0 ± 0.0
Trp	1.157TrpAla: 1.157 ± 0.036	0.128TrpCys: 0.128 ± 0.012	0.504TrpAsp: 0.504 ± 0.026	0.57TrpGlu: 0.57 ± 0.023	0.544TrpPhe: 0.544 ± 0.026	0.875TrpGly: 0.875 ± 0.037	0.365TrpHis: 0.365 ± 0.02	0.646TrpIle: 0.646 ± 0.025	0.297TrpLys: 0.297 ± 0.021	2.352TrpLeu: 2.352 ± 0.056	0.253TrpMet: 0.253 ± 0.019	0.362TrpAsn: 0.362 ± 0.019	0.939TrpPro: 0.939 ± 0.034	0.706TrpGln: 0.706 ± 0.027	1.684TrpArg: 1.684 ± 0.042	0.706TrpSer: 0.706 ± 0.027	0.66TrpThr: 0.66 ± 0.027	0.922TrpVal: 0.922 ± 0.03	0.253TrpTrp: 0.253 ± 0.019	0.335TrpTyr: 0.335 ± 0.02	0.0TrpXaa: 0.0 ± 0.0
Tyr	2.255TyrAla: 2.255 ± 0.056	0.197TyrCys: 0.197 ± 0.016	1.165TyrAsp: 1.165 ± 0.033	1.177TyrGlu: 1.177 ± 0.038	0.653TyrPhe: 0.653 ± 0.028	1.952TyrGly: 1.952 ± 0.052	0.448TyrHis: 0.448 ± 0.022	0.6TyrIle: 0.6 ± 0.025	0.298TyrLys: 0.298 ± 0.021	2.096TyrLeu: 2.096 ± 0.055	0.304TyrMet: 0.304 ± 0.02	0.356TyrAsn: 0.356 ± 0.021	1.003TyrPro: 1.003 ± 0.035	0.428TyrGln: 0.428 ± 0.02	2.127TyrArg: 2.127 ± 0.049	0.65TyrSer: 0.65 ± 0.03	0.76TyrThr: 0.76 ± 0.033	1.512TyrVal: 1.512 ± 0.049	0.298TyrTrp: 0.298 ± 0.019	0.457TyrTyr: 0.457 ± 0.023	0.0TyrXaa: 0.0 ± 0.0
Xaa	0.0XaaAla: 0.0 ± 0.0	0.0XaaCys: 0.0 ± 0.0	0.0XaaAsp: 0.0 ± 0.0	0.0XaaGlu: 0.0 ± 0.0	0.0XaaPhe: 0.0 ± 0.0	0.0XaaGly: 0.0 ± 0.0	0.0XaaHis: 0.0 ± 0.0	0.0XaaIle: 0.0 ± 0.0	0.0XaaLys: 0.0 ± 0.0	0.0XaaLeu: 0.0 ± 0.0	0.0XaaMet: 0.0 ± 0.0	0.0XaaAsn: 0.0 ± 0.0	0.0XaaPro: 0.0 ± 0.0	0.0XaaGln: 0.0 ± 0.0	0.0XaaArg: 0.0 ± 0.0	0.0XaaSer: 0.0 ± 0.0	0.0XaaThr: 0.0 ± 0.0	0.0XaaVal: 0.0 ± 0.0	0.0XaaTrp: 0.0 ± 0.0	0.0XaaTyr: 0.0 ± 0.0	0.0XaaXaa: 0.0 ± 0.0

Statistics based on 2654 proteins (892615 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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