Acinetobacter marinus
Average proteome isoelectric point is 6.25
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2744 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A1G6MZ23|A0A1G6MZ23_9GAMM 3-deoxy-D-manno-octulosonic acid transferase OS=Acinetobacter marinus OX=281375 GN=SAMN05421749_10862 PE=3 SV=1
MM1 pKa = 7.49 NKK3 pKa = 8.39 LTLATAILAASSSFAMVNANAYY25 pKa = 8.44 QFEE28 pKa = 4.48 VGAGAGYY35 pKa = 10.66 VDD37 pKa = 5.17 PDD39 pKa = 3.76 NGDD42 pKa = 3.38 SGSAFDD48 pKa = 5.98 LGGTYY53 pKa = 10.36 YY54 pKa = 10.8 FNPVEE59 pKa = 4.83 DD60 pKa = 4.13 KK61 pKa = 10.88 NAPLAEE67 pKa = 3.93 AAFVNRR73 pKa = 11.84 ASNVHH78 pKa = 6.42 AGATFQDD85 pKa = 3.82 QGDD88 pKa = 4.76 LDD90 pKa = 4.4 TNTYY94 pKa = 9.2 GAGVEE99 pKa = 4.18 YY100 pKa = 9.69 FVPNSNFYY108 pKa = 11.16 VSGDD112 pKa = 3.31 IAKK115 pKa = 9.37 TNYY118 pKa = 9.93 EE119 pKa = 4.2 YY120 pKa = 11.32 DD121 pKa = 4.39 DD122 pKa = 4.92 ADD124 pKa = 3.62 IDD126 pKa = 4.25 NDD128 pKa = 3.56 LTTYY132 pKa = 9.98 SAEE135 pKa = 4.02 VGYY138 pKa = 10.76 LPVPNLLIAVGARR151 pKa = 11.84 GWDD154 pKa = 3.41 ADD156 pKa = 4.56 DD157 pKa = 5.94 DD158 pKa = 5.12 DD159 pKa = 6.13 GVDD162 pKa = 3.22 PTLRR166 pKa = 11.84 AKK168 pKa = 10.53 YY169 pKa = 8.57 VTQIGDD175 pKa = 3.32 NAVNLEE181 pKa = 3.98 AGAAFGDD188 pKa = 3.54 LDD190 pKa = 4.55 EE191 pKa = 5.29 YY192 pKa = 11.76 NLAADD197 pKa = 3.83 YY198 pKa = 10.78 FIDD201 pKa = 3.5 NSFSVGADD209 pKa = 3.32 YY210 pKa = 11.33 YY211 pKa = 11.85 SNDD214 pKa = 3.33 LTDD217 pKa = 3.57 NSEE220 pKa = 4.04 FGINARR226 pKa = 11.84 KK227 pKa = 9.54 FITQNTSLEE236 pKa = 4.06 GRR238 pKa = 11.84 VGFGDD243 pKa = 4.34 TNDD246 pKa = 3.31 NDD248 pKa = 3.93 YY249 pKa = 11.66 NSFGIAAKK257 pKa = 10.38 HH258 pKa = 5.54 RR259 pKa = 11.84 FF260 pKa = 3.48
Molecular weight: 27.86 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.735
IPC2_protein 3.668
IPC_protein 3.706
Toseland 3.465
ProMoST 3.884
Dawson 3.706
Bjellqvist 3.859
Wikipedia 3.681
Rodwell 3.516
Grimsley 3.363
Solomon 3.706
Lehninger 3.656
Nozaki 3.821
DTASelect 4.113
Thurlkill 3.528
EMBOSS 3.681
Sillero 3.821
Patrickios 0.579
IPC_peptide 3.694
IPC2_peptide 3.795
IPC2.peptide.svr19 3.749
Protein with the highest isoelectric point:
>tr|A0A1G6NXF0|A0A1G6NXF0_9GAMM Uncharacterized protein OS=Acinetobacter marinus OX=281375 GN=SAMN05421749_11142 PE=4 SV=1
MM1 pKa = 7.45 KK2 pKa = 9.55 RR3 pKa = 11.84 TFQPSEE9 pKa = 3.97 LKK11 pKa = 10.13 RR12 pKa = 11.84 KK13 pKa = 8.98 RR14 pKa = 11.84 VHH16 pKa = 6.36 GFRR19 pKa = 11.84 ARR21 pKa = 11.84 MATKK25 pKa = 10.4 AGRR28 pKa = 11.84 QVLARR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 AKK37 pKa = 10.18 GRR39 pKa = 11.84 HH40 pKa = 5.1 SLTVV44 pKa = 3.06
Molecular weight: 5.18 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.402
IPC2_protein 10.862
IPC_protein 12.442
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.31
Grimsley 12.647
Solomon 13.1
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.047
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.077
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2744
0
2744
866506
31
1998
315.8
35.23
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
9.059 ± 0.054
0.957 ± 0.016
5.658 ± 0.04
5.649 ± 0.051
4.141 ± 0.037
6.39 ± 0.05
2.521 ± 0.029
6.532 ± 0.035
5.123 ± 0.04
10.147 ± 0.06
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.602 ± 0.026
4.27 ± 0.034
3.855 ± 0.025
5.957 ± 0.055
4.486 ± 0.037
6.207 ± 0.039
5.389 ± 0.044
6.597 ± 0.039
1.254 ± 0.018
3.205 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here