Xanthomonas phage XacN1
Average proteome isoelectric point is 6.31
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 459 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A292GIX2|A0A292GIX2_9CAUD Predicted ORF OS=Xanthomonas phage XacN1 OX=2042251 PE=4 SV=1
MM1 pKa = 7.1 SQRR4 pKa = 11.84 ISDD7 pKa = 4.28 LTPVSSIAGDD17 pKa = 3.63 EE18 pKa = 4.15 LVEE21 pKa = 4.16 VAVPNSEE28 pKa = 4.19 SPTGYY33 pKa = 9.94 VSRR36 pKa = 11.84 SASTSLFGGGGGLPYY51 pKa = 10.72 RR52 pKa = 11.84 FTFEE56 pKa = 5.23 INDD59 pKa = 3.64 EE60 pKa = 4.77 PIGSDD65 pKa = 4.79 LSFQLYY71 pKa = 10.06 LEE73 pKa = 4.76 KK74 pKa = 11.09 GQFDD78 pKa = 3.82 STAFRR83 pKa = 11.84 PNADD87 pKa = 3.92 SDD89 pKa = 4.3 PDD91 pKa = 4.35 GDD93 pKa = 3.72 WTLATAIMNFNAIAGFHH110 pKa = 6.53 FSSEE114 pKa = 4.52 DD115 pKa = 3.16 NFQFYY120 pKa = 10.94 AFSPLNSSVWVNKK133 pKa = 9.72 MGGALGSGADD143 pKa = 3.63 SDD145 pKa = 4.95 ADD147 pKa = 3.44 QLRR150 pKa = 11.84 WGTFNNSNDD159 pKa = 3.12 LSIYY163 pKa = 9.44 QIPDD167 pKa = 3.16 NYY169 pKa = 10.31 PSSNYY174 pKa = 9.27 MNNTPVIAPVEE185 pKa = 4.29 VYY187 pKa = 10.69 EE188 pKa = 5.28 DD189 pKa = 4.2 GNFGPDD195 pKa = 3.09 GSIVIRR201 pKa = 11.84 FRR203 pKa = 11.84 VVSPYY208 pKa = 10.6 NGWGTYY214 pKa = 8.91 PQTFSLKK221 pKa = 10.2 GYY223 pKa = 10.53 ADD225 pKa = 3.21 INMAFSNFYY234 pKa = 10.67 RR235 pKa = 11.84 PAA237 pKa = 5.35
Molecular weight: 25.87 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.768
IPC2_protein 3.961
IPC_protein 3.948
Toseland 3.732
ProMoST 4.113
Dawson 3.935
Bjellqvist 4.088
Wikipedia 3.884
Rodwell 3.77
Grimsley 3.63
Solomon 3.935
Lehninger 3.884
Nozaki 4.05
DTASelect 4.304
Thurlkill 3.783
EMBOSS 3.884
Sillero 4.062
Patrickios 1.926
IPC_peptide 3.923
IPC2_peptide 4.037
IPC2.peptide.svr19 3.955
Protein with the highest isoelectric point:
>tr|A0A292GI63|A0A292GI63_9CAUD Predicted ORF OS=Xanthomonas phage XacN1 OX=2042251 PE=4 SV=1
MM1 pKa = 7.73 SIEE4 pKa = 4.08 ATIMGYY10 pKa = 7.79 FHH12 pKa = 7.55 EE13 pKa = 4.93 YY14 pKa = 9.34 DD15 pKa = 3.25 AKK17 pKa = 11.09 ARR19 pKa = 11.84 ALYY22 pKa = 8.74 PAYY25 pKa = 10.18 AARR28 pKa = 11.84 PVPKK32 pKa = 10.29 VSFFAKK38 pKa = 10.09 GRR40 pKa = 11.84 MNGWAKK46 pKa = 9.8 QGSWEE51 pKa = 4.32 VQFNTHH57 pKa = 6.11 NASQVPEE64 pKa = 4.1 EE65 pKa = 3.93 FRR67 pKa = 11.84 NTVSHH72 pKa = 7.09 EE73 pKa = 3.78 IAHH76 pKa = 5.65 MVDD79 pKa = 3.0 YY80 pKa = 10.9 ALRR83 pKa = 11.84 GRR85 pKa = 11.84 SGHH88 pKa = 5.59 DD89 pKa = 3.69 RR90 pKa = 11.84 IWKK93 pKa = 9.55 AIHH96 pKa = 6.61 RR97 pKa = 11.84 SLGGTGTRR105 pKa = 11.84 CSQYY109 pKa = 10.76 GITVKK114 pKa = 10.43 RR115 pKa = 11.84 GRR117 pKa = 11.84 VKK119 pKa = 10.93 NEE121 pKa = 3.27 YY122 pKa = 10.35 LYY124 pKa = 11.19 RR125 pKa = 11.84 NANGAEE131 pKa = 3.96 TWAGPKK137 pKa = 9.38 HH138 pKa = 5.55 HH139 pKa = 7.27 AAMQRR144 pKa = 11.84 CSIAGIRR151 pKa = 11.84 NRR153 pKa = 11.84 SGQVFNRR160 pKa = 11.84 NDD162 pKa = 2.8 WTGQRR167 pKa = 11.84 RR168 pKa = 11.84 TIGG171 pKa = 3.22
Molecular weight: 19.41 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.319
IPC2_protein 9.78
IPC_protein 10.511
Toseland 10.394
ProMoST 10.16
Dawson 10.584
Bjellqvist 10.306
Wikipedia 10.804
Rodwell 10.789
Grimsley 10.657
Solomon 10.643
Lehninger 10.599
Nozaki 10.379
DTASelect 10.306
Thurlkill 10.438
EMBOSS 10.804
Sillero 10.496
Patrickios 10.438
IPC_peptide 10.643
IPC2_peptide 9.297
IPC2.peptide.svr19 8.494
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
459
0
459
96337
35
3313
209.9
23.62
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.473 ± 0.136
0.949 ± 0.049
6.409 ± 0.125
6.472 ± 0.216
4.389 ± 0.086
6.528 ± 0.154
2.005 ± 0.098
5.875 ± 0.087
6.222 ± 0.249
7.594 ± 0.163
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.523 ± 0.078
5.122 ± 0.116
4.625 ± 0.185
3.439 ± 0.094
4.773 ± 0.113
6.078 ± 0.165
7.095 ± 0.408
6.775 ± 0.116
1.487 ± 0.06
4.167 ± 0.097
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here