Amino acid dipepetide frequency for Xanthomonas phage XacN1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.747AlaAla: 6.747 ± 0.359
0.747AlaCys: 0.747 ± 0.092
4.713AlaAsp: 4.713 ± 0.211
4.796AlaGlu: 4.796 ± 0.315
3.208AlaPhe: 3.208 ± 0.168
4.754AlaGly: 4.754 ± 0.287
1.235AlaHis: 1.235 ± 0.113
4.453AlaIle: 4.453 ± 0.198
4.713AlaLys: 4.713 ± 0.273
6.083AlaLeu: 6.083 ± 0.309
2.086AlaMet: 2.086 ± 0.159
3.612AlaAsn: 3.612 ± 0.204
2.896AlaPro: 2.896 ± 0.241
2.585AlaGln: 2.585 ± 0.193
3.612AlaArg: 3.612 ± 0.196
4.318AlaSer: 4.318 ± 0.222
5.252AlaThr: 5.252 ± 0.358
5.003AlaVal: 5.003 ± 0.248
0.965AlaTrp: 0.965 ± 0.108
2.948AlaTyr: 2.948 ± 0.168
0.0AlaXaa: 0.0 ± 0.0
Cys
0.467CysAla: 0.467 ± 0.082
0.114CysCys: 0.114 ± 0.035
0.602CysAsp: 0.602 ± 0.082
0.716CysGlu: 0.716 ± 0.088
0.322CysPhe: 0.322 ± 0.055
0.851CysGly: 0.851 ± 0.101
0.291CysHis: 0.291 ± 0.044
0.446CysIle: 0.446 ± 0.069
0.716CysLys: 0.716 ± 0.093
0.592CysLeu: 0.592 ± 0.08
0.249CysMet: 0.249 ± 0.05
0.436CysAsn: 0.436 ± 0.063
0.509CysPro: 0.509 ± 0.059
0.26CysGln: 0.26 ± 0.052
0.477CysArg: 0.477 ± 0.071
0.862CysSer: 0.862 ± 0.123
0.498CysThr: 0.498 ± 0.066
0.571CysVal: 0.571 ± 0.078
0.125CysTrp: 0.125 ± 0.042
0.384CysTyr: 0.384 ± 0.066
0.0CysXaa: 0.0 ± 0.0
Asp
5.553AspAla: 5.553 ± 0.214
0.664AspCys: 0.664 ± 0.095
3.986AspAsp: 3.986 ± 0.295
4.464AspGlu: 4.464 ± 0.236
3.021AspPhe: 3.021 ± 0.182
5.097AspGly: 5.097 ± 0.277
1.163AspHis: 1.163 ± 0.112
3.945AspIle: 3.945 ± 0.189
4.069AspLys: 4.069 ± 0.308
4.64AspLeu: 4.64 ± 0.227
1.785AspMet: 1.785 ± 0.158
2.938AspAsn: 2.938 ± 0.187
2.481AspPro: 2.481 ± 0.192
1.474AspGln: 1.474 ± 0.121
2.678AspArg: 2.678 ± 0.163
3.913AspSer: 3.913 ± 0.21
3.861AspThr: 3.861 ± 0.231
4.028AspVal: 4.028 ± 0.223
1.121AspTrp: 1.121 ± 0.106
3.208AspTyr: 3.208 ± 0.195
0.0AspXaa: 0.0 ± 0.0
Glu
5.149GluAla: 5.149 ± 0.31
0.81GluCys: 0.81 ± 0.092
4.495GluAsp: 4.495 ± 0.252
4.557GluGlu: 4.557 ± 0.375
3.415GluPhe: 3.415 ± 0.228
3.394GluGly: 3.394 ± 0.202
1.391GluHis: 1.391 ± 0.131
4.287GluIle: 4.287 ± 0.258
4.111GluLys: 4.111 ± 0.293
6.187GluLeu: 6.187 ± 0.311
1.557GluMet: 1.557 ± 0.13
2.543GluAsn: 2.543 ± 0.142
1.91GluPro: 1.91 ± 0.154
2.699GluGln: 2.699 ± 0.192
3.394GluArg: 3.394 ± 0.24
3.394GluSer: 3.394 ± 0.198
3.301GluThr: 3.301 ± 0.205
4.017GluVal: 4.017 ± 0.239
1.152GluTrp: 1.152 ± 0.118
2.958GluTyr: 2.958 ± 0.189
0.0GluXaa: 0.0 ± 0.0
Phe
3.249PheAla: 3.249 ± 0.191
0.446PheCys: 0.446 ± 0.06
3.592PheAsp: 3.592 ± 0.235
3.124PheGlu: 3.124 ± 0.212
1.64PhePhe: 1.64 ± 0.123
2.73PheGly: 2.73 ± 0.165
1.059PheHis: 1.059 ± 0.116
2.616PheIle: 2.616 ± 0.162
3.052PheLys: 3.052 ± 0.224
3.021PheLeu: 3.021 ± 0.149
1.152PheMet: 1.152 ± 0.11
2.471PheAsn: 2.471 ± 0.207
1.702PhePro: 1.702 ± 0.146
1.505PheGln: 1.505 ± 0.124
1.993PheArg: 1.993 ± 0.167
2.657PheSer: 2.657 ± 0.179
2.969PheThr: 2.969 ± 0.209
2.605PheVal: 2.605 ± 0.182
0.654PheTrp: 0.654 ± 0.076
1.65PheTyr: 1.65 ± 0.156
0.0PheXaa: 0.0 ± 0.0
Gly
3.82GlyAla: 3.82 ± 0.29
0.633GlyCys: 0.633 ± 0.082
4.121GlyAsp: 4.121 ± 0.19
3.893GlyGlu: 3.893 ± 0.228
2.979GlyPhe: 2.979 ± 0.196
4.796GlyGly: 4.796 ± 0.437
1.183GlyHis: 1.183 ± 0.109
3.903GlyIle: 3.903 ± 0.186
4.09GlyLys: 4.09 ± 0.209
4.765GlyLeu: 4.765 ± 0.249
1.516GlyMet: 1.516 ± 0.118
3.571GlyAsn: 3.571 ± 0.226
1.806GlyPro: 1.806 ± 0.157
2.699GlyGln: 2.699 ± 0.265
3.332GlyArg: 3.332 ± 0.21
4.152GlySer: 4.152 ± 0.232
4.848GlyThr: 4.848 ± 0.348
4.422GlyVal: 4.422 ± 0.21
1.277GlyTrp: 1.277 ± 0.118
3.477GlyTyr: 3.477 ± 0.2
0.0GlyXaa: 0.0 ± 0.0
His
1.308HisAla: 1.308 ± 0.118
0.311HisCys: 0.311 ± 0.062
1.266HisAsp: 1.266 ± 0.121
1.329HisGlu: 1.329 ± 0.12
0.965HisPhe: 0.965 ± 0.107
1.536HisGly: 1.536 ± 0.132
0.592HisHis: 0.592 ± 0.083
1.318HisIle: 1.318 ± 0.139
0.893HisLys: 0.893 ± 0.105
1.391HisLeu: 1.391 ± 0.112
0.571HisMet: 0.571 ± 0.072
1.163HisAsn: 1.163 ± 0.119
0.913HisPro: 0.913 ± 0.111
0.519HisGln: 0.519 ± 0.079
1.246HisArg: 1.246 ± 0.107
1.017HisSer: 1.017 ± 0.114
1.069HisThr: 1.069 ± 0.106
1.453HisVal: 1.453 ± 0.133
0.28HisTrp: 0.28 ± 0.053
0.913HisTyr: 0.913 ± 0.089
0.0HisXaa: 0.0 ± 0.0
Ile
4.464IleAla: 4.464 ± 0.214
0.561IleCys: 0.561 ± 0.077
3.83IleAsp: 3.83 ± 0.231
3.913IleGlu: 3.913 ± 0.252
2.097IlePhe: 2.097 ± 0.149
3.56IleGly: 3.56 ± 0.184
1.194IleHis: 1.194 ± 0.121
3.436IleIle: 3.436 ± 0.213
3.727IleLys: 3.727 ± 0.225
4.183IleLeu: 4.183 ± 0.237
1.495IleMet: 1.495 ± 0.14
2.886IleAsn: 2.886 ± 0.192
3.405IlePro: 3.405 ± 0.174
2.18IleGln: 2.18 ± 0.163
3.021IleArg: 3.021 ± 0.19
3.706IleSer: 3.706 ± 0.21
4.432IleThr: 4.432 ± 0.307
4.069IleVal: 4.069 ± 0.198
0.612IleTrp: 0.612 ± 0.072
1.983IleTyr: 1.983 ± 0.147
0.0IleXaa: 0.0 ± 0.0
Lys
4.692LysAla: 4.692 ± 0.292
0.581LysCys: 0.581 ± 0.096
3.643LysAsp: 3.643 ± 0.275
4.63LysGlu: 4.63 ± 0.309
3.062LysPhe: 3.062 ± 0.216
3.197LysGly: 3.197 ± 0.241
1.432LysHis: 1.432 ± 0.128
3.259LysIle: 3.259 ± 0.22
4.972LysLys: 4.972 ± 0.419
5.813LysLeu: 5.813 ± 0.344
1.827LysMet: 1.827 ± 0.137
3.291LysAsn: 3.291 ± 0.201
2.419LysPro: 2.419 ± 0.181
2.128LysGln: 2.128 ± 0.187
2.979LysArg: 2.979 ± 0.185
3.519LysSer: 3.519 ± 0.21
3.394LysThr: 3.394 ± 0.171
3.778LysVal: 3.778 ± 0.238
0.934LysTrp: 0.934 ± 0.106
2.668LysTyr: 2.668 ± 0.201
0.0LysXaa: 0.0 ± 0.0
Leu
5.979LeuAla: 5.979 ± 0.308
0.633LeuCys: 0.633 ± 0.086
4.993LeuAsp: 4.993 ± 0.236
4.91LeuGlu: 4.91 ± 0.305
3.114LeuPhe: 3.114 ± 0.161
4.682LeuGly: 4.682 ± 0.226
1.609LeuHis: 1.609 ± 0.121
3.716LeuIle: 3.716 ± 0.228
4.806LeuLys: 4.806 ± 0.338
5.699LeuLeu: 5.699 ± 0.34
2.014LeuMet: 2.014 ± 0.144
4.661LeuAsn: 4.661 ± 0.276
3.675LeuPro: 3.675 ± 0.174
2.512LeuGln: 2.512 ± 0.172
3.747LeuArg: 3.747 ± 0.173
5.201LeuSer: 5.201 ± 0.256
5.211LeuThr: 5.211 ± 0.232
5.055LeuVal: 5.055 ± 0.256
0.945LeuTrp: 0.945 ± 0.106
2.782LeuTyr: 2.782 ± 0.192
0.0LeuXaa: 0.0 ± 0.0
Met
2.201MetAla: 2.201 ± 0.161
0.228MetCys: 0.228 ± 0.046
1.63MetAsp: 1.63 ± 0.14
1.453MetGlu: 1.453 ± 0.146
1.163MetPhe: 1.163 ± 0.116
1.516MetGly: 1.516 ± 0.12
0.581MetHis: 0.581 ± 0.07
1.08MetIle: 1.08 ± 0.113
1.858MetLys: 1.858 ± 0.161
1.848MetLeu: 1.848 ± 0.147
0.581MetMet: 0.581 ± 0.087
1.318MetAsn: 1.318 ± 0.106
0.872MetPro: 0.872 ± 0.084
0.976MetGln: 0.976 ± 0.095
1.464MetArg: 1.464 ± 0.121
1.952MetSer: 1.952 ± 0.121
2.076MetThr: 2.076 ± 0.135
1.453MetVal: 1.453 ± 0.115
0.343MetTrp: 0.343 ± 0.056
0.644MetTyr: 0.644 ± 0.088
0.0MetXaa: 0.0 ± 0.0
Asn
3.643AsnAla: 3.643 ± 0.19
0.457AsnCys: 0.457 ± 0.075
3.0AsnAsp: 3.0 ± 0.196
2.834AsnGlu: 2.834 ± 0.174
2.086AsnPhe: 2.086 ± 0.198
4.671AsnGly: 4.671 ± 0.294
0.913AsnHis: 0.913 ± 0.081
3.218AsnIle: 3.218 ± 0.195
2.834AsnLys: 2.834 ± 0.203
3.789AsnLeu: 3.789 ± 0.205
1.038AsnMet: 1.038 ± 0.12
2.419AsnAsn: 2.419 ± 0.218
2.958AsnPro: 2.958 ± 0.171
1.557AsnGln: 1.557 ± 0.143
2.554AsnArg: 2.554 ± 0.189
3.176AsnSer: 3.176 ± 0.222
3.239AsnThr: 3.239 ± 0.247
3.695AsnVal: 3.695 ± 0.226
0.945AsnTrp: 0.945 ± 0.101
2.19AsnTyr: 2.19 ± 0.166
0.0AsnXaa: 0.0 ± 0.0
Pro
3.353ProAla: 3.353 ± 0.223
0.301ProCys: 0.301 ± 0.058
2.958ProAsp: 2.958 ± 0.189
3.156ProGlu: 3.156 ± 0.197
1.817ProPhe: 1.817 ± 0.143
2.398ProGly: 2.398 ± 0.164
0.882ProHis: 0.882 ± 0.098
2.18ProIle: 2.18 ± 0.143
2.377ProLys: 2.377 ± 0.158
2.647ProLeu: 2.647 ± 0.166
0.903ProMet: 0.903 ± 0.097
2.325ProAsn: 2.325 ± 0.158
1.692ProPro: 1.692 ± 0.151
1.588ProGln: 1.588 ± 0.136
1.557ProArg: 1.557 ± 0.119
3.789ProSer: 3.789 ± 0.356
6.602ProThr: 6.602 ± 0.999
3.509ProVal: 3.509 ± 0.185
0.488ProTrp: 0.488 ± 0.062
1.734ProTyr: 1.734 ± 0.165
0.0ProXaa: 0.0 ± 0.0
Gln
2.564GlnAla: 2.564 ± 0.21
0.26GlnCys: 0.26 ± 0.054
1.879GlnAsp: 1.879 ± 0.134
2.294GlnGlu: 2.294 ± 0.175
1.806GlnPhe: 1.806 ± 0.128
2.035GlnGly: 2.035 ± 0.217
0.55GlnHis: 0.55 ± 0.074
2.253GlnIle: 2.253 ± 0.17
2.221GlnLys: 2.221 ± 0.169
2.782GlnLeu: 2.782 ± 0.176
0.913GlnMet: 0.913 ± 0.093
1.827GlnAsn: 1.827 ± 0.161
1.37GlnPro: 1.37 ± 0.12
1.765GlnGln: 1.765 ± 0.241
1.837GlnArg: 1.837 ± 0.16
1.734GlnSer: 1.734 ± 0.137
2.19GlnThr: 2.19 ± 0.159
2.097GlnVal: 2.097 ± 0.161
0.509GlnTrp: 0.509 ± 0.086
1.505GlnTyr: 1.505 ± 0.119
0.0GlnXaa: 0.0 ± 0.0
Arg
3.228ArgAla: 3.228 ± 0.198
0.519ArgCys: 0.519 ± 0.064
2.647ArgAsp: 2.647 ± 0.157
3.415ArgGlu: 3.415 ± 0.238
2.086ArgPhe: 2.086 ± 0.133
3.093ArgGly: 3.093 ± 0.204
1.017ArgHis: 1.017 ± 0.105
3.093ArgIle: 3.093 ± 0.199
3.581ArgLys: 3.581 ± 0.241
3.934ArgLeu: 3.934 ± 0.197
1.308ArgMet: 1.308 ± 0.122
2.709ArgAsn: 2.709 ± 0.149
1.775ArgPro: 1.775 ± 0.123
1.578ArgGln: 1.578 ± 0.114
2.273ArgArg: 2.273 ± 0.177
2.273ArgSer: 2.273 ± 0.164
3.135ArgThr: 3.135 ± 0.213
3.208ArgVal: 3.208 ± 0.167
0.82ArgTrp: 0.82 ± 0.091
2.024ArgTyr: 2.024 ± 0.139
0.0ArgXaa: 0.0 ± 0.0
Ser
4.619SerAla: 4.619 ± 0.295
0.488SerCys: 0.488 ± 0.072
4.401SerAsp: 4.401 ± 0.201
3.654SerGlu: 3.654 ± 0.223
2.595SerPhe: 2.595 ± 0.178
4.183SerGly: 4.183 ± 0.272
1.09SerHis: 1.09 ± 0.123
4.142SerIle: 4.142 ± 0.223
3.322SerLys: 3.322 ± 0.216
4.588SerLeu: 4.588 ± 0.218
1.401SerMet: 1.401 ± 0.135
3.062SerAsn: 3.062 ± 0.212
3.135SerPro: 3.135 ± 0.245
2.014SerGln: 2.014 ± 0.15
2.689SerArg: 2.689 ± 0.165
3.56SerSer: 3.56 ± 0.292
4.059SerThr: 4.059 ± 0.368
4.401SerVal: 4.401 ± 0.301
0.83SerTrp: 0.83 ± 0.093
2.543SerTyr: 2.543 ± 0.18
0.0SerXaa: 0.0 ± 0.0
Thr
4.9ThrAla: 4.9 ± 0.318
0.55ThrCys: 0.55 ± 0.083
4.017ThrAsp: 4.017 ± 0.198
3.643ThrGlu: 3.643 ± 0.19
3.135ThrPhe: 3.135 ± 0.182
4.713ThrGly: 4.713 ± 0.295
1.277ThrHis: 1.277 ± 0.108
4.702ThrIle: 4.702 ± 0.351
3.664ThrLys: 3.664 ± 0.206
4.941ThrLeu: 4.941 ± 0.25
1.713ThrMet: 1.713 ± 0.118
2.938ThrAsn: 2.938 ± 0.257
7.858ThrPro: 7.858 ± 1.27
2.325ThrGln: 2.325 ± 0.167
2.803ThrArg: 2.803 ± 0.254
3.913ThrSer: 3.913 ± 0.348
4.495ThrThr: 4.495 ± 0.373
5.699ThrVal: 5.699 ± 0.446
1.007ThrTrp: 1.007 ± 0.131
2.657ThrTyr: 2.657 ± 0.193
0.0ThrXaa: 0.0 ± 0.0
Val
5.211ValAla: 5.211 ± 0.29
0.467ValCys: 0.467 ± 0.062
4.308ValAsp: 4.308 ± 0.237
4.65ValGlu: 4.65 ± 0.271
2.543ValPhe: 2.543 ± 0.161
4.37ValGly: 4.37 ± 0.25
1.287ValHis: 1.287 ± 0.105
3.727ValIle: 3.727 ± 0.207
4.007ValLys: 4.007 ± 0.262
4.775ValLeu: 4.775 ± 0.223
1.692ValMet: 1.692 ± 0.129
3.55ValAsn: 3.55 ± 0.204
3.0ValPro: 3.0 ± 0.188
2.003ValGln: 2.003 ± 0.166
2.99ValArg: 2.99 ± 0.173
4.09ValSer: 4.09 ± 0.235
6.342ValThr: 6.342 ± 0.653
4.9ValVal: 4.9 ± 0.256
1.007ValTrp: 1.007 ± 0.11
2.834ValTyr: 2.834 ± 0.158
0.0ValXaa: 0.0 ± 0.0
Trp
0.945TrpAla: 0.945 ± 0.105
0.26TrpCys: 0.26 ± 0.049
0.934TrpAsp: 0.934 ± 0.099
0.924TrpGlu: 0.924 ± 0.102
0.882TrpPhe: 0.882 ± 0.1
0.882TrpGly: 0.882 ± 0.103
0.436TrpHis: 0.436 ± 0.067
0.706TrpIle: 0.706 ± 0.08
0.841TrpLys: 0.841 ± 0.095
1.142TrpLeu: 1.142 ± 0.116
0.374TrpMet: 0.374 ± 0.063
0.965TrpAsn: 0.965 ± 0.125
0.291TrpPro: 0.291 ± 0.049
0.529TrpGln: 0.529 ± 0.064
0.695TrpArg: 0.695 ± 0.086
1.007TrpSer: 1.007 ± 0.151
1.059TrpThr: 1.059 ± 0.108
0.841TrpVal: 0.841 ± 0.11
0.311TrpTrp: 0.311 ± 0.053
0.851TrpTyr: 0.851 ± 0.089
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.637TyrAla: 2.637 ± 0.163
0.457TyrCys: 0.457 ± 0.064
3.073TyrAsp: 3.073 ± 0.244
2.367TyrGlu: 2.367 ± 0.166
1.952TyrPhe: 1.952 ± 0.178
2.823TyrGly: 2.823 ± 0.154
0.872TyrHis: 0.872 ± 0.108
2.273TyrIle: 2.273 ± 0.149
2.367TyrLys: 2.367 ± 0.159
3.124TyrLeu: 3.124 ± 0.182
1.048TyrMet: 1.048 ± 0.121
2.533TyrAsn: 2.533 ± 0.206
1.588TyrPro: 1.588 ± 0.141
1.495TyrGln: 1.495 ± 0.136
2.387TyrArg: 2.387 ± 0.174
2.543TyrSer: 2.543 ± 0.148
2.917TyrThr: 2.917 ± 0.158
2.948TyrVal: 2.948 ± 0.175
0.55TyrTrp: 0.55 ± 0.078
1.713TyrTyr: 1.713 ± 0.184
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 459 proteins (96337 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski