Marinirhabdus gelatinilytica
Average proteome isoelectric point is 6.4
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3022 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A370QGB2|A0A370QGB2_9FLAO Phosphate starvation-inducible protein PhoH OS=Marinirhabdus gelatinilytica OX=1703343 GN=C8D94_102583 PE=4 SV=1
MM1 pKa = 7.48 KK2 pKa = 10.4 KK3 pKa = 9.77 ILLTVLGTLALFSCSKK19 pKa = 10.49 DD20 pKa = 3.55 SEE22 pKa = 4.66 VLTTTSQDD30 pKa = 3.3 LTVEE34 pKa = 4.55 LAQEE38 pKa = 4.2 EE39 pKa = 4.67 KK40 pKa = 10.78 SVFGTYY46 pKa = 10.17 KK47 pKa = 11.02 GIFTTNNAEE56 pKa = 3.93 YY57 pKa = 10.44 RR58 pKa = 11.84 GTFEE62 pKa = 4.68 LWVPSSNSSSYY73 pKa = 10.76 SISAYY78 pKa = 9.81 RR79 pKa = 11.84 VPTVKK84 pKa = 9.7 LTLHH88 pKa = 5.74 TGEE91 pKa = 4.89 VYY93 pKa = 10.79 FSQAEE98 pKa = 4.26 SNPVGGRR105 pKa = 11.84 ALSNLEE111 pKa = 3.96 FVSDD115 pKa = 4.08 DD116 pKa = 3.44 FTFSFSVNEE125 pKa = 4.62 DD126 pKa = 2.94 GSNPTVTNVTFKK138 pKa = 10.85 NAEE141 pKa = 3.74 GDD143 pKa = 3.61 MLLAKK148 pKa = 8.89 HH149 pKa = 5.37 TQRR152 pKa = 11.84 APVNPRR158 pKa = 11.84 SGTWDD163 pKa = 3.29 CTTCNGHH170 pKa = 6.31 PVVGQGGTQTFNMMFVTADD189 pKa = 3.45 GNSTITTQSTINSTTTDD206 pKa = 4.13 GIGVQSNCMANGDD219 pKa = 4.03 TTVCTIEE226 pKa = 4.83 SGDD229 pKa = 3.7 GTTTTTGFLSNGNPVTWLGTHH250 pKa = 6.26 TFNNEE255 pKa = 2.97 VSGPDD260 pKa = 3.67 DD261 pKa = 4.34 CSTLQGTWEE270 pKa = 4.28 WEE272 pKa = 4.27 SISYY276 pKa = 7.71 GTLEE280 pKa = 4.45 GNFITDD286 pKa = 2.99 IAGNCPITDD295 pKa = 3.22 VTLINEE301 pKa = 4.44 DD302 pKa = 3.94 FEE304 pKa = 5.05 DD305 pKa = 3.7 ATVSYY310 pKa = 10.57 VASFDD315 pKa = 4.1 DD316 pKa = 5.37 LSGEE320 pKa = 3.73 NDD322 pKa = 3.52 DD323 pKa = 4.92 YY324 pKa = 11.74 FRR326 pKa = 11.84 ITNGTDD332 pKa = 2.73 ISATINNVQGSQFFAAQDD350 pKa = 3.56 TDD352 pKa = 3.78 GVAGNGQSPANLTWSNLNITGLTNINFSAFFAEE385 pKa = 5.45 DD386 pKa = 3.79 DD387 pKa = 4.12 ASDD390 pKa = 4.94 AAEE393 pKa = 4.53 DD394 pKa = 3.46 WDD396 pKa = 3.85 NADD399 pKa = 4.36 FVDD402 pKa = 3.83 VQYY405 pKa = 11.6 SFDD408 pKa = 3.43 GTTYY412 pKa = 8.02 TTFFSIRR419 pKa = 11.84 NDD421 pKa = 2.81 GSTFNAAPQIDD432 pKa = 4.1 TDD434 pKa = 4.32 LDD436 pKa = 4.12 GVGDD440 pKa = 3.9 GAIITDD446 pKa = 3.3 NMANYY451 pKa = 9.79 SASFPINGTTNPTASTEE468 pKa = 3.47 LWIRR472 pKa = 11.84 VNVGLDD478 pKa = 3.27 SGDD481 pKa = 3.46 EE482 pKa = 4.61 DD483 pKa = 4.13 IAFDD487 pKa = 4.07 DD488 pKa = 5.62 FIVIGNN494 pKa = 3.95
Molecular weight: 52.92 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.694
IPC2_protein 3.63
IPC_protein 3.668
Toseland 3.439
ProMoST 3.834
Dawson 3.668
Bjellqvist 3.821
Wikipedia 3.605
Rodwell 3.49
Grimsley 3.338
Solomon 3.656
Lehninger 3.617
Nozaki 3.77
DTASelect 4.037
Thurlkill 3.49
EMBOSS 3.617
Sillero 3.795
Patrickios 1.291
IPC_peptide 3.656
IPC2_peptide 3.77
IPC2.peptide.svr19 3.71
Protein with the highest isoelectric point:
>tr|A0A370QFD6|A0A370QFD6_9FLAO Uncharacterized protein (DUF58 family) OS=Marinirhabdus gelatinilytica OX=1703343 GN=C8D94_102258 PE=4 SV=1
MM1 pKa = 7.53 IKK3 pKa = 10.29 FFRR6 pKa = 11.84 HH7 pKa = 4.03 IRR9 pKa = 11.84 QRR11 pKa = 11.84 LIRR14 pKa = 11.84 EE15 pKa = 4.01 NRR17 pKa = 11.84 FSKK20 pKa = 10.73 YY21 pKa = 10.02 LLYY24 pKa = 10.77 AIGEE28 pKa = 4.34 IILVVIGILIAEE40 pKa = 4.33 NLAPSLKK47 pKa = 10.72 GGFSKK52 pKa = 11.05 DD53 pKa = 3.34 PLRR56 pKa = 5.7
Molecular weight: 6.53 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.312
IPC2_protein 9.838
IPC_protein 10.643
Toseland 10.804
ProMoST 10.965
Dawson 10.891
Bjellqvist 10.584
Wikipedia 11.096
Rodwell 11.169
Grimsley 10.935
Solomon 11.008
Lehninger 10.979
Nozaki 10.76
DTASelect 10.599
Thurlkill 10.789
EMBOSS 11.199
Sillero 10.818
Patrickios 10.95
IPC_peptide 11.023
IPC2_peptide 9.355
IPC2.peptide.svr19 8.675
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3022
0
3022
992534
28
2692
328.4
36.95
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
6.758 ± 0.034
0.744 ± 0.016
5.588 ± 0.061
6.947 ± 0.043
5.176 ± 0.037
6.671 ± 0.054
1.764 ± 0.022
7.258 ± 0.042
7.243 ± 0.068
9.226 ± 0.054
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.192 ± 0.026
5.808 ± 0.041
3.58 ± 0.031
3.633 ± 0.023
3.61 ± 0.032
6.059 ± 0.034
6.245 ± 0.041
6.527 ± 0.031
1.03 ± 0.016
3.941 ± 0.03
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here