Lactobacillus rodentium
Average proteome isoelectric point is 6.92
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1434 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z6TG48|A0A2Z6TG48_9LACO DegV family protein OS=Lactobacillus rodentium OX=947835 GN=degV PE=4 SV=1
MM1 pKa = 7.78 IIFITSITTYY11 pKa = 9.49 TFDD14 pKa = 3.82 FARR17 pKa = 11.84 LLVDD21 pKa = 3.89 MIEE24 pKa = 4.0 NDD26 pKa = 2.95 KK27 pKa = 11.02 YY28 pKa = 11.3 GYY30 pKa = 9.94 YY31 pKa = 10.24 HH32 pKa = 6.84 ATNAEE37 pKa = 4.1 LPDD40 pKa = 3.86 TEE42 pKa = 4.99 SDD44 pKa = 3.14 HH45 pKa = 7.63 DD46 pKa = 4.25 EE47 pKa = 4.37 NGTLRR52 pKa = 11.84 LATFF56 pKa = 4.48
Molecular weight: 6.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.905
IPC2_protein 4.228
IPC_protein 4.088
Toseland 3.897
ProMoST 4.253
Dawson 4.075
Bjellqvist 4.228
Wikipedia 4.012
Rodwell 3.923
Grimsley 3.821
Solomon 4.062
Lehninger 4.012
Nozaki 4.202
DTASelect 4.406
Thurlkill 3.961
EMBOSS 4.024
Sillero 4.215
Patrickios 1.964
IPC_peptide 4.062
IPC2_peptide 4.19
IPC2.peptide.svr19 4.096
Protein with the highest isoelectric point:
>tr|A0A2Z6T855|A0A2Z6T855_9LACO Pyruvate kinase OS=Lactobacillus rodentium OX=947835 GN=pykF PE=3 SV=1
MM1 pKa = 6.05 TTKK4 pKa = 9.88 RR5 pKa = 11.84 TYY7 pKa = 9.64 QPKK10 pKa = 8.89 KK11 pKa = 7.38 RR12 pKa = 11.84 HH13 pKa = 5.81 RR14 pKa = 11.84 SRR16 pKa = 11.84 VHH18 pKa = 6.84 GFMKK22 pKa = 10.51 RR23 pKa = 11.84 MATSNGRR30 pKa = 11.84 KK31 pKa = 8.81 VLARR35 pKa = 11.84 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 9.2 KK39 pKa = 10.05 GRR41 pKa = 11.84 KK42 pKa = 8.66 VLSAA46 pKa = 4.05
Molecular weight: 5.5 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.415
IPC2_protein 11.096
IPC_protein 12.457
Toseland 12.618
ProMoST 13.1
Dawson 12.618
Bjellqvist 12.603
Wikipedia 13.086
Rodwell 12.398
Grimsley 12.661
Solomon 13.115
Lehninger 13.013
Nozaki 12.618
DTASelect 12.603
Thurlkill 12.618
EMBOSS 13.115
Sillero 12.618
Patrickios 12.135
IPC_peptide 13.115
IPC2_peptide 12.091
IPC2.peptide.svr19 9.013
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1434
0
1434
450018
39
3245
313.8
35.16
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.341 ± 0.131
0.446 ± 0.014
5.896 ± 0.071
5.966 ± 0.069
4.278 ± 0.064
6.401 ± 0.058
1.983 ± 0.033
7.648 ± 0.077
7.89 ± 0.067
9.367 ± 0.113
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.445 ± 0.036
5.768 ± 0.064
3.661 ± 0.05
4.105 ± 0.051
3.526 ± 0.055
6.015 ± 0.121
5.852 ± 0.089
6.62 ± 0.055
1.012 ± 0.025
3.727 ± 0.045
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here