Amino acid dipepetide frequency for Lactobacillus rodentium

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.36AlaAla: 6.36 ± 0.366
0.444AlaCys: 0.444 ± 0.035
4.264AlaAsp: 4.264 ± 0.148
3.835AlaGlu: 3.835 ± 0.104
2.804AlaPhe: 2.804 ± 0.097
5.011AlaGly: 5.011 ± 0.119
1.396AlaHis: 1.396 ± 0.058
5.604AlaIle: 5.604 ± 0.128
5.855AlaLys: 5.855 ± 0.144
6.849AlaLeu: 6.849 ± 0.156
1.74AlaMet: 1.74 ± 0.075
3.858AlaAsn: 3.858 ± 0.116
2.253AlaPro: 2.253 ± 0.077
3.473AlaGln: 3.473 ± 0.115
2.442AlaArg: 2.442 ± 0.083
5.149AlaSer: 5.149 ± 0.515
4.36AlaThr: 4.36 ± 0.125
4.526AlaVal: 4.526 ± 0.113
0.687AlaTrp: 0.687 ± 0.041
2.495AlaTyr: 2.495 ± 0.071
0.002AlaXaa: 0.002 ± 0.002
Cys
0.28CysAla: 0.28 ± 0.028
0.038CysCys: 0.038 ± 0.008
0.276CysAsp: 0.276 ± 0.026
0.198CysGlu: 0.198 ± 0.022
0.216CysPhe: 0.216 ± 0.024
0.498CysGly: 0.498 ± 0.042
0.104CysHis: 0.104 ± 0.016
0.329CysIle: 0.329 ± 0.033
0.211CysLys: 0.211 ± 0.023
0.489CysLeu: 0.489 ± 0.038
0.111CysMet: 0.111 ± 0.015
0.176CysAsn: 0.176 ± 0.023
0.216CysPro: 0.216 ± 0.025
0.16CysGln: 0.16 ± 0.02
0.136CysArg: 0.136 ± 0.017
0.293CysSer: 0.293 ± 0.026
0.236CysThr: 0.236 ± 0.026
0.273CysVal: 0.273 ± 0.023
0.044CysTrp: 0.044 ± 0.009
0.178CysTyr: 0.178 ± 0.023
0.0CysXaa: 0.0 ± 0.0
Asp
3.882AspAla: 3.882 ± 0.127
0.182AspCys: 0.182 ± 0.022
3.3AspAsp: 3.3 ± 0.11
4.162AspGlu: 4.162 ± 0.11
2.664AspPhe: 2.664 ± 0.091
3.733AspGly: 3.733 ± 0.135
1.273AspHis: 1.273 ± 0.054
4.044AspIle: 4.044 ± 0.108
4.738AspLys: 4.738 ± 0.12
5.98AspLeu: 5.98 ± 0.125
1.444AspMet: 1.444 ± 0.062
3.067AspAsn: 3.067 ± 0.087
2.62AspPro: 2.62 ± 0.07
2.929AspGln: 2.929 ± 0.098
2.047AspArg: 2.047 ± 0.072
3.233AspSer: 3.233 ± 0.112
2.878AspThr: 2.878 ± 0.095
3.551AspVal: 3.551 ± 0.111
0.676AspTrp: 0.676 ± 0.039
2.56AspTyr: 2.56 ± 0.077
0.0AspXaa: 0.0 ± 0.0
Glu
4.458GluAla: 4.458 ± 0.121
0.198GluCys: 0.198 ± 0.022
3.671GluAsp: 3.671 ± 0.116
4.042GluGlu: 4.042 ± 0.117
2.295GluPhe: 2.295 ± 0.079
3.062GluGly: 3.062 ± 0.083
1.162GluHis: 1.162 ± 0.048
4.618GluIle: 4.618 ± 0.118
5.92GluLys: 5.92 ± 0.139
5.853GluLeu: 5.853 ± 0.127
1.622GluMet: 1.622 ± 0.069
3.822GluAsn: 3.822 ± 0.118
1.764GluPro: 1.764 ± 0.073
2.375GluGln: 2.375 ± 0.074
2.273GluArg: 2.273 ± 0.092
2.733GluSer: 2.733 ± 0.085
3.093GluThr: 3.093 ± 0.086
4.264GluVal: 4.264 ± 0.112
0.484GluTrp: 0.484 ± 0.03
1.944GluTyr: 1.944 ± 0.069
0.0GluXaa: 0.0 ± 0.0
Phe
3.182PheAla: 3.182 ± 0.098
0.26PheCys: 0.26 ± 0.022
2.787PheAsp: 2.787 ± 0.085
2.307PheGlu: 2.307 ± 0.073
2.209PhePhe: 2.209 ± 0.08
3.149PheGly: 3.149 ± 0.108
0.764PheHis: 0.764 ± 0.048
3.389PheIle: 3.389 ± 0.107
2.955PheLys: 2.955 ± 0.078
3.962PheLeu: 3.962 ± 0.116
1.031PheMet: 1.031 ± 0.055
2.482PheAsn: 2.482 ± 0.088
1.382PhePro: 1.382 ± 0.053
1.242PheGln: 1.242 ± 0.053
1.178PheArg: 1.178 ± 0.058
2.927PheSer: 2.927 ± 0.096
2.547PheThr: 2.547 ± 0.09
2.738PheVal: 2.738 ± 0.088
0.547PheTrp: 0.547 ± 0.039
1.749PheTyr: 1.749 ± 0.069
0.0PheXaa: 0.0 ± 0.0
Gly
4.455GlyAla: 4.455 ± 0.106
0.293GlyCys: 0.293 ± 0.029
3.364GlyAsp: 3.364 ± 0.116
3.555GlyGlu: 3.555 ± 0.108
2.84GlyPhe: 2.84 ± 0.083
3.893GlyGly: 3.893 ± 0.11
1.447GlyHis: 1.447 ± 0.06
5.64GlyIle: 5.64 ± 0.136
5.471GlyLys: 5.471 ± 0.129
6.089GlyLeu: 6.089 ± 0.117
1.773GlyMet: 1.773 ± 0.071
3.158GlyAsn: 3.158 ± 0.098
1.595GlyPro: 1.595 ± 0.059
2.742GlyGln: 2.742 ± 0.086
2.371GlyArg: 2.371 ± 0.085
3.835GlySer: 3.835 ± 0.106
3.693GlyThr: 3.693 ± 0.123
4.335GlyVal: 4.335 ± 0.102
0.644GlyTrp: 0.644 ± 0.04
2.813GlyTyr: 2.813 ± 0.09
0.0GlyXaa: 0.0 ± 0.0
His
1.433HisAla: 1.433 ± 0.059
0.08HisCys: 0.08 ± 0.014
1.089HisAsp: 1.089 ± 0.049
1.302HisGlu: 1.302 ± 0.057
1.042HisPhe: 1.042 ± 0.051
1.373HisGly: 1.373 ± 0.061
0.642HisHis: 0.642 ± 0.038
1.224HisIle: 1.224 ± 0.057
1.162HisLys: 1.162 ± 0.053
1.967HisLeu: 1.967 ± 0.07
0.484HisMet: 0.484 ± 0.032
1.051HisAsn: 1.051 ± 0.052
1.044HisPro: 1.044 ± 0.048
0.904HisGln: 0.904 ± 0.046
0.78HisArg: 0.78 ± 0.042
1.009HisSer: 1.009 ± 0.052
1.027HisThr: 1.027 ± 0.049
1.167HisVal: 1.167 ± 0.049
0.191HisTrp: 0.191 ± 0.022
0.86HisTyr: 0.86 ± 0.044
0.0HisXaa: 0.0 ± 0.0
Ile
5.949IleAla: 5.949 ± 0.124
0.509IleCys: 0.509 ± 0.04
4.582IleAsp: 4.582 ± 0.102
3.955IleGlu: 3.955 ± 0.112
3.842IlePhe: 3.842 ± 0.128
5.229IleGly: 5.229 ± 0.138
1.202IleHis: 1.202 ± 0.05
6.38IleIle: 6.38 ± 0.157
5.773IleLys: 5.773 ± 0.117
7.098IleLeu: 7.098 ± 0.168
1.667IleMet: 1.667 ± 0.063
4.413IleAsn: 4.413 ± 0.102
3.14IlePro: 3.14 ± 0.103
2.375IleGln: 2.375 ± 0.081
2.32IleArg: 2.32 ± 0.082
5.026IleSer: 5.026 ± 0.114
4.451IleThr: 4.451 ± 0.121
5.178IleVal: 5.178 ± 0.129
0.756IleTrp: 0.756 ± 0.041
2.64IleTyr: 2.64 ± 0.082
0.0IleXaa: 0.0 ± 0.0
Lys
5.066LysAla: 5.066 ± 0.14
0.213LysCys: 0.213 ± 0.023
5.086LysAsp: 5.086 ± 0.125
5.871LysGlu: 5.871 ± 0.138
2.642LysPhe: 2.642 ± 0.078
3.922LysGly: 3.922 ± 0.112
1.422LysHis: 1.422 ± 0.061
6.018LysIle: 6.018 ± 0.127
7.533LysLys: 7.533 ± 0.171
6.731LysLeu: 6.731 ± 0.129
2.409LysMet: 2.409 ± 0.071
5.164LysAsn: 5.164 ± 0.109
2.567LysPro: 2.567 ± 0.083
3.458LysGln: 3.458 ± 0.099
3.295LysArg: 3.295 ± 0.105
3.969LysSer: 3.969 ± 0.119
4.327LysThr: 4.327 ± 0.117
5.28LysVal: 5.28 ± 0.111
0.722LysTrp: 0.722 ± 0.042
3.209LysTyr: 3.209 ± 0.086
0.0LysXaa: 0.0 ± 0.0
Leu
7.757LeuAla: 7.757 ± 0.164
0.462LeuCys: 0.462 ± 0.036
5.413LeuAsp: 5.413 ± 0.126
4.931LeuGlu: 4.931 ± 0.118
3.935LeuPhe: 3.935 ± 0.135
6.2LeuGly: 6.2 ± 0.142
1.764LeuHis: 1.764 ± 0.071
7.429LeuIle: 7.429 ± 0.18
7.135LeuLys: 7.135 ± 0.137
8.651LeuLeu: 8.651 ± 0.249
2.211LeuMet: 2.211 ± 0.078
5.562LeuAsn: 5.562 ± 0.124
3.913LeuPro: 3.913 ± 0.103
3.16LeuGln: 3.16 ± 0.104
3.247LeuArg: 3.247 ± 0.088
5.895LeuSer: 5.895 ± 0.12
6.2LeuThr: 6.2 ± 0.128
6.175LeuVal: 6.175 ± 0.129
0.802LeuTrp: 0.802 ± 0.047
2.827LeuTyr: 2.827 ± 0.08
0.0LeuXaa: 0.0 ± 0.0
Met
2.038MetAla: 2.038 ± 0.079
0.113MetCys: 0.113 ± 0.015
1.367MetAsp: 1.367 ± 0.061
1.24MetGlu: 1.24 ± 0.058
0.849MetPhe: 0.849 ± 0.043
1.535MetGly: 1.535 ± 0.069
0.478MetHis: 0.478 ± 0.03
1.982MetIle: 1.982 ± 0.074
2.089MetLys: 2.089 ± 0.069
2.069MetLeu: 2.069 ± 0.077
0.72MetMet: 0.72 ± 0.039
1.413MetAsn: 1.413 ± 0.05
1.071MetPro: 1.071 ± 0.05
1.004MetGln: 1.004 ± 0.048
0.88MetArg: 0.88 ± 0.047
1.611MetSer: 1.611 ± 0.058
1.611MetThr: 1.611 ± 0.065
1.56MetVal: 1.56 ± 0.065
0.173MetTrp: 0.173 ± 0.023
0.642MetTyr: 0.642 ± 0.034
0.0MetXaa: 0.0 ± 0.0
Asn
3.442AsnAla: 3.442 ± 0.123
0.278AsnCys: 0.278 ± 0.028
3.22AsnAsp: 3.22 ± 0.086
3.449AsnGlu: 3.449 ± 0.084
2.535AsnPhe: 2.535 ± 0.08
3.958AsnGly: 3.958 ± 0.121
1.289AsnHis: 1.289 ± 0.057
4.002AsnIle: 4.002 ± 0.09
4.713AsnLys: 4.713 ± 0.126
5.333AsnLeu: 5.333 ± 0.122
1.396AsnMet: 1.396 ± 0.062
3.853AsnAsn: 3.853 ± 0.116
2.564AsnPro: 2.564 ± 0.095
2.675AsnGln: 2.675 ± 0.087
1.895AsnArg: 1.895 ± 0.072
3.578AsnSer: 3.578 ± 0.115
2.824AsnThr: 2.824 ± 0.098
3.362AsnVal: 3.362 ± 0.127
0.738AsnTrp: 0.738 ± 0.042
2.575AsnTyr: 2.575 ± 0.084
0.0AsnXaa: 0.0 ± 0.0
Pro
2.653ProAla: 2.653 ± 0.078
0.078ProCys: 0.078 ± 0.013
2.584ProAsp: 2.584 ± 0.095
3.109ProGlu: 3.109 ± 0.101
1.587ProPhe: 1.587 ± 0.058
2.131ProGly: 2.131 ± 0.081
0.776ProHis: 0.776 ± 0.04
2.613ProIle: 2.613 ± 0.073
2.655ProLys: 2.655 ± 0.077
3.024ProLeu: 3.024 ± 0.09
0.716ProMet: 0.716 ± 0.041
2.202ProAsn: 2.202 ± 0.087
0.618ProPro: 0.618 ± 0.039
1.573ProGln: 1.573 ± 0.051
1.093ProArg: 1.093 ± 0.051
2.049ProSer: 2.049 ± 0.076
2.587ProThr: 2.587 ± 0.113
2.831ProVal: 2.831 ± 0.075
0.349ProTrp: 0.349 ± 0.028
1.38ProTyr: 1.38 ± 0.053
0.002ProXaa: 0.002 ± 0.003
Gln
3.302GlnAla: 3.302 ± 0.11
0.08GlnCys: 0.08 ± 0.013
2.278GlnAsp: 2.278 ± 0.081
2.782GlnGlu: 2.782 ± 0.089
1.422GlnPhe: 1.422 ± 0.06
2.144GlnGly: 2.144 ± 0.069
0.649GlnHis: 0.649 ± 0.033
3.278GlnIle: 3.278 ± 0.092
3.404GlnLys: 3.404 ± 0.097
3.907GlnLeu: 3.907 ± 0.115
1.169GlnMet: 1.169 ± 0.058
2.455GlnAsn: 2.455 ± 0.079
1.358GlnPro: 1.358 ± 0.057
1.724GlnGln: 1.724 ± 0.077
1.644GlnArg: 1.644 ± 0.064
1.96GlnSer: 1.96 ± 0.067
2.72GlnThr: 2.72 ± 0.097
2.913GlnVal: 2.913 ± 0.082
0.429GlnTrp: 0.429 ± 0.032
1.436GlnTyr: 1.436 ± 0.06
0.0GlnXaa: 0.0 ± 0.0
Arg
2.347ArgAla: 2.347 ± 0.089
0.138ArgCys: 0.138 ± 0.021
1.947ArgAsp: 1.947 ± 0.073
2.22ArgGlu: 2.22 ± 0.083
1.571ArgPhe: 1.571 ± 0.055
2.002ArgGly: 2.002 ± 0.074
0.824ArgHis: 0.824 ± 0.049
2.831ArgIle: 2.831 ± 0.084
2.862ArgLys: 2.862 ± 0.101
3.4ArgLeu: 3.4 ± 0.097
0.913ArgMet: 0.913 ± 0.044
1.893ArgAsn: 1.893 ± 0.064
1.311ArgPro: 1.311 ± 0.063
1.622ArgGln: 1.622 ± 0.058
1.787ArgArg: 1.787 ± 0.072
1.853ArgSer: 1.853 ± 0.071
1.755ArgThr: 1.755 ± 0.067
2.318ArgVal: 2.318 ± 0.078
0.32ArgTrp: 0.32 ± 0.028
1.344ArgTyr: 1.344 ± 0.059
0.0ArgXaa: 0.0 ± 0.0
Ser
4.44SerAla: 4.44 ± 0.372
0.229SerCys: 0.229 ± 0.022
3.569SerAsp: 3.569 ± 0.132
3.467SerGlu: 3.467 ± 0.119
2.713SerPhe: 2.713 ± 0.077
4.262SerGly: 4.262 ± 0.102
1.267SerHis: 1.267 ± 0.048
4.084SerIle: 4.084 ± 0.103
4.367SerLys: 4.367 ± 0.101
5.695SerLeu: 5.695 ± 0.121
1.28SerMet: 1.28 ± 0.058
3.411SerAsn: 3.411 ± 0.12
1.769SerPro: 1.769 ± 0.067
2.602SerGln: 2.602 ± 0.095
2.075SerArg: 2.075 ± 0.067
4.649SerSer: 4.649 ± 0.327
3.382SerThr: 3.382 ± 0.134
3.789SerVal: 3.789 ± 0.095
0.829SerTrp: 0.829 ± 0.048
2.271SerTyr: 2.271 ± 0.079
0.0SerXaa: 0.0 ± 0.0
Thr
4.304ThrAla: 4.304 ± 0.132
0.251ThrCys: 0.251 ± 0.028
3.282ThrAsp: 3.282 ± 0.115
2.991ThrGlu: 2.991 ± 0.078
2.578ThrPhe: 2.578 ± 0.076
4.195ThrGly: 4.195 ± 0.108
1.004ThrHis: 1.004 ± 0.051
4.675ThrIle: 4.675 ± 0.118
4.107ThrLys: 4.107 ± 0.128
5.382ThrLeu: 5.382 ± 0.126
1.138ThrMet: 1.138 ± 0.054
3.4ThrAsn: 3.4 ± 0.12
2.829ThrPro: 2.829 ± 0.125
2.273ThrGln: 2.273 ± 0.086
1.72ThrArg: 1.72 ± 0.067
3.64ThrSer: 3.64 ± 0.134
3.809ThrThr: 3.809 ± 0.138
4.155ThrVal: 4.155 ± 0.129
0.578ThrTrp: 0.578 ± 0.043
2.209ThrTyr: 2.209 ± 0.075
0.0ThrXaa: 0.0 ± 0.0
Val
5.111ValAla: 5.111 ± 0.141
0.349ValCys: 0.349 ± 0.031
3.978ValAsp: 3.978 ± 0.111
3.729ValGlu: 3.729 ± 0.103
2.52ValPhe: 2.52 ± 0.088
4.573ValGly: 4.573 ± 0.113
1.118ValHis: 1.118 ± 0.05
5.206ValIle: 5.206 ± 0.101
5.262ValLys: 5.262 ± 0.125
5.878ValLeu: 5.878 ± 0.127
1.578ValMet: 1.578 ± 0.065
3.669ValAsn: 3.669 ± 0.106
2.762ValPro: 2.762 ± 0.099
2.278ValGln: 2.278 ± 0.07
2.135ValArg: 2.135 ± 0.081
4.058ValSer: 4.058 ± 0.107
4.5ValThr: 4.5 ± 0.19
4.818ValVal: 4.818 ± 0.118
0.578ValTrp: 0.578 ± 0.036
2.104ValTyr: 2.104 ± 0.071
0.002ValXaa: 0.002 ± 0.002
Trp
0.667TrpAla: 0.667 ± 0.04
0.069TrpCys: 0.069 ± 0.013
0.56TrpAsp: 0.56 ± 0.036
0.427TrpGlu: 0.427 ± 0.034
0.553TrpPhe: 0.553 ± 0.039
0.613TrpGly: 0.613 ± 0.04
0.278TrpHis: 0.278 ± 0.023
0.78TrpIle: 0.78 ± 0.048
0.644TrpLys: 0.644 ± 0.044
1.156TrpLeu: 1.156 ± 0.058
0.262TrpMet: 0.262 ± 0.025
0.676TrpAsn: 0.676 ± 0.048
0.316TrpPro: 0.316 ± 0.027
0.52TrpGln: 0.52 ± 0.032
0.378TrpArg: 0.378 ± 0.027
0.54TrpSer: 0.54 ± 0.034
0.462TrpThr: 0.462 ± 0.037
0.633TrpVal: 0.633 ± 0.047
0.149TrpTrp: 0.149 ± 0.019
0.436TrpTyr: 0.436 ± 0.035
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.282TyrAla: 2.282 ± 0.074
0.196TyrCys: 0.196 ± 0.023
2.347TyrAsp: 2.347 ± 0.07
2.084TyrGlu: 2.084 ± 0.078
1.967TyrPhe: 1.967 ± 0.072
2.524TyrGly: 2.524 ± 0.078
0.973TyrHis: 0.973 ± 0.05
2.358TyrIle: 2.358 ± 0.078
2.044TyrLys: 2.044 ± 0.072
4.16TyrLeu: 4.16 ± 0.1
0.78TyrMet: 0.78 ± 0.042
1.955TyrAsn: 1.955 ± 0.059
1.518TyrPro: 1.518 ± 0.073
1.962TyrGln: 1.962 ± 0.08
1.562TyrArg: 1.562 ± 0.068
2.144TyrSer: 2.144 ± 0.076
2.06TyrThr: 2.06 ± 0.085
2.338TyrVal: 2.338 ± 0.064
0.422TyrTrp: 0.422 ± 0.033
1.593TyrTyr: 1.593 ± 0.069
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.002XaaLys: 0.002 ± 0.002
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.004XaaTyr: 0.004 ± 0.003
0.516XaaXaa: 0.516 ± 0.298
Statistics based on 1434 proteins (450018 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski