Alces alces faeces associated genomovirus MP111
Average proteome isoelectric point is 7.13
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A2Z5CL71|A0A2Z5CL71_9VIRU Capsid protein OS=Alces alces faeces associated genomovirus MP111 OX=2219111 PE=4 SV=1
MM1 pKa = 7.71 PFSFNARR8 pKa = 11.84 YY9 pKa = 9.01 FLVTYY14 pKa = 9.29 SHH16 pKa = 7.06 VDD18 pKa = 3.21 EE19 pKa = 5.72 LDD21 pKa = 3.3 PFAIVDD27 pKa = 3.62 HH28 pKa = 6.61 FGSLGAEE35 pKa = 3.98 CIVARR40 pKa = 11.84 EE41 pKa = 4.11 RR42 pKa = 11.84 YY43 pKa = 9.22 ADD45 pKa = 3.72 LLGVHH50 pKa = 5.72 YY51 pKa = 10.48 HH52 pKa = 5.67 VFADD56 pKa = 4.64 FGRR59 pKa = 11.84 KK60 pKa = 8.21 FRR62 pKa = 11.84 SRR64 pKa = 11.84 RR65 pKa = 11.84 ANVFDD70 pKa = 3.5 VGEE73 pKa = 4.04 FHH75 pKa = 7.54 PNISPSRR82 pKa = 11.84 GTPEE86 pKa = 4.62 AGFDD90 pKa = 3.64 YY91 pKa = 9.31 ATKK94 pKa = 10.77 DD95 pKa = 3.16 GDD97 pKa = 3.83 IVGGGLEE104 pKa = 4.05 RR105 pKa = 11.84 PSRR108 pKa = 11.84 DD109 pKa = 3.09 GALSRR114 pKa = 11.84 SAKK117 pKa = 8.28 WAQIVNAEE125 pKa = 4.27 SRR127 pKa = 11.84 DD128 pKa = 3.5 EE129 pKa = 4.36 FYY131 pKa = 10.98 RR132 pKa = 11.84 LCEE135 pKa = 3.91 EE136 pKa = 5.02 LDD138 pKa = 3.68 PEE140 pKa = 4.71 RR141 pKa = 11.84 LVCSFGQIQKK151 pKa = 10.02 FADD154 pKa = 3.08 WKK156 pKa = 10.52 FAVTPAPYY164 pKa = 8.43 ATPDD168 pKa = 3.53 GVFDD172 pKa = 4.28 LANYY176 pKa = 9.84 GDD178 pKa = 4.11 LGEE181 pKa = 4.48 SKK183 pKa = 10.86 SLILYY188 pKa = 8.74 GPSRR192 pKa = 11.84 LGKK195 pKa = 7.29 TVWSRR200 pKa = 11.84 SLGEE204 pKa = 3.86 HH205 pKa = 6.9 FYY207 pKa = 11.23 FGGIFSARR215 pKa = 11.84 DD216 pKa = 3.36 LDD218 pKa = 4.32 RR219 pKa = 11.84 DD220 pKa = 3.87 DD221 pKa = 3.88 VKK223 pKa = 11.41 YY224 pKa = 11.03 AVFDD228 pKa = 5.22 DD229 pKa = 3.44 IAGGIKK235 pKa = 10.03 FFPRR239 pKa = 11.84 FKK241 pKa = 10.79 DD242 pKa = 3.12 WLGCQMEE249 pKa = 4.46 FMVKK253 pKa = 9.27 QLYY256 pKa = 9.95 RR257 pKa = 11.84 DD258 pKa = 3.6 PQLFRR263 pKa = 11.84 WGRR266 pKa = 11.84 PCIWVANTDD275 pKa = 3.19 PRR277 pKa = 11.84 LDD279 pKa = 3.67 MTHH282 pKa = 7.34 DD283 pKa = 4.81 DD284 pKa = 4.55 IMWLEE289 pKa = 4.07 ANCIFVEE296 pKa = 3.88 ITEE299 pKa = 5.25 AIFHH303 pKa = 6.92 ANTEE307 pKa = 4.17
Molecular weight: 35.08 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.998
IPC2_protein 5.041
IPC_protein 5.003
Toseland 4.952
ProMoST 5.105
Dawson 5.029
Bjellqvist 5.169
Wikipedia 4.94
Rodwell 4.927
Grimsley 4.889
Solomon 5.029
Lehninger 4.991
Nozaki 5.156
DTASelect 5.359
Thurlkill 4.978
EMBOSS 4.991
Sillero 5.219
Patrickios 3.948
IPC_peptide 5.029
IPC2_peptide 5.207
IPC2.peptide.svr19 5.155
Protein with the highest isoelectric point:
>tr|A0A2Z5CL71|A0A2Z5CL71_9VIRU Capsid protein OS=Alces alces faeces associated genomovirus MP111 OX=2219111 PE=4 SV=1
MM1 pKa = 7.34 ARR3 pKa = 11.84 TWASIYY9 pKa = 10.05 RR10 pKa = 11.84 KK11 pKa = 8.88 PFRR14 pKa = 11.84 RR15 pKa = 11.84 TRR17 pKa = 11.84 PVRR20 pKa = 11.84 GGRR23 pKa = 11.84 TTRR26 pKa = 11.84 RR27 pKa = 11.84 STKK30 pKa = 9.79 KK31 pKa = 9.68 RR32 pKa = 11.84 PTYY35 pKa = 9.62 RR36 pKa = 11.84 RR37 pKa = 11.84 KK38 pKa = 10.23 SRR40 pKa = 11.84 TMSKK44 pKa = 10.37 KK45 pKa = 10.11 RR46 pKa = 11.84 ILNISSRR53 pKa = 11.84 KK54 pKa = 9.6 KK55 pKa = 9.61 RR56 pKa = 11.84 DD57 pKa = 2.93 TMAPIQQNGSSGTPAIGALNINGAPGNPGNATFVFWCATARR98 pKa = 11.84 DD99 pKa = 3.88 NTVAQGGAASSVYY112 pKa = 10.56 NPEE115 pKa = 3.46 TRR117 pKa = 11.84 TATTCYY123 pKa = 9.78 MRR125 pKa = 11.84 GLKK128 pKa = 10.33 EE129 pKa = 3.93 NVEE132 pKa = 4.48 MQTSTGIPWQWRR144 pKa = 11.84 RR145 pKa = 11.84 ICFTYY150 pKa = 11.02 KK151 pKa = 11.0 DD152 pKa = 3.83 MTEE155 pKa = 3.89 VCATNNGGISGFYY168 pKa = 9.85 EE169 pKa = 4.29 GSNGYY174 pKa = 9.05 GRR176 pKa = 11.84 LMYY179 pKa = 11.25 NMFSSGSAADD189 pKa = 3.65 VKK191 pKa = 10.97 AYY193 pKa = 7.81 TALQTLLFRR202 pKa = 11.84 GTIGVDD208 pKa = 2.58 WNDD211 pKa = 4.22 YY212 pKa = 8.15 ITAPIDD218 pKa = 3.54 TQRR221 pKa = 11.84 VGLKK225 pKa = 9.45 YY226 pKa = 10.83 DD227 pKa = 3.31 KK228 pKa = 10.94 TRR230 pKa = 11.84 VIQSGNANGMLRR242 pKa = 11.84 KK243 pKa = 9.46 YY244 pKa = 10.35 QMWHH248 pKa = 6.4 PMNKK252 pKa = 9.54 NLVYY256 pKa = 10.62 DD257 pKa = 4.29 DD258 pKa = 4.47 SEE260 pKa = 5.04 KK261 pKa = 11.28 GGADD265 pKa = 2.99 TAGYY269 pKa = 9.69 FSIGGKK275 pKa = 9.49 PGMGDD280 pKa = 3.73 FLVLDD285 pKa = 3.86 IFMPGKK291 pKa = 10.31 GSSSSDD297 pKa = 3.27 VLSFEE302 pKa = 4.53 ATSTLYY308 pKa = 9.64 WHH310 pKa = 7.08 EE311 pKa = 4.2 KK312 pKa = 9.32
Molecular weight: 34.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.27
IPC2_protein 9.589
IPC_protein 9.867
Toseland 10.131
ProMoST 9.897
Dawson 10.35
Bjellqvist 10.058
Wikipedia 10.54
Rodwell 10.687
Grimsley 10.438
Solomon 10.379
Lehninger 10.335
Nozaki 10.145
DTASelect 10.043
Thurlkill 10.189
EMBOSS 10.54
Sillero 10.262
Patrickios 10.175
IPC_peptide 10.379
IPC2_peptide 8.887
IPC2.peptide.svr19 8.583
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2
0
2
619
307
312
309.5
34.88
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.754 ± 0.291
1.616 ± 0.254
6.785 ± 1.748
4.2 ± 1.245
6.139 ± 1.988
9.37 ± 0.918
1.616 ± 0.741
4.847 ± 0.03
4.847 ± 0.946
5.816 ± 1.011
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.908 ± 0.958
4.2 ± 1.193
4.523 ± 0.271
2.423 ± 0.351
7.916 ± 0.317
6.785 ± 0.934
6.462 ± 2.399
5.17 ± 0.763
2.262 ± 0.014
4.362 ± 0.339
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here