Mycobacterium phage DyoEdafos

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; Bronvirus; unclassified Bronvirus

Average proteome isoelectric point is 6.28

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 142 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A5J6TIY7|A0A5J6TIY7_9CAUD Uncharacterized protein OS=Mycobacterium phage DyoEdafos OX=2599860 GN=148 PE=4 SV=1
MM1 pKa = 7.53APLYY5 pKa = 10.81DD6 pKa = 3.92RR7 pKa = 11.84LNSVALSLHH16 pKa = 6.3FGCDD20 pKa = 3.52EE21 pKa = 4.11EE22 pKa = 4.55STAAKK27 pKa = 9.66SQQDD31 pKa = 3.92IAEE34 pKa = 4.07MAYY37 pKa = 10.51DD38 pKa = 4.28VIRR41 pKa = 11.84DD42 pKa = 3.34LWGYY46 pKa = 10.46DD47 pKa = 3.28VSDD50 pKa = 3.23EE51 pKa = 4.22HH52 pKa = 8.72AFIKK56 pKa = 10.79AILDD60 pKa = 3.59TFDD63 pKa = 3.03MYY65 pKa = 11.19GYY67 pKa = 9.85PDD69 pKa = 4.16LEE71 pKa = 4.26SLKK74 pKa = 10.91GISEE78 pKa = 4.1MSALDD83 pKa = 3.43MADD86 pKa = 4.39YY87 pKa = 10.64ILQLLPTDD95 pKa = 3.49ALTPKK100 pKa = 10.59RR101 pKa = 11.84SDD103 pKa = 3.67TLCAAPLL110 pKa = 3.6

Molecular weight: 12.23 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A5J6TKK0|A0A5J6TKK0_9CAUD Uncharacterized protein OS=Mycobacterium phage DyoEdafos OX=2599860 GN=60 PE=4 SV=1
MM1 pKa = 7.27IHH3 pKa = 6.04QLAHH7 pKa = 4.99VAAVVPVEE15 pKa = 4.43DD16 pKa = 3.75RR17 pKa = 11.84ALMFLLVVTTVITQAIRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84TWHH39 pKa = 5.79VRR41 pKa = 11.84WEE43 pKa = 4.24TGTTLTGALMCAALILLSPNLVAFNPFLPDD73 pKa = 3.2ALEE76 pKa = 4.18SGRR79 pKa = 11.84VYY81 pKa = 9.81PQVMAGYY88 pKa = 9.23LVLMLAYY95 pKa = 8.87GTFLYY100 pKa = 10.81DD101 pKa = 3.89LAGRR105 pKa = 11.84MDD107 pKa = 3.38WDD109 pKa = 3.66EE110 pKa = 4.33SQKK113 pKa = 10.35HH114 pKa = 4.97RR115 pKa = 11.84YY116 pKa = 5.57ITRR119 pKa = 11.84RR120 pKa = 11.84ISLPTTIALPTAVGAWITGSHH141 pKa = 5.32WLVFPMVLIGYY152 pKa = 8.17VWLLSHH158 pKa = 6.98ICWLLWCIRR167 pKa = 11.84TSDD170 pKa = 3.67RR171 pKa = 11.84RR172 pKa = 11.84SHH174 pKa = 6.43LVIDD178 pKa = 4.29VYY180 pKa = 10.8MVAFALCGLAWSSRR194 pKa = 11.84YY195 pKa = 9.14IVSLSITTNWGWRR208 pKa = 11.84LATVGAILLMVGSTLSWRR226 pKa = 11.84RR227 pKa = 11.84KK228 pKa = 7.93LRR230 pKa = 11.84YY231 pKa = 8.62MKK233 pKa = 10.55AHH235 pKa = 6.36MWRR238 pKa = 11.84AVRR241 pKa = 11.84RR242 pKa = 11.84QKK244 pKa = 10.21PLKK247 pKa = 10.24RR248 pKa = 11.84NAKK251 pKa = 8.34ATAPKK256 pKa = 10.34QLDD259 pKa = 3.78GAVV262 pKa = 3.17

Molecular weight: 29.82 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

142

0

142

23525

25

1697

165.7

18.23

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.487 ± 0.301

1.041 ± 0.116

6.767 ± 0.173

5.709 ± 0.245

3.116 ± 0.115

8.425 ± 0.376

1.964 ± 0.142

4.174 ± 0.137

5.02 ± 0.195

8.429 ± 0.247

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.436 ± 0.11

3.745 ± 0.177

5.016 ± 0.192

3.596 ± 0.155

6.138 ± 0.265

5.696 ± 0.202

5.624 ± 0.201

7.481 ± 0.222

2.193 ± 0.16

2.942 ± 0.166
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski