Amino acid dipepetide frequency for Mycobacterium phage DyoEdafos

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.455AlaAla: 12.455 ± 0.803
0.808AlaCys: 0.808 ± 0.196
7.354AlaAsp: 7.354 ± 0.445
6.802AlaGlu: 6.802 ± 0.618
3.018AlaPhe: 3.018 ± 0.285
8.077AlaGly: 8.077 ± 0.744
1.7AlaHis: 1.7 ± 0.291
4.464AlaIle: 4.464 ± 0.435
5.951AlaLys: 5.951 ± 0.52
9.905AlaLeu: 9.905 ± 0.576
3.188AlaMet: 3.188 ± 0.345
3.826AlaAsn: 3.826 ± 0.471
4.081AlaPro: 4.081 ± 0.494
4.123AlaGln: 4.123 ± 0.525
5.441AlaArg: 5.441 ± 0.439
6.206AlaSer: 6.206 ± 0.781
5.696AlaThr: 5.696 ± 0.477
7.354AlaVal: 7.354 ± 0.702
1.615AlaTrp: 1.615 ± 0.288
2.806AlaTyr: 2.806 ± 0.328
0.0AlaXaa: 0.0 ± 0.0
Cys
0.638CysAla: 0.638 ± 0.164
0.17CysCys: 0.17 ± 0.09
0.765CysAsp: 0.765 ± 0.174
0.68CysGlu: 0.68 ± 0.22
0.213CysPhe: 0.213 ± 0.101
1.148CysGly: 1.148 ± 0.218
0.383CysHis: 0.383 ± 0.119
0.34CysIle: 0.34 ± 0.12
0.383CysLys: 0.383 ± 0.135
0.808CysLeu: 0.808 ± 0.183
0.425CysMet: 0.425 ± 0.128
0.425CysAsn: 0.425 ± 0.131
0.51CysPro: 0.51 ± 0.142
0.34CysGln: 0.34 ± 0.137
0.893CysArg: 0.893 ± 0.243
0.595CysSer: 0.595 ± 0.17
0.51CysThr: 0.51 ± 0.151
0.85CysVal: 0.85 ± 0.199
0.128CysTrp: 0.128 ± 0.066
0.213CysTyr: 0.213 ± 0.099
0.0CysXaa: 0.0 ± 0.0
Asp
6.334AspAla: 6.334 ± 0.525
0.978AspCys: 0.978 ± 0.247
4.761AspAsp: 4.761 ± 0.518
4.634AspGlu: 4.634 ± 0.523
2.381AspPhe: 2.381 ± 0.266
6.291AspGly: 6.291 ± 0.498
1.19AspHis: 1.19 ± 0.302
3.146AspIle: 3.146 ± 0.354
3.571AspLys: 3.571 ± 0.421
5.696AspLeu: 5.696 ± 0.469
1.7AspMet: 1.7 ± 0.335
2.381AspAsn: 2.381 ± 0.374
3.273AspPro: 3.273 ± 0.433
1.785AspGln: 1.785 ± 0.277
3.741AspArg: 3.741 ± 0.408
3.571AspSer: 3.571 ± 0.379
3.613AspThr: 3.613 ± 0.384
4.379AspVal: 4.379 ± 0.465
1.913AspTrp: 1.913 ± 0.285
2.338AspTyr: 2.338 ± 0.382
0.0AspXaa: 0.0 ± 0.0
Glu
7.184GluAla: 7.184 ± 0.718
0.723GluCys: 0.723 ± 0.18
2.976GluAsp: 2.976 ± 0.34
2.423GluGlu: 2.423 ± 0.334
2.125GluPhe: 2.125 ± 0.326
3.571GluGly: 3.571 ± 0.407
1.573GluHis: 1.573 ± 0.221
2.593GluIle: 2.593 ± 0.363
2.211GluLys: 2.211 ± 0.351
7.354GluLeu: 7.354 ± 0.587
1.7GluMet: 1.7 ± 0.24
1.615GluAsn: 1.615 ± 0.291
2.381GluPro: 2.381 ± 0.426
2.296GluGln: 2.296 ± 0.338
4.166GluArg: 4.166 ± 0.576
3.018GluSer: 3.018 ± 0.333
2.933GluThr: 2.933 ± 0.322
3.273GluVal: 3.273 ± 0.385
1.02GluTrp: 1.02 ± 0.204
1.955GluTyr: 1.955 ± 0.303
0.0GluXaa: 0.0 ± 0.0
Phe
2.338PheAla: 2.338 ± 0.328
0.34PheCys: 0.34 ± 0.114
2.168PheAsp: 2.168 ± 0.373
1.7PheGlu: 1.7 ± 0.266
1.318PhePhe: 1.318 ± 0.208
2.721PheGly: 2.721 ± 0.336
0.723PheHis: 0.723 ± 0.186
1.403PheIle: 1.403 ± 0.239
1.615PheLys: 1.615 ± 0.269
2.551PheLeu: 2.551 ± 0.29
0.553PheMet: 0.553 ± 0.155
1.445PheAsn: 1.445 ± 0.231
1.998PhePro: 1.998 ± 0.251
0.808PheGln: 0.808 ± 0.209
1.828PheArg: 1.828 ± 0.287
1.573PheSer: 1.573 ± 0.255
1.955PheThr: 1.955 ± 0.276
2.253PheVal: 2.253 ± 0.284
0.425PheTrp: 0.425 ± 0.146
1.445PheTyr: 1.445 ± 0.251
0.0PheXaa: 0.0 ± 0.0
Gly
7.312GlyAla: 7.312 ± 0.697
0.935GlyCys: 0.935 ± 0.205
5.144GlyAsp: 5.144 ± 0.42
5.186GlyGlu: 5.186 ± 0.455
2.678GlyPhe: 2.678 ± 0.402
8.034GlyGly: 8.034 ± 0.982
1.743GlyHis: 1.743 ± 0.324
3.953GlyIle: 3.953 ± 0.277
4.421GlyLys: 4.421 ± 0.505
7.609GlyLeu: 7.609 ± 0.865
1.573GlyMet: 1.573 ± 0.238
2.466GlyAsn: 2.466 ± 0.272
3.401GlyPro: 3.401 ± 0.414
3.061GlyGln: 3.061 ± 0.326
4.846GlyArg: 4.846 ± 0.41
4.974GlySer: 4.974 ± 0.546
5.059GlyThr: 5.059 ± 0.63
6.589GlyVal: 6.589 ± 0.539
2.125GlyTrp: 2.125 ± 0.331
3.146GlyTyr: 3.146 ± 0.378
0.0GlyXaa: 0.0 ± 0.0
His
1.403HisAla: 1.403 ± 0.256
0.213HisCys: 0.213 ± 0.093
1.488HisAsp: 1.488 ± 0.269
1.233HisGlu: 1.233 ± 0.221
0.85HisPhe: 0.85 ± 0.178
1.998HisGly: 1.998 ± 0.321
0.34HisHis: 0.34 ± 0.152
1.19HisIle: 1.19 ± 0.234
0.68HisLys: 0.68 ± 0.149
1.403HisLeu: 1.403 ± 0.206
0.51HisMet: 0.51 ± 0.135
0.425HisAsn: 0.425 ± 0.147
1.063HisPro: 1.063 ± 0.216
0.723HisGln: 0.723 ± 0.168
1.233HisArg: 1.233 ± 0.252
0.85HisSer: 0.85 ± 0.214
0.978HisThr: 0.978 ± 0.17
1.615HisVal: 1.615 ± 0.265
0.51HisTrp: 0.51 ± 0.153
0.935HisTyr: 0.935 ± 0.216
0.0HisXaa: 0.0 ± 0.0
Ile
4.931IleAla: 4.931 ± 0.437
0.468IleCys: 0.468 ± 0.158
3.061IleAsp: 3.061 ± 0.414
2.933IleGlu: 2.933 ± 0.369
1.02IlePhe: 1.02 ± 0.212
2.976IleGly: 2.976 ± 0.414
0.723IleHis: 0.723 ± 0.218
1.488IleIle: 1.488 ± 0.241
1.955IleLys: 1.955 ± 0.313
3.103IleLeu: 3.103 ± 0.333
0.68IleMet: 0.68 ± 0.142
1.913IleAsn: 1.913 ± 0.255
2.253IlePro: 2.253 ± 0.293
1.615IleGln: 1.615 ± 0.283
3.061IleArg: 3.061 ± 0.318
2.508IleSer: 2.508 ± 0.384
2.466IleThr: 2.466 ± 0.316
3.103IleVal: 3.103 ± 0.328
0.723IleTrp: 0.723 ± 0.223
0.765IleTyr: 0.765 ± 0.153
0.0IleXaa: 0.0 ± 0.0
Lys
6.759LysAla: 6.759 ± 0.641
0.553LysCys: 0.553 ± 0.148
3.103LysAsp: 3.103 ± 0.4
2.551LysGlu: 2.551 ± 0.319
1.233LysPhe: 1.233 ± 0.226
3.826LysGly: 3.826 ± 0.545
1.063LysHis: 1.063 ± 0.234
1.828LysIle: 1.828 ± 0.309
2.763LysLys: 2.763 ± 0.363
3.698LysLeu: 3.698 ± 0.39
1.105LysMet: 1.105 ± 0.222
2.04LysAsn: 2.04 ± 0.297
3.146LysPro: 3.146 ± 0.354
2.211LysGln: 2.211 ± 0.307
3.996LysArg: 3.996 ± 0.462
2.296LysSer: 2.296 ± 0.325
1.913LysThr: 1.913 ± 0.25
3.103LysVal: 3.103 ± 0.417
1.53LysTrp: 1.53 ± 0.251
1.488LysTyr: 1.488 ± 0.247
0.0LysXaa: 0.0 ± 0.0
Leu
8.757LeuAla: 8.757 ± 0.598
0.85LeuCys: 0.85 ± 0.184
5.781LeuAsp: 5.781 ± 0.43
4.208LeuGlu: 4.208 ± 0.392
2.04LeuPhe: 2.04 ± 0.337
5.229LeuGly: 5.229 ± 0.401
1.445LeuHis: 1.445 ± 0.263
3.486LeuIle: 3.486 ± 0.41
4.846LeuLys: 4.846 ± 0.474
6.206LeuLeu: 6.206 ± 0.543
2.551LeuMet: 2.551 ± 0.357
4.208LeuAsn: 4.208 ± 0.56
5.229LeuPro: 5.229 ± 0.652
2.593LeuGln: 2.593 ± 0.333
4.846LeuArg: 4.846 ± 0.441
6.036LeuSer: 6.036 ± 0.526
5.611LeuThr: 5.611 ± 0.518
6.206LeuVal: 6.206 ± 0.48
2.211LeuTrp: 2.211 ± 0.35
1.955LeuTyr: 1.955 ± 0.262
0.0LeuXaa: 0.0 ± 0.0
Met
2.933MetAla: 2.933 ± 0.411
0.425MetCys: 0.425 ± 0.127
1.105MetAsp: 1.105 ± 0.225
0.893MetGlu: 0.893 ± 0.216
0.893MetPhe: 0.893 ± 0.201
1.913MetGly: 1.913 ± 0.238
0.553MetHis: 0.553 ± 0.15
0.68MetIle: 0.68 ± 0.135
0.935MetLys: 0.935 ± 0.198
1.785MetLeu: 1.785 ± 0.275
0.383MetMet: 0.383 ± 0.1
0.68MetAsn: 0.68 ± 0.148
1.53MetPro: 1.53 ± 0.217
0.765MetGln: 0.765 ± 0.174
1.7MetArg: 1.7 ± 0.277
2.848MetSer: 2.848 ± 0.401
1.828MetThr: 1.828 ± 0.223
1.233MetVal: 1.233 ± 0.276
0.51MetTrp: 0.51 ± 0.139
0.765MetTyr: 0.765 ± 0.173
0.0MetXaa: 0.0 ± 0.0
Asn
3.996AsnAla: 3.996 ± 0.437
0.128AsnCys: 0.128 ± 0.077
2.338AsnAsp: 2.338 ± 0.277
2.296AsnGlu: 2.296 ± 0.327
0.935AsnPhe: 0.935 ± 0.199
4.123AsnGly: 4.123 ± 0.481
0.553AsnHis: 0.553 ± 0.138
0.723AsnIle: 0.723 ± 0.166
1.36AsnLys: 1.36 ± 0.236
3.613AsnLeu: 3.613 ± 0.453
1.105AsnMet: 1.105 ± 0.22
1.53AsnAsn: 1.53 ± 0.288
3.103AsnPro: 3.103 ± 0.385
0.978AsnGln: 0.978 ± 0.268
2.423AsnArg: 2.423 ± 0.336
1.573AsnSer: 1.573 ± 0.243
1.998AsnThr: 1.998 ± 0.279
3.103AsnVal: 3.103 ± 0.333
0.553AsnTrp: 0.553 ± 0.113
1.02AsnTyr: 1.02 ± 0.179
0.0AsnXaa: 0.0 ± 0.0
Pro
4.506ProAla: 4.506 ± 0.429
0.51ProCys: 0.51 ± 0.201
4.719ProAsp: 4.719 ± 0.457
3.188ProGlu: 3.188 ± 0.379
1.148ProPhe: 1.148 ± 0.194
5.186ProGly: 5.186 ± 0.734
0.723ProHis: 0.723 ± 0.17
2.083ProIle: 2.083 ± 0.31
2.721ProLys: 2.721 ± 0.362
3.061ProLeu: 3.061 ± 0.339
0.893ProMet: 0.893 ± 0.187
2.423ProAsn: 2.423 ± 0.244
2.253ProPro: 2.253 ± 0.341
1.785ProGln: 1.785 ± 0.293
2.593ProArg: 2.593 ± 0.351
2.508ProSer: 2.508 ± 0.317
3.146ProThr: 3.146 ± 0.374
4.208ProVal: 4.208 ± 0.419
1.19ProTrp: 1.19 ± 0.197
1.318ProTyr: 1.318 ± 0.213
0.0ProXaa: 0.0 ± 0.0
Gln
5.144GlnAla: 5.144 ± 0.465
0.553GlnCys: 0.553 ± 0.168
1.53GlnAsp: 1.53 ± 0.25
1.233GlnGlu: 1.233 ± 0.245
1.53GlnPhe: 1.53 ± 0.25
2.891GlnGly: 2.891 ± 0.302
0.978GlnHis: 0.978 ± 0.23
1.36GlnIle: 1.36 ± 0.251
1.743GlnLys: 1.743 ± 0.298
2.678GlnLeu: 2.678 ± 0.413
1.063GlnMet: 1.063 ± 0.222
1.403GlnAsn: 1.403 ± 0.255
1.488GlnPro: 1.488 ± 0.211
1.913GlnGln: 1.913 ± 0.438
2.423GlnArg: 2.423 ± 0.388
1.785GlnSer: 1.785 ± 0.281
1.658GlnThr: 1.658 ± 0.274
2.551GlnVal: 2.551 ± 0.351
0.935GlnTrp: 0.935 ± 0.166
1.105GlnTyr: 1.105 ± 0.223
0.0GlnXaa: 0.0 ± 0.0
Arg
5.696ArgAla: 5.696 ± 0.497
0.85ArgCys: 0.85 ± 0.205
4.464ArgAsp: 4.464 ± 0.439
4.379ArgGlu: 4.379 ± 0.475
1.998ArgPhe: 1.998 ± 0.284
4.889ArgGly: 4.889 ± 0.523
1.233ArgHis: 1.233 ± 0.224
2.551ArgIle: 2.551 ± 0.345
3.826ArgLys: 3.826 ± 0.539
4.464ArgLeu: 4.464 ± 0.441
1.658ArgMet: 1.658 ± 0.242
2.296ArgAsn: 2.296 ± 0.27
2.253ArgPro: 2.253 ± 0.385
1.955ArgGln: 1.955 ± 0.324
4.379ArgArg: 4.379 ± 0.633
3.316ArgSer: 3.316 ± 0.348
3.061ArgThr: 3.061 ± 0.399
4.974ArgVal: 4.974 ± 0.469
1.233ArgTrp: 1.233 ± 0.244
1.913ArgTyr: 1.913 ± 0.325
0.0ArgXaa: 0.0 ± 0.0
Ser
5.611SerAla: 5.611 ± 0.57
0.383SerCys: 0.383 ± 0.113
3.571SerAsp: 3.571 ± 0.417
3.401SerGlu: 3.401 ± 0.346
1.743SerPhe: 1.743 ± 0.303
5.951SerGly: 5.951 ± 0.612
1.02SerHis: 1.02 ± 0.194
2.125SerIle: 2.125 ± 0.266
2.891SerLys: 2.891 ± 0.396
4.676SerLeu: 4.676 ± 0.461
1.445SerMet: 1.445 ± 0.285
2.211SerAsn: 2.211 ± 0.319
2.211SerPro: 2.211 ± 0.334
2.211SerGln: 2.211 ± 0.315
3.443SerArg: 3.443 ± 0.379
2.976SerSer: 2.976 ± 0.424
3.103SerThr: 3.103 ± 0.318
4.846SerVal: 4.846 ± 0.492
1.488SerTrp: 1.488 ± 0.286
1.658SerTyr: 1.658 ± 0.334
0.0SerXaa: 0.0 ± 0.0
Thr
6.589ThrAla: 6.589 ± 0.613
0.34ThrCys: 0.34 ± 0.104
3.868ThrAsp: 3.868 ± 0.381
2.551ThrGlu: 2.551 ± 0.378
2.381ThrPhe: 2.381 ± 0.319
5.016ThrGly: 5.016 ± 0.633
0.978ThrHis: 0.978 ± 0.193
2.508ThrIle: 2.508 ± 0.351
2.551ThrLys: 2.551 ± 0.292
5.016ThrLeu: 5.016 ± 0.436
0.978ThrMet: 0.978 ± 0.202
1.913ThrAsn: 1.913 ± 0.257
3.188ThrPro: 3.188 ± 0.378
1.743ThrGln: 1.743 ± 0.243
2.466ThrArg: 2.466 ± 0.36
2.466ThrSer: 2.466 ± 0.321
3.231ThrThr: 3.231 ± 0.411
5.654ThrVal: 5.654 ± 0.481
1.105ThrTrp: 1.105 ± 0.223
1.7ThrTyr: 1.7 ± 0.288
0.0ThrXaa: 0.0 ± 0.0
Val
7.567ValAla: 7.567 ± 0.501
0.808ValCys: 0.808 ± 0.202
5.654ValAsp: 5.654 ± 0.502
3.826ValGlu: 3.826 ± 0.402
1.87ValPhe: 1.87 ± 0.301
6.036ValGly: 6.036 ± 0.46
1.658ValHis: 1.658 ± 0.26
3.911ValIle: 3.911 ± 0.373
3.656ValLys: 3.656 ± 0.371
5.739ValLeu: 5.739 ± 0.46
1.53ValMet: 1.53 ± 0.257
2.423ValAsn: 2.423 ± 0.311
3.911ValPro: 3.911 ± 0.411
3.061ValGln: 3.061 ± 0.342
4.634ValArg: 4.634 ± 0.411
4.549ValSer: 4.549 ± 0.432
4.464ValThr: 4.464 ± 0.427
6.249ValVal: 6.249 ± 0.609
1.403ValTrp: 1.403 ± 0.25
1.87ValTyr: 1.87 ± 0.331
0.0ValXaa: 0.0 ± 0.0
Trp
1.955TrpAla: 1.955 ± 0.236
0.17TrpCys: 0.17 ± 0.088
1.743TrpAsp: 1.743 ± 0.26
0.978TrpGlu: 0.978 ± 0.199
0.808TrpPhe: 0.808 ± 0.205
1.36TrpGly: 1.36 ± 0.183
0.51TrpHis: 0.51 ± 0.144
1.063TrpIle: 1.063 ± 0.193
0.978TrpLys: 0.978 ± 0.208
2.125TrpLeu: 2.125 ± 0.296
0.51TrpMet: 0.51 ± 0.162
0.935TrpAsn: 0.935 ± 0.199
1.105TrpPro: 1.105 ± 0.243
0.85TrpGln: 0.85 ± 0.179
1.615TrpArg: 1.615 ± 0.253
1.403TrpSer: 1.403 ± 0.301
1.36TrpThr: 1.36 ± 0.316
1.403TrpVal: 1.403 ± 0.292
0.595TrpTrp: 0.595 ± 0.213
0.425TrpTyr: 0.425 ± 0.143
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.763TyrAla: 2.763 ± 0.414
0.213TyrCys: 0.213 ± 0.113
2.083TyrAsp: 2.083 ± 0.332
1.998TyrGlu: 1.998 ± 0.305
0.978TyrPhe: 0.978 ± 0.227
3.018TyrGly: 3.018 ± 0.391
0.553TyrHis: 0.553 ± 0.16
0.85TyrIle: 0.85 ± 0.206
1.148TyrLys: 1.148 ± 0.212
2.806TyrLeu: 2.806 ± 0.356
0.765TyrMet: 0.765 ± 0.219
0.893TyrAsn: 0.893 ± 0.173
1.785TyrPro: 1.785 ± 0.292
1.148TyrGln: 1.148 ± 0.22
1.658TyrArg: 1.658 ± 0.267
1.913TyrSer: 1.913 ± 0.324
1.658TyrThr: 1.658 ± 0.275
1.87TyrVal: 1.87 ± 0.247
0.723TyrTrp: 0.723 ± 0.19
0.595TyrTyr: 0.595 ± 0.145
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 142 proteins (23525 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski