Collinsella sp. AK_207A
Average proteome isoelectric point is 5.84
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1796 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A5K1JIK0|A0A5K1JIK0_9ACTN Lipid A export ATP-binding/permease protein MsbA OS=Collinsella sp. AK_207A OX=2650472 GN=msbA_3 PE=4 SV=1
MM1 pKa = 7.75 AGQTEE6 pKa = 4.63 FSGFPPVRR14 pKa = 11.84 VDD16 pKa = 3.35 LTGLVEE22 pKa = 4.6 NPGEE26 pKa = 4.12 SHH28 pKa = 6.34 AVSGRR33 pKa = 11.84 VEE35 pKa = 3.95 ADD37 pKa = 3.13 SYY39 pKa = 11.48 RR40 pKa = 11.84 VGEE43 pKa = 4.05 KK44 pKa = 10.09 EE45 pKa = 3.69 YY46 pKa = 10.88 RR47 pKa = 11.84 LTDD50 pKa = 3.58 GVSYY54 pKa = 11.18 DD55 pKa = 3.9 VVFTNAGDD63 pKa = 3.95 GVLVSGMVRR72 pKa = 11.84 AHH74 pKa = 7.35 AEE76 pKa = 4.43 GEE78 pKa = 4.05 CDD80 pKa = 3.31 RR81 pKa = 11.84 CLEE84 pKa = 4.19 PASFEE89 pKa = 4.29 VAGEE93 pKa = 3.78 IQEE96 pKa = 4.45 YY97 pKa = 10.66 YY98 pKa = 10.95 LFAEE102 pKa = 4.99 PDD104 pKa = 3.62 DD105 pKa = 4.53 PDD107 pKa = 4.9 AYY109 pKa = 10.44 EE110 pKa = 5.57 DD111 pKa = 3.79 DD112 pKa = 4.89 FEE114 pKa = 5.39 VLGQDD119 pKa = 4.38 RR120 pKa = 11.84 VIDD123 pKa = 4.07 LADD126 pKa = 4.44 SIHH129 pKa = 7.06 DD130 pKa = 3.88 AVVMDD135 pKa = 3.84 TPFVVLCQPDD145 pKa = 4.18 CKK147 pKa = 10.63 GLCPTCGANLNLGDD161 pKa = 4.9 CGCSSHH167 pKa = 6.37 QVEE170 pKa = 4.49 EE171 pKa = 4.64 HH172 pKa = 6.5 AASDD176 pKa = 3.97 DD177 pKa = 3.77 NPFAKK182 pKa = 10.43 LRR184 pKa = 11.84 DD185 pKa = 3.78 LKK187 pKa = 11.16 LDD189 pKa = 3.52 NN190 pKa = 4.63
Molecular weight: 20.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.765
IPC2_protein 3.986
IPC_protein 3.986
Toseland 3.77
ProMoST 4.126
Dawson 3.961
Bjellqvist 4.113
Wikipedia 3.872
Rodwell 3.808
Grimsley 3.681
Solomon 3.961
Lehninger 3.91
Nozaki 4.075
DTASelect 4.291
Thurlkill 3.821
EMBOSS 3.884
Sillero 4.101
Patrickios 1.24
IPC_peptide 3.948
IPC2_peptide 4.075
IPC2.peptide.svr19 3.974
Protein with the highest isoelectric point:
>tr|A0A5K1IUD6|A0A5K1IUD6_9ACTN Phosphoesterase OS=Collinsella sp. AK_207A OX=2650472 GN=LGDEJFIL_00104 PE=3 SV=1
MM1 pKa = 7.71 AVRR4 pKa = 11.84 CTVSAVARR12 pKa = 11.84 ACRR15 pKa = 11.84 IEE17 pKa = 3.76 WHH19 pKa = 6.28 SVGGICGRR27 pKa = 11.84 ILGDD31 pKa = 3.43 LRR33 pKa = 11.84 RR34 pKa = 11.84 ATGPARR40 pKa = 11.84 SDD42 pKa = 3.28 GVRR45 pKa = 11.84 GIGIDD50 pKa = 3.21 EE51 pKa = 4.02 TSYY54 pKa = 11.56 KK55 pKa = 10.05 KK56 pKa = 9.99 GHH58 pKa = 6.8 RR59 pKa = 11.84 YY60 pKa = 10.15 LMVVVDD66 pKa = 4.54 HH67 pKa = 7.75 DD68 pKa = 4.5 RR69 pKa = 11.84 GCLIWAAGGGAKK81 pKa = 9.63 RR82 pKa = 11.84 CCAGSSKK89 pKa = 10.52 RR90 pKa = 11.84 NSPASRR96 pKa = 11.84 GFPSRR101 pKa = 11.84 WW102 pKa = 3.14
Molecular weight: 10.93 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.348
IPC2_protein 9.399
IPC_protein 10.043
Toseland 10.423
ProMoST 10.131
Dawson 10.54
Bjellqvist 10.306
Wikipedia 10.745
Rodwell 10.687
Grimsley 10.584
Solomon 10.657
Lehninger 10.628
Nozaki 10.526
DTASelect 10.262
Thurlkill 10.438
EMBOSS 10.833
Sillero 10.496
Patrickios 10.467
IPC_peptide 10.657
IPC2_peptide 9.809
IPC2.peptide.svr19 8.42
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1796
0
1796
609878
29
2151
339.6
36.8
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.068 ± 0.072
1.398 ± 0.026
6.232 ± 0.04
6.405 ± 0.056
3.56 ± 0.037
8.433 ± 0.046
1.912 ± 0.028
4.861 ± 0.044
3.655 ± 0.063
9.311 ± 0.071
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.598 ± 0.025
2.805 ± 0.041
4.402 ± 0.04
2.873 ± 0.032
6.345 ± 0.072
5.735 ± 0.046
5.441 ± 0.052
8.224 ± 0.043
1.011 ± 0.02
2.732 ± 0.032
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here