Gordonia phage GTE5

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Siphoviridae; unclassified Siphoviridae

Average proteome isoelectric point is 6.36

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 92 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2TLT3|Q2TLT3_9CAUD Uncharacterized protein OS=Gordonia phage GTE5 OX=319522 GN=GTE5p025 PE=4 SV=1
MM1 pKa = 6.71VQSTTVIEE9 pKa = 4.7FGTEE13 pKa = 3.36FWIEE17 pKa = 3.9FGTQQVAPLDD27 pKa = 3.77TEE29 pKa = 3.84ITGRR33 pKa = 11.84RR34 pKa = 11.84VEE36 pKa = 4.26DD37 pKa = 4.2GYY39 pKa = 11.44LIVEE43 pKa = 4.59AWCVYY48 pKa = 10.61DD49 pKa = 4.63YY50 pKa = 11.79GPFSDD55 pKa = 4.23GATPAFDD62 pKa = 4.05FQALLLEE69 pKa = 4.54ASLFGEE75 pKa = 4.67LIEE78 pKa = 4.35PTEE81 pKa = 4.44VTCTSSPDD89 pKa = 3.49GQGARR94 pKa = 11.84ITTIKK99 pKa = 10.19EE100 pKa = 3.69

Molecular weight: 11.02 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8EJN0|G8EJN0_9CAUD Uncharacterized protein OS=Gordonia phage GTE5 OX=319522 PE=4 SV=1
MM1 pKa = 6.82ATRR4 pKa = 11.84RR5 pKa = 11.84RR6 pKa = 11.84RR7 pKa = 11.84GGSLNSRR14 pKa = 11.84KK15 pKa = 9.72KK16 pKa = 10.8GKK18 pKa = 10.18AKK20 pKa = 10.65NGAKK24 pKa = 9.07WGHH27 pKa = 5.7GFVPKK32 pKa = 10.29NAAARR37 pKa = 11.84KK38 pKa = 9.03LKK40 pKa = 10.62KK41 pKa = 10.37KK42 pKa = 10.28LDD44 pKa = 3.46RR45 pKa = 11.84QGRR48 pKa = 11.84RR49 pKa = 11.84RR50 pKa = 11.84KK51 pKa = 8.76RR52 pKa = 11.84TAQGYY57 pKa = 6.78PKK59 pKa = 10.41RR60 pKa = 4.1

Molecular weight: 6.87 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

92

0

92

20381

32

1801

221.5

24.3

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

10.397 ± 0.443

0.898 ± 0.147

6.918 ± 0.278

6.246 ± 0.32

3.13 ± 0.141

8.846 ± 0.347

2.002 ± 0.188

5.019 ± 0.168

4.043 ± 0.221

7.321 ± 0.257

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.296 ± 0.098

3.282 ± 0.173

5.515 ± 0.186

3.292 ± 0.151

7.173 ± 0.343

5.628 ± 0.223

6.418 ± 0.202

7.1 ± 0.191

1.918 ± 0.111

2.556 ± 0.179
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski