Amino acid dipepetide frequency for Gordonia phage GTE5

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
12.758AlaAla: 12.758 ± 1.02
1.079AlaCys: 1.079 ± 0.242
7.655AlaAsp: 7.655 ± 0.789
7.409AlaGlu: 7.409 ± 0.706
3.14AlaPhe: 3.14 ± 0.395
8.979AlaGly: 8.979 ± 0.777
2.453AlaHis: 2.453 ± 0.548
5.054AlaIle: 5.054 ± 0.54
4.809AlaLys: 4.809 ± 0.545
7.655AlaLeu: 7.655 ± 0.79
2.846AlaMet: 2.846 ± 0.407
3.386AlaAsn: 3.386 ± 0.423
3.876AlaPro: 3.876 ± 0.507
3.778AlaGln: 3.778 ± 0.609
5.888AlaArg: 5.888 ± 0.692
5.348AlaSer: 5.348 ± 0.673
6.526AlaThr: 6.526 ± 0.71
7.655AlaVal: 7.655 ± 0.681
1.472AlaTrp: 1.472 ± 0.296
2.159AlaTyr: 2.159 ± 0.354
0.0AlaXaa: 0.0 ± 0.0
Cys
0.736CysAla: 0.736 ± 0.219
0.098CysCys: 0.098 ± 0.079
0.736CysAsp: 0.736 ± 0.204
0.687CysGlu: 0.687 ± 0.18
0.098CysPhe: 0.098 ± 0.074
1.129CysGly: 1.129 ± 0.294
0.393CysHis: 0.393 ± 0.183
0.491CysIle: 0.491 ± 0.152
0.098CysLys: 0.098 ± 0.071
0.589CysLeu: 0.589 ± 0.185
0.147CysMet: 0.147 ± 0.065
0.098CysAsn: 0.098 ± 0.067
0.54CysPro: 0.54 ± 0.154
0.442CysGln: 0.442 ± 0.157
0.343CysArg: 0.343 ± 0.136
0.442CysSer: 0.442 ± 0.188
0.736CysThr: 0.736 ± 0.194
0.736CysVal: 0.736 ± 0.212
0.245CysTrp: 0.245 ± 0.1
0.196CysTyr: 0.196 ± 0.114
0.0CysXaa: 0.0 ± 0.0
Asp
6.183AspAla: 6.183 ± 0.525
0.54AspCys: 0.54 ± 0.158
5.986AspAsp: 5.986 ± 0.96
5.152AspGlu: 5.152 ± 0.66
2.257AspPhe: 2.257 ± 0.386
6.526AspGly: 6.526 ± 0.624
0.981AspHis: 0.981 ± 0.21
3.14AspIle: 3.14 ± 0.514
2.11AspLys: 2.11 ± 0.361
5.005AspLeu: 5.005 ± 0.421
1.227AspMet: 1.227 ± 0.263
2.11AspAsn: 2.11 ± 0.347
5.005AspPro: 5.005 ± 0.514
2.306AspGln: 2.306 ± 0.325
4.858AspArg: 4.858 ± 0.585
3.189AspSer: 3.189 ± 0.45
4.269AspThr: 4.269 ± 0.549
4.76AspVal: 4.76 ± 0.5
1.423AspTrp: 1.423 ± 0.208
2.159AspTyr: 2.159 ± 0.373
0.0AspXaa: 0.0 ± 0.0
Glu
6.82GluAla: 6.82 ± 0.719
0.343GluCys: 0.343 ± 0.148
3.582GluAsp: 3.582 ± 0.439
4.122GluGlu: 4.122 ± 0.691
2.748GluPhe: 2.748 ± 0.391
4.269GluGly: 4.269 ± 0.439
1.963GluHis: 1.963 ± 0.333
3.778GluIle: 3.778 ± 0.494
2.601GluLys: 2.601 ± 0.39
5.496GluLeu: 5.496 ± 0.485
1.374GluMet: 1.374 ± 0.252
2.257GluAsn: 2.257 ± 0.355
2.846GluPro: 2.846 ± 0.454
2.012GluGln: 2.012 ± 0.253
4.024GluArg: 4.024 ± 0.511
3.14GluSer: 3.14 ± 0.427
3.974GluThr: 3.974 ± 0.521
4.416GluVal: 4.416 ± 0.626
0.981GluTrp: 0.981 ± 0.175
1.717GluTyr: 1.717 ± 0.302
0.0GluXaa: 0.0 ± 0.0
Phe
2.65PheAla: 2.65 ± 0.362
0.393PheCys: 0.393 ± 0.159
3.14PheAsp: 3.14 ± 0.381
2.061PheGlu: 2.061 ± 0.321
0.883PhePhe: 0.883 ± 0.277
3.042PheGly: 3.042 ± 0.349
0.834PheHis: 0.834 ± 0.2
1.57PheIle: 1.57 ± 0.22
0.932PheLys: 0.932 ± 0.167
2.159PheLeu: 2.159 ± 0.332
0.736PheMet: 0.736 ± 0.207
0.687PheAsn: 0.687 ± 0.183
1.129PhePro: 1.129 ± 0.296
1.276PheGln: 1.276 ± 0.255
2.11PheArg: 2.11 ± 0.308
1.914PheSer: 1.914 ± 0.275
1.865PheThr: 1.865 ± 0.265
2.061PheVal: 2.061 ± 0.36
0.687PheTrp: 0.687 ± 0.17
1.178PheTyr: 1.178 ± 0.233
0.0PheXaa: 0.0 ± 0.0
Gly
8.685GlyAla: 8.685 ± 0.855
0.589GlyCys: 0.589 ± 0.162
5.888GlyAsp: 5.888 ± 0.482
4.563GlyGlu: 4.563 ± 0.512
3.14GlyPhe: 3.14 ± 0.439
8.783GlyGly: 8.783 ± 1.456
1.963GlyHis: 1.963 ± 0.37
4.22GlyIle: 4.22 ± 0.66
3.729GlyLys: 3.729 ± 0.483
6.526GlyLeu: 6.526 ± 0.553
1.668GlyMet: 1.668 ± 0.37
3.042GlyAsn: 3.042 ± 0.5
4.956GlyPro: 4.956 ± 0.577
2.846GlyGln: 2.846 ± 0.45
5.839GlyArg: 5.839 ± 0.552
5.692GlySer: 5.692 ± 0.637
5.937GlyThr: 5.937 ± 0.74
5.888GlyVal: 5.888 ± 0.549
1.816GlyTrp: 1.816 ± 0.348
2.699GlyTyr: 2.699 ± 0.379
0.0GlyXaa: 0.0 ± 0.0
His
1.963HisAla: 1.963 ± 0.355
0.0HisCys: 0.0 ± 0.0
1.178HisAsp: 1.178 ± 0.264
0.687HisGlu: 0.687 ± 0.213
0.785HisPhe: 0.785 ± 0.198
1.57HisGly: 1.57 ± 0.314
0.442HisHis: 0.442 ± 0.145
0.932HisIle: 0.932 ± 0.176
0.343HisLys: 0.343 ± 0.119
1.521HisLeu: 1.521 ± 0.25
0.491HisMet: 0.491 ± 0.141
0.54HisAsn: 0.54 ± 0.144
1.472HisPro: 1.472 ± 0.251
0.442HisGln: 0.442 ± 0.151
2.11HisArg: 2.11 ± 0.382
1.178HisSer: 1.178 ± 0.249
1.423HisThr: 1.423 ± 0.324
1.521HisVal: 1.521 ± 0.271
0.442HisTrp: 0.442 ± 0.141
0.981HisTyr: 0.981 ± 0.238
0.0HisXaa: 0.0 ± 0.0
Ile
5.937IleAla: 5.937 ± 0.491
0.393IleCys: 0.393 ± 0.142
3.729IleAsp: 3.729 ± 0.45
4.22IleGlu: 4.22 ± 0.532
1.276IlePhe: 1.276 ± 0.255
4.809IleGly: 4.809 ± 0.683
1.03IleHis: 1.03 ± 0.24
2.11IleIle: 2.11 ± 0.295
1.963IleLys: 1.963 ± 0.276
3.189IleLeu: 3.189 ± 0.38
0.883IleMet: 0.883 ± 0.177
2.159IleAsn: 2.159 ± 0.376
2.993IlePro: 2.993 ± 0.344
1.423IleGln: 1.423 ± 0.255
2.993IleArg: 2.993 ± 0.365
2.748IleSer: 2.748 ± 0.356
3.582IleThr: 3.582 ± 0.452
2.895IleVal: 2.895 ± 0.409
0.785IleTrp: 0.785 ± 0.183
1.079IleTyr: 1.079 ± 0.264
0.0IleXaa: 0.0 ± 0.0
Lys
3.484LysAla: 3.484 ± 0.485
0.245LysCys: 0.245 ± 0.123
2.748LysAsp: 2.748 ± 0.361
1.423LysGlu: 1.423 ± 0.223
1.423LysPhe: 1.423 ± 0.266
3.484LysGly: 3.484 ± 0.457
0.687LysHis: 0.687 ± 0.189
2.257LysIle: 2.257 ± 0.292
2.65LysLys: 2.65 ± 0.5
3.386LysLeu: 3.386 ± 0.47
0.834LysMet: 0.834 ± 0.233
1.325LysAsn: 1.325 ± 0.216
2.257LysPro: 2.257 ± 0.356
1.521LysGln: 1.521 ± 0.274
2.846LysArg: 2.846 ± 0.33
2.061LysSer: 2.061 ± 0.282
2.601LysThr: 2.601 ± 0.396
3.435LysVal: 3.435 ± 0.4
0.736LysTrp: 0.736 ± 0.184
1.03LysTyr: 1.03 ± 0.212
0.0LysXaa: 0.0 ± 0.0
Leu
7.409LeuAla: 7.409 ± 0.846
1.129LeuCys: 1.129 ± 0.295
5.25LeuAsp: 5.25 ± 0.537
3.68LeuGlu: 3.68 ± 0.416
2.11LeuPhe: 2.11 ± 0.32
5.937LeuGly: 5.937 ± 0.573
1.276LeuHis: 1.276 ± 0.244
3.729LeuIle: 3.729 ± 0.407
2.797LeuLys: 2.797 ± 0.378
5.348LeuLeu: 5.348 ± 0.432
1.668LeuMet: 1.668 ± 0.255
2.355LeuAsn: 2.355 ± 0.352
4.661LeuPro: 4.661 ± 0.483
2.208LeuGln: 2.208 ± 0.342
5.447LeuArg: 5.447 ± 0.434
4.907LeuSer: 4.907 ± 0.355
4.956LeuThr: 4.956 ± 0.499
4.956LeuVal: 4.956 ± 0.514
1.276LeuTrp: 1.276 ± 0.278
2.11LeuTyr: 2.11 ± 0.321
0.0LeuXaa: 0.0 ± 0.0
Met
3.042MetAla: 3.042 ± 0.47
0.147MetCys: 0.147 ± 0.08
1.178MetAsp: 1.178 ± 0.225
1.521MetGlu: 1.521 ± 0.241
0.932MetPhe: 0.932 ± 0.25
1.178MetGly: 1.178 ± 0.248
0.245MetHis: 0.245 ± 0.12
1.03MetIle: 1.03 ± 0.237
1.079MetLys: 1.079 ± 0.222
1.668MetLeu: 1.668 ± 0.278
0.442MetMet: 0.442 ± 0.135
0.589MetAsn: 0.589 ± 0.153
1.865MetPro: 1.865 ± 0.226
0.54MetGln: 0.54 ± 0.146
1.472MetArg: 1.472 ± 0.265
2.306MetSer: 2.306 ± 0.425
1.57MetThr: 1.57 ± 0.265
0.981MetVal: 0.981 ± 0.19
0.343MetTrp: 0.343 ± 0.127
0.834MetTyr: 0.834 ± 0.194
0.0MetXaa: 0.0 ± 0.0
Asn
3.189AsnAla: 3.189 ± 0.412
0.196AsnCys: 0.196 ± 0.087
2.404AsnAsp: 2.404 ± 0.386
1.963AsnGlu: 1.963 ± 0.289
1.227AsnPhe: 1.227 ± 0.232
3.533AsnGly: 3.533 ± 0.403
0.589AsnHis: 0.589 ± 0.213
1.717AsnIle: 1.717 ± 0.301
1.178AsnLys: 1.178 ± 0.218
1.766AsnLeu: 1.766 ± 0.271
0.736AsnMet: 0.736 ± 0.191
1.079AsnAsn: 1.079 ± 0.265
2.404AsnPro: 2.404 ± 0.368
1.129AsnGln: 1.129 ± 0.221
1.914AsnArg: 1.914 ± 0.297
1.57AsnSer: 1.57 ± 0.255
1.717AsnThr: 1.717 ± 0.292
2.552AsnVal: 2.552 ± 0.324
0.883AsnTrp: 0.883 ± 0.184
1.079AsnTyr: 1.079 ± 0.207
0.0AsnXaa: 0.0 ± 0.0
Pro
5.888ProAla: 5.888 ± 0.615
0.343ProCys: 0.343 ± 0.115
4.122ProAsp: 4.122 ± 0.409
3.974ProGlu: 3.974 ± 0.604
1.423ProPhe: 1.423 ± 0.224
5.201ProGly: 5.201 ± 0.624
0.981ProHis: 0.981 ± 0.204
2.993ProIle: 2.993 ± 0.337
2.404ProLys: 2.404 ± 0.357
3.876ProLeu: 3.876 ± 0.519
1.374ProMet: 1.374 ± 0.212
1.914ProAsn: 1.914 ± 0.252
2.993ProPro: 2.993 ± 0.472
1.472ProGln: 1.472 ± 0.266
4.073ProArg: 4.073 ± 0.556
2.846ProSer: 2.846 ± 0.338
2.993ProThr: 2.993 ± 0.447
4.465ProVal: 4.465 ± 0.598
0.834ProTrp: 0.834 ± 0.21
0.981ProTyr: 0.981 ± 0.228
0.0ProXaa: 0.0 ± 0.0
Gln
3.68GlnAla: 3.68 ± 0.661
0.442GlnCys: 0.442 ± 0.135
1.57GlnAsp: 1.57 ± 0.253
1.963GlnGlu: 1.963 ± 0.321
0.932GlnPhe: 0.932 ± 0.216
2.208GlnGly: 2.208 ± 0.33
0.491GlnHis: 0.491 ± 0.132
1.816GlnIle: 1.816 ± 0.276
1.129GlnLys: 1.129 ± 0.191
2.601GlnLeu: 2.601 ± 0.315
1.276GlnMet: 1.276 ± 0.279
1.325GlnAsn: 1.325 ± 0.301
1.668GlnPro: 1.668 ± 0.321
1.619GlnGln: 1.619 ± 0.32
2.552GlnArg: 2.552 ± 0.359
1.914GlnSer: 1.914 ± 0.366
1.668GlnThr: 1.668 ± 0.245
2.552GlnVal: 2.552 ± 0.371
0.785GlnTrp: 0.785 ± 0.159
0.736GlnTyr: 0.736 ± 0.174
0.0GlnXaa: 0.0 ± 0.0
Arg
6.722ArgAla: 6.722 ± 0.663
0.834ArgCys: 0.834 ± 0.223
4.612ArgAsp: 4.612 ± 0.525
4.809ArgGlu: 4.809 ± 0.631
2.601ArgPhe: 2.601 ± 0.456
6.428ArgGly: 6.428 ± 0.642
1.717ArgHis: 1.717 ± 0.313
3.238ArgIle: 3.238 ± 0.415
3.337ArgLys: 3.337 ± 0.43
4.858ArgLeu: 4.858 ± 0.455
2.061ArgMet: 2.061 ± 0.356
2.159ArgAsn: 2.159 ± 0.305
3.288ArgPro: 3.288 ± 0.335
2.846ArgGln: 2.846 ± 0.3
7.36ArgArg: 7.36 ± 0.796
2.993ArgSer: 2.993 ± 0.506
4.024ArgThr: 4.024 ± 0.379
5.005ArgVal: 5.005 ± 0.499
1.423ArgTrp: 1.423 ± 0.245
1.423ArgTyr: 1.423 ± 0.266
0.0ArgXaa: 0.0 ± 0.0
Ser
7.213SerAla: 7.213 ± 0.65
0.589SerCys: 0.589 ± 0.182
3.533SerAsp: 3.533 ± 0.457
3.337SerGlu: 3.337 ± 0.398
1.227SerPhe: 1.227 ± 0.238
5.692SerGly: 5.692 ± 0.561
0.687SerHis: 0.687 ± 0.172
2.748SerIle: 2.748 ± 0.342
2.012SerLys: 2.012 ± 0.283
3.484SerLeu: 3.484 ± 0.298
1.766SerMet: 1.766 ± 0.325
1.816SerAsn: 1.816 ± 0.396
2.748SerPro: 2.748 ± 0.406
1.766SerGln: 1.766 ± 0.354
4.073SerArg: 4.073 ± 0.596
3.337SerSer: 3.337 ± 0.502
3.827SerThr: 3.827 ± 0.473
3.631SerVal: 3.631 ± 0.383
1.472SerTrp: 1.472 ± 0.282
1.325SerTyr: 1.325 ± 0.235
0.0SerXaa: 0.0 ± 0.0
Thr
6.869ThrAla: 6.869 ± 0.68
0.589ThrCys: 0.589 ± 0.184
3.631ThrAsp: 3.631 ± 0.42
3.68ThrGlu: 3.68 ± 0.443
1.766ThrPhe: 1.766 ± 0.308
6.281ThrGly: 6.281 ± 0.524
0.932ThrHis: 0.932 ± 0.246
3.582ThrIle: 3.582 ± 0.423
3.042ThrLys: 3.042 ± 0.317
4.76ThrLeu: 4.76 ± 0.426
1.129ThrMet: 1.129 ± 0.239
2.159ThrAsn: 2.159 ± 0.338
3.925ThrPro: 3.925 ± 0.436
1.914ThrGln: 1.914 ± 0.248
4.171ThrArg: 4.171 ± 0.398
2.944ThrSer: 2.944 ± 0.358
4.711ThrThr: 4.711 ± 0.593
4.956ThrVal: 4.956 ± 0.446
1.227ThrTrp: 1.227 ± 0.205
1.914ThrTyr: 1.914 ± 0.332
0.0ThrXaa: 0.0 ± 0.0
Val
6.869ValAla: 6.869 ± 0.684
0.491ValCys: 0.491 ± 0.223
4.858ValAsp: 4.858 ± 0.393
4.465ValGlu: 4.465 ± 0.507
1.963ValPhe: 1.963 ± 0.315
6.379ValGly: 6.379 ± 0.809
1.521ValHis: 1.521 ± 0.274
4.269ValIle: 4.269 ± 0.414
2.601ValLys: 2.601 ± 0.385
4.907ValLeu: 4.907 ± 0.504
1.57ValMet: 1.57 ± 0.313
2.404ValAsn: 2.404 ± 0.302
3.827ValPro: 3.827 ± 0.442
2.404ValGln: 2.404 ± 0.327
5.888ValArg: 5.888 ± 0.527
4.269ValSer: 4.269 ± 0.329
4.858ValThr: 4.858 ± 0.474
5.152ValVal: 5.152 ± 0.514
1.129ValTrp: 1.129 ± 0.219
1.178ValTyr: 1.178 ± 0.256
0.0ValXaa: 0.0 ± 0.0
Trp
1.472TrpAla: 1.472 ± 0.316
0.245TrpCys: 0.245 ± 0.118
1.668TrpAsp: 1.668 ± 0.247
1.178TrpGlu: 1.178 ± 0.253
0.54TrpPhe: 0.54 ± 0.166
1.079TrpGly: 1.079 ± 0.237
0.442TrpHis: 0.442 ± 0.154
0.981TrpIle: 0.981 ± 0.206
0.785TrpLys: 0.785 ± 0.178
1.766TrpLeu: 1.766 ± 0.371
0.343TrpMet: 0.343 ± 0.143
0.638TrpAsn: 0.638 ± 0.182
0.54TrpPro: 0.54 ± 0.169
0.687TrpGln: 0.687 ± 0.199
1.079TrpArg: 1.079 ± 0.265
1.865TrpSer: 1.865 ± 0.333
1.325TrpThr: 1.325 ± 0.251
1.717TrpVal: 1.717 ± 0.239
0.589TrpTrp: 0.589 ± 0.17
0.245TrpTyr: 0.245 ± 0.096
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.404TyrAla: 2.404 ± 0.362
0.294TyrCys: 0.294 ± 0.127
1.717TyrAsp: 1.717 ± 0.315
1.57TyrGlu: 1.57 ± 0.268
0.834TyrPhe: 0.834 ± 0.192
1.963TyrGly: 1.963 ± 0.298
0.393TyrHis: 0.393 ± 0.129
0.54TyrIle: 0.54 ± 0.175
0.834TyrLys: 0.834 ± 0.196
2.65TyrLeu: 2.65 ± 0.413
0.343TyrMet: 0.343 ± 0.119
0.785TyrAsn: 0.785 ± 0.187
2.159TyrPro: 2.159 ± 0.382
0.294TyrGln: 0.294 ± 0.131
2.699TyrArg: 2.699 ± 0.321
1.619TyrSer: 1.619 ± 0.239
1.619TyrThr: 1.619 ± 0.248
1.668TyrVal: 1.668 ± 0.243
0.638TyrTrp: 0.638 ± 0.23
0.54TyrTyr: 0.54 ± 0.157
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 92 proteins (20381 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski