Synechococcus phage S-CAM8

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Myoviridae; Acionnavirus; unclassified Acionnavirus

Average proteome isoelectric point is 5.63

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 204 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|G8EXL5|G8EXL5_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM8 OX=754038 GN=SXCG_00168 PE=4 SV=1
MM1 pKa = 7.74AYY3 pKa = 9.73YY4 pKa = 10.45YY5 pKa = 9.97PEE7 pKa = 5.61GYY9 pKa = 10.04FGPICDD15 pKa = 4.56TPLSDD20 pKa = 5.45DD21 pKa = 4.43QISSQQRR28 pKa = 11.84DD29 pKa = 3.5TPAFDD34 pKa = 3.28EE35 pKa = 4.86GEE37 pKa = 4.12EE38 pKa = 4.09EE39 pKa = 4.72RR40 pKa = 11.84VQNYY44 pKa = 8.04PAEE47 pKa = 4.58GNSDD51 pKa = 3.59IPWWFAYY58 pKa = 9.83KK59 pKa = 10.51DD60 pKa = 3.41ITGDD64 pKa = 3.59LPSVLISTRR73 pKa = 11.84CKK75 pKa = 9.97QRR77 pKa = 11.84EE78 pKa = 4.07DD79 pKa = 3.65GSFFDD84 pKa = 5.59CEE86 pKa = 4.03YY87 pKa = 10.48EE88 pKa = 4.17YY89 pKa = 11.24ASPVDD94 pKa = 4.07FPEE97 pKa = 4.63INVPQEE103 pKa = 3.88DD104 pKa = 4.01FGLGEE109 pKa = 4.0QFFVPVITPEE119 pKa = 3.9SCSPFDD125 pKa = 3.95ADD127 pKa = 5.3INILPISFITPSGGLVTKK145 pKa = 9.92YY146 pKa = 10.22KK147 pKa = 10.27KK148 pKa = 10.16EE149 pKa = 3.8KK150 pKa = 10.2SSPVTYY156 pKa = 10.51AVTSTRR162 pKa = 11.84GFTEE166 pKa = 4.01VQSDD170 pKa = 4.13LDD172 pKa = 3.57VSFNDD177 pKa = 3.77EE178 pKa = 3.92GTQVIVTGTGSAIVTFEE195 pKa = 4.83LRR197 pKa = 11.84WDD199 pKa = 4.41DD200 pKa = 4.9DD201 pKa = 4.15PGTSGLAVGTLTIEE215 pKa = 4.48GQSVSQSGEE224 pKa = 3.93EE225 pKa = 4.4GSTAMSVQLSSGTYY239 pKa = 8.82PISLSGEE246 pKa = 4.1SSGSGSRR253 pKa = 11.84TRR255 pKa = 11.84NNNKK259 pKa = 9.1TIEE262 pKa = 4.13YY263 pKa = 10.28DD264 pKa = 3.9DD265 pKa = 6.44DD266 pKa = 4.29IDD268 pKa = 4.79EE269 pKa = 6.06DD270 pKa = 4.42GFDD273 pKa = 3.85VNATLSIQSVDD284 pKa = 4.03NIDD287 pKa = 4.68GIQIDD292 pKa = 4.64SFWSDD297 pKa = 2.73EE298 pKa = 4.03GNAYY302 pKa = 9.94AVWTNPEE309 pKa = 3.97TCTLPRR315 pKa = 11.84EE316 pKa = 4.4EE317 pKa = 4.56QDD319 pKa = 3.15VTYY322 pKa = 10.38KK323 pKa = 10.56IPIPTAGTYY332 pKa = 10.84GFTFASDD339 pKa = 3.4DD340 pKa = 3.76GGTVIINEE348 pKa = 4.03NDD350 pKa = 3.71TIFNNLPGGIFKK362 pKa = 10.65SGSLSTPYY370 pKa = 10.55SATRR374 pKa = 11.84NLDD377 pKa = 3.31AGILEE382 pKa = 4.21MTVRR386 pKa = 11.84CTNSDD391 pKa = 3.06AGFTTDD397 pKa = 4.94GEE399 pKa = 4.48PSGLAYY405 pKa = 10.25AWWRR409 pKa = 11.84NPGGWYY415 pKa = 9.82IKK417 pKa = 9.19ICKK420 pKa = 10.07GGTCAGEE427 pKa = 4.32TIVPWVRR434 pKa = 11.84SGPHH438 pKa = 4.84QLWSEE443 pKa = 3.78FMNNYY448 pKa = 9.73AVYY451 pKa = 9.59TSNTEE456 pKa = 3.9PSLGTHH462 pKa = 4.94TTSYY466 pKa = 10.16TIYY469 pKa = 10.05IDD471 pKa = 4.67EE472 pKa = 4.56EE473 pKa = 4.38DD474 pKa = 4.62DD475 pKa = 4.19YY476 pKa = 11.47LLEE479 pKa = 4.2MQADD483 pKa = 4.05NNGTISWDD491 pKa = 3.24GTQVLTSSSYY501 pKa = 7.71TTSSTYY507 pKa = 10.0TIPNVSVGPHH517 pKa = 5.08TLSVSVTNVANGGTDD532 pKa = 2.92IDD534 pKa = 3.69TWGNNPGGIAWTLTQPSDD552 pKa = 3.34TVTTTEE558 pKa = 4.2TVTTEE563 pKa = 4.15TPNEE567 pKa = 3.71IQATFNSDD575 pKa = 2.35GDD577 pKa = 3.9IVVTGFGTGRR587 pKa = 11.84IQLIFEE593 pKa = 4.47WDD595 pKa = 3.8DD596 pKa = 3.42DD597 pKa = 4.23PNRR600 pKa = 11.84YY601 pKa = 7.52GTALGSIEE609 pKa = 4.05IAGKK613 pKa = 8.31TFVQTTGKK621 pKa = 10.07EE622 pKa = 4.0RR623 pKa = 11.84GNDD626 pKa = 3.13SYY628 pKa = 11.75TFTARR633 pKa = 11.84AGRR636 pKa = 11.84TYY638 pKa = 10.51TAVIEE643 pKa = 4.5DD644 pKa = 4.02NPSGFTRR651 pKa = 11.84KK652 pKa = 9.64RR653 pKa = 11.84SNTRR657 pKa = 11.84LCFFDD662 pKa = 6.14SDD664 pKa = 5.82DD665 pKa = 4.59DD666 pKa = 4.18DD667 pKa = 6.04CNAKK671 pKa = 10.28LDD673 pKa = 4.05IASVTYY679 pKa = 10.44SGTVSSSSTDD689 pKa = 3.21TNTTTIEE696 pKa = 3.68EE697 pKa = 4.41SIIARR702 pKa = 11.84STDD705 pKa = 3.6LNIGSNIDD713 pKa = 3.44NNNLIWHH720 pKa = 6.11TRR722 pKa = 11.84LATGYY727 pKa = 9.91EE728 pKa = 4.27YY729 pKa = 10.28STTQQ733 pKa = 2.97

Molecular weight: 79.76 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|G8EXQ7|G8EXQ7_9CAUD Uncharacterized protein OS=Synechococcus phage S-CAM8 OX=754038 GN=SXCG_00008 PE=4 SV=1
MM1 pKa = 7.52AVRR4 pKa = 11.84SKK6 pKa = 11.0SLSGGNLIQSKK17 pKa = 9.43PKK19 pKa = 10.2KK20 pKa = 9.5SRR22 pKa = 11.84QGSGQHH28 pKa = 5.15TKK30 pKa = 10.34YY31 pKa = 10.86ASSSRR36 pKa = 11.84NNAKK40 pKa = 9.85KK41 pKa = 10.23RR42 pKa = 11.84YY43 pKa = 8.5RR44 pKa = 11.84GQGRR48 pKa = 3.34

Molecular weight: 5.28 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

204

0

204

54297

48

6167

266.2

29.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.89 ± 0.215

0.88 ± 0.099

6.68 ± 0.135

6.166 ± 0.242

4.221 ± 0.114

7.918 ± 0.31

1.37 ± 0.116

6.435 ± 0.19

5.709 ± 0.417

7.22 ± 0.132

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.089 ± 0.227

5.785 ± 0.176

3.825 ± 0.137

3.761 ± 0.105

3.939 ± 0.146

7.43 ± 0.292

7.638 ± 0.411

6.632 ± 0.219

1.147 ± 0.081

4.265 ± 0.138
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski