Amino acid dipepetide frequency for Synechococcus phage S-CAM8

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
6.372AlaAla: 6.372 ± 0.604
0.589AlaCys: 0.589 ± 0.145
4.42AlaAsp: 4.42 ± 0.268
4.015AlaGlu: 4.015 ± 0.279
3.113AlaPhe: 3.113 ± 0.266
6.593AlaGly: 6.593 ± 0.589
0.829AlaHis: 0.829 ± 0.137
4.347AlaIle: 4.347 ± 0.291
3.628AlaLys: 3.628 ± 0.346
4.936AlaLeu: 4.936 ± 0.373
1.381AlaMet: 1.381 ± 0.232
3.96AlaAsn: 3.96 ± 0.31
2.431AlaPro: 2.431 ± 0.188
2.265AlaGln: 2.265 ± 0.199
2.486AlaArg: 2.486 ± 0.273
4.899AlaSer: 4.899 ± 0.355
5.654AlaThr: 5.654 ± 0.543
4.033AlaVal: 4.033 ± 0.305
0.681AlaTrp: 0.681 ± 0.12
2.265AlaTyr: 2.265 ± 0.185
0.0AlaXaa: 0.0 ± 0.0
Cys
0.645CysAla: 0.645 ± 0.115
0.074CysCys: 0.074 ± 0.037
0.645CysAsp: 0.645 ± 0.142
0.516CysGlu: 0.516 ± 0.115
0.534CysPhe: 0.534 ± 0.13
0.516CysGly: 0.516 ± 0.101
0.239CysHis: 0.239 ± 0.069
0.571CysIle: 0.571 ± 0.126
0.589CysLys: 0.589 ± 0.116
0.516CysLeu: 0.516 ± 0.123
0.239CysMet: 0.239 ± 0.07
0.46CysAsn: 0.46 ± 0.101
0.35CysPro: 0.35 ± 0.082
0.35CysGln: 0.35 ± 0.094
0.405CysArg: 0.405 ± 0.095
0.608CysSer: 0.608 ± 0.142
0.516CysThr: 0.516 ± 0.105
0.534CysVal: 0.534 ± 0.108
0.129CysTrp: 0.129 ± 0.045
0.368CysTyr: 0.368 ± 0.082
0.0CysXaa: 0.0 ± 0.0
Asp
6.004AspAla: 6.004 ± 0.433
0.681AspCys: 0.681 ± 0.132
4.457AspAsp: 4.457 ± 0.408
4.052AspGlu: 4.052 ± 0.35
2.855AspPhe: 2.855 ± 0.227
5.857AspGly: 5.857 ± 0.437
0.976AspHis: 0.976 ± 0.166
4.586AspIle: 4.586 ± 0.298
3.407AspLys: 3.407 ± 0.357
4.604AspLeu: 4.604 ± 0.295
1.565AspMet: 1.565 ± 0.217
3.481AspAsn: 3.481 ± 0.239
3.205AspPro: 3.205 ± 0.292
2.026AspGln: 2.026 ± 0.166
2.468AspArg: 2.468 ± 0.27
3.923AspSer: 3.923 ± 0.302
4.641AspThr: 4.641 ± 0.36
3.978AspVal: 3.978 ± 0.266
1.068AspTrp: 1.068 ± 0.145
2.965AspTyr: 2.965 ± 0.217
0.0AspXaa: 0.0 ± 0.0
Glu
3.463GluAla: 3.463 ± 0.322
0.663GluCys: 0.663 ± 0.096
4.347GluAsp: 4.347 ± 0.315
4.604GluGlu: 4.604 ± 0.478
3.039GluPhe: 3.039 ± 0.21
3.978GluGly: 3.978 ± 0.316
0.921GluHis: 0.921 ± 0.182
4.475GluIle: 4.475 ± 0.304
3.647GluLys: 3.647 ± 0.44
5.194GluLeu: 5.194 ± 0.343
1.529GluMet: 1.529 ± 0.224
3.352GluAsn: 3.352 ± 0.26
1.787GluPro: 1.787 ± 0.173
2.505GluGln: 2.505 ± 0.248
2.836GluArg: 2.836 ± 0.293
3.26GluSer: 3.26 ± 0.296
3.684GluThr: 3.684 ± 0.266
4.549GluVal: 4.549 ± 0.277
0.866GluTrp: 0.866 ± 0.137
2.965GluTyr: 2.965 ± 0.193
0.0GluXaa: 0.0 ± 0.0
Phe
2.763PheAla: 2.763 ± 0.261
0.553PheCys: 0.553 ± 0.112
3.665PheAsp: 3.665 ± 0.266
2.597PheGlu: 2.597 ± 0.215
1.823PhePhe: 1.823 ± 0.201
3.131PheGly: 3.131 ± 0.253
0.516PheHis: 0.516 ± 0.1
2.671PheIle: 2.671 ± 0.242
2.081PheLys: 2.081 ± 0.249
3.039PheLeu: 3.039 ± 0.269
0.847PheMet: 0.847 ± 0.115
2.855PheAsn: 2.855 ± 0.256
1.492PhePro: 1.492 ± 0.191
1.676PheGln: 1.676 ± 0.175
1.4PheArg: 1.4 ± 0.171
3.426PheSer: 3.426 ± 0.234
3.149PheThr: 3.149 ± 0.281
2.597PheVal: 2.597 ± 0.194
0.35PheTrp: 0.35 ± 0.081
1.584PheTyr: 1.584 ± 0.18
0.0PheXaa: 0.0 ± 0.0
Gly
5.949GlyAla: 5.949 ± 0.501
0.571GlyCys: 0.571 ± 0.104
5.765GlyAsp: 5.765 ± 0.479
4.715GlyGlu: 4.715 ± 0.266
2.984GlyPhe: 2.984 ± 0.288
8.122GlyGly: 8.122 ± 1.112
0.958GlyHis: 0.958 ± 0.157
4.641GlyIle: 4.641 ± 0.34
3.628GlyLys: 3.628 ± 0.384
4.383GlyLeu: 4.383 ± 0.31
1.547GlyMet: 1.547 ± 0.279
4.457GlyAsn: 4.457 ± 0.41
2.008GlyPro: 2.008 ± 0.238
2.947GlyGln: 2.947 ± 0.253
2.965GlyArg: 2.965 ± 0.269
6.907GlySer: 6.907 ± 0.826
7.164GlyThr: 7.164 ± 0.731
4.789GlyVal: 4.789 ± 0.32
1.031GlyTrp: 1.031 ± 0.143
3.647GlyTyr: 3.647 ± 0.3
0.0GlyXaa: 0.0 ± 0.0
His
0.571HisAla: 0.571 ± 0.115
0.184HisCys: 0.184 ± 0.057
0.829HisAsp: 0.829 ± 0.128
0.737HisGlu: 0.737 ± 0.15
0.755HisPhe: 0.755 ± 0.133
1.123HisGly: 1.123 ± 0.17
0.239HisHis: 0.239 ± 0.077
0.884HisIle: 0.884 ± 0.141
0.755HisLys: 0.755 ± 0.149
0.939HisLeu: 0.939 ± 0.167
0.332HisMet: 0.332 ± 0.08
0.774HisAsn: 0.774 ± 0.11
0.829HisPro: 0.829 ± 0.161
0.387HisGln: 0.387 ± 0.093
0.571HisArg: 0.571 ± 0.099
0.884HisSer: 0.884 ± 0.137
0.976HisThr: 0.976 ± 0.142
0.939HisVal: 0.939 ± 0.14
0.203HisTrp: 0.203 ± 0.065
0.792HisTyr: 0.792 ± 0.152
0.0HisXaa: 0.0 ± 0.0
Ile
3.997IleAla: 3.997 ± 0.337
0.718IleCys: 0.718 ± 0.12
4.77IleAsp: 4.77 ± 0.396
4.347IleGlu: 4.347 ± 0.281
2.56IlePhe: 2.56 ± 0.253
4.549IleGly: 4.549 ± 0.424
0.755IleHis: 0.755 ± 0.135
3.923IleIle: 3.923 ± 0.324
3.941IleLys: 3.941 ± 0.31
4.604IleLeu: 4.604 ± 0.331
0.902IleMet: 0.902 ± 0.175
4.144IleAsn: 4.144 ± 0.288
2.707IlePro: 2.707 ± 0.245
2.431IleGln: 2.431 ± 0.246
2.118IleArg: 2.118 ± 0.196
4.936IleSer: 4.936 ± 0.367
5.581IleThr: 5.581 ± 0.697
4.42IleVal: 4.42 ± 0.329
0.608IleTrp: 0.608 ± 0.106
2.339IleTyr: 2.339 ± 0.207
0.0IleXaa: 0.0 ± 0.0
Lys
3.094LysAla: 3.094 ± 0.35
0.626LysCys: 0.626 ± 0.141
3.37LysAsp: 3.37 ± 0.333
3.905LysGlu: 3.905 ± 0.481
2.431LysPhe: 2.431 ± 0.227
3.205LysGly: 3.205 ± 0.3
0.958LysHis: 0.958 ± 0.174
4.107LysIle: 4.107 ± 0.286
4.66LysLys: 4.66 ± 0.615
5.157LysLeu: 5.157 ± 0.331
1.584LysMet: 1.584 ± 0.278
2.836LysAsn: 2.836 ± 0.35
1.897LysPro: 1.897 ± 0.237
2.229LysGln: 2.229 ± 0.287
2.339LysArg: 2.339 ± 0.305
3.684LysSer: 3.684 ± 0.313
3.757LysThr: 3.757 ± 0.281
3.868LysVal: 3.868 ± 0.344
0.792LysTrp: 0.792 ± 0.151
2.597LysTyr: 2.597 ± 0.305
0.0LysXaa: 0.0 ± 0.0
Leu
4.254LeuAla: 4.254 ± 0.243
0.81LeuCys: 0.81 ± 0.175
5.709LeuAsp: 5.709 ± 0.335
4.383LeuGlu: 4.383 ± 0.304
2.744LeuPhe: 2.744 ± 0.236
4.733LeuGly: 4.733 ± 0.399
1.142LeuHis: 1.142 ± 0.173
4.347LeuIle: 4.347 ± 0.269
4.383LeuLys: 4.383 ± 0.377
5.12LeuLeu: 5.12 ± 0.368
1.529LeuMet: 1.529 ± 0.259
4.531LeuAsn: 4.531 ± 0.318
2.892LeuPro: 2.892 ± 0.251
3.334LeuGln: 3.334 ± 0.261
3.315LeuArg: 3.315 ± 0.216
5.525LeuSer: 5.525 ± 0.316
5.415LeuThr: 5.415 ± 0.413
4.07LeuVal: 4.07 ± 0.249
0.626LeuTrp: 0.626 ± 0.11
3.334LeuTyr: 3.334 ± 0.292
0.0LeuXaa: 0.0 ± 0.0
Met
1.381MetAla: 1.381 ± 0.214
0.184MetCys: 0.184 ± 0.066
1.05MetAsp: 1.05 ± 0.181
1.197MetGlu: 1.197 ± 0.223
0.829MetPhe: 0.829 ± 0.151
1.344MetGly: 1.344 ± 0.254
0.497MetHis: 0.497 ± 0.109
1.087MetIle: 1.087 ± 0.178
1.823MetLys: 1.823 ± 0.326
1.547MetLeu: 1.547 ± 0.22
0.645MetMet: 0.645 ± 0.134
1.234MetAsn: 1.234 ± 0.146
0.847MetPro: 0.847 ± 0.136
0.958MetGln: 0.958 ± 0.19
1.013MetArg: 1.013 ± 0.204
1.823MetSer: 1.823 ± 0.26
1.676MetThr: 1.676 ± 0.246
0.884MetVal: 0.884 ± 0.13
0.239MetTrp: 0.239 ± 0.071
0.626MetTyr: 0.626 ± 0.108
0.0MetXaa: 0.0 ± 0.0
Asn
4.144AsnAla: 4.144 ± 0.282
0.387AsnCys: 0.387 ± 0.078
3.389AsnAsp: 3.389 ± 0.249
3.131AsnGlu: 3.131 ± 0.193
2.45AsnPhe: 2.45 ± 0.229
4.494AsnGly: 4.494 ± 0.423
0.645AsnHis: 0.645 ± 0.095
3.905AsnIle: 3.905 ± 0.361
3.131AsnLys: 3.131 ± 0.266
4.347AsnLeu: 4.347 ± 0.337
0.958AsnMet: 0.958 ± 0.148
3.37AsnAsn: 3.37 ± 0.377
2.855AsnPro: 2.855 ± 0.226
2.044AsnGln: 2.044 ± 0.208
2.118AsnArg: 2.118 ± 0.182
4.291AsnSer: 4.291 ± 0.428
4.475AsnThr: 4.475 ± 0.392
4.439AsnVal: 4.439 ± 0.329
0.681AsnTrp: 0.681 ± 0.123
2.597AsnTyr: 2.597 ± 0.211
0.0AsnXaa: 0.0 ± 0.0
Pro
2.468ProAla: 2.468 ± 0.211
0.313ProCys: 0.313 ± 0.1
2.21ProAsp: 2.21 ± 0.216
2.947ProGlu: 2.947 ± 0.241
1.658ProPhe: 1.658 ± 0.168
3.334ProGly: 3.334 ± 0.366
0.608ProHis: 0.608 ± 0.112
2.394ProIle: 2.394 ± 0.27
2.155ProLys: 2.155 ± 0.256
2.118ProLeu: 2.118 ± 0.215
0.755ProMet: 0.755 ± 0.165
2.026ProAsn: 2.026 ± 0.2
1.547ProPro: 1.547 ± 0.248
1.437ProGln: 1.437 ± 0.153
1.584ProArg: 1.584 ± 0.141
3.315ProSer: 3.315 ± 0.239
2.781ProThr: 2.781 ± 0.202
2.45ProVal: 2.45 ± 0.272
0.589ProTrp: 0.589 ± 0.119
1.565ProTyr: 1.565 ± 0.178
0.0ProXaa: 0.0 ± 0.0
Gln
2.063GlnAla: 2.063 ± 0.2
0.313GlnCys: 0.313 ± 0.08
2.192GlnAsp: 2.192 ± 0.186
2.523GlnGlu: 2.523 ± 0.257
1.547GlnPhe: 1.547 ± 0.157
2.763GlnGly: 2.763 ± 0.267
0.516GlnHis: 0.516 ± 0.104
2.726GlnIle: 2.726 ± 0.266
2.357GlnLys: 2.357 ± 0.279
3.205GlnLeu: 3.205 ± 0.275
0.958GlnMet: 0.958 ± 0.184
1.86GlnAsn: 1.86 ± 0.188
1.271GlnPro: 1.271 ± 0.154
1.437GlnGln: 1.437 ± 0.172
1.621GlnArg: 1.621 ± 0.166
2.652GlnSer: 2.652 ± 0.202
2.357GlnThr: 2.357 ± 0.313
2.689GlnVal: 2.689 ± 0.216
0.479GlnTrp: 0.479 ± 0.11
2.081GlnTyr: 2.081 ± 0.209
0.0GlnXaa: 0.0 ± 0.0
Arg
2.615ArgAla: 2.615 ± 0.253
0.276ArgCys: 0.276 ± 0.074
1.805ArgAsp: 1.805 ± 0.152
2.523ArgGlu: 2.523 ± 0.356
1.658ArgPhe: 1.658 ± 0.179
2.781ArgGly: 2.781 ± 0.24
0.681ArgHis: 0.681 ± 0.132
2.173ArgIle: 2.173 ± 0.212
2.523ArgLys: 2.523 ± 0.357
3.37ArgLeu: 3.37 ± 0.265
1.068ArgMet: 1.068 ± 0.201
2.284ArgAsn: 2.284 ± 0.237
1.344ArgPro: 1.344 ± 0.137
1.565ArgGln: 1.565 ± 0.146
1.787ArgArg: 1.787 ± 0.254
2.376ArgSer: 2.376 ± 0.191
2.744ArgThr: 2.744 ± 0.25
3.002ArgVal: 3.002 ± 0.235
0.608ArgTrp: 0.608 ± 0.113
2.21ArgTyr: 2.21 ± 0.2
0.0ArgXaa: 0.0 ± 0.0
Ser
5.249SerAla: 5.249 ± 0.427
0.497SerCys: 0.497 ± 0.102
4.439SerAsp: 4.439 ± 0.296
3.72SerGlu: 3.72 ± 0.287
3.223SerPhe: 3.223 ± 0.375
7.68SerGly: 7.68 ± 0.617
0.81SerHis: 0.81 ± 0.116
4.807SerIle: 4.807 ± 0.48
3.905SerLys: 3.905 ± 0.352
5.102SerLeu: 5.102 ± 0.269
1.418SerMet: 1.418 ± 0.219
4.641SerAsn: 4.641 ± 0.356
2.744SerPro: 2.744 ± 0.286
2.634SerGln: 2.634 ± 0.217
2.468SerArg: 2.468 ± 0.197
6.52SerSer: 6.52 ± 0.636
5.765SerThr: 5.765 ± 0.422
4.789SerVal: 4.789 ± 0.634
0.755SerTrp: 0.755 ± 0.133
3.131SerTyr: 3.131 ± 0.201
0.0SerXaa: 0.0 ± 0.0
Thr
6.151ThrAla: 6.151 ± 0.592
0.46ThrCys: 0.46 ± 0.095
4.402ThrAsp: 4.402 ± 0.353
3.978ThrGlu: 3.978 ± 0.264
3.389ThrPhe: 3.389 ± 0.294
6.833ThrGly: 6.833 ± 0.626
0.792ThrHis: 0.792 ± 0.18
4.936ThrIle: 4.936 ± 0.415
3.536ThrLys: 3.536 ± 0.277
6.28ThrLeu: 6.28 ± 0.421
0.995ThrMet: 0.995 ± 0.13
4.236ThrAsn: 4.236 ± 0.471
3.334ThrPro: 3.334 ± 0.224
2.726ThrGln: 2.726 ± 0.224
2.505ThrArg: 2.505 ± 0.221
6.078ThrSer: 6.078 ± 0.74
6.391ThrThr: 6.391 ± 0.677
6.004ThrVal: 6.004 ± 0.645
0.663ThrTrp: 0.663 ± 0.09
2.689ThrTyr: 2.689 ± 0.232
0.0ThrXaa: 0.0 ± 0.0
Val
4.512ValAla: 4.512 ± 0.388
0.368ValCys: 0.368 ± 0.098
5.194ValAsp: 5.194 ± 0.39
4.475ValGlu: 4.475 ± 0.265
2.413ValPhe: 2.413 ± 0.228
4.77ValGly: 4.77 ± 0.454
0.645ValHis: 0.645 ± 0.1
4.254ValIle: 4.254 ± 0.337
3.702ValLys: 3.702 ± 0.294
3.776ValLeu: 3.776 ± 0.263
1.271ValMet: 1.271 ± 0.164
4.07ValAsn: 4.07 ± 0.315
3.057ValPro: 3.057 ± 0.275
2.413ValGln: 2.413 ± 0.192
2.689ValArg: 2.689 ± 0.206
5.36ValSer: 5.36 ± 0.402
5.82ValThr: 5.82 ± 0.606
4.457ValVal: 4.457 ± 0.351
0.608ValTrp: 0.608 ± 0.091
2.468ValTyr: 2.468 ± 0.248
0.0ValXaa: 0.0 ± 0.0
Trp
0.81TrpAla: 0.81 ± 0.126
0.111TrpCys: 0.111 ± 0.051
0.847TrpAsp: 0.847 ± 0.131
0.7TrpGlu: 0.7 ± 0.123
0.497TrpPhe: 0.497 ± 0.101
0.663TrpGly: 0.663 ± 0.096
0.35TrpHis: 0.35 ± 0.087
0.774TrpIle: 0.774 ± 0.152
0.902TrpLys: 0.902 ± 0.182
0.645TrpLeu: 0.645 ± 0.139
0.35TrpMet: 0.35 ± 0.08
0.718TrpAsn: 0.718 ± 0.101
0.166TrpPro: 0.166 ± 0.058
0.405TrpGln: 0.405 ± 0.089
0.387TrpArg: 0.387 ± 0.092
0.976TrpSer: 0.976 ± 0.144
0.755TrpThr: 0.755 ± 0.113
0.829TrpVal: 0.829 ± 0.122
0.184TrpTrp: 0.184 ± 0.07
0.387TrpTyr: 0.387 ± 0.069
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.394TyrAla: 2.394 ± 0.184
0.424TyrCys: 0.424 ± 0.1
3.297TyrAsp: 3.297 ± 0.298
2.597TyrGlu: 2.597 ± 0.243
1.713TyrPhe: 1.713 ± 0.187
2.707TyrGly: 2.707 ± 0.244
0.626TyrHis: 0.626 ± 0.103
2.744TyrIle: 2.744 ± 0.237
2.339TyrLys: 2.339 ± 0.221
3.315TyrLeu: 3.315 ± 0.272
0.995TyrMet: 0.995 ± 0.15
2.56TyrAsn: 2.56 ± 0.218
1.492TyrPro: 1.492 ± 0.176
1.842TyrGln: 1.842 ± 0.205
2.321TyrArg: 2.321 ± 0.227
2.855TyrSer: 2.855 ± 0.233
3.076TyrThr: 3.076 ± 0.321
3.002TyrVal: 3.002 ± 0.25
0.313TyrTrp: 0.313 ± 0.075
2.044TyrTyr: 2.044 ± 0.249
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 204 proteins (54297 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski