Aspergillus carbonarius (strain ITEM 5010)

Taxonomy: cellular organisms; Eukaryota; Opisthokonta; Fungi; Dikarya; Ascomycota; saccharomyceta; Pezizomycotina; leotiomyceta; Eurotiomycetes; Eurotiomycetidae; Eurotiales; Aspergillaceae; Aspergillus; Aspergillus carbonarius

Average proteome isoelectric point is 6.35

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 11037 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1R3RM59|A0A1R3RM59_ASPC5 Glycoside hydrolase family 28 protein OS=Aspergillus carbonarius (strain ITEM 5010) OX=602072 GN=ASPCADRAFT_507388 PE=3 SV=1
DDD2 pKa = 4.18DD3 pKa = 4.43GDDD6 pKa = 4.1DD7 pKa = 3.92GDDD10 pKa = 3.93DD11 pKa = 4.05GDDD14 pKa = 3.7DD15 pKa = 3.98GDDD18 pKa = 3.76DD19 pKa = 3.88GDDD22 pKa = 3.55DD23 pKa = 3.93GDDD26 pKa = 3.5DD27 pKa = 4.02GDDD30 pKa = 3.58DD31 pKa = 4.0ADDD34 pKa = 3.8DD35 pKa = 4.33DDD37 pKa = 5.08DD38 pKa = 4.28EE39 pKa = 6.15DDD41 pKa = 4.36DD42 pKa = 3.85EE43 pKa = 5.56GDDD46 pKa = 3.59EE47 pKa = 4.42GDDD50 pKa = 3.88NGSDDD55 pKa = 3.2GSATATPSAASSTYYY70 pKa = 10.16APAASSSAAVSNGEEE85 pKa = 4.15CSPQCTEEE93 pKa = 4.67LSNDDD98 pKa = 3.4TDDD101 pKa = 3.57SSTSFRR107 pKa = 11.84RR108 pKa = 11.84SGLFSRR114 pKa = 11.84GLTRR118 pKa = 11.84RR119 pKa = 11.84LSNYYY124 pKa = 7.07TWMLEEE130 pKa = 3.87MRR132 pKa = 11.84SGQLLAKKK140 pKa = 8.59DD141 pKa = 3.69SGMSTSEEE149 pKa = 3.99VVFGSGSTIFGQTNIRR165 pKa = 11.84GCTVVIVVSKKK176 pKa = 9.03YY177 pKa = 10.75VYYY180 pKa = 9.76AHHH183 pKa = 6.74WEEE186 pKa = 4.89PGFVTNQNGTVSFNEEE202 pKa = 3.97GFQTTVLDDD211 pKa = 5.06LTKKK215 pKa = 10.57DD216 pKa = 3.93NLKKK220 pKa = 9.27PQLTGSTAYYY230 pKa = 9.25DDD232 pKa = 4.44GTQVYYY238 pKa = 10.26GTPVDDD244 pKa = 3.52TGQGAKKK251 pKa = 9.92YY252 pKa = 9.77SQISQVTATINSVLGLSTDDD272 pKa = 3.24SEEE275 pKa = 4.54YY276 pKa = 10.66YY277 pKa = 9.05YY278 pKa = 8.54TTSTDDD284 pKa = 3.39QGKKK288 pKa = 8.09LFTYYY293 pKa = 10.15ANTCNKKK300 pKa = 9.37VWQVWYYY307 pKa = 10.83DDD309 pKa = 3.71SSNALWSSSEEE320 pKa = 4.14SSCAASASITPSRR333 pKa = 11.84TAALPAYYY341 pKa = 8.6EEE343 pKa = 4.16STFRR347 pKa = 11.84VLPKKK352 pKa = 8.18TPSY

Molecular weight: 37.48 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1R3RG69|A0A1R3RG69_ASPC5 Uncharacterized protein OS=Aspergillus carbonarius (strain ITEM 5010) OX=602072 GN=ASPCADRAFT_7695 PE=4 SV=1
MM1 pKa = 7.18YY2 pKa = 10.64GRR4 pKa = 11.84VANQGSRR11 pKa = 11.84SGRR14 pKa = 11.84PPSIHH19 pKa = 6.3QSSAAAAVIRR29 pKa = 11.84RR30 pKa = 11.84RR31 pKa = 11.84VNFPRR36 pKa = 11.84SQAMRR41 pKa = 11.84IKK43 pKa = 10.44RR44 pKa = 11.84ALPGIGFRR52 pKa = 11.84ASPPP56 pKa = 3.38

Molecular weight: 6.09 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

11034

3

11037

5099753

42

9610

462.1

51.2

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.449 ± 0.02

1.318 ± 0.008

5.587 ± 0.018

5.941 ± 0.02

3.825 ± 0.015

6.888 ± 0.02

2.498 ± 0.011

5.075 ± 0.016

4.239 ± 0.021

9.391 ± 0.025

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.203 ± 0.008

3.504 ± 0.011

5.952 ± 0.02

3.963 ± 0.014

6.125 ± 0.019

8.125 ± 0.028

6.028 ± 0.014

6.378 ± 0.017

1.552 ± 0.009

2.957 ± 0.013
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski