Salisediminibacterium haloalkalitolerans

Taxonomy: cellular organisms; Bacteria; Terrabacteria group; Firmicutes; Bacilli; Bacillales; Bacillaceae; Salisediminibacterium

Average proteome isoelectric point is 5.69

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 2769 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A1H9VJ15|A0A1H9VJ15_9BACI Uncharacterized protein OS=Salisediminibacterium haloalkalitolerans OX=1464123 GN=SAMN05444126_12118 PE=4 SV=1
MM1 pKa = 7.53NIYY4 pKa = 9.76GKK6 pKa = 10.15KK7 pKa = 9.71VLVAGSLLLLAACGNDD23 pKa = 3.37EE24 pKa = 4.15NEE26 pKa = 3.99EE27 pKa = 3.76LAAEE31 pKa = 4.2NEE33 pKa = 3.79ADD35 pKa = 3.7EE36 pKa = 4.42NNRR39 pKa = 11.84GILSDD44 pKa = 4.35DD45 pKa = 4.05NEE47 pKa = 4.34NLADD51 pKa = 4.65DD52 pKa = 4.87NNEE55 pKa = 3.66QTEE58 pKa = 4.17NSDD61 pKa = 3.62SKK63 pKa = 11.52EE64 pKa = 4.05NAPEE68 pKa = 4.38PDD70 pKa = 3.45PEE72 pKa = 4.12EE73 pKa = 4.44NIGNFNGNLAQGGRR87 pKa = 11.84QAYY90 pKa = 9.66HH91 pKa = 6.64EE92 pKa = 4.38DD93 pKa = 3.4VFYY96 pKa = 11.43VSAALDD102 pKa = 3.94EE103 pKa = 4.27EE104 pKa = 4.5HH105 pKa = 7.24GIYY108 pKa = 10.36AVPLEE113 pKa = 4.34TNEE116 pKa = 3.78YY117 pKa = 9.69EE118 pKa = 4.31QLTDD122 pKa = 4.37GKK124 pKa = 10.11AQDD127 pKa = 3.43MHH129 pKa = 5.96VTDD132 pKa = 4.78DD133 pKa = 4.3GIYY136 pKa = 9.48YY137 pKa = 8.83LTDD140 pKa = 3.73PGYY143 pKa = 11.13DD144 pKa = 3.41NDD146 pKa = 3.8SDD148 pKa = 4.24EE149 pKa = 4.96IKK151 pKa = 10.38LIHH154 pKa = 6.25YY155 pKa = 10.23AFDD158 pKa = 3.72TDD160 pKa = 3.82EE161 pKa = 5.51KK162 pKa = 10.73NTLDD166 pKa = 4.02EE167 pKa = 4.81GNLRR171 pKa = 11.84NLQMNHH177 pKa = 6.89DD178 pKa = 4.22GLWYY182 pKa = 10.49LKK184 pKa = 10.45AGNEE188 pKa = 3.87GGMNFINKK196 pKa = 9.07NMEE199 pKa = 3.83TGEE202 pKa = 4.0FDD204 pKa = 3.66EE205 pKa = 5.54FSSGQSQIIIDD216 pKa = 4.06GDD218 pKa = 3.91DD219 pKa = 4.74AISQAEE225 pKa = 4.17TFIYY229 pKa = 10.92DD230 pKa = 3.14MDD232 pKa = 5.65GPDD235 pKa = 3.57GTIEE239 pKa = 4.33DD240 pKa = 4.17EE241 pKa = 4.35PVVEE245 pKa = 4.33NATPPVILKK254 pKa = 10.59DD255 pKa = 3.26RR256 pKa = 11.84VVYY259 pKa = 10.55YY260 pKa = 10.3NGPNGIEE267 pKa = 3.94AHH269 pKa = 6.79DD270 pKa = 4.83LDD272 pKa = 4.47SGQTIKK278 pKa = 9.41ITNEE282 pKa = 3.49RR283 pKa = 11.84VSDD286 pKa = 3.74FNVRR290 pKa = 11.84NGIVYY295 pKa = 7.33FTPPFQSEE303 pKa = 4.23AARR306 pKa = 11.84EE307 pKa = 4.26SYY309 pKa = 10.52RR310 pKa = 11.84VDD312 pKa = 3.28ADD314 pKa = 3.52GTNEE318 pKa = 3.86EE319 pKa = 4.15NLEE322 pKa = 4.58GISYY326 pKa = 8.5TYY328 pKa = 11.81YY329 pKa = 10.23MFDD332 pKa = 3.58DD333 pKa = 4.67FAVHH337 pKa = 5.85SSGRR341 pKa = 11.84HH342 pKa = 3.74GVILYY347 pKa = 10.55SKK349 pKa = 8.7ITYY352 pKa = 10.09DD353 pKa = 3.42SEE355 pKa = 4.22EE356 pKa = 4.25SEE358 pKa = 4.33KK359 pKa = 10.34IYY361 pKa = 9.57YY362 pKa = 7.41TNPGMEE368 pKa = 4.44DD369 pKa = 2.91

Molecular weight: 41.24 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A1H9WPY8|A0A1H9WPY8_9BACI Sucrose-6-phosphate hydrolase OS=Salisediminibacterium haloalkalitolerans OX=1464123 GN=SAMN05444126_1424 PE=3 SV=1
MM1 pKa = 7.51SKK3 pKa = 9.27ATFQPNNRR11 pKa = 11.84KK12 pKa = 9.23RR13 pKa = 11.84KK14 pKa = 8.22KK15 pKa = 8.69VHH17 pKa = 5.45GFRR20 pKa = 11.84ARR22 pKa = 11.84MATKK26 pKa = 10.29NGRR29 pKa = 11.84QVLQRR34 pKa = 11.84RR35 pKa = 11.84RR36 pKa = 11.84RR37 pKa = 11.84KK38 pKa = 8.93GRR40 pKa = 11.84KK41 pKa = 8.7VLSAA45 pKa = 4.05

Molecular weight: 5.35 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

2769

0

2769

857196

27

3116

309.6

34.52

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.482 ± 0.054

0.623 ± 0.014

6.019 ± 0.056

8.353 ± 0.07

4.322 ± 0.04

7.172 ± 0.045

2.205 ± 0.025

6.598 ± 0.043

5.211 ± 0.049

9.256 ± 0.054

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.93 ± 0.024

3.914 ± 0.034

3.816 ± 0.024

3.721 ± 0.033

4.658 ± 0.035

5.778 ± 0.033

5.575 ± 0.03

7.057 ± 0.04

1.0 ± 0.016

3.307 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski