Marinitenerispora sediminis 
Average proteome isoelectric point is 6.5 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 5230 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A368T6X3|A0A368T6X3_9ACTN AsnC family transcriptional regulator OS=Marinitenerispora sediminis OX=1931232 GN=DEF24_09175 PE=4 SV=1MM1 pKa = 7.85  RR2 pKa = 11.84  SHH4 pKa = 6.81  PVGRR8 pKa = 11.84  VGAAAAAGTCLALLLTACGSGYY30 pKa = 10.99  GDD32 pKa = 3.49  TPEE35 pKa = 5.61  GDD37 pKa = 3.47  GALTVLFGSSGEE49 pKa = 4.22  AEE51 pKa = 4.09  TAAVKK56 pKa = 10.25  AALAAWSEE64 pKa = 4.26  EE65 pKa = 4.04  SGVPAEE71 pKa = 5.3  AIPAQDD77 pKa = 4.03  LVQQLRR83 pKa = 11.84  QGFAGGDD90 pKa = 3.67  PADD93 pKa = 3.75  VFYY96 pKa = 11.32  VSPDD100 pKa = 3.16  LFQQYY105 pKa = 11.21  AEE107 pKa = 4.72  GGSLYY112 pKa = 10.06  PYY114 pKa = 10.09  GDD116 pKa = 3.65  QIEE119 pKa = 4.91  DD120 pKa = 3.23  VDD122 pKa = 4.36  DD123 pKa = 4.97  FYY125 pKa = 11.2  PALRR129 pKa = 11.84  DD130 pKa = 3.32  SYY132 pKa = 10.66  TYY134 pKa = 10.79  EE135 pKa = 4.68  DD136 pKa = 4.1  EE137 pKa = 4.94  LYY139 pKa = 10.67  CVPKK143 pKa = 10.46  DD144 pKa = 4.07  YY145 pKa = 9.12  NTHH148 pKa = 6.22  ALVINTDD155 pKa = 2.96  LWEE158 pKa = 4.2  EE159 pKa = 4.21  AGLGDD164 pKa = 5.71  DD165 pKa = 6.16  DD166 pKa = 6.2  IPTSWEE172 pKa = 3.81  EE173 pKa = 3.71  LAEE176 pKa = 4.06  TAEE179 pKa = 4.06  QLTEE183 pKa = 3.86  GDD185 pKa = 3.2  RR186 pKa = 11.84  VGFALNGDD194 pKa = 4.08  YY195 pKa = 11.29  NSAGTFMLQAGGWFVNDD212 pKa = 4.73  DD213 pKa = 3.61  NTEE216 pKa = 4.11  ATGDD220 pKa = 3.74  TPEE223 pKa = 4.38  NLAALDD229 pKa = 3.96  YY230 pKa = 9.56  LTTGLDD236 pKa = 2.74  EE237 pKa = 5.53  GYY239 pKa = 10.7  FSFVKK244 pKa = 10.73  DD245 pKa = 2.97  IDD247 pKa = 3.9  AQSGSEE253 pKa = 3.71  ALGRR257 pKa = 11.84  GRR259 pKa = 11.84  AAMVMDD265 pKa = 4.75  GGWVTGAFDD274 pKa = 4.18  NDD276 pKa = 3.63  YY277 pKa = 11.47  PEE279 pKa = 4.89  LNWRR283 pKa = 11.84  AVEE286 pKa = 4.02  LPEE289 pKa = 5.22  GPGGQATTVFSNCWGIAEE307 pKa = 4.4  ASGDD311 pKa = 3.59  HH312 pKa = 6.28  EE313 pKa = 4.35  AAVDD317 pKa = 3.74  LVRR320 pKa = 11.84  YY321 pKa = 7.33  LTSPEE326 pKa = 3.82  QQQAFAEE333 pKa = 4.47  DD334 pKa = 4.72  FGAVPPRR341 pKa = 11.84  EE342 pKa = 4.15  SLADD346 pKa = 3.29  WTAEE350 pKa = 4.05  TFPEE354 pKa = 4.29  KK355 pKa = 10.54  AAFAAGVDD363 pKa = 3.92  YY364 pKa = 11.26  ARR366 pKa = 11.84  GQVPVPGFMSVLNEE380 pKa = 4.15  FTTGMQGVAAGSAEE394 pKa = 4.07  PADD397 pKa = 4.13  VLAQLQRR404 pKa = 11.84  DD405 pKa = 4.01  GEE407 pKa = 4.35  EE408 pKa = 4.24  ALGEE412 pKa = 4.12  
 43.56 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  3.733 
IPC2_protein 3.643 
IPC_protein 3.656 
Toseland    3.452 
ProMoST     3.795 
Dawson      3.643 
Bjellqvist  3.808 
Wikipedia   3.554 
Rodwell     3.49 
Grimsley    3.35 
Solomon     3.63 
Lehninger   3.592 
Nozaki      3.745 
DTASelect   3.961 
Thurlkill   3.49 
EMBOSS      3.567 
Sillero     3.783 
Patrickios  0.82 
IPC_peptide 3.63 
IPC2_peptide  3.757 
IPC2.peptide.svr19  3.697 
 Protein with the highest isoelectric point: 
>tr|A0A368T1V2|A0A368T1V2_9ACTN Aldo/keto reductase OS=Marinitenerispora sediminis OX=1931232 GN=DEF24_19590 PE=4 SV=1MM1 pKa = 7.78  RR2 pKa = 11.84  ALIAPPASASALSARR17 pKa = 11.84  AARR20 pKa = 11.84  VATLLRR26 pKa = 11.84  VSATRR31 pKa = 11.84  TSLTARR37 pKa = 11.84  ARR39 pKa = 11.84  SRR41 pKa = 11.84  STSSRR46 pKa = 11.84  LGVPPATPARR56 pKa = 11.84  LSAAPALAALIRR68 pKa = 11.84  VLKK71 pKa = 10.53  RR72 pKa = 11.84  WTTT75 pKa = 3.31  
 7.76 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  9.526 
IPC2_protein 11.272 
IPC_protein 12.881 
Toseland    13.042 
ProMoST     13.539 
Dawson      13.042 
Bjellqvist  13.042 
Wikipedia   13.525 
Rodwell     12.574 
Grimsley    13.086 
Solomon     13.539 
Lehninger   13.437 
Nozaki      13.042 
DTASelect   13.042 
Thurlkill   13.042 
EMBOSS      13.539 
Sillero     13.042 
Patrickios  12.31 
IPC_peptide 13.539 
IPC2_peptide  12.53 
IPC2.peptide.svr19  9.231 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        5230 
0
5230 
1586353
22
3991
303.3
32.43
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        14.433 ± 0.057
0.748 ± 0.009
6.043 ± 0.034
5.977 ± 0.036
2.649 ± 0.021
9.597 ± 0.033
2.223 ± 0.017
3.081 ± 0.027
1.31 ± 0.023
10.354 ± 0.047
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        1.693 ± 0.014
1.572 ± 0.015
6.347 ± 0.035
2.415 ± 0.022
9.124 ± 0.044
4.684 ± 0.024
5.488 ± 0.025
8.828 ± 0.032
1.494 ± 0.015
1.939 ± 0.017
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here