Marinitenerispora sediminis
Average proteome isoelectric point is 6.5
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5230 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A368T6X3|A0A368T6X3_9ACTN AsnC family transcriptional regulator OS=Marinitenerispora sediminis OX=1931232 GN=DEF24_09175 PE=4 SV=1
MM1 pKa = 7.85 RR2 pKa = 11.84 SHH4 pKa = 6.81 PVGRR8 pKa = 11.84 VGAAAAAGTCLALLLTACGSGYY30 pKa = 10.99 GDD32 pKa = 3.49 TPEE35 pKa = 5.61 GDD37 pKa = 3.47 GALTVLFGSSGEE49 pKa = 4.22 AEE51 pKa = 4.09 TAAVKK56 pKa = 10.25 AALAAWSEE64 pKa = 4.26 EE65 pKa = 4.04 SGVPAEE71 pKa = 5.3 AIPAQDD77 pKa = 4.03 LVQQLRR83 pKa = 11.84 QGFAGGDD90 pKa = 3.67 PADD93 pKa = 3.75 VFYY96 pKa = 11.32 VSPDD100 pKa = 3.16 LFQQYY105 pKa = 11.21 AEE107 pKa = 4.72 GGSLYY112 pKa = 10.06 PYY114 pKa = 10.09 GDD116 pKa = 3.65 QIEE119 pKa = 4.91 DD120 pKa = 3.23 VDD122 pKa = 4.36 DD123 pKa = 4.97 FYY125 pKa = 11.2 PALRR129 pKa = 11.84 DD130 pKa = 3.32 SYY132 pKa = 10.66 TYY134 pKa = 10.79 EE135 pKa = 4.68 DD136 pKa = 4.1 EE137 pKa = 4.94 LYY139 pKa = 10.67 CVPKK143 pKa = 10.46 DD144 pKa = 4.07 YY145 pKa = 9.12 NTHH148 pKa = 6.22 ALVINTDD155 pKa = 2.96 LWEE158 pKa = 4.2 EE159 pKa = 4.21 AGLGDD164 pKa = 5.71 DD165 pKa = 6.16 DD166 pKa = 6.2 IPTSWEE172 pKa = 3.81 EE173 pKa = 3.71 LAEE176 pKa = 4.06 TAEE179 pKa = 4.06 QLTEE183 pKa = 3.86 GDD185 pKa = 3.2 RR186 pKa = 11.84 VGFALNGDD194 pKa = 4.08 YY195 pKa = 11.29 NSAGTFMLQAGGWFVNDD212 pKa = 4.73 DD213 pKa = 3.61 NTEE216 pKa = 4.11 ATGDD220 pKa = 3.74 TPEE223 pKa = 4.38 NLAALDD229 pKa = 3.96 YY230 pKa = 9.56 LTTGLDD236 pKa = 2.74 EE237 pKa = 5.53 GYY239 pKa = 10.7 FSFVKK244 pKa = 10.73 DD245 pKa = 2.97 IDD247 pKa = 3.9 AQSGSEE253 pKa = 3.71 ALGRR257 pKa = 11.84 GRR259 pKa = 11.84 AAMVMDD265 pKa = 4.75 GGWVTGAFDD274 pKa = 4.18 NDD276 pKa = 3.63 YY277 pKa = 11.47 PEE279 pKa = 4.89 LNWRR283 pKa = 11.84 AVEE286 pKa = 4.02 LPEE289 pKa = 5.22 GPGGQATTVFSNCWGIAEE307 pKa = 4.4 ASGDD311 pKa = 3.59 HH312 pKa = 6.28 EE313 pKa = 4.35 AAVDD317 pKa = 3.74 LVRR320 pKa = 11.84 YY321 pKa = 7.33 LTSPEE326 pKa = 3.82 QQQAFAEE333 pKa = 4.47 DD334 pKa = 4.72 FGAVPPRR341 pKa = 11.84 EE342 pKa = 4.15 SLADD346 pKa = 3.29 WTAEE350 pKa = 4.05 TFPEE354 pKa = 4.29 KK355 pKa = 10.54 AAFAAGVDD363 pKa = 3.92 YY364 pKa = 11.26 ARR366 pKa = 11.84 GQVPVPGFMSVLNEE380 pKa = 4.15 FTTGMQGVAAGSAEE394 pKa = 4.07 PADD397 pKa = 4.13 VLAQLQRR404 pKa = 11.84 DD405 pKa = 4.01 GEE407 pKa = 4.35 EE408 pKa = 4.24 ALGEE412 pKa = 4.12
Molecular weight: 43.56 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.733
IPC2_protein 3.643
IPC_protein 3.656
Toseland 3.452
ProMoST 3.795
Dawson 3.643
Bjellqvist 3.808
Wikipedia 3.554
Rodwell 3.49
Grimsley 3.35
Solomon 3.63
Lehninger 3.592
Nozaki 3.745
DTASelect 3.961
Thurlkill 3.49
EMBOSS 3.567
Sillero 3.783
Patrickios 0.82
IPC_peptide 3.63
IPC2_peptide 3.757
IPC2.peptide.svr19 3.697
Protein with the highest isoelectric point:
>tr|A0A368T1V2|A0A368T1V2_9ACTN Aldo/keto reductase OS=Marinitenerispora sediminis OX=1931232 GN=DEF24_19590 PE=4 SV=1
MM1 pKa = 7.78 RR2 pKa = 11.84 ALIAPPASASALSARR17 pKa = 11.84 AARR20 pKa = 11.84 VATLLRR26 pKa = 11.84 VSATRR31 pKa = 11.84 TSLTARR37 pKa = 11.84 ARR39 pKa = 11.84 SRR41 pKa = 11.84 STSSRR46 pKa = 11.84 LGVPPATPARR56 pKa = 11.84 LSAAPALAALIRR68 pKa = 11.84 VLKK71 pKa = 10.53 RR72 pKa = 11.84 WTTT75 pKa = 3.31
Molecular weight: 7.76 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.526
IPC2_protein 11.272
IPC_protein 12.881
Toseland 13.042
ProMoST 13.539
Dawson 13.042
Bjellqvist 13.042
Wikipedia 13.525
Rodwell 12.574
Grimsley 13.086
Solomon 13.539
Lehninger 13.437
Nozaki 13.042
DTASelect 13.042
Thurlkill 13.042
EMBOSS 13.539
Sillero 13.042
Patrickios 12.31
IPC_peptide 13.539
IPC2_peptide 12.53
IPC2.peptide.svr19 9.231
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5230
0
5230
1586353
22
3991
303.3
32.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.433 ± 0.057
0.748 ± 0.009
6.043 ± 0.034
5.977 ± 0.036
2.649 ± 0.021
9.597 ± 0.033
2.223 ± 0.017
3.081 ± 0.027
1.31 ± 0.023
10.354 ± 0.047
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.693 ± 0.014
1.572 ± 0.015
6.347 ± 0.035
2.415 ± 0.022
9.124 ± 0.044
4.684 ± 0.024
5.488 ± 0.025
8.828 ± 0.032
1.494 ± 0.015
1.939 ± 0.017
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here