Serinibacter arcticus
Average proteome isoelectric point is 6.0
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 2999 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4Z1DXW7|A0A4Z1DXW7_9MICO Phosphate starvation-inducible protein PhoH predicted ATPase OS=Serinibacter arcticus OX=1655435 GN=SERN_1969 PE=4 SV=1
MM1 pKa = 7.39 AVVGLAASSLVLAGCSSEE19 pKa = 4.22 RR20 pKa = 11.84 GEE22 pKa = 4.49 GGSDD26 pKa = 3.2 GSSSEE31 pKa = 4.25 GGSGTSSEE39 pKa = 4.35 TGEE42 pKa = 4.16 AAGPTTFTFASSSDD56 pKa = 3.77 PASLDD61 pKa = 3.26 PAFASDD67 pKa = 3.92 GEE69 pKa = 4.5 SFRR72 pKa = 11.84 VARR75 pKa = 11.84 QMFEE79 pKa = 3.81 GLVGVEE85 pKa = 4.84 PGTADD90 pKa = 3.86 PAPLLAEE97 pKa = 4.14 SWDD100 pKa = 3.68 VSDD103 pKa = 6.37 DD104 pKa = 3.4 GLEE107 pKa = 3.93 YY108 pKa = 10.56 TFQLKK113 pKa = 10.46 EE114 pKa = 4.0 GVSFHH119 pKa = 7.64 DD120 pKa = 3.56 GTPFNGDD127 pKa = 3.19 AVCFNFDD134 pKa = 2.93 RR135 pKa = 11.84 WDD137 pKa = 3.6 GFTGIAASEE146 pKa = 4.2 SMSYY150 pKa = 10.97 YY151 pKa = 8.36 WGKK154 pKa = 10.82 VNGGYY159 pKa = 10.35 EE160 pKa = 4.2 GAGKK164 pKa = 10.03 YY165 pKa = 9.85 ASCEE169 pKa = 4.07 APDD172 pKa = 4.39 DD173 pKa = 3.76 ATAVITLSSPLPEE186 pKa = 4.01 LVAALSLPAFSMQSPTALAEE206 pKa = 4.01 YY207 pKa = 10.27 GADD210 pKa = 3.12 EE211 pKa = 4.51 VGGSEE216 pKa = 5.19 DD217 pKa = 4.66 APTLPEE223 pKa = 4.16 YY224 pKa = 10.39 ATDD227 pKa = 3.97 HH228 pKa = 6.1 PTGTGPFTFEE238 pKa = 3.69 SWSPGEE244 pKa = 4.15 NVTLAANTEE253 pKa = 4.13 YY254 pKa = 9.94 WGEE257 pKa = 3.96 QGQITTVIFPVISDD271 pKa = 3.56 ATARR275 pKa = 11.84 RR276 pKa = 11.84 QALEE280 pKa = 4.12 AGDD283 pKa = 3.54 IDD285 pKa = 5.63 GYY287 pKa = 11.33 DD288 pKa = 3.43 LVGPADD294 pKa = 3.76 VVALEE299 pKa = 4.21 DD300 pKa = 3.2 AGYY303 pKa = 8.83 TIEE306 pKa = 5.42 NRR308 pKa = 11.84 DD309 pKa = 3.96 PFNVLYY315 pKa = 10.85 LGMNQAVPEE324 pKa = 4.19 LADD327 pKa = 3.07 IRR329 pKa = 11.84 VRR331 pKa = 11.84 QAIAHH336 pKa = 6.96 AIDD339 pKa = 3.84 KK340 pKa = 9.98 DD341 pKa = 3.83 ALVAATLPEE350 pKa = 4.32 GTLVATNFVPPSVRR364 pKa = 11.84 GHH366 pKa = 6.2 SDD368 pKa = 3.39 DD369 pKa = 3.83 VPTYY373 pKa = 11.21 DD374 pKa = 4.58 FDD376 pKa = 3.95 QDD378 pKa = 3.37 EE379 pKa = 4.61 ARR381 pKa = 11.84 SLLAEE386 pKa = 4.75 AGVSDD391 pKa = 3.91 LTLNFTYY398 pKa = 7.79 PTNVSRR404 pKa = 11.84 PYY406 pKa = 9.99 MPTPEE411 pKa = 4.04 QVFEE415 pKa = 4.97 RR416 pKa = 11.84 IAADD420 pKa = 3.64 LEE422 pKa = 4.56 EE423 pKa = 4.36 VGITVEE429 pKa = 4.16 ATPLPWSPDD438 pKa = 3.31 YY439 pKa = 11.15 LDD441 pKa = 5.65 RR442 pKa = 11.84 IQGGADD448 pKa = 3.12 HH449 pKa = 7.02 GLHH452 pKa = 6.13 LLGWTGDD459 pKa = 3.72 YY460 pKa = 11.07 NDD462 pKa = 4.18 TYY464 pKa = 11.73 NFIGVFFGAQSNEE477 pKa = 3.57 WGFDD481 pKa = 3.45 DD482 pKa = 4.47 PALFEE487 pKa = 4.47 SLSTARR493 pKa = 11.84 VATDD497 pKa = 3.22 EE498 pKa = 4.59 AEE500 pKa = 4.11 QTSLYY505 pKa = 10.79 EE506 pKa = 3.99 EE507 pKa = 4.8 ANVQIMEE514 pKa = 4.55 NLPGIPIAHH523 pKa = 7.04 PVPSLAFRR531 pKa = 11.84 PGVEE535 pKa = 4.66 NYY537 pKa = 7.8 PASPVQDD544 pKa = 3.03 EE545 pKa = 5.16 VYY547 pKa = 11.09 NVITLTDD554 pKa = 3.21
Molecular weight: 58.69 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.701
IPC2_protein 3.745
IPC_protein 3.757
Toseland 3.541
ProMoST 3.884
Dawson 3.732
Bjellqvist 3.897
Wikipedia 3.643
Rodwell 3.579
Grimsley 3.452
Solomon 3.732
Lehninger 3.681
Nozaki 3.834
DTASelect 4.05
Thurlkill 3.579
EMBOSS 3.656
Sillero 3.872
Patrickios 1.125
IPC_peptide 3.732
IPC2_peptide 3.859
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A4Z1E3U8|A0A4Z1E3U8_9MICO Putative lipoprotein OS=Serinibacter arcticus OX=1655435 GN=SERN_1446 PE=4 SV=1
MM1 pKa = 7.56 AVPKK5 pKa = 10.48 RR6 pKa = 11.84 KK7 pKa = 9.07 MSRR10 pKa = 11.84 SNTRR14 pKa = 11.84 ARR16 pKa = 11.84 RR17 pKa = 11.84 SQWKK21 pKa = 8.13 ATPATLSTCSKK32 pKa = 10.21 CKK34 pKa = 10.33 APRR37 pKa = 11.84 LSHH40 pKa = 5.71 QACPSCGAYY49 pKa = 9.97 GEE51 pKa = 4.24 RR52 pKa = 11.84 TYY54 pKa = 11.79 AEE56 pKa = 5.25 AIRR59 pKa = 11.84 SDD61 pKa = 3.24 AA62 pKa = 4.14
Molecular weight: 6.83 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.343
IPC2_protein 9.604
IPC_protein 10.131
Toseland 10.584
ProMoST 10.218
Dawson 10.687
Bjellqvist 10.394
Wikipedia 10.862
Rodwell 11.023
Grimsley 10.73
Solomon 10.774
Lehninger 10.76
Nozaki 10.628
DTASelect 10.365
Thurlkill 10.584
EMBOSS 10.979
Sillero 10.628
Patrickios 10.804
IPC_peptide 10.789
IPC2_peptide 9.677
IPC2.peptide.svr19 8.499
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
2999
0
2999
1041505
37
3780
347.3
36.68
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
14.299 ± 0.062
0.484 ± 0.009
6.436 ± 0.038
5.484 ± 0.039
2.547 ± 0.029
9.567 ± 0.042
1.987 ± 0.025
3.366 ± 0.031
1.253 ± 0.023
10.172 ± 0.057
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.568 ± 0.016
1.582 ± 0.028
5.892 ± 0.042
2.562 ± 0.021
7.518 ± 0.061
5.418 ± 0.03
6.698 ± 0.058
9.926 ± 0.049
1.48 ± 0.019
1.764 ± 0.021
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here