Planctomycetes bacterium Pan189

Taxonomy: cellular organisms; Bacteria; PVC group; Planctomycetes; unclassified Planctomycetes

Average proteome isoelectric point is 5.98

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 4295 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A517R6B1|A0A517R6B1_9BACT Uncharacterized protein OS=Planctomycetes bacterium Pan189 OX=2527980 GN=Pan189_38160 PE=4 SV=1
MM1 pKa = 7.64RR2 pKa = 11.84ALATLLATLLSLCLGCSPNSDD23 pKa = 3.63APGVAPSSTTPPSTAADD40 pKa = 3.87PGAVGWVTITNNNYY54 pKa = 10.17GSMGSEE60 pKa = 3.91TGTAPDD66 pKa = 3.97GSDD69 pKa = 2.91TYY71 pKa = 10.77KK72 pKa = 10.56YY73 pKa = 10.5KK74 pKa = 10.63VVRR77 pKa = 11.84AHH79 pKa = 7.14FGDD82 pKa = 4.19LLQDD86 pKa = 3.16IAEE89 pKa = 4.31TTEE92 pKa = 4.19TSVSCSDD99 pKa = 3.22ATKK102 pKa = 10.96LDD104 pKa = 3.43MGVTLTFEE112 pKa = 4.48GATAADD118 pKa = 4.43LVADD122 pKa = 5.26LAGQLNLAVSEE133 pKa = 4.46DD134 pKa = 3.76PPGNFVLSSQQ144 pKa = 3.58

Molecular weight: 14.6 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A517R596|A0A517R596_9BACT Anaphase-promoting complex cyclosome subunit 3 OS=Planctomycetes bacterium Pan189 OX=2527980 GN=Pan189_34680 PE=4 SV=1
MM1 pKa = 7.83AKK3 pKa = 8.26TQRR6 pKa = 11.84KK7 pKa = 8.43LKK9 pKa = 9.88KK10 pKa = 9.26ANHH13 pKa = 6.11GKK15 pKa = 10.09RR16 pKa = 11.84PASAQARR23 pKa = 11.84RR24 pKa = 11.84SKK26 pKa = 10.25RR27 pKa = 11.84AKK29 pKa = 10.13IKK31 pKa = 9.46TT32 pKa = 3.51

Molecular weight: 3.65 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

4295

0

4295

1510845

29

9367

351.8

38.7

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

9.889 ± 0.053

1.068 ± 0.017

6.417 ± 0.039

6.856 ± 0.041

3.906 ± 0.023

7.918 ± 0.063

2.025 ± 0.022

5.11 ± 0.029

3.564 ± 0.039

9.595 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

1.968 ± 0.02

2.939 ± 0.03

5.239 ± 0.039

3.33 ± 0.022

7.229 ± 0.054

6.464 ± 0.037

5.47 ± 0.054

7.291 ± 0.035

1.367 ± 0.017

2.354 ± 0.025
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski