Capybara microvirus Cap1_SP_107 
Average proteome isoelectric point is 5.95 
Get precalculated fractions of proteins 
 
  
    Acidic  
 
  
    pI < 6.8  
 
  
    6.8-7.4  
 
  
    pI > 7.4  
 
  
    Basic  
    
 
  
    All  
 
Note: above files contain also dissociation constants (pKa) 
Virtual 2D-PAGE plot for 4 proteins (isoelectric point calculated using IPC2_protein) 
 
Get csv file with sequences according to given criteria: 
* You can choose from 21 different methods for calculating isoelectric point 
 Summary statistics related to proteome-wise predictions 
Protein with the lowest isoelectric point: 
>tr|A0A4V1FVN8|A0A4V1FVN8_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_107 OX=2584805 PE=4 SV=1MM1 pKa = 7.44  KK2 pKa = 9.74  VTSKK6 pKa = 10.62  LYY8 pKa = 10.57  KK9 pKa = 8.79  VGPWFSQPQDD19 pKa = 3.88  DD20 pKa = 3.74  IRR22 pKa = 11.84  VVVAYY27 pKa = 10.36  DD28 pKa = 3.48  EE29 pKa = 5.89  DD30 pKa = 4.48  GNQYY34 pKa = 7.61  TTFQPYY40 pKa = 10.34  EE41 pKa = 4.16  GDD43 pKa = 3.3  PDD45 pKa = 3.81  KK46 pKa = 11.35  FGTADD51 pKa = 3.96  DD52 pKa = 4.13  WSLSNLMAAGIDD64 pKa = 3.37  PASFNTTYY72 pKa = 11.2  SPLTRR77 pKa = 11.84  LDD79 pKa = 3.32  SAAEE83 pKa = 3.94  FAQISSVAEE92 pKa = 3.83  ALIDD96 pKa = 3.94  EE97 pKa = 5.15  LDD99 pKa = 3.55  AAAAEE104 pKa = 4.48  PQVSMEE110 pKa = 4.76  DD111 pKa = 3.48  KK112 pKa = 10.96  IEE114 pKa = 3.79  
 12.52 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  4.159 
IPC2_protein 3.795 
IPC_protein 3.757 
Toseland    3.541 
ProMoST     3.897 
Dawson      3.757 
Bjellqvist  3.961 
Wikipedia   3.706 
Rodwell     3.592 
Grimsley    3.452 
Solomon     3.745 
Lehninger   3.694 
Nozaki      3.884 
DTASelect   4.113 
Thurlkill   3.605 
EMBOSS      3.706 
Sillero     3.884 
Patrickios  3.401 
IPC_peptide 3.732 
IPC2_peptide  3.859 
IPC2.peptide.svr19  3.848 
 Protein with the highest isoelectric point: 
>tr|A0A4P8W7S1|A0A4P8W7S1_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_107 OX=2584805 PE=3 SV=1MM1 pKa = 7.71  KK2 pKa = 9.9  STITFTNPTTYY13 pKa = 10.47  FHH15 pKa = 7.68  PDD17 pKa = 3.06  YY18 pKa = 10.85  SAGSVTIQNPALYY31 pKa = 10.24  FSDD34 pKa = 3.37  TTKK37 pKa = 10.05  QQGYY41 pKa = 5.41  EE42 pKa = 3.64  TRR44 pKa = 11.84  LYY46 pKa = 10.78  FEE48 pKa = 4.62  YY49 pKa = 10.53  EE50 pKa = 3.84  GTKK53 pKa = 10.7  AKK55 pKa = 10.89  GGDD58 pKa = 3.48  SYY60 pKa = 11.65  FYY62 pKa = 10.75  TLTYY66 pKa = 11.07  NNAALPHH73 pKa = 6.55  YY74 pKa = 10.06  IPALNSVVRR83 pKa = 11.84  GSQLGGIPCFDD94 pKa = 4.13  YY95 pKa = 11.43  EE96 pKa = 4.63  DD97 pKa = 3.33  IRR99 pKa = 11.84  YY100 pKa = 7.04  ITHH103 pKa = 6.78  GGLDD107 pKa = 3.36  KK108 pKa = 11.1  KK109 pKa = 10.59  LLRR112 pKa = 11.84  NFGTKK117 pKa = 8.06  MKK119 pKa = 10.51  YY120 pKa = 9.86  FVGCEE125 pKa = 3.73  LGEE128 pKa = 4.51  GKK130 pKa = 10.54  GSRR133 pKa = 11.84  GAGNNPHH140 pKa = 5.57  YY141 pKa = 10.52  HH142 pKa = 5.6  VLFFLTNAEE151 pKa = 4.14  DD152 pKa = 3.33  EE153 pKa = 4.58  RR154 pKa = 11.84  YY155 pKa = 8.55  PYY157 pKa = 10.65  HH158 pKa = 7.55  RR159 pKa = 11.84  ILPEE163 pKa = 3.75  QFEE166 pKa = 4.68  TLIKK170 pKa = 9.83  TYY172 pKa = 9.21  WQGTSEE178 pKa = 4.65  KK179 pKa = 9.53  IDD181 pKa = 3.37  YY182 pKa = 10.58  SKK184 pKa = 11.64  AKK186 pKa = 10.35  FGIARR191 pKa = 11.84  PGNNLGLLDD200 pKa = 4.35  GFAGTLYY207 pKa = 10.06  CAKK210 pKa = 9.8  YY211 pKa = 8.17  VCKK214 pKa = 10.37  DD215 pKa = 3.27  ASVKK219 pKa = 10.12  NLEE222 pKa = 4.28  RR223 pKa = 11.84  KK224 pKa = 9.78  VYY226 pKa = 9.98  AQLSSILTDD235 pKa = 3.34  EE236 pKa = 4.64  NNRR239 pKa = 11.84  KK240 pKa = 9.32  PIEE243 pKa = 4.14  KK244 pKa = 9.99  RR245 pKa = 11.84  LTPSEE250 pKa = 4.18  LEE252 pKa = 3.75  KK253 pKa = 10.55  EE254 pKa = 4.03  IRR256 pKa = 11.84  SRR258 pKa = 11.84  LTDD261 pKa = 3.4  FRR263 pKa = 11.84  NRR265 pKa = 11.84  YY266 pKa = 6.18  SAKK269 pKa = 9.39  VRR271 pKa = 11.84  ISNGVGNYY279 pKa = 10.57  AFIHH283 pKa = 5.62  PSFRR287 pKa = 11.84  EE288 pKa = 4.05  DD289 pKa = 3.24  TFSVSMPHH297 pKa = 6.96  KK298 pKa = 10.68  NGFKK302 pKa = 10.1  RR303 pKa = 11.84  KK304 pKa = 9.63  KK305 pKa = 9.75  LGLYY309 pKa = 8.18  YY310 pKa = 10.2  FRR312 pKa = 11.84  KK313 pKa = 8.79  YY314 pKa = 9.59  FTDD317 pKa = 3.3  IVYY320 pKa = 10.55  DD321 pKa = 3.74  KK322 pKa = 10.45  NKK324 pKa = 10.1  SPRR327 pKa = 11.84  RR328 pKa = 11.84  VLNQRR333 pKa = 11.84  GIEE336 pKa = 3.9  YY337 pKa = 10.26  RR338 pKa = 11.84  IDD340 pKa = 3.38  HH341 pKa = 6.55  LGEE344 pKa = 4.15  GLEE347 pKa = 4.36  KK348 pKa = 10.43  KK349 pKa = 10.67  AKK351 pKa = 6.4  TTYY354 pKa = 10.56  KK355 pKa = 10.38  CVQFYY360 pKa = 10.86  LKK362 pKa = 10.64  NSEE365 pKa = 4.04  YY366 pKa = 9.98  RR367 pKa = 11.84  DD368 pKa = 3.54  KK369 pKa = 11.58  YY370 pKa = 9.24  MKK372 pKa = 10.42  SPHH375 pKa = 5.69  RR376 pKa = 11.84  TVEE379 pKa = 3.56  PKK381 pKa = 9.44  YY382 pKa = 8.97  TVKK385 pKa = 10.46  EE386 pKa = 4.01  LEE388 pKa = 4.16  RR389 pKa = 11.84  LLSIDD394 pKa = 3.71  NNDD397 pKa = 4.58  IINSMYY403 pKa = 10.53  YY404 pKa = 10.49  DD405 pKa = 3.32  YY406 pKa = 12.11  AMYY409 pKa = 10.79  KK410 pKa = 10.21  NIYY413 pKa = 7.04  EE414 pKa = 3.7  HH415 pKa = 6.96  HH416 pKa = 6.75  YY417 pKa = 7.96  YY418 pKa = 10.62  YY419 pKa = 10.57  EE420 pKa = 4.18  YY421 pKa = 10.7  TRR423 pKa = 11.84  EE424 pKa = 4.16  LDD426 pKa = 3.17  PMEE429 pKa = 5.04  DD430 pKa = 3.17  YY431 pKa = 11.17  RR432 pKa = 11.84  RR433 pKa = 11.84  FLTSTFGVDD442 pKa = 3.89  GLTTVDD448 pKa = 3.6  KK449 pKa = 10.06  TALPSGYY456 pKa = 9.54  YY457 pKa = 9.78  SYY459 pKa = 11.29  SRR461 pKa = 11.84  HH462 pKa = 5.96  PFFNEE467 pKa = 3.71  CTDD470 pKa = 3.96  LFSLLDD476 pKa = 4.16  DD477 pKa = 4.17  LVSFYY482 pKa = 10.8  FVEE485 pKa = 5.0  LSEE488 pKa = 4.41  KK489 pKa = 7.17  TQKK492 pKa = 10.83  DD493 pKa = 3.46  YY494 pKa = 11.8  EE495 pKa = 4.2  EE496 pKa = 4.3  RR497 pKa = 11.84  KK498 pKa = 8.32  RR499 pKa = 11.84  TKK501 pKa = 10.71  NMYY504 pKa = 10.08  RR505 pKa = 11.84  DD506 pKa = 3.31  LKK508 pKa = 10.62  LRR510 pKa = 11.84  KK511 pKa = 9.11  EE512 pKa = 4.2  GLFAVV517 pKa = 4.53  
 60.71 kDa
Isoelectric point according different methods: 
IPC2.protein.svr19  8.519 
IPC2_protein 8.595 
IPC_protein 8.463 
Toseland    8.858 
ProMoST     8.858 
Dawson      9.253 
Bjellqvist  9.107 
Wikipedia   9.487 
Rodwell     9.355 
Grimsley    9.355 
Solomon     9.297 
Lehninger   9.224 
Nozaki      9.033 
DTASelect   9.048 
Thurlkill   9.092 
EMBOSS      9.341 
Sillero     9.238 
Patrickios  3.999 
IPC_peptide 9.282 
IPC2_peptide  7.775 
IPC2.peptide.svr19  7.697 
 Peptides (in silico  digests for buttom-up proteomics) 
Below you can find 
in silico  digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
 
  
    Try  
 
  
    ChTry  
 
  
    ArgC  
 
  
    LysN  
 
  
    TryLysC  
   
  
General Statistics 
    
      
        Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
 
     
    
      
        4 
0
4 
1577
114
586
394.3
44.9
          
     
  
 
    Amino acid frequency 
  
  
    
      
        Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
 
     
    
                 
        6.531 ± 1.915
0.698 ± 0.283
6.468 ± 0.74
5.834 ± 1.023
5.263 ± 0.987
6.595 ± 0.678
1.458 ± 0.486
5.073 ± 0.396
7.229 ± 1.429
7.546 ± 0.553
          
     
  
  
  
      
          
        Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
 
     
    
                 
        2.029 ± 0.196
6.278 ± 0.805
3.044 ± 0.769
4.375 ± 1.445
4.629 ± 0.94
8.117 ± 1.114
6.785 ± 0.241
4.629 ± 0.331
0.888 ± 0.302
6.531 ± 1.46
          
     
  
  
  
Note:  For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein levelMost of the basic statistics you can see at this page can be downloaded from this CSV file  
For dipeptide frequency statistics click here