Pseudomonas phage phiB1_1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Autographiviridae; Krylovirinae; unclassified Krylovirinae

Average proteome isoelectric point is 6.61

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 52 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A7D7FQ11|A0A7D7FQ11_9CAUD HNHc domain-containing protein OS=Pseudomonas phage phiB1_1 OX=2755402 GN=phiB1_1_18 PE=4 SV=1
MM1 pKa = 7.44QEE3 pKa = 4.65FSPCQHH9 pKa = 6.98PLVDD13 pKa = 3.85VTSSGGLSQGLLDD26 pKa = 3.74YY27 pKa = 10.95SAAIDD32 pKa = 3.45QCNALNGLTPP42 pKa = 4.28

Molecular weight: 4.38 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A7D7JI42|A0A7D7JI42_9CAUD Exonuclease OS=Pseudomonas phage phiB1_1 OX=2755402 GN=phiB1_1_25 PE=4 SV=1
MM1 pKa = 7.37QPRR4 pKa = 11.84LEE6 pKa = 4.08QRR8 pKa = 11.84VSKK11 pKa = 10.67NLEE14 pKa = 3.33AFMRR18 pKa = 11.84GRR20 pKa = 11.84NKK22 pKa = 10.41DD23 pKa = 3.24RR24 pKa = 11.84SIKK27 pKa = 10.2SKK29 pKa = 10.61GVAAIEE35 pKa = 3.79DD36 pKa = 4.19TIARR40 pKa = 11.84FVNDD44 pKa = 3.58SSPEE48 pKa = 3.51ARR50 pKa = 11.84LAYY53 pKa = 9.87RR54 pKa = 11.84ASRR57 pKa = 11.84DD58 pKa = 3.74QIHH61 pKa = 6.75DD62 pKa = 3.69TDD64 pKa = 4.42PAPTSKK70 pKa = 10.8GAWFPKK76 pKa = 9.59KK77 pKa = 10.34SNRR80 pKa = 11.84QVRR83 pKa = 3.53

Molecular weight: 9.48 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

52

0

52

12903

42

1010

248.1

27.19

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

11.028 ± 0.428

1.24 ± 0.191

5.929 ± 0.226

5.224 ± 0.299

3.131 ± 0.18

7.983 ± 0.393

1.93 ± 0.231

4.115 ± 0.144

4.627 ± 0.225

8.936 ± 0.272

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.728 ± 0.137

3.945 ± 0.26

4.255 ± 0.3

4.867 ± 0.315

6.061 ± 0.284

6.161 ± 0.315

5.859 ± 0.335

7.425 ± 0.355

1.364 ± 0.126

3.193 ± 0.219
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski