Amino acid dipepetide frequency for Pseudomonas phage phiB1_1

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10-3.

For more information see sequence space article on Wikipedia.

AlaCysAspGluPheGlyHisIleLysLeuMetAsnProGlnArgSerThrValTrpTyrXaa
Ala
14.416AlaAla: 14.416 ± 1.667
1.163AlaCys: 1.163 ± 0.326
6.976AlaAsp: 6.976 ± 0.707
7.208AlaGlu: 7.208 ± 0.711
3.41AlaPhe: 3.41 ± 0.49
8.913AlaGly: 8.913 ± 1.245
1.473AlaHis: 1.473 ± 0.326
4.03AlaIle: 4.03 ± 0.644
5.658AlaLys: 5.658 ± 0.644
11.161AlaLeu: 11.161 ± 1.061
3.255AlaMet: 3.255 ± 0.444
4.65AlaAsn: 4.65 ± 0.725
3.255AlaPro: 3.255 ± 0.605
5.426AlaGln: 5.426 ± 0.623
6.278AlaArg: 6.278 ± 0.688
5.426AlaSer: 5.426 ± 0.703
6.511AlaThr: 6.511 ± 0.929
6.743AlaVal: 6.743 ± 0.547
1.395AlaTrp: 1.395 ± 0.32
2.945AlaTyr: 2.945 ± 0.521
0.0AlaXaa: 0.0 ± 0.0
Cys
0.853CysAla: 0.853 ± 0.25
0.233CysCys: 0.233 ± 0.138
0.775CysAsp: 0.775 ± 0.344
0.62CysGlu: 0.62 ± 0.285
0.465CysPhe: 0.465 ± 0.191
1.163CysGly: 1.163 ± 0.368
0.465CysHis: 0.465 ± 0.169
0.543CysIle: 0.543 ± 0.186
0.465CysLys: 0.465 ± 0.165
0.853CysLeu: 0.853 ± 0.197
0.543CysMet: 0.543 ± 0.194
0.62CysAsn: 0.62 ± 0.229
0.465CysPro: 0.465 ± 0.189
0.465CysGln: 0.465 ± 0.193
0.543CysArg: 0.543 ± 0.213
0.698CysSer: 0.698 ± 0.249
1.085CysThr: 1.085 ± 0.272
0.775CysVal: 0.775 ± 0.283
0.155CysTrp: 0.155 ± 0.093
0.62CysTyr: 0.62 ± 0.261
0.0CysXaa: 0.0 ± 0.0
Asp
5.891AspAla: 5.891 ± 0.637
0.775AspCys: 0.775 ± 0.243
2.868AspAsp: 2.868 ± 0.448
3.875AspGlu: 3.875 ± 0.566
1.783AspPhe: 1.783 ± 0.42
5.193AspGly: 5.193 ± 0.626
0.775AspHis: 0.775 ± 0.257
3.178AspIle: 3.178 ± 0.462
3.178AspLys: 3.178 ± 0.571
5.193AspLeu: 5.193 ± 0.682
1.86AspMet: 1.86 ± 0.394
1.86AspAsn: 1.86 ± 0.482
2.558AspPro: 2.558 ± 0.512
2.48AspGln: 2.48 ± 0.471
3.178AspArg: 3.178 ± 0.507
4.495AspSer: 4.495 ± 0.599
4.03AspThr: 4.03 ± 0.592
3.565AspVal: 3.565 ± 0.574
0.465AspTrp: 0.465 ± 0.18
2.093AspTyr: 2.093 ± 0.428
0.0AspXaa: 0.0 ± 0.0
Glu
6.433GluAla: 6.433 ± 0.689
0.698GluCys: 0.698 ± 0.302
3.333GluAsp: 3.333 ± 0.426
1.628GluGlu: 1.628 ± 0.405
2.635GluPhe: 2.635 ± 0.448
2.635GluGly: 2.635 ± 0.415
1.55GluHis: 1.55 ± 0.402
1.783GluIle: 1.783 ± 0.412
2.79GluLys: 2.79 ± 0.546
6.511GluLeu: 6.511 ± 0.897
1.24GluMet: 1.24 ± 0.328
1.395GluAsn: 1.395 ± 0.306
1.318GluPro: 1.318 ± 0.424
3.333GluGln: 3.333 ± 0.477
3.41GluArg: 3.41 ± 0.623
2.17GluSer: 2.17 ± 0.407
1.86GluThr: 1.86 ± 0.44
4.573GluVal: 4.573 ± 0.747
0.543GluTrp: 0.543 ± 0.189
2.403GluTyr: 2.403 ± 0.535
0.0GluXaa: 0.0 ± 0.0
Phe
1.938PheAla: 1.938 ± 0.294
0.62PheCys: 0.62 ± 0.225
2.48PheAsp: 2.48 ± 0.498
1.705PheGlu: 1.705 ± 0.472
1.008PhePhe: 1.008 ± 0.292
2.713PheGly: 2.713 ± 0.434
0.465PheHis: 0.465 ± 0.188
1.163PheIle: 1.163 ± 0.27
1.783PheLys: 1.783 ± 0.392
2.635PheLeu: 2.635 ± 0.537
0.853PheMet: 0.853 ± 0.289
1.628PheAsn: 1.628 ± 0.414
1.318PhePro: 1.318 ± 0.31
0.853PheGln: 0.853 ± 0.279
2.635PheArg: 2.635 ± 0.488
2.403PheSer: 2.403 ± 0.389
1.86PheThr: 1.86 ± 0.342
2.325PheVal: 2.325 ± 0.419
0.543PheTrp: 0.543 ± 0.241
0.388PheTyr: 0.388 ± 0.14
0.0PheXaa: 0.0 ± 0.0
Gly
7.363GlyAla: 7.363 ± 0.839
0.62GlyCys: 0.62 ± 0.222
5.348GlyAsp: 5.348 ± 0.508
3.798GlyGlu: 3.798 ± 0.489
2.713GlyPhe: 2.713 ± 0.534
6.821GlyGly: 6.821 ± 0.913
0.853GlyHis: 0.853 ± 0.252
4.185GlyIle: 4.185 ± 0.423
4.03GlyLys: 4.03 ± 0.618
6.201GlyLeu: 6.201 ± 0.765
2.248GlyMet: 2.248 ± 0.478
3.1GlyAsn: 3.1 ± 0.457
2.093GlyPro: 2.093 ± 0.385
4.108GlyGln: 4.108 ± 0.705
4.883GlyArg: 4.883 ± 0.524
4.573GlySer: 4.573 ± 0.682
5.581GlyThr: 5.581 ± 0.788
7.053GlyVal: 7.053 ± 0.807
1.473GlyTrp: 1.473 ± 0.366
2.79GlyTyr: 2.79 ± 0.45
0.0GlyXaa: 0.0 ± 0.0
His
1.86HisAla: 1.86 ± 0.408
0.465HisCys: 0.465 ± 0.196
1.395HisAsp: 1.395 ± 0.362
0.853HisGlu: 0.853 ± 0.267
0.93HisPhe: 0.93 ± 0.237
1.628HisGly: 1.628 ± 0.347
0.543HisHis: 0.543 ± 0.365
0.62HisIle: 0.62 ± 0.204
1.473HisLys: 1.473 ± 0.249
1.473HisLeu: 1.473 ± 0.389
0.543HisMet: 0.543 ± 0.228
0.62HisAsn: 0.62 ± 0.194
0.93HisPro: 0.93 ± 0.281
0.62HisGln: 0.62 ± 0.224
1.318HisArg: 1.318 ± 0.412
0.698HisSer: 0.698 ± 0.184
0.775HisThr: 0.775 ± 0.305
1.473HisVal: 1.473 ± 0.447
0.388HisTrp: 0.388 ± 0.196
0.698HisTyr: 0.698 ± 0.219
0.0HisXaa: 0.0 ± 0.0
Ile
4.185IleAla: 4.185 ± 0.596
0.388IleCys: 0.388 ± 0.204
2.868IleAsp: 2.868 ± 0.398
2.48IleGlu: 2.48 ± 0.383
0.93IlePhe: 0.93 ± 0.213
3.1IleGly: 3.1 ± 0.547
0.93IleHis: 0.93 ± 0.248
1.628IleIle: 1.628 ± 0.361
2.945IleLys: 2.945 ± 0.535
2.635IleLeu: 2.635 ± 0.412
0.93IleMet: 0.93 ± 0.257
2.17IleAsn: 2.17 ± 0.418
2.558IlePro: 2.558 ± 0.513
2.403IleGln: 2.403 ± 0.325
3.023IleArg: 3.023 ± 0.52
2.17IleSer: 2.17 ± 0.55
2.48IleThr: 2.48 ± 0.595
2.79IleVal: 2.79 ± 0.501
0.078IleTrp: 0.078 ± 0.08
0.465IleTyr: 0.465 ± 0.188
0.0IleXaa: 0.0 ± 0.0
Lys
5.581LysAla: 5.581 ± 0.851
0.853LysCys: 0.853 ± 0.301
2.403LysAsp: 2.403 ± 0.367
2.635LysGlu: 2.635 ± 0.491
1.628LysPhe: 1.628 ± 0.295
3.643LysGly: 3.643 ± 0.447
0.853LysHis: 0.853 ± 0.271
1.24LysIle: 1.24 ± 0.391
1.24LysLys: 1.24 ± 0.329
4.03LysLeu: 4.03 ± 0.498
0.543LysMet: 0.543 ± 0.188
1.55LysAsn: 1.55 ± 0.292
2.558LysPro: 2.558 ± 0.527
2.17LysGln: 2.17 ± 0.384
3.1LysArg: 3.1 ± 0.632
2.248LysSer: 2.248 ± 0.46
3.255LysThr: 3.255 ± 0.457
4.34LysVal: 4.34 ± 0.513
0.775LysTrp: 0.775 ± 0.214
1.628LysTyr: 1.628 ± 0.336
0.0LysXaa: 0.0 ± 0.0
Leu
9.998LeuAla: 9.998 ± 0.9
1.008LeuCys: 1.008 ± 0.281
5.891LeuAsp: 5.891 ± 0.779
4.418LeuGlu: 4.418 ± 0.71
2.015LeuPhe: 2.015 ± 0.387
7.286LeuGly: 7.286 ± 0.753
2.093LeuHis: 2.093 ± 0.468
3.178LeuIle: 3.178 ± 0.517
4.03LeuLys: 4.03 ± 0.633
6.898LeuLeu: 6.898 ± 0.857
2.79LeuMet: 2.79 ± 0.311
3.565LeuAsn: 3.565 ± 0.536
4.34LeuPro: 4.34 ± 0.893
4.418LeuGln: 4.418 ± 0.634
6.821LeuArg: 6.821 ± 0.787
5.891LeuSer: 5.891 ± 0.644
5.736LeuThr: 5.736 ± 0.581
5.813LeuVal: 5.813 ± 0.706
0.93LeuTrp: 0.93 ± 0.3
2.248LeuTyr: 2.248 ± 0.322
0.0LeuXaa: 0.0 ± 0.0
Met
3.643MetAla: 3.643 ± 0.652
0.155MetCys: 0.155 ± 0.109
1.473MetAsp: 1.473 ± 0.384
0.93MetGlu: 0.93 ± 0.248
1.085MetPhe: 1.085 ± 0.304
1.318MetGly: 1.318 ± 0.319
0.543MetHis: 0.543 ± 0.176
0.543MetIle: 0.543 ± 0.191
0.775MetLys: 0.775 ± 0.275
2.635MetLeu: 2.635 ± 0.471
0.698MetMet: 0.698 ± 0.213
0.93MetAsn: 0.93 ± 0.277
1.24MetPro: 1.24 ± 0.291
1.628MetGln: 1.628 ± 0.473
2.17MetArg: 2.17 ± 0.403
2.248MetSer: 2.248 ± 0.456
2.015MetThr: 2.015 ± 0.318
1.938MetVal: 1.938 ± 0.434
0.465MetTrp: 0.465 ± 0.216
0.853MetTyr: 0.853 ± 0.254
0.0MetXaa: 0.0 ± 0.0
Asn
5.271AsnAla: 5.271 ± 0.735
0.775AsnCys: 0.775 ± 0.341
2.325AsnAsp: 2.325 ± 0.478
1.938AsnGlu: 1.938 ± 0.413
1.163AsnPhe: 1.163 ± 0.348
3.41AsnGly: 3.41 ± 0.738
0.543AsnHis: 0.543 ± 0.229
2.015AsnIle: 2.015 ± 0.358
2.325AsnLys: 2.325 ± 0.483
3.41AsnLeu: 3.41 ± 0.396
0.62AsnMet: 0.62 ± 0.167
1.24AsnAsn: 1.24 ± 0.332
2.558AsnPro: 2.558 ± 0.324
1.473AsnGln: 1.473 ± 0.289
1.163AsnArg: 1.163 ± 0.289
2.015AsnSer: 2.015 ± 0.371
2.17AsnThr: 2.17 ± 0.51
3.178AsnVal: 3.178 ± 0.459
0.62AsnTrp: 0.62 ± 0.194
1.24AsnTyr: 1.24 ± 0.311
0.0AsnXaa: 0.0 ± 0.0
Pro
5.813ProAla: 5.813 ± 0.809
0.62ProCys: 0.62 ± 0.283
2.325ProAsp: 2.325 ± 0.421
3.488ProGlu: 3.488 ± 0.575
0.93ProPhe: 0.93 ± 0.222
3.178ProGly: 3.178 ± 0.449
0.775ProHis: 0.775 ± 0.228
1.628ProIle: 1.628 ± 0.412
1.86ProLys: 1.86 ± 0.446
3.1ProLeu: 3.1 ± 0.616
0.775ProMet: 0.775 ± 0.229
1.628ProAsn: 1.628 ± 0.392
1.395ProPro: 1.395 ± 0.373
1.473ProGln: 1.473 ± 0.3
2.17ProArg: 2.17 ± 0.403
2.79ProSer: 2.79 ± 0.423
2.248ProThr: 2.248 ± 0.596
3.953ProVal: 3.953 ± 0.688
0.775ProTrp: 0.775 ± 0.218
1.628ProTyr: 1.628 ± 0.365
0.0ProXaa: 0.0 ± 0.0
Gln
6.433GlnAla: 6.433 ± 0.787
0.31GlnCys: 0.31 ± 0.167
1.705GlnAsp: 1.705 ± 0.334
2.713GlnGlu: 2.713 ± 0.639
1.395GlnPhe: 1.395 ± 0.254
3.875GlnGly: 3.875 ± 0.529
1.705GlnHis: 1.705 ± 0.356
1.628GlnIle: 1.628 ± 0.413
1.318GlnLys: 1.318 ± 0.32
6.123GlnLeu: 6.123 ± 0.703
1.24GlnMet: 1.24 ± 0.372
2.17GlnAsn: 2.17 ± 0.439
1.395GlnPro: 1.395 ± 0.354
3.023GlnGln: 3.023 ± 0.468
2.945GlnArg: 2.945 ± 0.434
2.945GlnSer: 2.945 ± 0.431
2.17GlnThr: 2.17 ± 0.464
3.023GlnVal: 3.023 ± 0.505
0.62GlnTrp: 0.62 ± 0.225
1.938GlnTyr: 1.938 ± 0.352
0.0GlnXaa: 0.0 ± 0.0
Arg
5.891ArgAla: 5.891 ± 0.527
0.93ArgCys: 0.93 ± 0.28
3.565ArgAsp: 3.565 ± 0.478
3.953ArgGlu: 3.953 ± 0.587
2.248ArgPhe: 2.248 ± 0.525
4.495ArgGly: 4.495 ± 0.646
1.395ArgHis: 1.395 ± 0.374
3.798ArgIle: 3.798 ± 0.559
3.178ArgLys: 3.178 ± 0.597
4.805ArgLeu: 4.805 ± 0.597
1.938ArgMet: 1.938 ± 0.521
2.558ArgAsn: 2.558 ± 0.455
1.628ArgPro: 1.628 ± 0.363
2.79ArgGln: 2.79 ± 0.557
4.418ArgArg: 4.418 ± 0.59
3.333ArgSer: 3.333 ± 0.56
2.17ArgThr: 2.17 ± 0.463
4.65ArgVal: 4.65 ± 0.465
0.853ArgTrp: 0.853 ± 0.253
2.015ArgTyr: 2.015 ± 0.485
0.0ArgXaa: 0.0 ± 0.0
Ser
6.898SerAla: 6.898 ± 0.684
0.698SerCys: 0.698 ± 0.226
3.798SerAsp: 3.798 ± 0.502
2.868SerGlu: 2.868 ± 0.367
2.48SerPhe: 2.48 ± 0.413
4.96SerGly: 4.96 ± 0.522
1.008SerHis: 1.008 ± 0.239
3.1SerIle: 3.1 ± 0.529
3.178SerLys: 3.178 ± 0.562
4.805SerLeu: 4.805 ± 0.667
1.783SerMet: 1.783 ± 0.349
2.48SerAsn: 2.48 ± 0.654
2.015SerPro: 2.015 ± 0.439
2.403SerGln: 2.403 ± 0.564
3.255SerArg: 3.255 ± 0.538
4.418SerSer: 4.418 ± 0.803
3.72SerThr: 3.72 ± 0.594
4.573SerVal: 4.573 ± 0.944
1.085SerTrp: 1.085 ± 0.308
2.093SerTyr: 2.093 ± 0.53
0.0SerXaa: 0.0 ± 0.0
Thr
6.356ThrAla: 6.356 ± 0.886
0.62ThrCys: 0.62 ± 0.227
3.023ThrAsp: 3.023 ± 0.549
1.783ThrGlu: 1.783 ± 0.433
1.473ThrPhe: 1.473 ± 0.317
7.363ThrGly: 7.363 ± 0.939
0.62ThrHis: 0.62 ± 0.228
2.558ThrIle: 2.558 ± 0.464
1.705ThrLys: 1.705 ± 0.329
5.658ThrLeu: 5.658 ± 0.524
2.093ThrMet: 2.093 ± 0.309
2.015ThrAsn: 2.015 ± 0.404
4.03ThrPro: 4.03 ± 0.701
3.41ThrGln: 3.41 ± 0.511
2.635ThrArg: 2.635 ± 0.513
3.41ThrSer: 3.41 ± 0.584
3.333ThrThr: 3.333 ± 0.558
4.263ThrVal: 4.263 ± 0.765
0.465ThrTrp: 0.465 ± 0.161
1.783ThrTyr: 1.783 ± 0.462
0.0ThrXaa: 0.0 ± 0.0
Val
6.898ValAla: 6.898 ± 0.795
0.62ValCys: 0.62 ± 0.213
5.115ValAsp: 5.115 ± 0.688
3.41ValGlu: 3.41 ± 0.557
1.473ValPhe: 1.473 ± 0.412
5.193ValGly: 5.193 ± 0.626
1.86ValHis: 1.86 ± 0.442
2.713ValIle: 2.713 ± 0.569
2.635ValLys: 2.635 ± 0.548
6.821ValLeu: 6.821 ± 0.886
2.325ValMet: 2.325 ± 0.45
2.713ValAsn: 2.713 ± 0.403
4.573ValPro: 4.573 ± 0.767
3.953ValGln: 3.953 ± 0.625
3.72ValArg: 3.72 ± 0.45
6.201ValSer: 6.201 ± 1.033
4.805ValThr: 4.805 ± 0.84
5.348ValVal: 5.348 ± 0.8
1.395ValTrp: 1.395 ± 0.352
2.403ValTyr: 2.403 ± 0.376
0.0ValXaa: 0.0 ± 0.0
Trp
1.628TrpAla: 1.628 ± 0.413
0.543TrpCys: 0.543 ± 0.19
0.543TrpAsp: 0.543 ± 0.27
0.62TrpGlu: 0.62 ± 0.218
0.698TrpPhe: 0.698 ± 0.233
0.62TrpGly: 0.62 ± 0.207
0.31TrpHis: 0.31 ± 0.144
0.543TrpIle: 0.543 ± 0.245
0.543TrpLys: 0.543 ± 0.211
1.318TrpLeu: 1.318 ± 0.381
0.388TrpMet: 0.388 ± 0.184
0.62TrpAsn: 0.62 ± 0.226
0.62TrpPro: 0.62 ± 0.19
0.543TrpGln: 0.543 ± 0.205
0.62TrpArg: 0.62 ± 0.232
1.163TrpSer: 1.163 ± 0.343
0.853TrpThr: 0.853 ± 0.25
0.698TrpVal: 0.698 ± 0.263
0.155TrpTrp: 0.155 ± 0.117
0.62TrpTyr: 0.62 ± 0.22
0.0TrpXaa: 0.0 ± 0.0
Tyr
2.945TyrAla: 2.945 ± 0.454
0.31TyrCys: 0.31 ± 0.191
1.085TyrAsp: 1.085 ± 0.331
1.318TyrGlu: 1.318 ± 0.377
0.853TyrPhe: 0.853 ± 0.209
2.325TyrGly: 2.325 ± 0.4
0.543TyrHis: 0.543 ± 0.161
1.085TyrIle: 1.085 ± 0.282
1.163TyrLys: 1.163 ± 0.273
3.1TyrLeu: 3.1 ± 0.462
0.543TyrMet: 0.543 ± 0.171
1.938TyrAsn: 1.938 ± 0.359
1.705TyrPro: 1.705 ± 0.33
1.705TyrGln: 1.705 ± 0.333
2.325TyrArg: 2.325 ± 0.37
2.325TyrSer: 2.325 ± 0.408
1.938TyrThr: 1.938 ± 0.38
3.178TyrVal: 3.178 ± 0.549
0.465TyrTrp: 0.465 ± 0.179
1.085TyrTyr: 1.085 ± 0.379
0.0TyrXaa: 0.0 ± 0.0
Xaa
0.0XaaAla: 0.0 ± 0.0
0.0XaaCys: 0.0 ± 0.0
0.0XaaAsp: 0.0 ± 0.0
0.0XaaGlu: 0.0 ± 0.0
0.0XaaPhe: 0.0 ± 0.0
0.0XaaGly: 0.0 ± 0.0
0.0XaaHis: 0.0 ± 0.0
0.0XaaIle: 0.0 ± 0.0
0.0XaaLys: 0.0 ± 0.0
0.0XaaLeu: 0.0 ± 0.0
0.0XaaMet: 0.0 ± 0.0
0.0XaaAsn: 0.0 ± 0.0
0.0XaaPro: 0.0 ± 0.0
0.0XaaGln: 0.0 ± 0.0
0.0XaaArg: 0.0 ± 0.0
0.0XaaSer: 0.0 ± 0.0
0.0XaaThr: 0.0 ± 0.0
0.0XaaVal: 0.0 ± 0.0
0.0XaaTrp: 0.0 ± 0.0
0.0XaaTyr: 0.0 ± 0.0
0.0XaaXaa: 0.0 ± 0.0
Statistics based on 52 proteins (12903 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski