Finegoldia magna (strain ATCC 29328 / DSM 20472 / WAL 2508) (Peptostreptococcus magnus)
Average proteome isoelectric point is 6.43
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 1813 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|B0S4D2|B0S4D2_FINM2 dUTP diphosphatase OS=Finegoldia magna (strain ATCC 29328 / DSM 20472 / WAL 2508) OX=334413 GN=FMG_P0074 PE=4 SV=1
MM1 pKa = 7.22 TNEE4 pKa = 4.29 LKK6 pKa = 10.35 PYY8 pKa = 10.21 EE9 pKa = 4.49 KK10 pKa = 9.84 IKK12 pKa = 10.64 EE13 pKa = 3.99 YY14 pKa = 10.11 TVNGKK19 pKa = 9.38 PYY21 pKa = 10.67 DD22 pKa = 3.78 EE23 pKa = 4.81 GLEE26 pKa = 3.99 DD27 pKa = 5.13 LYY29 pKa = 11.55 LYY31 pKa = 10.55 LQATASIDD39 pKa = 3.22 YY40 pKa = 8.83 TIYY43 pKa = 11.18 VDD45 pKa = 5.39 IEE47 pKa = 4.28 TGDD50 pKa = 3.51 IDD52 pKa = 5.79 AMPSCDD58 pKa = 5.32 FGNAYY63 pKa = 10.93 YY64 pKa = 10.52 NINKK68 pKa = 10.22 AIFNWKK74 pKa = 9.54 VNGGEE79 pKa = 3.82 PWLGEE84 pKa = 4.01 VYY86 pKa = 10.06 TYY88 pKa = 11.43 DD89 pKa = 3.48 EE90 pKa = 5.07 LEE92 pKa = 4.87 DD93 pKa = 3.91 YY94 pKa = 11.12 ACEE97 pKa = 3.97 EE98 pKa = 4.21 DD99 pKa = 3.91 VKK101 pKa = 10.59 EE102 pKa = 3.87 LRR104 pKa = 11.84 KK105 pKa = 10.18 EE106 pKa = 3.86 FEE108 pKa = 4.23 EE109 pKa = 4.13 EE110 pKa = 3.89 HH111 pKa = 7.59 KK112 pKa = 11.17 EE113 pKa = 3.98 EE114 pKa = 4.4 LDD116 pKa = 3.57 EE117 pKa = 5.04 YY118 pKa = 11.37 EE119 pKa = 5.63 DD120 pKa = 5.52 IEE122 pKa = 5.58 DD123 pKa = 4.92 FYY125 pKa = 11.87 NDD127 pKa = 3.16 FANDD131 pKa = 3.31 YY132 pKa = 11.14 KK133 pKa = 11.23 EE134 pKa = 3.95 FLFYY138 pKa = 11.27 AEE140 pKa = 5.4 NEE142 pKa = 4.59 DD143 pKa = 3.14 IDD145 pKa = 4.28 KK146 pKa = 11.37 GVDD149 pKa = 2.98 EE150 pKa = 5.21 FARR153 pKa = 11.84 EE154 pKa = 4.2 SLDD157 pKa = 3.1 SWKK160 pKa = 10.81 EE161 pKa = 3.85 DD162 pKa = 4.21 FEE164 pKa = 4.43 NAYY167 pKa = 10.6 CKK169 pKa = 10.59 LLEE172 pKa = 4.71 DD173 pKa = 3.88 YY174 pKa = 10.37 EE175 pKa = 4.29 LRR177 pKa = 11.84 EE178 pKa = 4.04 EE179 pKa = 4.59 LEE181 pKa = 4.8 DD182 pKa = 3.84 YY183 pKa = 11.21
Molecular weight: 21.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.785
IPC2_protein 3.834
IPC_protein 3.808
Toseland 3.617
ProMoST 3.935
Dawson 3.77
Bjellqvist 3.923
Wikipedia 3.656
Rodwell 3.643
Grimsley 3.528
Solomon 3.77
Lehninger 3.719
Nozaki 3.884
DTASelect 4.037
Thurlkill 3.643
EMBOSS 3.668
Sillero 3.923
Patrickios 0.553
IPC_peptide 3.77
IPC2_peptide 3.91
IPC2.peptide.svr19 3.837
Protein with the highest isoelectric point:
>tr|B0S3X3|B0S3X3_FINM2 Uncharacterized protein OS=Finegoldia magna (strain ATCC 29328 / DSM 20472 / WAL 2508) OX=334413 GN=FMG_0329 PE=4 SV=1
MM1 pKa = 7.75 RR2 pKa = 11.84 LMKK5 pKa = 9.87 RR6 pKa = 11.84 TYY8 pKa = 10.02 QPNNRR13 pKa = 11.84 KK14 pKa = 9.34 RR15 pKa = 11.84 KK16 pKa = 8.55 KK17 pKa = 10.22 DD18 pKa = 3.02 HH19 pKa = 6.06 GFRR22 pKa = 11.84 NRR24 pKa = 11.84 MATRR28 pKa = 11.84 GGRR31 pKa = 11.84 AVLKK35 pKa = 10.23 ARR37 pKa = 11.84 RR38 pKa = 11.84 RR39 pKa = 11.84 KK40 pKa = 8.76 GRR42 pKa = 11.84 KK43 pKa = 8.75 VLSAA47 pKa = 4.05
Molecular weight: 5.65 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.356
IPC2_protein 10.906
IPC_protein 12.325
Toseland 12.486
ProMoST 12.983
Dawson 12.486
Bjellqvist 12.486
Wikipedia 12.969
Rodwell 12.223
Grimsley 12.53
Solomon 12.983
Lehninger 12.881
Nozaki 12.486
DTASelect 12.486
Thurlkill 12.486
EMBOSS 12.983
Sillero 12.486
Patrickios 11.959
IPC_peptide 12.983
IPC2_peptide 11.974
IPC2.peptide.svr19 9.069
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
1813
0
1813
596766
16
4919
329.2
37.43
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.099 ± 0.059
1.009 ± 0.025
6.662 ± 0.068
7.403 ± 0.075
4.627 ± 0.057
5.702 ± 0.053
1.33 ± 0.018
9.231 ± 0.078
9.772 ± 0.079
8.841 ± 0.079
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.653 ± 0.034
6.486 ± 0.053
2.665 ± 0.039
2.633 ± 0.032
3.372 ± 0.039
6.272 ± 0.051
5.027 ± 0.066
6.501 ± 0.043
0.548 ± 0.016
4.167 ± 0.043
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here