Plasmodium cynomolgi (strain B)
Average proteome isoelectric point is 7.01
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5713 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|K6UJA2|K6UJA2_PLACD HIT-type domain-containing protein OS=Plasmodium cynomolgi (strain B) OX=1120755 GN=PCYB_072450 PE=4 SV=1
MM1 pKa = 7.54 KK2 pKa = 10.55 NSLCLIFCLFFLNEE16 pKa = 4.18 FACGEE21 pKa = 4.06 RR22 pKa = 11.84 EE23 pKa = 4.01 EE24 pKa = 5.11 TIDD27 pKa = 3.72 AVPNVAVVNLEE38 pKa = 3.89 KK39 pKa = 9.69 PTLRR43 pKa = 11.84 TLLMEE48 pKa = 4.4 NPGVVGEE55 pKa = 4.25 EE56 pKa = 4.11 VSEE59 pKa = 4.52 GVANGQVVNEE69 pKa = 4.55 GVPSDD74 pKa = 4.64 DD75 pKa = 4.49 LPVDD79 pKa = 4.2 RR80 pKa = 11.84 VATEE84 pKa = 3.95 EE85 pKa = 4.09 VSPEE89 pKa = 3.87 VAPSDD94 pKa = 4.12 DD95 pKa = 3.98 APSALSVEE103 pKa = 4.46 TAEE106 pKa = 5.13 GEE108 pKa = 4.3 NQEE111 pKa = 4.99 SEE113 pKa = 4.5 QKK115 pKa = 9.51 TPPDD119 pKa = 3.3 NAKK122 pKa = 10.34 QSDD125 pKa = 3.96 ATPVEE130 pKa = 4.47 VTHH133 pKa = 6.33 GVNSVVEE140 pKa = 4.15 NEE142 pKa = 4.22 EE143 pKa = 4.12 GKK145 pKa = 9.97 HH146 pKa = 5.15 VEE148 pKa = 3.9 NDD150 pKa = 3.55 SPVVTKK156 pKa = 10.4 GQQEE160 pKa = 4.18 EE161 pKa = 4.92 GEE163 pKa = 4.46 EE164 pKa = 4.35 CCNEE168 pKa = 3.92 CSTPCVPEE176 pKa = 4.06 CTEE179 pKa = 4.29 GDD181 pKa = 3.67 NNCPEE186 pKa = 4.22 KK187 pKa = 10.94 CSTDD191 pKa = 3.58 DD192 pKa = 4.62 ACTEE196 pKa = 4.04 DD197 pKa = 4.63 CVVHH201 pKa = 6.02 EE202 pKa = 4.4 NCEE205 pKa = 4.09 VADD208 pKa = 4.08 CPEE211 pKa = 4.39 CSGCPKK217 pKa = 10.65 DD218 pKa = 4.24 SACSEE223 pKa = 4.49 CPTDD227 pKa = 5.19 SEE229 pKa = 5.37 CPQCSKK235 pKa = 10.84 CPQCAEE241 pKa = 4.38 CPQCSKK247 pKa = 10.93 CPQCAEE253 pKa = 3.97 NPQCSKK259 pKa = 10.85 CPQCAEE265 pKa = 4.11 CPEE268 pKa = 4.39 CSKK271 pKa = 11.12 CPEE274 pKa = 4.07 CSKK277 pKa = 10.75 CLEE280 pKa = 4.87 DD281 pKa = 4.2 STCDD285 pKa = 3.27 EE286 pKa = 4.58 CAKK289 pKa = 10.63 CPEE292 pKa = 4.46 DD293 pKa = 4.74 PEE295 pKa = 4.37 CAEE298 pKa = 4.64 CKK300 pKa = 10.07 RR301 pKa = 11.84 EE302 pKa = 4.0 CEE304 pKa = 3.95 MRR306 pKa = 11.84 TEE308 pKa = 3.97 EE309 pKa = 5.32 GEE311 pKa = 4.1 NQVAEE316 pKa = 4.32 EE317 pKa = 4.32 RR318 pKa = 11.84 TSEE321 pKa = 4.61 DD322 pKa = 2.93 SPQTVDD328 pKa = 3.26 TPHH331 pKa = 7.52 VEE333 pKa = 4.38 VVNSVDD339 pKa = 3.53 PVAEE343 pKa = 4.04 EE344 pKa = 4.73 AEE346 pKa = 4.56 GKK348 pKa = 9.89 QPEE351 pKa = 4.45 IEE353 pKa = 4.56 DD354 pKa = 3.65 NSDD357 pKa = 3.41 GEE359 pKa = 4.42 GANNVIRR366 pKa = 11.84 EE367 pKa = 4.19 IFNGLKK373 pKa = 10.22 NVEE376 pKa = 4.37 DD377 pKa = 4.0 EE378 pKa = 4.53 EE379 pKa = 4.51 EE380 pKa = 4.41 TVLEE384 pKa = 4.23 EE385 pKa = 4.03 TDD387 pKa = 3.78 EE388 pKa = 4.45 EE389 pKa = 4.67 EE390 pKa = 4.48 GDD392 pKa = 3.74 QMVDD396 pKa = 3.11 EE397 pKa = 4.76 NEE399 pKa = 4.06 EE400 pKa = 4.07 EE401 pKa = 4.55 EE402 pKa = 4.35 EE403 pKa = 4.66 GEE405 pKa = 4.38 TYY407 pKa = 10.45 EE408 pKa = 5.83 GEE410 pKa = 4.22 DD411 pKa = 3.91 TEE413 pKa = 6.03 DD414 pKa = 3.36 VALDD418 pKa = 3.71 EE419 pKa = 4.62 EE420 pKa = 4.79 DD421 pKa = 3.93 EE422 pKa = 4.31 EE423 pKa = 5.13 GEE425 pKa = 3.95 KK426 pKa = 11.03 DD427 pKa = 3.64 EE428 pKa = 4.51 EE429 pKa = 4.54 GKK431 pKa = 10.72 AGEE434 pKa = 4.44 EE435 pKa = 4.45 GNAGEE440 pKa = 4.34 GGKK443 pKa = 10.39 AGEE446 pKa = 4.08 EE447 pKa = 4.29 GKK449 pKa = 10.64 AGEE452 pKa = 4.15 EE453 pKa = 4.37 GKK455 pKa = 10.58 AGEE458 pKa = 4.4 QSVEE462 pKa = 4.04 EE463 pKa = 4.2 QNAEE467 pKa = 3.96 VPFIDD472 pKa = 3.86 TATNNKK478 pKa = 8.54 GVRR481 pKa = 11.84 NIKK484 pKa = 9.36 TEE486 pKa = 3.92 THH488 pKa = 6.23 NLVADD493 pKa = 5.81 LITILNADD501 pKa = 3.62 NGIDD505 pKa = 3.61 QSLKK509 pKa = 11.0 ALAKK513 pKa = 10.85 DD514 pKa = 3.2 MAQYY518 pKa = 10.62 FLNHH522 pKa = 6.31 LNAEE526 pKa = 4.14 NEE528 pKa = 4.15 IAA530 pKa = 4.82
Molecular weight: 57.15 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.775
IPC2_protein 3.872
IPC_protein 3.834
Toseland 3.668
ProMoST 3.935
Dawson 3.783
Bjellqvist 3.935
Wikipedia 3.63
Rodwell 3.668
Grimsley 3.579
Solomon 3.77
Lehninger 3.732
Nozaki 3.884
DTASelect 3.999
Thurlkill 3.668
EMBOSS 3.643
Sillero 3.948
Patrickios 0.769
IPC_peptide 3.783
IPC2_peptide 3.935
IPC2.peptide.svr19 3.844
Protein with the highest isoelectric point:
>tr|K6UWW3|K6UWW3_PLACD DNA helicase OS=Plasmodium cynomolgi (strain B) OX=1120755 GN=PCYB_102750 PE=4 SV=1
MM1 pKa = 7.28 AHH3 pKa = 6.31 GASRR7 pKa = 11.84 YY8 pKa = 8.75 KK9 pKa = 10.48 KK10 pKa = 10.3 SRR12 pKa = 11.84 AKK14 pKa = 9.88 MRR16 pKa = 11.84 WKK18 pKa = 9.16 WKK20 pKa = 9.29 KK21 pKa = 9.72 KK22 pKa = 6.67 RR23 pKa = 11.84 TRR25 pKa = 11.84 RR26 pKa = 11.84 LQKK29 pKa = 9.69 KK30 pKa = 7.67 RR31 pKa = 11.84 RR32 pKa = 11.84 KK33 pKa = 8.24 MRR35 pKa = 11.84 QRR37 pKa = 11.84 SRR39 pKa = 3.22
Molecular weight: 5.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.436
IPC2_protein 11.14
IPC_protein 12.501
Toseland 12.676
ProMoST 13.159
Dawson 12.676
Bjellqvist 12.661
Wikipedia 13.144
Rodwell 12.486
Grimsley 12.705
Solomon 13.159
Lehninger 13.056
Nozaki 12.661
DTASelect 12.661
Thurlkill 12.661
EMBOSS 13.159
Sillero 12.661
Patrickios 12.193
IPC_peptide 13.159
IPC2_peptide 12.149
IPC2.peptide.svr19 9.032
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5713
0
5713
3284714
16
8883
575.0
65.71
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
4.437 ± 0.029
1.892 ± 0.016
5.74 ± 0.026
7.705 ± 0.048
4.28 ± 0.03
5.966 ± 0.051
2.639 ± 0.016
6.229 ± 0.04
9.364 ± 0.039
8.129 ± 0.041
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.158 ± 0.011
8.02 ± 0.046
3.117 ± 0.025
3.198 ± 0.016
4.561 ± 0.034
7.982 ± 0.035
4.497 ± 0.018
5.161 ± 0.02
0.632 ± 0.008
4.287 ± 0.026
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here