Proteus mirabilis (strain HI4320)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Gammaproteobacteria; Enterobacterales; Morganellaceae; Proteus; Proteus mirabilis

Average proteome isoelectric point is 6.56

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3661 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|B4F0P0|B4F0P0_PROMH Putative monooxygenase OS=Proteus mirabilis (strain HI4320) OX=529507 GN=visC PE=3 SV=1
MM1 pKa = 8.15RR2 pKa = 11.84IYY4 pKa = 9.21TQQGDD9 pKa = 4.21TVDD12 pKa = 4.7DD13 pKa = 3.61ICWRR17 pKa = 11.84YY18 pKa = 9.45FGQSSGMIEE27 pKa = 4.06QVLEE31 pKa = 4.2ANPGLVEE38 pKa = 5.0LGAILPTGTAIEE50 pKa = 4.83LPDD53 pKa = 3.86TPQQHH58 pKa = 5.6STTPILQLWDD68 pKa = 3.3

Molecular weight: 7.56 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|B4F0U5|DNAA_PROMH Chromosomal replication initiator protein DnaA OS=Proteus mirabilis (strain HI4320) OX=529507 GN=dnaA PE=3 SV=1
MM1 pKa = 7.45KK2 pKa = 9.51RR3 pKa = 11.84TFQPSVLKK11 pKa = 10.65RR12 pKa = 11.84NRR14 pKa = 11.84NHH16 pKa = 6.8GFRR19 pKa = 11.84ARR21 pKa = 11.84MATKK25 pKa = 10.4NGRR28 pKa = 11.84QVLARR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AKK37 pKa = 10.22GRR39 pKa = 11.84ARR41 pKa = 11.84LTVSSKK47 pKa = 11.16

Molecular weight: 5.49 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3661

0

3661

1149705

30

4620

314.0

35.08

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

8.148 ± 0.051

1.068 ± 0.017

5.163 ± 0.029

5.879 ± 0.047

4.095 ± 0.03

6.647 ± 0.046

2.207 ± 0.022

7.298 ± 0.038

5.596 ± 0.036

10.418 ± 0.047

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.583 ± 0.023

4.706 ± 0.045

4.057 ± 0.028

4.687 ± 0.041

4.683 ± 0.033

6.361 ± 0.037

5.499 ± 0.033

6.301 ± 0.04

1.276 ± 0.017

3.327 ± 0.029
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski