Erythrobacter sp. HI00D59

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Sphingomonadales; Erythrobacteraceae; Erythrobacter/Porphyrobacter group; Erythrobacter; unclassified Erythrobacter

Average proteome isoelectric point is 5.94

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 3091 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|A0A165QJX8|A0A165QJX8_9SPHN Transcription termination/antitermination protein NusG OS=Erythrobacter sp. HI00D59 OX=1822215 GN=nusG PE=3 SV=1
MM1 pKa = 7.58KK2 pKa = 10.46SLFAAATGTLALACAAPAMAQNVEE26 pKa = 4.23ALPEE30 pKa = 4.29GAEE33 pKa = 4.03DD34 pKa = 4.47TVFDD38 pKa = 4.29DD39 pKa = 3.39TWVSVGVGAIYY50 pKa = 10.46SPSYY54 pKa = 10.55DD55 pKa = 3.29GSDD58 pKa = 3.84DD59 pKa = 3.95YY60 pKa = 11.75VVSAIPIIQGKK71 pKa = 9.04VGGVRR76 pKa = 11.84ISPRR80 pKa = 11.84PGGAALDD87 pKa = 4.19FVDD90 pKa = 5.66DD91 pKa = 4.36DD92 pKa = 4.68SEE94 pKa = 4.33GARR97 pKa = 11.84ISAGVAAKK105 pKa = 10.51LNRR108 pKa = 11.84NRR110 pKa = 11.84ASQVEE115 pKa = 4.29DD116 pKa = 3.78EE117 pKa = 4.75VVLSYY122 pKa = 11.86GEE124 pKa = 4.83LDD126 pKa = 3.46TAFEE130 pKa = 4.49VGPSIGVSFPGVLNPYY146 pKa = 10.36DD147 pKa = 3.74SVSFNIDD154 pKa = 3.5TLWDD158 pKa = 3.25VAGAHH163 pKa = 7.26DD164 pKa = 4.93GMTVNPGVTYY174 pKa = 7.06FTPVSRR180 pKa = 11.84GAAISLSVSGTYY192 pKa = 10.41VDD194 pKa = 5.76DD195 pKa = 5.96DD196 pKa = 4.05YY197 pKa = 12.24ADD199 pKa = 3.75YY200 pKa = 10.75YY201 pKa = 11.34YY202 pKa = 10.97SVPAGGALPAFQADD216 pKa = 4.07GGWDD220 pKa = 3.37SVGANLLVGVDD231 pKa = 4.21LDD233 pKa = 4.23GNLANGGLSLIALGGYY249 pKa = 8.72SRR251 pKa = 11.84QLGDD255 pKa = 3.75SKK257 pKa = 10.55NTPFTAIRR265 pKa = 11.84GDD267 pKa = 3.28ADD269 pKa = 2.86QWMGAIGIGYY279 pKa = 6.86TFF281 pKa = 4.31

Molecular weight: 28.88 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|A0A165RUC0|A0A165RUC0_9SPHN Uncharacterized protein OS=Erythrobacter sp. HI00D59 OX=1822215 GN=A3711_12195 PE=4 SV=1
MM1 pKa = 7.45KK2 pKa = 9.61RR3 pKa = 11.84TFQPSNLVRR12 pKa = 11.84ARR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.89GFFARR21 pKa = 11.84KK22 pKa = 7.42ATPGGRR28 pKa = 11.84KK29 pKa = 7.9VLRR32 pKa = 11.84ARR34 pKa = 11.84RR35 pKa = 11.84SRR37 pKa = 11.84GRR39 pKa = 11.84KK40 pKa = 8.41NLCAA44 pKa = 4.54

Molecular weight: 5.11 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

3091

0

3091

982353

41

2006

317.8

34.49

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

12.753 ± 0.061

0.815 ± 0.015

6.287 ± 0.035

6.465 ± 0.042

3.657 ± 0.028

8.863 ± 0.049

1.971 ± 0.024

4.955 ± 0.029

2.965 ± 0.037

9.834 ± 0.052

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.473 ± 0.022

2.514 ± 0.027

5.042 ± 0.028

3.155 ± 0.025

7.183 ± 0.043

5.206 ± 0.029

5.131 ± 0.033

7.068 ± 0.033

1.426 ± 0.019

2.234 ± 0.022
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski