Amino acid dipepetide frequency for Erythrobacter sp. HI00D59

Apart from single amino acid frequencies one can also calculate so called amino acid dipeptide frequency.

There is 400 possibilites (441 if we consider Xaa as additional 21st amino acid representing all non-standard or unknown amino acids). Thus, if the dipeptides would be present randomly in proteins each amino acid dipeptide should be present with 0.25% frequency. As it is not the case in the nature, for better visablity all more than expected dipepetides are marked by red, and those which are underrepresented are marked by blue in the table.

All values are presented as per milles (‰), therefore need to be multiplied by 10^-3.

For more information see sequence space article on Wikipedia.

	Ala	Cys	Asp	Glu	Phe	Gly	His	Ile	Lys	Leu	Met	Asn	Pro	Gln	Arg	Ser	Thr	Val	Trp	Tyr	Xaa
Ala	16.923AlaAla: 16.923 ± 0.187	1.113AlaCys: 1.113 ± 0.035	7.399AlaAsp: 7.399 ± 0.09	8.501AlaGlu: 8.501 ± 0.109	4.302AlaPhe: 4.302 ± 0.068	10.833AlaGly: 10.833 ± 0.117	2.312AlaHis: 2.312 ± 0.05	6.805AlaIle: 6.805 ± 0.088	4.049AlaLys: 4.049 ± 0.078	13.595AlaLeu: 13.595 ± 0.155	3.855AlaMet: 3.855 ± 0.066	3.153AlaAsn: 3.153 ± 0.047	5.67AlaPro: 5.67 ± 0.085	4.669AlaGln: 4.669 ± 0.074	9.483AlaArg: 9.483 ± 0.1	6.569AlaSer: 6.569 ± 0.097	6.025AlaThr: 6.025 ± 0.078	8.204AlaVal: 8.204 ± 0.093	1.518AlaTrp: 1.518 ± 0.045	2.552AlaTyr: 2.552 ± 0.053	0.003AlaXaa: 0.003 ± 0.002
Cys	1.008CysAla: 1.008 ± 0.034	0.095CysCys: 0.095 ± 0.009	0.633CysAsp: 0.633 ± 0.024	0.522CysGlu: 0.522 ± 0.024	0.31CysPhe: 0.31 ± 0.02	0.855CysGly: 0.855 ± 0.03	0.243CysHis: 0.243 ± 0.021	0.374CysIle: 0.374 ± 0.019	0.197CysLys: 0.197 ± 0.014	0.689CysLeu: 0.689 ± 0.027	0.15CysMet: 0.15 ± 0.013	0.214CysAsn: 0.214 ± 0.016	0.421CysPro: 0.421 ± 0.019	0.215CysGln: 0.215 ± 0.016	0.528CysArg: 0.528 ± 0.024	0.469CysSer: 0.469 ± 0.024	0.399CysThr: 0.399 ± 0.024	0.558CysVal: 0.558 ± 0.026	0.11CysTrp: 0.11 ± 0.009	0.161CysTyr: 0.161 ± 0.013	0.0CysXaa: 0.0 ± 0.0
Asp	7.661AspAla: 7.661 ± 0.104	0.545AspCys: 0.545 ± 0.024	3.737AspAsp: 3.737 ± 0.075	4.378AspGlu: 4.378 ± 0.077	2.357AspPhe: 2.357 ± 0.056	5.955AspGly: 5.955 ± 0.089	1.371AspHis: 1.371 ± 0.042	3.069AspIle: 3.069 ± 0.061	1.811AspLys: 1.811 ± 0.053	6.037AspLeu: 6.037 ± 0.077	1.555AspMet: 1.555 ± 0.045	1.497AspAsn: 1.497 ± 0.044	3.801AspPro: 3.801 ± 0.07	1.762AspGln: 1.762 ± 0.041	4.884AspArg: 4.884 ± 0.064	2.617AspSer: 2.617 ± 0.055	3.065AspThr: 3.065 ± 0.058	3.918AspVal: 3.918 ± 0.062	1.199AspTrp: 1.199 ± 0.037	1.653AspTyr: 1.653 ± 0.042	0.0AspXaa: 0.0 ± 0.0
Glu	8.572GluAla: 8.572 ± 0.12	0.42GluCys: 0.42 ± 0.023	3.809GluAsp: 3.809 ± 0.07	4.148GluGlu: 4.148 ± 0.076	2.048GluPhe: 2.048 ± 0.047	5.324GluGly: 5.324 ± 0.063	1.317GluHis: 1.317 ± 0.042	3.37GluIle: 3.37 ± 0.069	2.27GluLys: 2.27 ± 0.06	6.045GluLeu: 6.045 ± 0.075	1.684GluMet: 1.684 ± 0.046	1.746GluAsn: 1.746 ± 0.042	3.033GluPro: 3.033 ± 0.059	2.612GluGln: 2.612 ± 0.056	5.681GluArg: 5.681 ± 0.078	2.672GluSer: 2.672 ± 0.057	3.637GluThr: 3.637 ± 0.064	4.043GluVal: 4.043 ± 0.068	0.967GluTrp: 0.967 ± 0.03	1.25GluTyr: 1.25 ± 0.04	0.002GluXaa: 0.002 ± 0.001
Phe	5.091PheAla: 5.091 ± 0.086	0.327PheCys: 0.327 ± 0.019	2.946PheAsp: 2.946 ± 0.065	2.397PheGlu: 2.397 ± 0.054	1.339PhePhe: 1.339 ± 0.042	3.747PheGly: 3.747 ± 0.069	0.677PheHis: 0.677 ± 0.029	1.515PheIle: 1.515 ± 0.037	0.809PheLys: 0.809 ± 0.033	3.144PheLeu: 3.144 ± 0.068	0.792PheMet: 0.792 ± 0.033	0.944PheAsn: 0.944 ± 0.031	1.531PhePro: 1.531 ± 0.041	0.847PheGln: 0.847 ± 0.029	2.086PheArg: 2.086 ± 0.042	2.058PheSer: 2.058 ± 0.056	2.04PheThr: 2.04 ± 0.046	2.774PheVal: 2.774 ± 0.051	0.525PheTrp: 0.525 ± 0.028	0.978PheTyr: 0.978 ± 0.032	0.0PheXaa: 0.0 ± 0.0
Gly	9.735GlyAla: 9.735 ± 0.112	0.825GlyCys: 0.825 ± 0.031	5.287GlyAsp: 5.287 ± 0.086	6.007GlyGlu: 6.007 ± 0.082	3.683GlyPhe: 3.683 ± 0.066	7.939GlyGly: 7.939 ± 0.157	1.8GlyHis: 1.8 ± 0.047	4.471GlyIle: 4.471 ± 0.077	3.249GlyLys: 3.249 ± 0.074	8.618GlyLeu: 8.618 ± 0.111	2.517GlyMet: 2.517 ± 0.058	2.301GlyAsn: 2.301 ± 0.051	3.514GlyPro: 3.514 ± 0.067	2.908GlyGln: 2.908 ± 0.059	5.997GlyArg: 5.997 ± 0.082	5.156GlySer: 5.156 ± 0.08	4.782GlyThr: 4.782 ± 0.087	5.939GlyVal: 5.939 ± 0.089	1.552GlyTrp: 1.552 ± 0.041	2.34GlyTyr: 2.34 ± 0.054	0.005GlyXaa: 0.005 ± 0.002
His	2.377HisAla: 2.377 ± 0.066	0.244HisCys: 0.244 ± 0.014	1.28HisAsp: 1.28 ± 0.041	1.189HisGlu: 1.189 ± 0.039	0.861HisPhe: 0.861 ± 0.03	1.96HisGly: 1.96 ± 0.051	0.521HisHis: 0.521 ± 0.025	0.863HisIle: 0.863 ± 0.032	0.463HisLys: 0.463 ± 0.021	1.816HisLeu: 1.816 ± 0.041	0.434HisMet: 0.434 ± 0.025	0.443HisAsn: 0.443 ± 0.021	1.261HisPro: 1.261 ± 0.039	0.512HisGln: 0.512 ± 0.023	1.422HisArg: 1.422 ± 0.038	0.961HisSer: 0.961 ± 0.034	0.793HisThr: 0.793 ± 0.03	1.406HisVal: 1.406 ± 0.038	0.364HisTrp: 0.364 ± 0.017	0.54HisTyr: 0.54 ± 0.024	0.0HisXaa: 0.0 ± 0.0
Ile	7.71IleAla: 7.71 ± 0.104	0.443IleCys: 0.443 ± 0.021	3.934IleAsp: 3.934 ± 0.064	3.946IleGlu: 3.946 ± 0.068	1.551IlePhe: 1.551 ± 0.044	5.156IleGly: 5.156 ± 0.078	0.842IleHis: 0.842 ± 0.031	1.971IleIle: 1.971 ± 0.052	1.164IleLys: 1.164 ± 0.036	3.839IleLeu: 3.839 ± 0.07	0.951IleMet: 0.951 ± 0.032	1.236IleAsn: 1.236 ± 0.039	2.251IlePro: 2.251 ± 0.046	1.01IleGln: 1.01 ± 0.034	3.109IleArg: 3.109 ± 0.058	2.483IleSer: 2.483 ± 0.048	2.423IleThr: 2.423 ± 0.053	3.896IleVal: 3.896 ± 0.068	0.626IleTrp: 0.626 ± 0.024	1.008IleTyr: 1.008 ± 0.035	0.001IleXaa: 0.001 ± 0.001
Lys	3.802LysAla: 3.802 ± 0.066	0.173LysCys: 0.173 ± 0.012	1.634LysAsp: 1.634 ± 0.049	1.41LysGlu: 1.41 ± 0.046	0.855LysPhe: 0.855 ± 0.033	2.515LysGly: 2.515 ± 0.055	0.639LysHis: 0.639 ± 0.025	1.381LysIle: 1.381 ± 0.04	1.186LysLys: 1.186 ± 0.052	3.103LysLeu: 3.103 ± 0.062	0.685LysMet: 0.685 ± 0.028	0.715LysAsn: 0.715 ± 0.028	1.902LysPro: 1.902 ± 0.047	0.982LysGln: 0.982 ± 0.036	2.403LysArg: 2.403 ± 0.053	1.479LysSer: 1.479 ± 0.042	1.605LysThr: 1.605 ± 0.045	2.178LysVal: 2.178 ± 0.051	0.389LysTrp: 0.389 ± 0.017	0.613LysTyr: 0.613 ± 0.024	0.003LysXaa: 0.003 ± 0.002
Leu	14.525LeuAla: 14.525 ± 0.163	0.733LeuCys: 0.733 ± 0.029	6.447LeuAsp: 6.447 ± 0.101	5.745LeuGlu: 5.745 ± 0.082	3.574LeuPhe: 3.574 ± 0.069	8.413LeuGly: 8.413 ± 0.114	1.729LeuHis: 1.729 ± 0.045	4.253LeuIle: 4.253 ± 0.063	2.765LeuLys: 2.765 ± 0.056	9.315LeuLeu: 9.315 ± 0.136	2.093LeuMet: 2.093 ± 0.05	2.199LeuAsn: 2.199 ± 0.045	5.441LeuPro: 5.441 ± 0.09	2.517LeuGln: 2.517 ± 0.045	6.824LeuArg: 6.824 ± 0.093	5.589LeuSer: 5.589 ± 0.081	5.261LeuThr: 5.261 ± 0.08	7.805LeuVal: 7.805 ± 0.101	1.203LeuTrp: 1.203 ± 0.036	1.909LeuTyr: 1.909 ± 0.049	0.001LeuXaa: 0.001 ± 0.001
Met	3.255MetAla: 3.255 ± 0.058	0.16MetCys: 0.16 ± 0.013	1.236MetAsp: 1.236 ± 0.037	1.283MetGlu: 1.283 ± 0.033	0.678MetPhe: 0.678 ± 0.03	2.047MetGly: 2.047 ± 0.053	0.476MetHis: 0.476 ± 0.021	1.283MetIle: 1.283 ± 0.042	0.927MetLys: 0.927 ± 0.034	2.576MetLeu: 2.576 ± 0.058	0.644MetMet: 0.644 ± 0.026	0.734MetAsn: 0.734 ± 0.027	1.381MetPro: 1.381 ± 0.041	0.843MetGln: 0.843 ± 0.03	1.848MetArg: 1.848 ± 0.039	1.457MetSer: 1.457 ± 0.04	1.654MetThr: 1.654 ± 0.037	1.703MetVal: 1.703 ± 0.047	0.256MetTrp: 0.256 ± 0.02	0.29MetTyr: 0.29 ± 0.017	0.0MetXaa: 0.0 ± 0.0
Asn	3.158AsnAla: 3.158 ± 0.054	0.251AsnCys: 0.251 ± 0.018	1.426AsnAsp: 1.426 ± 0.036	1.328AsnGlu: 1.328 ± 0.041	1.007AsnPhe: 1.007 ± 0.036	2.361AsnGly: 2.361 ± 0.051	0.511AsnHis: 0.511 ± 0.02	1.338AsnIle: 1.338 ± 0.037	0.586AsnLys: 0.586 ± 0.024	2.4AsnLeu: 2.4 ± 0.055	0.518AsnMet: 0.518 ± 0.024	0.619AsnAsn: 0.619 ± 0.032	1.733AsnPro: 1.733 ± 0.046	0.74AsnGln: 0.74 ± 0.029	1.932AsnArg: 1.932 ± 0.045	1.246AsnSer: 1.246 ± 0.039	1.132AsnThr: 1.132 ± 0.043	1.774AsnVal: 1.774 ± 0.042	0.4AsnTrp: 0.4 ± 0.02	0.681AsnTyr: 0.681 ± 0.032	0.002AsnXaa: 0.002 ± 0.001
Pro	6.222ProAla: 6.222 ± 0.088	0.313ProCys: 0.313 ± 0.021	3.802ProAsp: 3.802 ± 0.07	4.076ProGlu: 4.076 ± 0.068	1.876ProPhe: 1.876 ± 0.038	4.523ProGly: 4.523 ± 0.071	1.01ProHis: 1.01 ± 0.032	2.348ProIle: 2.348 ± 0.046	1.373ProLys: 1.373 ± 0.039	4.793ProLeu: 4.793 ± 0.076	1.085ProMet: 1.085 ± 0.034	1.134ProAsn: 1.134 ± 0.031	2.349ProPro: 2.349 ± 0.066	1.819ProGln: 1.819 ± 0.039	3.009ProArg: 3.009 ± 0.072	2.496ProSer: 2.496 ± 0.052	2.372ProThr: 2.372 ± 0.043	4.05ProVal: 4.05 ± 0.075	0.666ProTrp: 0.666 ± 0.03	1.098ProTyr: 1.098 ± 0.027	0.002ProXaa: 0.002 ± 0.001
Gln	4.025GlnAla: 4.025 ± 0.067	0.247GlnCys: 0.247 ± 0.016	1.592GlnAsp: 1.592 ± 0.039	1.592GlnGlu: 1.592 ± 0.04	1.202GlnPhe: 1.202 ± 0.037	2.53GlnGly: 2.53 ± 0.052	0.607GlnHis: 0.607 ± 0.024	1.743GlnIle: 1.743 ± 0.044	0.869GlnLys: 0.869 ± 0.031	3.159GlnLeu: 3.159 ± 0.054	0.86GlnMet: 0.86 ± 0.027	0.775GlnAsn: 0.775 ± 0.029	1.667GlnPro: 1.667 ± 0.038	1.262GlnGln: 1.262 ± 0.047	2.641GlnArg: 2.641 ± 0.051	1.715GlnSer: 1.715 ± 0.041	1.646GlnThr: 1.646 ± 0.042	2.34GlnVal: 2.34 ± 0.047	0.412GlnTrp: 0.412 ± 0.019	0.662GlnTyr: 0.662 ± 0.026	0.001GlnXaa: 0.001 ± 0.001
Arg	8.178ArgAla: 8.178 ± 0.106	0.483ArgCys: 0.483 ± 0.023	4.476ArgAsp: 4.476 ± 0.066	5.022ArgGlu: 5.022 ± 0.074	3.01ArgPhe: 3.01 ± 0.055	5.168ArgGly: 5.168 ± 0.073	1.585ArgHis: 1.585 ± 0.045	4.173ArgIle: 4.173 ± 0.063	2.272ArgLys: 2.272 ± 0.054	7.693ArgLeu: 7.693 ± 0.114	1.965ArgMet: 1.965 ± 0.048	1.903ArgAsn: 1.903 ± 0.045	3.279ArgPro: 3.279 ± 0.054	2.478ArgGln: 2.478 ± 0.052	5.274ArgArg: 5.274 ± 0.075	3.752ArgSer: 3.752 ± 0.064	3.358ArgThr: 3.358 ± 0.062	4.75ArgVal: 4.75 ± 0.065	1.199ArgTrp: 1.199 ± 0.039	1.808ArgTyr: 1.808 ± 0.042	0.001ArgXaa: 0.001 ± 0.001
Ser	6.266SerAla: 6.266 ± 0.085	0.422SerCys: 0.422 ± 0.021	3.357SerAsp: 3.357 ± 0.06	3.156SerGlu: 3.156 ± 0.058	2.17SerPhe: 2.17 ± 0.042	5.33SerGly: 5.33 ± 0.08	0.955SerHis: 0.955 ± 0.031	2.471SerIle: 2.471 ± 0.057	1.461SerLys: 1.461 ± 0.039	5.156SerLeu: 5.156 ± 0.08	1.233SerMet: 1.233 ± 0.034	1.362SerAsn: 1.362 ± 0.041	2.653SerPro: 2.653 ± 0.056	1.659SerGln: 1.659 ± 0.043	3.508SerArg: 3.508 ± 0.07	2.723SerSer: 2.723 ± 0.064	2.44SerThr: 2.44 ± 0.051	3.596SerVal: 3.596 ± 0.072	0.783SerTrp: 0.783 ± 0.028	1.355SerTyr: 1.355 ± 0.039	0.001SerXaa: 0.001 ± 0.001
Thr	6.1ThrAla: 6.1 ± 0.081	0.416ThrCys: 0.416 ± 0.021	2.945ThrAsp: 2.945 ± 0.057	2.842ThrGlu: 2.842 ± 0.061	1.751ThrPhe: 1.751 ± 0.04	5.34ThrGly: 5.34 ± 0.091	0.967ThrHis: 0.967 ± 0.031	2.801ThrIle: 2.801 ± 0.056	1.325ThrLys: 1.325 ± 0.041	5.509ThrLeu: 5.509 ± 0.075	1.198ThrMet: 1.198 ± 0.038	1.28ThrAsn: 1.28 ± 0.039	3.129ThrPro: 3.129 ± 0.059	1.439ThrGln: 1.439 ± 0.037	3.413ThrArg: 3.413 ± 0.063	2.59ThrSer: 2.59 ± 0.051	2.446ThrThr: 2.446 ± 0.06	4.065ThrVal: 4.065 ± 0.068	0.567ThrTrp: 0.567 ± 0.028	1.188ThrTyr: 1.188 ± 0.035	0.001ThrXaa: 0.001 ± 0.001
Val	8.936ValAla: 8.936 ± 0.107	0.571ValCys: 0.571 ± 0.026	4.54ValAsp: 4.54 ± 0.073	5.06ValGlu: 5.06 ± 0.078	2.454ValPhe: 2.454 ± 0.058	5.463ValGly: 5.463 ± 0.078	1.312ValHis: 1.312 ± 0.04	3.7ValIle: 3.7 ± 0.067	1.892ValLys: 1.892 ± 0.054	6.899ValLeu: 6.899 ± 0.09	1.751ValMet: 1.751 ± 0.047	1.855ValAsn: 1.855 ± 0.044	3.714ValPro: 3.714 ± 0.058	1.969ValGln: 1.969 ± 0.044	4.672ValArg: 4.672 ± 0.07	3.931ValSer: 3.931 ± 0.064	4.326ValThr: 4.326 ± 0.063	5.395ValVal: 5.395 ± 0.094	0.906ValTrp: 0.906 ± 0.03	1.331ValTyr: 1.331 ± 0.036	0.002ValXaa: 0.002 ± 0.001
Trp	1.35TrpAla: 1.35 ± 0.04	0.129TrpCys: 0.129 ± 0.012	0.797TrpAsp: 0.797 ± 0.03	0.686TrpGlu: 0.686 ± 0.023	0.63TrpPhe: 0.63 ± 0.029	1.008TrpGly: 1.008 ± 0.033	0.353TrpHis: 0.353 ± 0.016	0.72TrpIle: 0.72 ± 0.029	0.442TrpLys: 0.442 ± 0.021	1.802TrpLeu: 1.802 ± 0.051	0.349TrpMet: 0.349 ± 0.02	0.451TrpAsn: 0.451 ± 0.02	0.688TrpPro: 0.688 ± 0.027	0.647TrpGln: 0.647 ± 0.028	1.224TrpArg: 1.224 ± 0.038	0.869TrpSer: 0.869 ± 0.032	0.77TrpThr: 0.77 ± 0.03	0.801TrpVal: 0.801 ± 0.031	0.246TrpTrp: 0.246 ± 0.02	0.295TrpTyr: 0.295 ± 0.019	0.001TrpXaa: 0.001 ± 0.001
Tyr	2.638TyrAla: 2.638 ± 0.051	0.242TyrCys: 0.242 ± 0.015	1.595TyrAsp: 1.595 ± 0.045	1.36TyrGlu: 1.36 ± 0.036	0.907TyrPhe: 0.907 ± 0.035	2.156TyrGly: 2.156 ± 0.052	0.483TyrHis: 0.483 ± 0.023	0.898TyrIle: 0.898 ± 0.031	0.539TyrLys: 0.539 ± 0.027	2.153TyrLeu: 2.153 ± 0.049	0.411TyrMet: 0.411 ± 0.023	0.585TyrAsn: 0.585 ± 0.027	0.998TyrPro: 0.998 ± 0.036	0.655TyrGln: 0.655 ± 0.024	1.884TyrArg: 1.884 ± 0.045	1.219TyrSer: 1.219 ± 0.037	1.137TyrThr: 1.137 ± 0.036	1.484TyrVal: 1.484 ± 0.042	0.369TyrTrp: 0.369 ± 0.02	0.628TyrTyr: 0.628 ± 0.023	0.001TyrXaa: 0.001 ± 0.001
Xaa	0.002XaaAla: 0.002 ± 0.001	0.0XaaCys: 0.0 ± 0.0	0.001XaaAsp: 0.001 ± 0.001	0.003XaaGlu: 0.003 ± 0.002	0.001XaaPhe: 0.001 ± 0.001	0.002XaaGly: 0.002 ± 0.001	0.0XaaHis: 0.0 ± 0.0	0.001XaaIle: 0.001 ± 0.001	0.002XaaLys: 0.002 ± 0.001	0.001XaaLeu: 0.001 ± 0.001	0.0XaaMet: 0.0 ± 0.0	0.001XaaAsn: 0.001 ± 0.001	0.001XaaPro: 0.001 ± 0.001	0.001XaaGln: 0.001 ± 0.001	0.003XaaArg: 0.003 ± 0.002	0.005XaaSer: 0.005 ± 0.002	0.001XaaThr: 0.001 ± 0.001	0.001XaaVal: 0.001 ± 0.001	0.001XaaTrp: 0.001 ± 0.001	0.0XaaTyr: 0.0 ± 0.0	0.001XaaXaa: 0.001 ± 0.001

Statistics based on 3091 proteins (982353 amino acids)

Note: The error has been estimated with the bootstraping (x100) at the protein level

Above dipeptide statistics (among other stats for this proteome) you can download from this CSV file

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944

Contact: Lukasz P. Kozlowski

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