Human adenovirus B serotype 3 (HAdV-3) (Human adenovirus 3)
Average proteome isoelectric point is 6.76
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 46 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q2Y0F3|Q2Y0F3_ADE03 Isoform of Q2Y0F6 11 kDa protein OS=Human adenovirus B serotype 3 OX=45659 GN=E4 PE=4 SV=1
MM1 pKa = 7.12 TLVSFILQTNSKK13 pKa = 8.71 LTMLEE18 pKa = 4.05 RR19 pKa = 11.84 SAVTYY24 pKa = 7.35 CICIPTVLNVSLEE37 pKa = 4.12 NFSFIQFLQEE47 pKa = 3.9 TLPDD51 pKa = 4.12 YY52 pKa = 10.8 ISSNFDD58 pKa = 4.36 DD59 pKa = 3.7 ITGGSQYY66 pKa = 11.16 AYY68 pKa = 10.73 SNLVFAGANWGGLRR82 pKa = 11.84 LYY84 pKa = 9.61 CTVASPAIVPGGLLAKK100 pKa = 10.48 QFGDD104 pKa = 3.16 NMRR107 pKa = 11.84 EE108 pKa = 3.87 YY109 pKa = 10.83 LQLEE113 pKa = 4.09 LRR115 pKa = 11.84 EE116 pKa = 4.01 EE117 pKa = 4.1 LRR119 pKa = 11.84 AKK121 pKa = 10.56 GMSIDD126 pKa = 4.34 DD127 pKa = 5.14 AILNLLQVTQEE138 pKa = 3.78 QDD140 pKa = 2.96 ILSLL144 pKa = 3.83
Molecular weight: 16.03 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 4.025
IPC2_protein 4.355
IPC_protein 4.228
Toseland 4.05
ProMoST 4.355
Dawson 4.19
Bjellqvist 4.342
Wikipedia 4.075
Rodwell 4.062
Grimsley 3.973
Solomon 4.177
Lehninger 4.126
Nozaki 4.304
DTASelect 4.457
Thurlkill 4.088
EMBOSS 4.088
Sillero 4.342
Patrickios 1.977
IPC_peptide 4.177
IPC2_peptide 4.329
IPC2.peptide.svr19 4.243
Protein with the highest isoelectric point:
>tr|Q2Y0H7|Q2Y0H7_ADE03 Isoform of Q2Y0H9 L2 protein pV OS=Human adenovirus B serotype 3 OX=45659 GN=L2 PE=3 SV=1
MM1 pKa = 7.77 ALTCRR6 pKa = 11.84 LRR8 pKa = 11.84 VPITGYY14 pKa = 9.94 RR15 pKa = 11.84 GRR17 pKa = 11.84 NSRR20 pKa = 11.84 RR21 pKa = 11.84 RR22 pKa = 11.84 RR23 pKa = 11.84 MLGRR27 pKa = 11.84 GMRR30 pKa = 11.84 RR31 pKa = 11.84 HH32 pKa = 5.65 RR33 pKa = 11.84 RR34 pKa = 11.84 RR35 pKa = 11.84 RR36 pKa = 11.84 AISRR40 pKa = 11.84 RR41 pKa = 11.84 LGGGFLPALIPIIAAAIGAIPGIASVAVQASQRR74 pKa = 11.84 HH75 pKa = 4.3
Molecular weight: 8.33 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.434
IPC2_protein 11.067
IPC_protein 12.544
Toseland 12.705
ProMoST 13.203
Dawson 12.705
Bjellqvist 12.705
Wikipedia 13.173
Rodwell 12.193
Grimsley 12.735
Solomon 13.203
Lehninger 13.1
Nozaki 12.705
DTASelect 12.705
Thurlkill 12.705
EMBOSS 13.203
Sillero 12.705
Patrickios 11.93
IPC_peptide 13.203
IPC2_peptide 12.193
IPC2.peptide.svr19 9.148
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
21
25
46
12716
57
1122
276.4
31.06
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.18 ± 0.342
1.95 ± 0.249
5.025 ± 0.219
6.221 ± 0.356
3.688 ± 0.235
6.26 ± 0.305
2.375 ± 0.161
4.821 ± 0.265
4.042 ± 0.33
9.28 ± 0.385
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.792 ± 0.164
4.388 ± 0.346
6.795 ± 0.29
4.278 ± 0.242
7.148 ± 0.483
7.101 ± 0.393
6.158 ± 0.293
5.922 ± 0.221
1.29 ± 0.116
3.287 ± 0.249
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here