Capybara microvirus Cap1_SP_52
Average proteome isoelectric point is 6.12
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 5 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A4V1FVM6|A0A4V1FVM6_9VIRU Major capsid protein OS=Capybara microvirus Cap1_SP_52 OX=2584782 PE=3 SV=1
MM1 pKa = 7.6 SKK3 pKa = 10.62 NPFNIKK9 pKa = 9.79 DD10 pKa = 3.61 VRR12 pKa = 11.84 NHH14 pKa = 5.54 VSHH17 pKa = 6.96 NGFDD21 pKa = 3.72 LSEE24 pKa = 4.43 KK25 pKa = 10.7 KK26 pKa = 10.77 LFTAKK31 pKa = 10.32 CGEE34 pKa = 4.76 LLPIMHH40 pKa = 6.52 RR41 pKa = 11.84 TVMPGDD47 pKa = 3.51 KK48 pKa = 10.42 FRR50 pKa = 11.84 ITKK53 pKa = 9.15 QHH55 pKa = 5.58 LTRR58 pKa = 11.84 TIPVDD63 pKa = 3.12 TAAYY67 pKa = 6.79 TRR69 pKa = 11.84 LRR71 pKa = 11.84 EE72 pKa = 3.86 HH73 pKa = 7.01 FEE75 pKa = 4.27 FFFVPYY81 pKa = 10.04 DD82 pKa = 3.47 QLYY85 pKa = 9.76 RR86 pKa = 11.84 PANQVLEE93 pKa = 4.22 NLSGLSSTRR102 pKa = 11.84 ATSLVEE108 pKa = 3.68 YY109 pKa = 8.83 TQVNEE114 pKa = 3.86 YY115 pKa = 10.19 LPNIVYY121 pKa = 9.86 SAPQSQEE128 pKa = 3.26 TSYY131 pKa = 11.27 LALFPSGTQSFDD143 pKa = 2.9 NTTPKK148 pKa = 10.69 PEE150 pKa = 4.0 FRR152 pKa = 11.84 NDD154 pKa = 4.37 LITSLASMLNNVDD167 pKa = 2.82 VSSRR171 pKa = 11.84 YY172 pKa = 10.03 NEE174 pKa = 3.91 VGRR177 pKa = 11.84 NPIYY181 pKa = 10.34 ATIKK185 pKa = 9.41 LLNYY189 pKa = 10.16 LGYY192 pKa = 10.33 GYY194 pKa = 10.46 HH195 pKa = 7.22 ADD197 pKa = 3.42 SFLNEE202 pKa = 3.69 IQPYY206 pKa = 8.86 ISKK209 pKa = 10.68 DD210 pKa = 3.14 GEE212 pKa = 4.43 RR213 pKa = 11.84 ILGKK217 pKa = 10.53 SFDD220 pKa = 3.78 FGVAKK225 pKa = 9.19 TQTRR229 pKa = 11.84 LSLLPLAAYY238 pKa = 9.28 QKK240 pKa = 10.47 IYY242 pKa = 10.41 FDD244 pKa = 4.05 YY245 pKa = 10.76 YY246 pKa = 11.14 RR247 pKa = 11.84 NEE249 pKa = 3.7 QWEE252 pKa = 4.34 KK253 pKa = 11.49 DD254 pKa = 3.66 NPSCYY259 pKa = 10.36 NFDD262 pKa = 3.32 YY263 pKa = 10.58 MMPHH267 pKa = 6.74 INVDD271 pKa = 3.22 SSLNNVMQTTTTQCTLLKK289 pKa = 8.76 THH291 pKa = 7.2 ASFGSSPSVSFIDD304 pKa = 3.9 FAFNNFATLRR314 pKa = 11.84 YY315 pKa = 9.84 RR316 pKa = 11.84 NFKK319 pKa = 10.05 KK320 pKa = 10.65 DD321 pKa = 3.6 LFFGCLPRR329 pKa = 11.84 PQAGNTTFVPTSPIFKK345 pKa = 9.36 ATDD348 pKa = 3.24 TEE350 pKa = 4.67 FPEE353 pKa = 3.91 NGYY356 pKa = 9.94 NRR358 pKa = 11.84 DD359 pKa = 3.06 VNTTYY364 pKa = 10.45 FRR366 pKa = 11.84 NANALFQSFPRR377 pKa = 11.84 LQNLTPEE384 pKa = 4.1 QQNIEE389 pKa = 4.15 GYY391 pKa = 10.7 LYY393 pKa = 10.9 DD394 pKa = 3.67 GSNYY398 pKa = 7.28 VTPATGGFAIQDD410 pKa = 3.51 FRR412 pKa = 11.84 IGMALQRR419 pKa = 11.84 YY420 pKa = 8.32 RR421 pKa = 11.84 EE422 pKa = 4.21 VVGSARR428 pKa = 11.84 HH429 pKa = 6.01 DD430 pKa = 3.52 FVNEE434 pKa = 3.74 TEE436 pKa = 4.08 RR437 pKa = 11.84 QFGVKK442 pKa = 9.55 VPQYY446 pKa = 10.44 LSHH449 pKa = 6.97 KK450 pKa = 9.24 VQFLGEE456 pKa = 3.9 FSNWIGISAIVNNNLDD472 pKa = 4.03 TADD475 pKa = 3.6 STTDD479 pKa = 2.81 IKK481 pKa = 11.38 GIGVGSSSDD490 pKa = 3.05 NDD492 pKa = 4.27 AINFKK497 pKa = 11.0 AMEE500 pKa = 4.58 FGIIMCIYY508 pKa = 10.03 SVEE511 pKa = 4.07 PQVDD515 pKa = 3.72 YY516 pKa = 11.32 EE517 pKa = 4.5 FDD519 pKa = 3.51 GFAPDD524 pKa = 4.49 LLKK527 pKa = 11.1 CHH529 pKa = 6.76 ASSFANPAFDD539 pKa = 4.22 NIGYY543 pKa = 9.92 EE544 pKa = 4.47 PIPKK548 pKa = 10.12 ALISDD553 pKa = 3.78 EE554 pKa = 4.4 QYY556 pKa = 11.56 GSDD559 pKa = 3.03 IDD561 pKa = 4.18 MPNQYY566 pKa = 10.2 HH567 pKa = 5.49 SQNHH571 pKa = 5.91 LGFNVRR577 pKa = 11.84 YY578 pKa = 8.53 WDD580 pKa = 3.7 YY581 pKa = 10.73 KK582 pKa = 9.64 TGFDD586 pKa = 3.55 NVLGAFRR593 pKa = 11.84 EE594 pKa = 4.42 TLVTWVAPLNFNTLLSQLVPNFGDD618 pKa = 3.64 ASDD621 pKa = 4.66 ANTARR626 pKa = 11.84 VNSHH630 pKa = 5.76 ISANGNMKK638 pKa = 9.93 YY639 pKa = 10.37 HH640 pKa = 6.54 NNPNYY645 pKa = 10.37 GYY647 pKa = 9.99 SQFPGYY653 pKa = 10.35 GYY655 pKa = 9.6 PIINYY660 pKa = 9.57 AVFKK664 pKa = 10.55 INPSLLDD671 pKa = 3.64 TIFFAQVNDD680 pKa = 3.8 LTEE683 pKa = 4.1 TDD685 pKa = 3.56 QFLVNCNLSIKK696 pKa = 10.3 ALRR699 pKa = 11.84 PLSWSGLPYY708 pKa = 10.89
Molecular weight: 80.4 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 5.321
IPC2_protein 5.397
IPC_protein 5.423
Toseland 5.715
ProMoST 5.703
Dawson 5.588
Bjellqvist 5.639
Wikipedia 5.563
Rodwell 5.55
Grimsley 5.83
Solomon 5.588
Lehninger 5.575
Nozaki 5.817
DTASelect 6.008
Thurlkill 5.906
EMBOSS 5.881
Sillero 5.906
Patrickios 3.414
IPC_peptide 5.601
IPC2_peptide 5.906
IPC2.peptide.svr19 5.865
Protein with the highest isoelectric point:
>tr|A0A4P8W3R5|A0A4P8W3R5_9VIRU Uncharacterized protein OS=Capybara microvirus Cap1_SP_52 OX=2584782 PE=4 SV=1
MM1 pKa = 7.36 SLCLKK6 pKa = 9.77 PIKK9 pKa = 9.74 IWQNNRR15 pKa = 11.84 EE16 pKa = 4.07 RR17 pKa = 11.84 TVSCGHH23 pKa = 6.88 CAHH26 pKa = 7.49 CINAKK31 pKa = 10.03 HH32 pKa = 6.11 IRR34 pKa = 11.84 NKK36 pKa = 10.24 QLVEE40 pKa = 4.17 LEE42 pKa = 4.85 SNSHH46 pKa = 6.51 PYY48 pKa = 10.68 EE49 pKa = 4.17 LFCTLTYY56 pKa = 11.0 DD57 pKa = 4.07 DD58 pKa = 3.8 YY59 pKa = 11.5 HH60 pKa = 7.92 VPKK63 pKa = 10.47 VHH65 pKa = 6.62 YY66 pKa = 10.43 SHH68 pKa = 6.99 TNKK71 pKa = 10.2 LSSIEE76 pKa = 4.14 SEE78 pKa = 5.05 DD79 pKa = 2.97 IFEE82 pKa = 4.35 FTNSRR87 pKa = 11.84 FKK89 pKa = 11.49 DD90 pKa = 4.09 EE91 pKa = 4.14 VCKK94 pKa = 10.92 GRR96 pKa = 11.84 FAVPNYY102 pKa = 9.04 YY103 pKa = 10.66 LKK105 pKa = 10.68 DD106 pKa = 3.39 VHH108 pKa = 7.11 SINMRR113 pKa = 11.84 QQLFNGEE120 pKa = 4.08 IRR122 pKa = 11.84 TLNYY126 pKa = 10.08 VDD128 pKa = 4.48 LRR130 pKa = 11.84 LYY132 pKa = 9.55 LQRR135 pKa = 11.84 VRR137 pKa = 11.84 QALRR141 pKa = 11.84 RR142 pKa = 11.84 SLGTNVRR149 pKa = 11.84 FYY151 pKa = 11.6 AVGEE155 pKa = 4.08 YY156 pKa = 10.64 GPKK159 pKa = 9.89 KK160 pKa = 10.25 YY161 pKa = 9.92 RR162 pKa = 11.84 PHH164 pKa = 6.62 YY165 pKa = 10.21 HH166 pKa = 6.68 IIFWCDD172 pKa = 3.03 DD173 pKa = 3.56 LASRR177 pKa = 11.84 DD178 pKa = 3.66 WLLVNALSYY187 pKa = 9.69 WPYY190 pKa = 8.59 GTQIEE195 pKa = 4.49 IEE197 pKa = 4.14 QSRR200 pKa = 11.84 KK201 pKa = 10.19 KK202 pKa = 10.47 LDD204 pKa = 3.44 SYY206 pKa = 11.2 LAGYY210 pKa = 9.46 VNSEE214 pKa = 3.93 TMLPPFFSCLKK225 pKa = 10.53 SPMRR229 pKa = 11.84 PKK231 pKa = 10.4 AYY233 pKa = 9.82 HH234 pKa = 6.48 SNFLGQTYY242 pKa = 9.34 PFAYY246 pKa = 9.64 LSVMFHH252 pKa = 6.89 SDD254 pKa = 3.02 PGKK257 pKa = 10.48 KK258 pKa = 9.64 FEE260 pKa = 5.25 LFKK263 pKa = 8.64 TTKK266 pKa = 10.33 GIYY269 pKa = 8.8 DD270 pKa = 4.89 TIPKK274 pKa = 10.33 AIFDD278 pKa = 3.66 YY279 pKa = 10.19 FYY281 pKa = 11.16 RR282 pKa = 11.84 KK283 pKa = 9.69 LPNYY287 pKa = 9.88 QNLDD291 pKa = 3.34 NHH293 pKa = 6.52 EE294 pKa = 4.17 KK295 pKa = 7.1 TTLYY299 pKa = 11.27 KK300 pKa = 10.55 LFNVMYY306 pKa = 9.8 DD307 pKa = 3.48 YY308 pKa = 10.79 RR309 pKa = 11.84 QKK311 pKa = 10.79 QVGEE315 pKa = 4.27 NIPLEE320 pKa = 4.18 NYY322 pKa = 9.6 KK323 pKa = 10.51 FSAFLEE329 pKa = 4.16 DD330 pKa = 4.62 LKK332 pKa = 11.35 DD333 pKa = 3.86 FEE335 pKa = 5.54 RR336 pKa = 11.84 NEE338 pKa = 4.01 FYY340 pKa = 10.35 IINLLGIDD348 pKa = 5.1 DD349 pKa = 4.09 FFEE352 pKa = 4.1 YY353 pKa = 10.65 LKK355 pKa = 10.41 PLEE358 pKa = 4.03 VNYY361 pKa = 10.2 EE362 pKa = 4.15 YY363 pKa = 11.35 KK364 pKa = 10.67 CVGEE368 pKa = 4.11 NLLTYY373 pKa = 9.91 RR374 pKa = 11.84 WHH376 pKa = 7.08 PKK378 pKa = 10.03 LISLYY383 pKa = 10.01 RR384 pKa = 11.84 VFSIGKK390 pKa = 8.78 FYY392 pKa = 10.73 FWLSDD397 pKa = 3.18 ITSINYY403 pKa = 7.41 NTLIDD408 pKa = 3.66 KK409 pKa = 10.11 VIYY412 pKa = 10.68 YY413 pKa = 9.89 FDD415 pKa = 4.27 KK416 pKa = 10.87 EE417 pKa = 4.31 QPQQMLANFFSNIEE431 pKa = 4.14 TLPDD435 pKa = 3.77 ISPQDD440 pKa = 3.48 LSFLYY445 pKa = 10.93 NLDD448 pKa = 4.71 DD449 pKa = 5.05 DD450 pKa = 5.12 YY451 pKa = 11.89 KK452 pKa = 11.19 DD453 pKa = 3.3 SKK455 pKa = 11.06 LYY457 pKa = 10.69 QCIKK461 pKa = 7.87 THH463 pKa = 6.71 AIEE466 pKa = 4.19 VANDD470 pKa = 3.33 NVKK473 pKa = 10.28 HH474 pKa = 6.11 KK475 pKa = 10.87 KK476 pKa = 10.18 EE477 pKa = 3.86 NDD479 pKa = 3.38 GLKK482 pKa = 9.93 II483 pKa = 3.77
Molecular weight: 57.7 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 7.031
IPC2_protein 7.278
IPC_protein 7.176
Toseland 7.0
ProMoST 7.717
Dawson 7.849
Bjellqvist 8.126
Wikipedia 7.79
Rodwell 7.863
Grimsley 7.102
Solomon 7.892
Lehninger 7.907
Nozaki 8.214
DTASelect 7.98
Thurlkill 8.009
EMBOSS 8.083
Sillero 8.287
Patrickios 3.605
IPC_peptide 7.892
IPC2_peptide 7.263
IPC2.peptide.svr19 7.351
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
5
0
5
1793
90
708
358.6
41.36
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
5.354 ± 1.119
1.171 ± 0.404
6.023 ± 0.635
4.964 ± 0.65
5.968 ± 0.864
4.518 ± 0.787
2.119 ± 0.467
6.247 ± 0.869
5.633 ± 1.045
9.314 ± 0.601
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
1.952 ± 0.343
8.31 ± 0.644
4.239 ± 0.811
5.02 ± 0.969
4.629 ± 0.288
7.808 ± 0.614
5.521 ± 0.681
4.462 ± 0.656
0.892 ± 0.15
5.856 ± 0.902
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here