Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas)

Taxonomy: cellular organisms; Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Anaplasmataceae; Ehrlichia; canis group; Ehrlichia chaffeensis

Average proteome isoelectric point is 6.95

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 1100 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|Q2GFX2|Q2GFX2_EHRCR Uncharacterized protein OS=Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas) OX=205920 GN=ECH_0866 PE=4 SV=1
MM1 pKa = 7.46NNTHH5 pKa = 5.63NTNNVVNQVQDD16 pKa = 3.24IAQDD20 pKa = 3.43NHH22 pKa = 7.96ISDD25 pKa = 4.08NNANEE30 pKa = 3.82ARR32 pKa = 11.84EE33 pKa = 3.99VLAAHH38 pKa = 6.9AAEE41 pKa = 4.39NNANEE46 pKa = 4.12IRR48 pKa = 11.84EE49 pKa = 4.12VLHH52 pKa = 7.43DD53 pKa = 4.19YY54 pKa = 11.37SLDD57 pKa = 3.7DD58 pKa = 4.58YY59 pKa = 11.72AIDD62 pKa = 3.69DD63 pKa = 4.27HH64 pKa = 7.86NIGGNAEE71 pKa = 4.04AQDD74 pKa = 4.7DD75 pKa = 4.41YY76 pKa = 12.12SLDD79 pKa = 3.91DD80 pKa = 4.08YY81 pKa = 11.81AIDD84 pKa = 5.06DD85 pKa = 4.07YY86 pKa = 11.87NIGGNAEE93 pKa = 4.05VQDD96 pKa = 3.78NHH98 pKa = 8.18ISDD101 pKa = 4.1NNTNEE106 pKa = 3.65ARR108 pKa = 11.84EE109 pKa = 4.02VLLAHH114 pKa = 7.02AAEE117 pKa = 4.42NNANEE122 pKa = 4.11IRR124 pKa = 11.84EE125 pKa = 4.02VLYY128 pKa = 10.54DD129 pKa = 3.76YY130 pKa = 11.6SLDD133 pKa = 4.07DD134 pKa = 4.54YY135 pKa = 11.68AIDD138 pKa = 4.31DD139 pKa = 4.31HH140 pKa = 8.24NIDD143 pKa = 4.16GNAEE147 pKa = 3.96VQDD150 pKa = 3.68NHH152 pKa = 8.18ISDD155 pKa = 4.1NNTNEE160 pKa = 3.65ARR162 pKa = 11.84EE163 pKa = 4.02VLLAHH168 pKa = 7.02AAEE171 pKa = 4.42NNANEE176 pKa = 4.11IRR178 pKa = 11.84EE179 pKa = 4.02VLYY182 pKa = 10.54DD183 pKa = 3.76YY184 pKa = 11.6SLDD187 pKa = 4.07DD188 pKa = 4.54YY189 pKa = 11.68AIDD192 pKa = 3.69DD193 pKa = 4.27HH194 pKa = 7.86NIGGNAEE201 pKa = 4.04AQDD204 pKa = 4.7DD205 pKa = 4.41YY206 pKa = 12.12SLDD209 pKa = 3.91DD210 pKa = 4.08YY211 pKa = 11.81AIDD214 pKa = 5.06DD215 pKa = 4.07YY216 pKa = 11.87NIGGNAEE223 pKa = 3.99AQDD226 pKa = 4.11NYY228 pKa = 11.59SLDD231 pKa = 4.13DD232 pKa = 3.91YY233 pKa = 11.73AIDD236 pKa = 5.06DD237 pKa = 4.07YY238 pKa = 11.87NIGGNAEE245 pKa = 4.05VQDD248 pKa = 3.78NHH250 pKa = 8.18ISDD253 pKa = 4.0NNTNEE258 pKa = 3.8TRR260 pKa = 11.84EE261 pKa = 4.1ALAAHH266 pKa = 6.58AAEE269 pKa = 4.45NNANEE274 pKa = 4.05IRR276 pKa = 11.84EE277 pKa = 4.01VLYY280 pKa = 10.83NYY282 pKa = 10.84SLDD285 pKa = 4.41DD286 pKa = 4.1YY287 pKa = 11.56AIDD290 pKa = 4.31DD291 pKa = 4.31HH292 pKa = 8.24NIDD295 pKa = 4.16GNAEE299 pKa = 3.96VQDD302 pKa = 3.68NHH304 pKa = 8.18ISDD307 pKa = 4.0NNTNEE312 pKa = 3.8TRR314 pKa = 11.84EE315 pKa = 4.1ALAAHH320 pKa = 6.58AAEE323 pKa = 4.45NNANEE328 pKa = 4.05IRR330 pKa = 11.84EE331 pKa = 4.01VLYY334 pKa = 10.83NYY336 pKa = 10.84SLDD339 pKa = 4.41DD340 pKa = 4.1YY341 pKa = 11.56AIDD344 pKa = 4.31DD345 pKa = 4.31HH346 pKa = 8.24NIDD349 pKa = 4.16GNAEE353 pKa = 3.96VQDD356 pKa = 3.68NHH358 pKa = 8.18ISDD361 pKa = 4.1NNTNEE366 pKa = 3.66ARR368 pKa = 11.84EE369 pKa = 4.09AQVNEE374 pKa = 3.98EE375 pKa = 4.29LDD377 pKa = 3.99NVNTTANTQNINEE390 pKa = 4.34RR391 pKa = 11.84LEE393 pKa = 4.31HH394 pKa = 5.71CHH396 pKa = 6.68ISQTNLHH403 pKa = 6.11KK404 pKa = 10.17HH405 pKa = 5.4TFQKK409 pKa = 11.07

Molecular weight: 45.86 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>sp|Q2GH34|RS11_EHRCR 30S ribosomal protein S11 OS=Ehrlichia chaffeensis (strain ATCC CRL-10679 / Arkansas) OX=205920 GN=rpsK PE=3 SV=1
MM1 pKa = 7.71KK2 pKa = 9.6GTFQPSRR9 pKa = 11.84IVRR12 pKa = 11.84KK13 pKa = 9.02RR14 pKa = 11.84RR15 pKa = 11.84HH16 pKa = 4.86GFRR19 pKa = 11.84SRR21 pKa = 11.84MSTKK25 pKa = 9.02MGRR28 pKa = 11.84RR29 pKa = 11.84ILNRR33 pKa = 11.84RR34 pKa = 11.84RR35 pKa = 11.84AQGRR39 pKa = 11.84RR40 pKa = 11.84VLCAA44 pKa = 3.56

Molecular weight: 5.31 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

1100

0

1100

310362

30

4313

282.1

31.8

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

5.37 ± 0.067

1.669 ± 0.04

5.434 ± 0.101

5.495 ± 0.087

4.541 ± 0.075

5.501 ± 0.067

2.224 ± 0.036

9.747 ± 0.131

7.233 ± 0.069

9.551 ± 0.086

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.457 ± 0.039

6.499 ± 0.091

3.021 ± 0.043

3.327 ± 0.049

3.532 ± 0.052

8.039 ± 0.092

4.865 ± 0.064

6.876 ± 0.078

0.652 ± 0.023

3.965 ± 0.062
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski