Vibrio phage JA-1

Taxonomy: Viruses; Duplodnaviria; Heunggongvirae; Uroviricota; Caudoviricetes; Caudovirales; Schitoviridae; Pacinivirus; unclassified Pacinivirus

Average proteome isoelectric point is 6.58

Get precalculated fractions of proteins

Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
    
All



Virtual 2D-PAGE plot for 79 proteins (isoelectric point calculated using IPC2_protein)

Get csv file with sequences according to given criteria:
        -     Method 

     -  kDa    
                                                                                      

* You can choose from 21 different methods for calculating isoelectric point

Summary statistics related to proteome-wise predictions


    
Protein with the lowest isoelectric point:
>tr|R9R5B6|R9R5B6_9CAUD Uncharacterized protein OS=Vibrio phage JA-1 OX=1283071 GN=JA1_0061 PE=4 SV=1
MM1 pKa = 8.09DD2 pKa = 3.99NHH4 pKa = 6.95PFFIMGLFSKK14 pKa = 9.33TRR16 pKa = 11.84IYY18 pKa = 10.39TYY20 pKa = 10.43SQTVSLIEE28 pKa = 4.09DD29 pKa = 3.7TPDD32 pKa = 3.19VVKK35 pKa = 11.02QSVTNSILGNKK46 pKa = 9.21EE47 pKa = 3.52IVPNLMDD54 pKa = 3.32TTLNRR59 pKa = 11.84LSSRR63 pKa = 11.84VNRR66 pKa = 11.84YY67 pKa = 8.39YY68 pKa = 10.98KK69 pKa = 10.75YY70 pKa = 10.83GITDD74 pKa = 4.07FTNGLPEE81 pKa = 4.17GSFGYY86 pKa = 10.42FKK88 pKa = 11.14ADD90 pKa = 3.1ADD92 pKa = 3.91AVIDD96 pKa = 3.67VLTNHH101 pKa = 6.03VLTLQPNEE109 pKa = 4.1EE110 pKa = 4.21VSLISYY116 pKa = 9.09VIGEE120 pKa = 4.54INPAFYY126 pKa = 10.56GFKK129 pKa = 9.74WLRR132 pKa = 11.84GNTDD136 pKa = 2.33WNYY139 pKa = 10.32KK140 pKa = 10.08DD141 pKa = 4.8NYY143 pKa = 7.83VTTVPGIGTNNLVYY157 pKa = 10.8AGFDD161 pKa = 3.33VLSEE165 pKa = 4.01NLIKK169 pKa = 11.17LNFNEE174 pKa = 4.26QNDD177 pKa = 4.33LSPEE181 pKa = 4.04PNVYY185 pKa = 10.44SITIPAVPSITDD197 pKa = 3.18EE198 pKa = 4.2LYY200 pKa = 11.56YY201 pKa = 10.2MVTYY205 pKa = 10.88NIVDD209 pKa = 3.86KK210 pKa = 10.28DD211 pKa = 3.84TEE213 pKa = 4.15DD214 pKa = 5.03AIGNPYY220 pKa = 9.1YY221 pKa = 10.16WIYY224 pKa = 11.0NAAEE228 pKa = 4.63DD229 pKa = 4.34IYY231 pKa = 11.69VEE233 pKa = 4.33LKK235 pKa = 10.43LPEE238 pKa = 3.99YY239 pKa = 10.64SKK241 pKa = 9.94YY242 pKa = 8.0TQYY245 pKa = 11.62LPIVPVRR252 pKa = 11.84IQNQDD257 pKa = 3.13MLDD260 pKa = 3.88PNLTTDD266 pKa = 4.65LYY268 pKa = 8.87RR269 pKa = 11.84TSVKK273 pKa = 9.67ILKK276 pKa = 10.24YY277 pKa = 10.71VDD279 pKa = 3.01IDD281 pKa = 3.89ANQLRR286 pKa = 11.84EE287 pKa = 4.54SINASPDD294 pKa = 3.09IGSLDD299 pKa = 2.95HH300 pKa = 7.04AYY302 pKa = 10.28FVFGYY307 pKa = 8.23PIRR310 pKa = 11.84VEE312 pKa = 3.79SDD314 pKa = 2.86VGNEE318 pKa = 3.54YY319 pKa = 10.64LYY321 pKa = 10.74EE322 pKa = 4.09YY323 pKa = 10.85FNDD326 pKa = 3.24LHH328 pKa = 6.86DD329 pKa = 4.99RR330 pKa = 11.84NIFTKK335 pKa = 10.44TDD337 pKa = 3.46FDD339 pKa = 3.53NWFSSEE345 pKa = 4.35TEE347 pKa = 3.92KK348 pKa = 8.43TTPPVNVIQIEE359 pKa = 4.18DD360 pKa = 3.58DD361 pKa = 3.72TYY363 pKa = 9.75KK364 pKa = 10.54TKK366 pKa = 10.59IMYY369 pKa = 9.98YY370 pKa = 9.95YY371 pKa = 8.36ITRR374 pKa = 11.84EE375 pKa = 3.87IKK377 pKa = 10.41VGNLTKK383 pKa = 9.93IGKK386 pKa = 9.09VIKK389 pKa = 9.76EE390 pKa = 4.29VYY392 pKa = 10.16SGNQDD397 pKa = 2.56ITFEE401 pKa = 4.25NDD403 pKa = 2.64NVSYY407 pKa = 8.35TTEE410 pKa = 4.01SPDD413 pKa = 3.23NRR415 pKa = 11.84VVLKK419 pKa = 10.73KK420 pKa = 10.61QITTNTYY427 pKa = 9.79SEE429 pKa = 4.89LIITGLVHH437 pKa = 6.78FNNIYY442 pKa = 10.26GVNRR446 pKa = 11.84DD447 pKa = 4.2EE448 pKa = 4.54ITTLDD453 pKa = 3.65SANTEE458 pKa = 4.15SFIIPLNISIINQMKK473 pKa = 10.17LWDD476 pKa = 4.67RR477 pKa = 11.84DD478 pKa = 3.51DD479 pKa = 5.75LYY481 pKa = 11.62YY482 pKa = 11.03KK483 pKa = 10.62CFNLVLNAYY492 pKa = 8.95EE493 pKa = 4.31KK494 pKa = 10.63IKK496 pKa = 10.73LKK498 pKa = 9.99WYY500 pKa = 6.68QTGIFKK506 pKa = 10.73VFTIVLVIAISIYY519 pKa = 10.02TGGAASFITGLISAATAGVISLGIFIAEE547 pKa = 4.96LYY549 pKa = 7.6LTSYY553 pKa = 8.62VFKK556 pKa = 11.3YY557 pKa = 10.49SFEE560 pKa = 4.24KK561 pKa = 10.54VAEE564 pKa = 4.01ALGIDD569 pKa = 3.96LAFVLALVLVIGGSIASLNSLPNADD594 pKa = 3.02IFLNVGTGLGQGVGQVIQEE613 pKa = 4.1EE614 pKa = 4.57AIGIQDD620 pKa = 4.14EE621 pKa = 4.46LTALQEE627 pKa = 4.19YY628 pKa = 10.38YY629 pKa = 10.95GLKK632 pKa = 9.92FDD634 pKa = 4.83EE635 pKa = 4.87LFSVQEE641 pKa = 3.87EE642 pKa = 4.5LQTNKK647 pKa = 9.86TNILDD652 pKa = 4.67PIGLYY657 pKa = 9.93TDD659 pKa = 3.23IGMFPNEE666 pKa = 4.16SSEE669 pKa = 4.19MYY671 pKa = 9.92LQRR674 pKa = 11.84KK675 pKa = 8.67IEE677 pKa = 3.99LVNPGILTIDD687 pKa = 3.35AVSNFVDD694 pKa = 3.46NMLTLRR700 pKa = 11.84TSS702 pKa = 3.03

Molecular weight: 79.57 kDa
Isoelectric point according different methods:





Protein with the highest isoelectric point:
>tr|R9R4F7|R9R4F7_9CAUD PF10947 family protein OS=Vibrio phage JA-1 OX=1283071 GN=JA1_0018 PE=4 SV=1
MM1 pKa = 7.64KK2 pKa = 10.5LLNVWVHH9 pKa = 4.71PTEE12 pKa = 4.46PEE14 pKa = 3.75SWFHH18 pKa = 5.35YY19 pKa = 9.88FKK21 pKa = 11.34VNNQVIRR28 pKa = 11.84FIPSPKK34 pKa = 9.75HH35 pKa = 6.38RR36 pKa = 11.84INNCPLRR43 pKa = 11.84IIPNHH48 pKa = 5.56FVKK51 pKa = 10.53LDD53 pKa = 3.43KK54 pKa = 10.92QLTVLRR60 pKa = 11.84TVV62 pKa = 3.24

Molecular weight: 7.5 kDa
Isoelectric point according different methods:





Peptides (in silico digests for buttom-up proteomics)

Below you can find in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.

Try
ESI
ChTry
ESI
ArgC
ESI
LysN
ESI
TryLysC
ESI

Try
MALDI
ChTry
MALDI
ArgC
MALDI
LysN
MALDI
TryLysC
MALDI

Try
LTQ
ChTry
LTQ
ArgC
LTQ
LysN
LTQ
TryLysC
LTQ

Try
MSlow
ChTry
MSlow
ArgC
MSlow
LysN
MSlow
TryLysC
MSlow

Try
MShigh
ChTry
MShigh
ArgC
MShigh
LysN
MShigh
TryLysC
MShigh

General Statistics

Number of major isoforms

Number of additional isoforms

Number of all proteins

Number of amino acids

Min. Seq. Length

Max. Seq. Length

Avg. Seq. Length

Avg. Mol. Weight

79

0

79

18339

37

3022

232.1

26.32

Amino acid frequency

Ala

Cys

Asp

Glu

Phe

Gly

His

Ile

Lys

Leu

6.587 ± 0.428

0.878 ± 0.174

5.595 ± 0.2

7.307 ± 0.34

4.03 ± 0.167

5.595 ± 0.289

1.865 ± 0.232

6.587 ± 0.313

7.879 ± 0.273

8.91 ± 0.228

Met

Asn

Gln

Pro

Arg

Ser

Thr

Val

Trp

Tyr

2.585 ± 0.179

6.304 ± 0.178

3.659 ± 0.162

4.051 ± 0.274

3.79 ± 0.167

6.205 ± 0.283

6.429 ± 0.173

6.347 ± 0.284

1.14 ± 0.159

4.259 ± 0.282
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level

Most of the basic statistics you can see at this page can be downloaded from this CSV file

For dipeptide frequency statistics click here
See this proteome in: uniprot_link
Proteome-pI is available under Creative Commons Attribution-NoDerivs license, for more details see here

Reference: Kozlowski LP. Proteome-pI 2.0: Proteome Isoelectric Point Database Update. Nucleic Acids Res. 2021, doi: 10.1093/nar/gkab944 Contact: Lukasz P. Kozlowski