Tursiops truncatus (Atlantic bottle-nosed dolphin) (Delphinus truncatus)
Average proteome isoelectric point is 6.69
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 45130 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|A0A6J3PZ46|A0A6J3PZ46_TURTR Isoform of A0A6J3PZC9 transcription elongation factor A protein 2 isoform X2 OS=Tursiops truncatus OX=9739 GN=TCEA2 PE=3 SV=1
MM1 pKa = 7.23 EE2 pKa = 5.18 AVSPVLFVLGLLLLPLLAVLLMALCVHH29 pKa = 6.24 CRR31 pKa = 11.84 EE32 pKa = 4.88 LPGSYY37 pKa = 8.67 DD38 pKa = 3.24 TAASDD43 pKa = 3.91 GLTPSSIVIKK53 pKa = 9.79 PPPTAASWPPATSYY67 pKa = 11.28 PPVTYY72 pKa = 10.68 LLQSQPDD79 pKa = 3.91 LLRR82 pKa = 11.84 IPRR85 pKa = 11.84 SPQAPGGSPRR95 pKa = 11.84 MPSSQQDD102 pKa = 3.01 SDD104 pKa = 3.88 GANSVASYY112 pKa = 10.11 EE113 pKa = 4.13 NEE115 pKa = 4.2 GASGAPAALAGRR127 pKa = 11.84 RR128 pKa = 11.84 LGPVLGSADD137 pKa = 3.96 PVSLPTPEE145 pKa = 4.78 AVCEE149 pKa = 4.13 DD150 pKa = 3.73 ADD152 pKa = 3.94 EE153 pKa = 6.05 DD154 pKa = 4.37 EE155 pKa = 5.92 DD156 pKa = 4.86 EE157 pKa = 4.15 EE158 pKa = 5.22 DD159 pKa = 3.81 YY160 pKa = 11.56 PEE162 pKa = 5.32 GYY164 pKa = 10.61 LVVLPDD170 pKa = 3.71 NVPATGAAVPPAPASSNPGLRR191 pKa = 11.84 DD192 pKa = 3.06 SAFSMEE198 pKa = 4.23 SGEE201 pKa = 4.99 DD202 pKa = 3.63 YY203 pKa = 11.66 VNVPEE208 pKa = 4.68 SEE210 pKa = 4.29 EE211 pKa = 4.1 SADD214 pKa = 3.58 ASLDD218 pKa = 3.44 GSRR221 pKa = 11.84 EE222 pKa = 3.99 YY223 pKa = 11.72 VNVSQEE229 pKa = 4.24 LPPVARR235 pKa = 11.84 TEE237 pKa = 4.35 PGVCWGSQPEE247 pKa = 4.17 FPGGGGG253 pKa = 3.16
Molecular weight: 26.09 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.72
IPC2_protein 3.821
IPC_protein 3.795
Toseland 3.592
ProMoST 3.935
Dawson 3.757
Bjellqvist 3.91
Wikipedia 3.668
Rodwell 3.617
Grimsley 3.503
Solomon 3.745
Lehninger 3.706
Nozaki 3.872
DTASelect 4.05
Thurlkill 3.63
EMBOSS 3.681
Sillero 3.91
Patrickios 1.062
IPC_peptide 3.757
IPC2_peptide 3.884
IPC2.peptide.svr19 3.79
Protein with the highest isoelectric point:
>tr|A0A6J3QL83|A0A6J3QL83_TURTR homeobox protein Hox-B3 OS=Tursiops truncatus OX=9739 GN=HOXB3 PE=3 SV=1
MM1 pKa = 7.4 QSISCVLGKK10 pKa = 8.76 TPLPRR15 pKa = 11.84 QKK17 pKa = 10.47 SGSHH21 pKa = 4.93 RR22 pKa = 11.84 THH24 pKa = 7.41 LLTTAHH30 pKa = 6.41 SSVKK34 pKa = 10.25 SAAKK38 pKa = 9.94 SGSHH42 pKa = 5.28 RR43 pKa = 11.84 THH45 pKa = 7.13 LLATAHH51 pKa = 6.3 SSVKK55 pKa = 10.42 SVAKK59 pKa = 10.2 SGSHH63 pKa = 5.01 RR64 pKa = 11.84 THH66 pKa = 7.13 LLATAHH72 pKa = 6.3 SSVKK76 pKa = 10.42 SVAKK80 pKa = 10.2 SGSHH84 pKa = 4.95 RR85 pKa = 11.84 THH87 pKa = 7.41 LLTTAHH93 pKa = 6.41 SSVKK97 pKa = 10.25 SAAKK101 pKa = 9.94 SGSHH105 pKa = 5.28 RR106 pKa = 11.84 THH108 pKa = 7.13 LLATAHH114 pKa = 6.3 SSVKK118 pKa = 10.42 SVAKK122 pKa = 10.2 SGSHH126 pKa = 4.95 RR127 pKa = 11.84 THH129 pKa = 7.41 LLTTAHH135 pKa = 6.43 SSVKK139 pKa = 10.36 SVAKK143 pKa = 10.2 SGSHH147 pKa = 5.01 RR148 pKa = 11.84 THH150 pKa = 7.13 LLATAHH156 pKa = 6.33 SSVKK160 pKa = 10.3 SAAKK164 pKa = 9.94 SGSHH168 pKa = 5.28 RR169 pKa = 11.84 THH171 pKa = 7.13 LLATAHH177 pKa = 6.3 SSVKK181 pKa = 10.42 SVAKK185 pKa = 10.2 SGSHH189 pKa = 4.95 RR190 pKa = 11.84 THH192 pKa = 7.41 LLTTAHH198 pKa = 6.43 SSVKK202 pKa = 10.36 SVAKK206 pKa = 10.2 SGSHH210 pKa = 4.95 RR211 pKa = 11.84 THH213 pKa = 7.41 LLTTAHH219 pKa = 6.43 SSVKK223 pKa = 10.36 SVAKK227 pKa = 10.2 SGSHH231 pKa = 5.01 RR232 pKa = 11.84 THH234 pKa = 7.13 LLATAHH240 pKa = 6.3 SSVKK244 pKa = 10.42 SVAKK248 pKa = 10.2 SGSHH252 pKa = 5.01 RR253 pKa = 11.84 THH255 pKa = 7.13 LLATAHH261 pKa = 6.3 SSVKK265 pKa = 10.42 SVAKK269 pKa = 10.2 SGSHH273 pKa = 4.95 RR274 pKa = 11.84 THH276 pKa = 7.41 LLTTAHH282 pKa = 6.43 SSVKK286 pKa = 10.36 SVAKK290 pKa = 10.2 SGSHH294 pKa = 5.01 RR295 pKa = 11.84 THH297 pKa = 7.13 LLATAHH303 pKa = 6.3 SSVKK307 pKa = 10.42 SVAKK311 pKa = 10.2 SGSHH315 pKa = 4.95 RR316 pKa = 11.84 THH318 pKa = 7.41 LLTTAHH324 pKa = 6.43 SSVKK328 pKa = 10.36 SVAKK332 pKa = 10.2 SGSHH336 pKa = 4.95 RR337 pKa = 11.84 THH339 pKa = 7.41 LLTTAHH345 pKa = 6.43 SSVKK349 pKa = 10.32 SVAKK353 pKa = 10.24 RR354 pKa = 11.84 QVEE357 pKa = 3.94 DD358 pKa = 3.83 SIRR361 pKa = 11.84 LTFF364 pKa = 4.2
Molecular weight: 37.89 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.4
IPC2_protein 10.906
IPC_protein 12.413
Toseland 12.603
ProMoST 13.071
Dawson 12.618
Bjellqvist 12.588
Wikipedia 13.071
Rodwell 12.588
Grimsley 12.647
Solomon 13.086
Lehninger 12.983
Nozaki 12.603
DTASelect 12.588
Thurlkill 12.603
EMBOSS 13.1
Sillero 12.603
Patrickios 12.296
IPC_peptide 13.086
IPC2_peptide 12.062
IPC2.peptide.svr19 8.971
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
19119
26011
45130
31182114
28
34359
690.9
76.79
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
7.187 ± 0.012
2.11 ± 0.009
4.845 ± 0.007
7.335 ± 0.016
3.396 ± 0.008
6.584 ± 0.016
2.583 ± 0.005
4.047 ± 0.01
5.712 ± 0.014
9.902 ± 0.016
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.047 ± 0.005
3.448 ± 0.008
6.606 ± 0.021
4.99 ± 0.01
5.871 ± 0.012
8.574 ± 0.012
5.213 ± 0.008
5.926 ± 0.009
1.158 ± 0.004
2.454 ± 0.006
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here