Aurantimonas manganoxydans (strain ATCC BAA-1229 / DSM 21871 / SI85-9A1)
Average proteome isoelectric point is 6.3
Get precalculated fractions of proteins
Acidic
pI < 6.8
6.8-7.4
pI > 7.4
Basic
All
Note: above files contain also dissociation constants (pKa)
Virtual 2D-PAGE plot for 3625 proteins (isoelectric point calculated using IPC2_protein)
Get csv file with sequences according to given criteria:
* You can choose from 21 different methods for calculating isoelectric point
Summary statistics related to proteome-wise predictions
Protein with the lowest isoelectric point:
>tr|Q1YEJ7|Q1YEJ7_AURMS Putative exonuclease OS=Aurantimonas manganoxydans (strain ATCC BAA-1229 / DSM 21871 / SI85-9A1) OX=287752 GN=SI859A1_01078 PE=4 SV=1
MM1 pKa = 7.85 TGGQDD6 pKa = 3.5 MNIKK10 pKa = 10.36 SLLLGSAAALVAVSGARR27 pKa = 11.84 AADD30 pKa = 3.68 VIMPVAPEE38 pKa = 3.71 PVDD41 pKa = 3.52 YY42 pKa = 11.42 VRR44 pKa = 11.84 VCDD47 pKa = 3.88 VYY49 pKa = 10.81 GTGFYY54 pKa = 10.01 YY55 pKa = 9.98 IPGTEE60 pKa = 3.92 TCLSINGYY68 pKa = 8.74 VRR70 pKa = 11.84 FQYY73 pKa = 10.71 NVAGGIEE80 pKa = 4.43 DD81 pKa = 5.31 DD82 pKa = 4.58 FGNDD86 pKa = 4.01 LLTNSDD92 pKa = 3.37 GDD94 pKa = 3.77 NYY96 pKa = 10.59 QAGSVVRR103 pKa = 11.84 VRR105 pKa = 11.84 LNFDD109 pKa = 2.97 ARR111 pKa = 11.84 EE112 pKa = 3.94 EE113 pKa = 4.57 TEE115 pKa = 4.58 LGTLRR120 pKa = 11.84 AYY122 pKa = 10.59 ARR124 pKa = 11.84 VQAQNAGGAYY134 pKa = 10.65 SNDD137 pKa = 2.71 ASYY140 pKa = 11.86 NMDD143 pKa = 3.2 MGFIQLGGLTMGYY156 pKa = 10.52 LDD158 pKa = 4.17 TLWTEE163 pKa = 4.38 GDD165 pKa = 3.77 GLLTDD170 pKa = 3.6 TDD172 pKa = 4.77 LPVGDD177 pKa = 4.24 YY178 pKa = 7.2 QTNRR182 pKa = 11.84 ISYY185 pKa = 8.18 TYY187 pKa = 10.27 AANGFTAALSLEE199 pKa = 4.47 DD200 pKa = 4.45 DD201 pKa = 3.67 ATGNFAPDD209 pKa = 3.32 VVGRR213 pKa = 11.84 LAYY216 pKa = 10.19 AGGWGSVYY224 pKa = 10.65 LAGSYY229 pKa = 10.52 DD230 pKa = 3.68 EE231 pKa = 4.99 QVDD234 pKa = 3.96 GRR236 pKa = 11.84 LLTPASFTAAAASGLQVNSAGVIVPASATFVSGIDD271 pKa = 3.34 PTTDD275 pKa = 3.08 DD276 pKa = 4.15 GAFALKK282 pKa = 10.53 AGLSLTDD289 pKa = 5.44 LIAADD294 pKa = 4.24 SEE296 pKa = 4.54 LKK298 pKa = 10.46 IEE300 pKa = 4.32 GSYY303 pKa = 11.35 AFDD306 pKa = 4.1 PSSYY310 pKa = 9.69 STIGLFSNRR319 pKa = 11.84 ATLGTQLDD327 pKa = 4.97 LLTGTLPVEE336 pKa = 4.11 WQIGAGYY343 pKa = 9.37 AQQFGKK349 pKa = 10.79 LGVAVSGVYY358 pKa = 10.82 GEE360 pKa = 4.52 TFDD363 pKa = 3.68 TYY365 pKa = 10.95 RR366 pKa = 11.84 YY367 pKa = 8.03 TNATTFSNRR376 pKa = 11.84 GDD378 pKa = 3.37 GEE380 pKa = 4.46 YY381 pKa = 10.82 YY382 pKa = 10.8 KK383 pKa = 11.07 LVGNVGYY390 pKa = 10.47 EE391 pKa = 3.6 ITNNFDD397 pKa = 3.11 MLAEE401 pKa = 4.15 ISYY404 pKa = 11.49 ANIDD408 pKa = 3.46 FDD410 pKa = 4.09 GQNGFVDD417 pKa = 3.57 NSLDD421 pKa = 3.41 QTAGFLRR428 pKa = 11.84 FVRR431 pKa = 11.84 SFF433 pKa = 3.04
Molecular weight: 45.97 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 3.776
IPC2_protein 3.821
IPC_protein 3.859
Toseland 3.617
ProMoST 4.024
Dawson 3.846
Bjellqvist 3.999
Wikipedia 3.795
Rodwell 3.668
Grimsley 3.528
Solomon 3.846
Lehninger 3.808
Nozaki 3.961
DTASelect 4.228
Thurlkill 3.681
EMBOSS 3.808
Sillero 3.973
Patrickios 0.502
IPC_peptide 3.846
IPC2_peptide 3.948
IPC2.peptide.svr19 3.858
Protein with the highest isoelectric point:
>tr|Q1YI37|Q1YI37_AURMS Acyl-[acyl-carrier-protein]--UDP-N-acetylglucosamine O-acyltransferase OS=Aurantimonas manganoxydans (strain ATCC BAA-1229 / DSM 21871 / SI85-9A1) OX=287752 GN=lpxA PE=3 SV=1
MM1 pKa = 6.75 TAAIRR6 pKa = 11.84 AAASWISWAVFPSSRR21 pKa = 11.84 RR22 pKa = 11.84 LPLAAPRR29 pKa = 11.84 PRR31 pKa = 11.84 RR32 pKa = 11.84 HH33 pKa = 6.43 RR34 pKa = 11.84 SPPTHH39 pKa = 6.94 RR40 pKa = 11.84 ARR42 pKa = 11.84 LRR44 pKa = 11.84 RR45 pKa = 11.84 CPTRR49 pKa = 11.84 HH50 pKa = 5.7 RR51 pKa = 11.84 RR52 pKa = 11.84 PRR54 pKa = 11.84 PARR57 pKa = 11.84 PPRR60 pKa = 11.84 LRR62 pKa = 11.84 RR63 pKa = 11.84 SRR65 pKa = 11.84 HH66 pKa = 4.15 RR67 pKa = 11.84 RR68 pKa = 11.84 PRR70 pKa = 11.84 KK71 pKa = 9.03 FPHH74 pKa = 6.25 RR75 pKa = 11.84 PPRR78 pKa = 11.84 QHH80 pKa = 7.44 LSRR83 pKa = 11.84 KK84 pKa = 8.6 FLPTRR89 pKa = 11.84 PRR91 pKa = 11.84 MHH93 pKa = 7.06 RR94 pKa = 11.84 ASKK97 pKa = 8.29 TRR99 pKa = 11.84 RR100 pKa = 11.84 PFRR103 pKa = 11.84 PSRR106 pKa = 11.84 SRR108 pKa = 11.84 PQARR112 pKa = 11.84 RR113 pKa = 11.84 DD114 pKa = 3.58 RR115 pKa = 11.84 PTSRR119 pKa = 11.84 PRR121 pKa = 11.84 RR122 pKa = 11.84 RR123 pKa = 11.84 TASSASFAHH132 pKa = 6.65
Molecular weight: 15.88 kDa
Isoelectric point according different methods:
IPC2.protein.svr19 9.527
IPC2_protein 11.33
IPC_protein 12.939
Toseland 13.1
ProMoST 13.598
Dawson 13.1
Bjellqvist 13.1
Wikipedia 13.583
Rodwell 12.632
Grimsley 13.144
Solomon 13.598
Lehninger 13.51
Nozaki 13.1
DTASelect 13.1
Thurlkill 13.1
EMBOSS 13.598
Sillero 13.1
Patrickios 12.34
IPC_peptide 13.612
IPC2_peptide 12.603
IPC2.peptide.svr19 9.269
Peptides (in silico digests for buttom-up proteomics)
Below you can find
in silico digests of the whole proteome with Trypsin, Chymotrypsin, Trypsin+LysC, LysN, ArgC proteases suitable for different mass spec machines.
Try ESI
ChTry ESI
ArgC ESI
LysN ESI
TryLysC ESI
Try MALDI
ChTry MALDI
ArgC MALDI
LysN MALDI
TryLysC MALDI
Try LTQ
ChTry LTQ
ArgC LTQ
LysN LTQ
TryLysC LTQ
Try MSlow
ChTry MSlow
ArgC MSlow
LysN MSlow
TryLysC MSlow
Try MShigh
ChTry MShigh
ArgC MShigh
LysN MShigh
TryLysC MShigh
General Statistics
Number of major isoforms
Number of additional isoforms
Number of all proteins
Number of amino acids
Min. Seq. Length
Max. Seq. Length
Avg. Seq. Length
Avg. Mol. Weight
3625
0
3625
1230513
62
3297
339.5
36.67
Amino acid frequency
Ala
Cys
Asp
Glu
Phe
Gly
His
Ile
Lys
Leu
12.995 ± 0.056
0.86 ± 0.011
6.082 ± 0.036
5.842 ± 0.039
3.69 ± 0.025
8.941 ± 0.038
1.971 ± 0.021
5.125 ± 0.031
2.767 ± 0.034
9.671 ± 0.053
Met
Asn
Gln
Pro
Arg
Ser
Thr
Val
Trp
Tyr
2.54 ± 0.019
2.339 ± 0.022
5.22 ± 0.032
2.86 ± 0.025
7.622 ± 0.044
5.37 ± 0.034
5.393 ± 0.027
7.42 ± 0.029
1.207 ± 0.015
2.084 ± 0.018
Note: For amino acid frequency statistics the error has been estimated with the bootstraping (x100) at the protein level
Most of the basic statistics you can see at this page can be downloaded from this CSV file
For dipeptide frequency statistics click here